STUDY AND APPLICATION OF BELT MULTI-POINT PULSE THERAPEUTIC APPARATUS

We developed Belt Multiple Point Pulse Thenapeutic Apparatus for curinglumbo-muscle fatigue, arthritis, productive osteosis and various soft tissue injuries. Itis based on the principle of acupuncture and moxibustion of traditional Chinese medicineand we combined it with healthy recovery medicine, moddern electornic technology.Such apparatus Possesses rollowing propertiesl no wound ; no surfering ; no cross-inrection and fainting spell during acupuncture treatment, heing portablo and easy to operate. Having been applied for more than one year in clinical practice, this apparatusproves to be safe and reliable, stable in its properties. The excellent curatitve ratereached 65%. The general erficiency reached more than 90%. So it can be widely usedboth for therapeutic and hygiene in hospitals and households.

Nanobodies with cross-neutralizing activity provide prominent therapeutic efficacy in mild and severe COVID-19 rodent models

The weakened protective efficacy of COVID-19 vaccines and antibodies caused by SARS-CoV-2 variants presents a global health emergency,which underscores the urgent need for universal therapeutic antibody intervention for clinical patients.Here,we screened three alpacas-derived nanobodies(Nbs)with neutralizing activity from twenty RBD-specific Nbs.The three Nbs were fused with the Fc domain of human IgG,namely aVHH-11-Fc,aVHH-13-Fc and aVHH-14-Fc,which could specifically bind RBD protein and competitively inhibit the binding of ACE2 receptor to RBD.They effectively neutralized SARS-CoV-2 pseudoviruses D614G,Alpha,Beta,Gamma,Delta,and Omicron sub-lineages BA.1,BA.2,BA.4,and BA.5 and authentic SARS-CoV-2 prototype,Delta,and Omicron BA.1,BA.2 strains.In mice-adapted COVID-19 severe model,intranasal administration of aVHH-11-Fc,aVHH-13-Fc and aVHH-14-Fc effectively protected mice from lethal challenges and reduced viral loads in both the upper and lower respiratory tracts.In the COVID-19 mild model,aVHH-13-Fc,which represents the optimal neutralizing activity among the above three Nbs,effectively protected hamsters from the challenge of SARS-CoV-2 prototype,Delta,Omicron BA.1 and BA.2 by significantly reducing viral replication and pathological alterations in the lungs.In structural modeling of aVHH-13 and RBD,aVHH-13 binds to the receptor-binding motif region of RBD and interacts with some highly conserved epitopes.Taken together,our study illustrated that alpaca-derived Nbs offered a therapeutic countermeasure against SARS-CoV-2,including those Delta and Omicron variants which have evolved into global pandemic strains.

The host-targeting compound peruvoside has a broad-spectrum antiviral activity against positive-sense RNA viruses

Positive-sense RNA viruses modify intracellular calcium stores,endoplasmic reticulum and Golgi apparatus(Golgi)to generate membranous replication organelles known as viral factories.Viral factories provide a conducive and substantial enclave for essential virus replication via concentrating necessary cellular factors and viral proteins in proximity.Here,we identified the vital role of a broadspectrum antiviral,peruvoside in limiting the formation of viral factories.Mechanistically,we revealed the pleiotropic cellular effect of Src and PLC kinase signaling via cyclin-dependent kinase 1 signaling leads to Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1(GBF1)phosphorylation and Golgi vesiculation by peruvoside treatment.The ramification of GBF1 phosphorylation fosters GBF1 deprivation consequentially activating downstream antiviral signaling by dampening viral factories formation.Further investigation showed signaling of ERK1/2 pathway via cyclin-dependent kinase 1 activation leading to GBF1 phosphorylation at Threonine 1337(T1337).We also showed 100%of protection in peruvoside-treated mouse model with a significant reduction in viral titre and without measurable cytotoxicity in serum.These findings highlight the importance of dissecting the broad-spectrum antiviral therapeutics mechanism and pave the way for consideration of peruvoside,host-directed antivirals for positive-sense RNA virus-mediated disease,in the interim where no vaccine is available.

Non-Invasive Treatment to Grade 1 Essential Hypertension by Percutaneous Laser and Electric Pulse to Acupoint with Music:A Randomized Controlled Trial

Objective： To study a non-drug therapy for hypertension disease by combining percutaneous laser and electric pulse stimulation to acupoint with music, and to test the efficiency of the combining treatment to grade 1 essential hypertension. Methods： A total of 174 patients with grade 1 essential hypertension were randomly assigned to 3 groups with a random number table after Chinese medicine（CM） syndrome differentiation： the photoelectric and musical treatment group（Group 1, with a self-developed multi-mode audio frequency pulse photoelectric therapeutic apparatus）, acupuncture group（Group 2）, and oral placebo group（Group 3）, 58 cases per group. The curative effect of each group was evaluated by the changes of blood pressure and CM syndrome integral before and after treatment. Results： Compared with Group 3, there were significant decrease of blood pressure and CM syndrome integral in Group 1 and Group 2（P〈0.01）. Compared with Group 2, Group 1 showed the highest decrease in systolic pressure（P〈0.017）. The total effective rate of anti-hypertension in Group 1（91.38%, 53/58） was significantly higher than that in Group 2（74.13%, 43/58） and Group 3（18.97%, 11/58, P〈0.05 or P〈0.01）; and that in Group 2 was also significantly higher than that in Group 3（P〈0.01）. There were significant difference in the total effective rate of CM syndrome integral in both Group 1（93.10%, 54/58） and Group 2（84.48%, 49/58） as compared with Group 3（17.24%, 10/58, P〈0.01）, while there was no significant difference between Group 1 and Group 2（P〉0.05）. Conclusions： The multi-mode audio frequency pulse photoelectric therapeutic apparatus, combining music, laser and electric pulse stimulation, is clinically useful for grade 1 essential hypertension. This ＂three in one＂ therapy method is non-invasive, easy and simple to handle. It is expected to be popularized as a new alternative treatment.

电针治疗仪在针灸治疗中的常规操作使用规范及适用范围

针对目前临床上对电子针灸治疗仪的广泛应用，却没有其使用规范，故通过对，晦床上电针治疗仪使用的疏理，总结出电针治疗仪的临床操作规范，如波型的选择、使用、适用范围、适应症、禁忌症等，以期更好地指导针灸，陆床治疗，提高陆床疗效，减少医疗事故及医疗纠纷。