Substitution of silkworm pupae （Bombyx mori） for fish meal in broodstock diets for snakeskin gourami （Trichogaster pectoralis） was conducted in order to evaluate the effect of silkworm pupae on feed digestibility ...Substitution of silkworm pupae （Bombyx mori） for fish meal in broodstock diets for snakeskin gourami （Trichogaster pectoralis） was conducted in order to evaluate the effect of silkworm pupae on feed digestibility and broodstock performance. Snakeskin gourami broodfish were fed with isocaloric diets （DE 3,000 a： 50 Kcal/kg） contained 32% crude protein. The levels of substitution were 0%, 50%, 75% and 100% of protein from fish meal （equal to 0%, 14.57%, 21.75% and 29.03% silk worm by weight）. The study was assigned in CRD with 4 treatments and 3 replications. Six hundred of snakeskin gourami with five-month old and average weight of 78.67 ± 2.15 g in female and average weight of 73.00 ± 2.49 g in male were stocked in 12 cages. Fifty fishes of 25 females and 25 males were reared in each cage and fed experimental diet for five months. The result indicated that protein digestibility of test diets were significantly decrease （P 〈 0.05） according to the level of silkworm pupae in diets where as the dry matter digestibility showed no significantly different （P 〉 0.05）. The growth performance and egg fecundity were significantly decreased （P 〈 0.05） according to the levels of silkworm pupae. In contrast, egg quality in term of fry number on first spawned, fingering number on first spawned were not significantly different （P 〉 0.05）. The survival rate of one month nursing fish on first spawned were significantly different （P 〈 0.05）. Hence, silkworm pupae could substitution for 50% of protein from fish meal （14.57% by weight） in snakeskin gourami broodstock diets without any adverse effect on egg quality.展开更多
Patinopecten yessoensis is a commercial valuable species. This study deals with the effect of nutrient effects of the broodstock(mainly ovaries) on the larvae. Concentrations of total carbohydrate, total protein and t...Patinopecten yessoensis is a commercial valuable species. This study deals with the effect of nutrient effects of the broodstock(mainly ovaries) on the larvae. Concentrations of total carbohydrate, total protein and total lipid in the gonads of P. yessoensis from three Hatcheries(Hatchery 1, Hatchery 2, and Hatchery 3) were determined before and after spawning. The relationship between the nutrient concentration in ovaries before spawning(BC) and that of larvae(LC) was assessed as well as the change in nutrient levels in ovaries after spawning(DC). Results indicate that the BC of total carbohydrate(7.66%) and total lipid(14.48%) in ovaries were significantly higher than in testes(5.20%, 5.20% respectively), whereas the BC of total protein in the ovaries was lower(61.76%) than in the testes(81.67%). The different gonadal composition suggests the different nutrient demands between male and female broodstocks in breeding season. P atinopecten yessoensis gonads contained a higher proportion of lipids, in comparison to other bivalves, which might be a response to the low ambient water temperatures. Further analysis of fatty acids showed that the concentrations of n-3PUFA, EPA and DHA in larvae(LC) were positively correlated with BC and DC, indicating the significant nutrient influence of broodstocks on the larvae. As these fatty acids are important in metabolism, and have been demonstrated to be influential to the viability of the larvae, larval growth and the settlement, spat growth, and juvenile survival in many bivalves, they could possibly be used as indexes to evaluate, and predict condition of broodstocks and larvae.展开更多
The present trial was conducted with starry flounder Platichthys stellatus broodstock to evaluate the effects of dietary vitamin A, ascorbic acid, and a-tocopherol on the gonadal development and reproductive performan...The present trial was conducted with starry flounder Platichthys stellatus broodstock to evaluate the effects of dietary vitamin A, ascorbic acid, and a-tocopherol on the gonadal development and reproductive performance. 8 000 IU/kg diet vitaminA （VA group）, 500 mg/kg diet ascorbic acid （Vcpp group）, or 250 mg/kg diet a-tocopherol （a-TA group） was added into basal diet to create 3 vitamin experimental diets, respectively. Each diet was fed to 450 starry flounder broodstock for 104 days. Samples were collected weekly. The gonadosomatic index （GSI） of 3 vitamin experimental groups first decreased and then increased. Maximum GSI of Vcpp group was higher than that of ct-TA group but lower than that of VA group. The spawning periods of 3 vitamin experimental groups lasted 49, 56, and 45 days, respectively. No mature eggs were observed in the control group during the trial. The absolute fecundity （AF） and relative fecundity （RF） of a-TA group was higher than that of Vcpp group but lower than that of VA group. The results suggest that different vitamins play different roles in the fish reproductive process. Vitamin A stimulated the maturation of the ovary, ascorbic acid prolonged the spawning period, and a-tocopherol affected the development of the eggs.展开更多
