BACKGROUND Given the complex pathogenesis of ulcerative colitis (UC), the conventional therapeutic methods are not fully curative. As a sort of systematic complementary and alternative medicine, traditional Chinese me...BACKGROUND Given the complex pathogenesis of ulcerative colitis (UC), the conventional therapeutic methods are not fully curative. As a sort of systematic complementary and alternative medicine, traditional Chinese medicine (TCM) provides new options for the standard therapy. Nevertheless, there are still numerous problems with the promotion of TCM attributed to its complexity, and consequently, new research approaches are urgently needed. Thus, we explored the protective effects of Jian-Pi Qing-Chang (JPQC) decoction on UC based on systems pharmacology approach, which might fill the current innovation gap in drug discovery and clinical practice pertaining to TCM. AIM To investigate the protective mechanisms of JPQC decoction on UC based on systems pharmacology approach. METHODS We performed systems pharmacology to predict the active ingredients, the matched targets, and the potential pharmacological mechanism of JPQC on UC. In vivo, we explored the effects of JPQC in a colitis model induced by dextran sulfate sodium. In vitro, we adopted the bone marrow-derived macrophages (BMDMs) as well as BMDMs co-cultured with Caco2 cells to verify the underlying mechanisms and effects of JPQC on UC under TNF-α stimulation. RESULTS Systems pharmacology revealed 170 targets for the 107 active ingredients of JPQC and 112 candidate targets of UC. Protein-protein interaction networks were established to identify the underlying therapeutic targets of JPQC on UC. Based on enrichment analyses, we proposed our hypothesis that JPQC might have a protective effect on UC via the NF-κB/HIF-1α signalling pathway. Subsequent experimental validation revealed that treatment with TNFα activated the NF-κB/HIF-1α signalling pathway in BMDMs, thereby damaging the epithelial barrier permeability in co-cultured Caco2 cells, while JPQC rescued this situation. The findings were also confirmed in a dextran sulfate sodium-induced colitis model. CONCLUSION JPQC could improve the mucosal inflammatory response and intestinal epithelial barrier function via the NF-κB/HIF-1α signalling pathway, which provides new perspectives on the pharmaceutical development and clinical practice of TCM.展开更多
This study aimed to evaluate the efficacy of comprehensive therapy based on traditional Chinese medicine （TCM） patterns on older patients with chronic obstructive pulmonary disease （COPD） through a four- center, o...This study aimed to evaluate the efficacy of comprehensive therapy based on traditional Chinese medicine （TCM） patterns on older patients with chronic obstructive pulmonary disease （COPD） through a four- center, open-label, randomized controlled trial. Patients were divided into the trial group treated using conventional western medicine and Bu-Fei Jian-Pi granules, Bu-Fei Yi-Shen granules, and Yi-Qi Zi-Shen granules based on TCM patterns respectively; and the control group treated using conventional western medicine. A total of 136 patients ≥ 65 years completed the study, with 63 patients comprising the trial group and 73 comprising the control group. After the six-month treatment and the 12-month follow-up period, significant differences were observed between the trial and control groups in the following aspects： frequency of acute exacerbation （P ≤ 0.040）, duration of acute exacerbation （P = 0.034）, symptoms （P ≤ 0.034）, 6-min walking distance （6MWD） （P ≤ 0.039）, dyspnea scale （P ≤ 0.036）; physical domain （P ≤ 0.019）, psychological domain （P ≤ 0.033）, social domain （P ≤ 0.020）, and environmental domain （P ≤ 0.044） of the WHOQOL-BREF questionnaire; and daily living ability domain （P ≤ 0.007）, social activity domain （P ≤ 0.018）, depression symptoms domain （P ≤ 0.025）, and anxiety symptoms domain （P ≤ 0.037） of the COPD-QOL. No differences were observed between the trial and control groups with regard to FVC, FEV1, and FEV1%.展开更多
基金Supported by the National Natural Science Funds of China,No.81573892,No.81873253,and No.81704009
文摘BACKGROUND Given the complex pathogenesis of ulcerative colitis (UC), the conventional therapeutic methods are not fully curative. As a sort of systematic complementary and alternative medicine, traditional Chinese medicine (TCM) provides new options for the standard therapy. Nevertheless, there are still numerous problems with the promotion of TCM attributed to its complexity, and consequently, new research approaches are urgently needed. Thus, we explored the protective effects of Jian-Pi Qing-Chang (JPQC) decoction on UC based on systems pharmacology approach, which might fill the current innovation gap in drug discovery and clinical practice pertaining to TCM. AIM To investigate the protective mechanisms of JPQC decoction on UC based on systems pharmacology approach. METHODS We performed systems pharmacology to predict the active ingredients, the matched targets, and the potential pharmacological mechanism of JPQC on UC. In vivo, we explored the effects of JPQC in a colitis model induced by dextran sulfate sodium. In vitro, we adopted the bone marrow-derived macrophages (BMDMs) as well as BMDMs co-cultured with Caco2 cells to verify the underlying mechanisms and effects of JPQC on UC under TNF-α stimulation. RESULTS Systems pharmacology revealed 170 targets for the 107 active ingredients of JPQC and 112 candidate targets of UC. Protein-protein interaction networks were established to identify the underlying therapeutic targets of JPQC on UC. Based on enrichment analyses, we proposed our hypothesis that JPQC might have a protective effect on UC via the NF-κB/HIF-1α signalling pathway. Subsequent experimental validation revealed that treatment with TNFα activated the NF-κB/HIF-1α signalling pathway in BMDMs, thereby damaging the epithelial barrier permeability in co-cultured Caco2 cells, while JPQC rescued this situation. The findings were also confirmed in a dextran sulfate sodium-induced colitis model. CONCLUSION JPQC could improve the mucosal inflammatory response and intestinal epithelial barrier function via the NF-κB/HIF-1α signalling pathway, which provides new perspectives on the pharmaceutical development and clinical practice of TCM.
文摘This study aimed to evaluate the efficacy of comprehensive therapy based on traditional Chinese medicine （TCM） patterns on older patients with chronic obstructive pulmonary disease （COPD） through a four- center, open-label, randomized controlled trial. Patients were divided into the trial group treated using conventional western medicine and Bu-Fei Jian-Pi granules, Bu-Fei Yi-Shen granules, and Yi-Qi Zi-Shen granules based on TCM patterns respectively; and the control group treated using conventional western medicine. A total of 136 patients ≥ 65 years completed the study, with 63 patients comprising the trial group and 73 comprising the control group. After the six-month treatment and the 12-month follow-up period, significant differences were observed between the trial and control groups in the following aspects： frequency of acute exacerbation （P ≤ 0.040）, duration of acute exacerbation （P = 0.034）, symptoms （P ≤ 0.034）, 6-min walking distance （6MWD） （P ≤ 0.039）, dyspnea scale （P ≤ 0.036）; physical domain （P ≤ 0.019）, psychological domain （P ≤ 0.033）, social domain （P ≤ 0.020）, and environmental domain （P ≤ 0.044） of the WHOQOL-BREF questionnaire; and daily living ability domain （P ≤ 0.007）, social activity domain （P ≤ 0.018）, depression symptoms domain （P ≤ 0.025）, and anxiety symptoms domain （P ≤ 0.037） of the COPD-QOL. No differences were observed between the trial and control groups with regard to FVC, FEV1, and FEV1%.
