Pharmacological studies on Buchanania lanzan Spreng.-A focus on wound healing with particular reference to anti-biofilm properties

Objective:To evaluate the wound healing activity of the methanolic root extract of Buchanania lanzan Spreng.(B.lanzan),with a focus on antimicrobial and anti-biofilm properties.Methods:The extract was evaluated for its wound healing properties(excision and incision models)as evident from the analysis of tensile strength and wound contraction.The extract was also screened for antibacterial properties against different Cram positive and Gram negative bacterial strains.B.lanzan was also studied for its effect on biofilm formation and disruption of preformed biofilms.The synergistic effect of B.lanzan was determined in combination with gentamicin.Results:Topical application of B.lanzan(10%w/w ointment)significantly increased(40.84%)the tensile strength in the incision wound model.B.lanzan also showed significant wound healing activity in excision model and such significant activity was observed from the 9th day.Whereas Soframycin displayed significant wound healing activity from the 6th day.It was found that root extracts of B.lanzan revealed significant inhibition against all tested pathogens.B.lanzan displayed antimicrobial activity against Cram positive(MIC 0.625 mg/mL)and Cram negative(MIC0.625-1.25 mg/mL).B.lanzan was able to reduce biofilm formation and also caused disruption of preformed biofilms in a manner similar to ciprofloxacin.However,gentamicin was found to be ineffective against biofilms formed by Gram negative organism.According to the fractional inhibitory concentration index,B.lanzan displayed synergistic activity when it was combined with gentamicin.Conclusions:From this study it may be concluded that the root extract of B.lanzan revealed significant wound healing potential,which was supported and well correlated with pronounced antibacterial activity of the tested plant parts.

Effect of Buchanania lanzan Spreng.bark extract on cyclophosphamide induced genotoxicity and oxidative stress in mice

Objective:To elucidate the effect of ethanolic extract of Buchanania lanzan Spreng.(B.lanan) bark against cyclophosphamide induced genotoxicity and oxidative stress in mice.Methods: The prevalence of micronuclei in bone marrow,the extent of lipid peroxidation,reduced glutathione and the status of the antioxidant enzymes,superoxide dismutase and catalase in liver of mice were used as intermediate biomarkers for chemoprolection.Lipid peroxidation and associated compromised antioxidant defenses in cyclophosphamide treated mice were observed in the liver.Results:Pre-treatment with B.lanzan 250,500 and 1 000 mg/ kg,p.o.,daily for 7 days significantly reduced the chromosomal damage and lipid peroxidation with concomitant changes in antioxidants and detoxification systems.Conclusions:These results point out the presence of chemopreventive phytoconstituents in the crude extract offering protection against cyclophosphamide induced genotoxicity and oxidative stress in mice.

印度3种畅销植物药的开发利用

印度阿育吠陀传统医药体系历史悠久,博大精深,植物药在阿育吠陀医药理论中占有重要地位,印度药用植物约有7 263种,常用约960种。本文从资源分布、传统应用、化学成分和药理作用等方面对3种具有开发前景的印度畅销植物药——长叶紫荆木、兰桑山檨子及白背黄花稔进行介绍,为相关新药研发、中国亲缘相近的药用植物的开发利用、扩大中国药用植物资源及引种新资源提供参考。