The Tbx family is first known through the study of their functions in the body and limbs,and its members Tbx4 and Tbx5 genes are important factors in determining the characteristics of the appendages.Pampus argenteus ...The Tbx family is first known through the study of their functions in the body and limbs,and its members Tbx4 and Tbx5 genes are important factors in determining the characteristics of the appendages.Pampus argenteus is one of the important economical marine fishes widely distributed in offshore areas.Therefore,it is necessary to study the role of Tbx family genes in the deletion of pelvic fin in P.argenteus.In this study,we cloned Tbx4 and Tbx5 cDNA sequence of P.argenteus(GenBank:MH709128 and MH712458).The Western blot and real time PCR were used to detect the expressions of Tbx4 and Tbx5 in different developmental stages and tissues of P.argenteus.In addition,whole-mount in-situ hybridization was used to study the localization of Tbx4 and Tbx5 genes in different developmental stages of P.argenteus.Results show that the translation of Tbx4 mRNA was inhibited during the critical period of pelvic fin development.Among different tissues,Tbx4 protein levels were the lowest in the abdominal epithelium,and even lower than that in the pectoral fin,suggesting that the protein expression was also inhibited in the abdominal epithelium of adult P.argenteus.Therefore,the results indicated that upstream genes regulation led to the key stage-specific and low expression of Tbx4 during pelvic fin development and in the abdominal epithelium.展开更多
This paper investigated the lipid contents and fatty acid compositions of ovary, liver and muscle of the wild-caught broodstocks of Pampus cinereus in IV stage of ovary development with body weight from 408 g to 614 g...This paper investigated the lipid contents and fatty acid compositions of ovary, liver and muscle of the wild-caught broodstocks of Pampus cinereus in IV stage of ovary development with body weight from 408 g to 614 g from the East China Sea. The results indicated that there were significant differences of lipid contents and fatty acid compositions among ovary, liver or muscle tissues. The average lipid contents of ovary, liver and muscle of the broodstocks were 45.10%, 30.61% and 19.56% respectively. The lipid content in ovary was relatively stable while in liver and muscle was significantly variable among different broodstocks. There was a positive interrelation of lipid content between liver and muscle. The main fatty acids in ovary of the broodstock were as follows:C16:0>C22:6n3>C18:1n9>C16:4n3>C18:0. While C16:0>C18:1n9>C22:6n3>C18:0>C14:0 in muscle and C16:0>C18:1n9>C18:0>C22:6n3>C14:0 in liver respectively. The contents of DHA (22:6n3) and EPA (C22:5n3) of ovary, liver and muscle were 18.887% and 3.268%, 5.856% and 0.707% and 9.672% and 2.407% respectively. The compositions of C16:4n3,C22:6n3,PUFA,HUFA in ovary were significant higher than those in muscle and liver. The ratios of DHA/EPA/ARA in ovary, muscle and liver were 5.90/1/0.44, 4.03/1/0.50 and 8.51/1/0.85 respectively. The ratios of n-6PUFA/n-3PUFA in ovary, muscle and liver were 5.89, 3.51 and 4.67 respectively. Therefore these data will be useful for the artifical cultivation of the broodstocks of Pampus cinereus.展开更多
文摘Substitution of silkworm pupae （Bombyx mori） for fish meal in broodstock diets for snakeskin gourami （Trichogaster pectoralis） was conducted in order to evaluate the effect of silkworm pupae on feed digestibility and broodstock performance. Snakeskin gourami broodfish were fed with isocaloric diets （DE 3,000 a： 50 Kcal/kg） contained 32% crude protein. The levels of substitution were 0%, 50%, 75% and 100% of protein from fish meal （equal to 0%, 14.57%, 21.75% and 29.03% silk worm by weight）. The study was assigned in CRD with 4 treatments and 3 replications. Six hundred of snakeskin gourami with five-month old and average weight of 78.67 ± 2.15 g in female and average weight of 73.00 ± 2.49 g in male were stocked in 12 cages. Fifty fishes of 25 females and 25 males were reared in each cage and fed experimental diet for five months. The result indicated that protein digestibility of test diets were significantly decrease （P 〈 0.05） according to the level of silkworm pupae in diets where as the dry matter digestibility showed no significantly different （P 〉 0.05）. The growth performance and egg fecundity were significantly decreased （P 〈 0.05） according to the levels of silkworm pupae. In contrast, egg quality in term of fry number on first spawned, fingering number on first spawned were not significantly different （P 〉 0.05）. The survival rate of one month nursing fish on first spawned were significantly different （P 〈 0.05）. Hence, silkworm pupae could substitution for 50% of protein from fish meal （14.57% by weight） in snakeskin gourami broodstock diets without any adverse effect on egg quality.
基金Supported by the National Key Technology R&D Program of China(No.2011BAD13B05)the Agricultural Science and Technology Achievements Transformation Projects(No.2012GB24910656)+1 种基金the Special Program of Chinese Academy of Sciences for Strategic Advanced Science and Technology(No.XDA11020404)the National Special Research Fund for Non-Profit Marine Sector(No.201305043)
文摘Patinopecten yessoensis is a commercial valuable species. This study deals with the effect of nutrient effects of the broodstock(mainly ovaries) on the larvae. Concentrations of total carbohydrate, total protein and total lipid in the gonads of P. yessoensis from three Hatcheries(Hatchery 1, Hatchery 2, and Hatchery 3) were determined before and after spawning. The relationship between the nutrient concentration in ovaries before spawning(BC) and that of larvae(LC) was assessed as well as the change in nutrient levels in ovaries after spawning(DC). Results indicate that the BC of total carbohydrate(7.66%) and total lipid(14.48%) in ovaries were significantly higher than in testes(5.20%, 5.20% respectively), whereas the BC of total protein in the ovaries was lower(61.76%) than in the testes(81.67%). The different gonadal composition suggests the different nutrient demands between male and female broodstocks in breeding season. P atinopecten yessoensis gonads contained a higher proportion of lipids, in comparison to other bivalves, which might be a response to the low ambient water temperatures. Further analysis of fatty acids showed that the concentrations of n-3PUFA, EPA and DHA in larvae(LC) were positively correlated with BC and DC, indicating the significant nutrient influence of broodstocks on the larvae. As these fatty acids are important in metabolism, and have been demonstrated to be influential to the viability of the larvae, larval growth and the settlement, spat growth, and juvenile survival in many bivalves, they could possibly be used as indexes to evaluate, and predict condition of broodstocks and larvae.
基金Supported by the Public Science and Technology Research Funds Projects of Ocean(No.201205025)the Platform of Aquatic Nutrition and Feed(No.201304001)the Taishan Scholar Fellowship of Shandong Province
文摘The present trial was conducted with starry flounder Platichthys stellatus broodstock to evaluate the effects of dietary vitamin A, ascorbic acid, and a-tocopherol on the gonadal development and reproductive performance. 8 000 IU/kg diet vitaminA （VA group）, 500 mg/kg diet ascorbic acid （Vcpp group）, or 250 mg/kg diet a-tocopherol （a-TA group） was added into basal diet to create 3 vitamin experimental diets, respectively. Each diet was fed to 450 starry flounder broodstock for 104 days. Samples were collected weekly. The gonadosomatic index （GSI） of 3 vitamin experimental groups first decreased and then increased. Maximum GSI of Vcpp group was higher than that of ct-TA group but lower than that of VA group. The spawning periods of 3 vitamin experimental groups lasted 49, 56, and 45 days, respectively. No mature eggs were observed in the control group during the trial. The absolute fecundity （AF） and relative fecundity （RF） of a-TA group was higher than that of Vcpp group but lower than that of VA group. The results suggest that different vitamins play different roles in the fish reproductive process. Vitamin A stimulated the maturation of the ovary, ascorbic acid prolonged the spawning period, and a-tocopherol affected the development of the eggs.
基金Supported by the National Natural Science Foundation of China(Nos.31872586,42076118)the Major Project of Science,Technology and Innovation 2025 in Ningbo City(No.2021Z003)the K.C.Wong Magna Fund in Ningbo University。
文摘The Tbx family is first known through the study of their functions in the body and limbs,and its members Tbx4 and Tbx5 genes are important factors in determining the characteristics of the appendages.Pampus argenteus is one of the important economical marine fishes widely distributed in offshore areas.Therefore,it is necessary to study the role of Tbx family genes in the deletion of pelvic fin in P.argenteus.In this study,we cloned Tbx4 and Tbx5 cDNA sequence of P.argenteus(GenBank:MH709128 and MH712458).The Western blot and real time PCR were used to detect the expressions of Tbx4 and Tbx5 in different developmental stages and tissues of P.argenteus.In addition,whole-mount in-situ hybridization was used to study the localization of Tbx4 and Tbx5 genes in different developmental stages of P.argenteus.Results show that the translation of Tbx4 mRNA was inhibited during the critical period of pelvic fin development.Among different tissues,Tbx4 protein levels were the lowest in the abdominal epithelium,and even lower than that in the pectoral fin,suggesting that the protein expression was also inhibited in the abdominal epithelium of adult P.argenteus.Therefore,the results indicated that upstream genes regulation led to the key stage-specific and low expression of Tbx4 during pelvic fin development and in the abdominal epithelium.
文摘This paper investigated the lipid contents and fatty acid compositions of ovary, liver and muscle of the wild-caught broodstocks of Pampus cinereus in IV stage of ovary development with body weight from 408 g to 614 g from the East China Sea. The results indicated that there were significant differences of lipid contents and fatty acid compositions among ovary, liver or muscle tissues. The average lipid contents of ovary, liver and muscle of the broodstocks were 45.10%, 30.61% and 19.56% respectively. The lipid content in ovary was relatively stable while in liver and muscle was significantly variable among different broodstocks. There was a positive interrelation of lipid content between liver and muscle. The main fatty acids in ovary of the broodstock were as follows:C16:0>C22:6n3>C18:1n9>C16:4n3>C18:0. While C16:0>C18:1n9>C22:6n3>C18:0>C14:0 in muscle and C16:0>C18:1n9>C18:0>C22:6n3>C14:0 in liver respectively. The contents of DHA (22:6n3) and EPA (C22:5n3) of ovary, liver and muscle were 18.887% and 3.268%, 5.856% and 0.707% and 9.672% and 2.407% respectively. The compositions of C16:4n3,C22:6n3,PUFA,HUFA in ovary were significant higher than those in muscle and liver. The ratios of DHA/EPA/ARA in ovary, muscle and liver were 5.90/1/0.44, 4.03/1/0.50 and 8.51/1/0.85 respectively. The ratios of n-6PUFA/n-3PUFA in ovary, muscle and liver were 5.89, 3.51 and 4.67 respectively. Therefore these data will be useful for the artifical cultivation of the broodstocks of Pampus cinereus.
基金全球环境基金会/世界银行项目(GEF-MSP grant No.TF051795)云南省发展和改革委员会项目(滇中主要湖泊土著特有鱼类迁地保育人工繁殖与持续利用试验示范)+2 种基金中国科学院西部之光项目(292006312D11033)云南省科技厅社会发展科技计划项目(2008CA001)国家重点基础研究发展计划项目(2007CB411600)
