The ill effects of green revolution include residues of extensively used chemical pesticides in various environmental components. The present study was designed to analyze the levels of organochlorine pesticide residu...The ill effects of green revolution include residues of extensively used chemical pesticides in various environmental components. The present study was designed to analyze the levels of organochlorine pesticide residues along with chemical composition in buffalo milk samples collected from different localities of Delhi. Milk monitoring can yield information about the kinds and quantities of pesticides in the environment as well as in our daily diet. In this study, the residue of three different organochlorine pesticides, namely Hexachlorocyclohexane (HCH), Dichlorodiphenyltrichloroethane (DDT) and Endosulfan have been reported. Residues of Lindane exceeded the Maximum Residual Limit values in 50% of the samples is a cause of serious concern. The p,p’-DDT was detected in 70% of the samples with p,p’-DDE (dichlorodiphenyldichloroethylene) in 80% of the milk samples of different parts of Delhi state. DDD (Dichlorodiphenyldichloroethane) another metabolite of p,p’-DDT was detected in 65% of the milk samples. The analysis indicates that DDT is the major contaminants in different parts of Delhi state. α and β endosulfan were detected in 35% and 40% of the samples analyzed. The statistical correlation shows no significant correlation between chemical compositions of the samples. The presence of multiple chemicals in virtually all samples of buffalo milk raises new questions about the possible toxicological impacts of chemical mixtures on an infant’s developing nervous and immune systems and reproductive organs.展开更多
A total of 5852 records concerning daily milk yield, content of protein and fat, and somatic cell count was recorded in two farms (organic and conventional). Somatic cell count was log10- transformed before analysis b...A total of 5852 records concerning daily milk yield, content of protein and fat, and somatic cell count was recorded in two farms (organic and conventional). Somatic cell count was log10- transformed before analysis because was not normally distributed. The mixed model methodology was used according to a repeated-measures scheme, as the restricted maximum likelihood method to estimate lactation curves at different season of calving and at different calving order. Calving order affected milk yield only in the first three calving order with the lowest production levels in the first six months of the year for both farm;no effect could be observed on fat and protein contents. The organic farm shows the minor milk production (7.77 vs8.10 kg/day/head organic and traditional respectively), but good qualitative characteristics of milk, and minor content of Somatic Cell Count. The milk production is major, in traditional farm, in summer respect the winter (10.4 vs6.34 kgof milk/day/ head). In biological farm no difference between seasons were observed. Peak milk yield occurs, for both farms, at around the 6th - 7th wk of lactation.展开更多
The trial was conducted on thirty buffalo cows subdivided in three groups. In the immediate pre-calving, 2.5 ml of Echinacea purpurea were orally administered to the first group (A) and, after calving, Nux vomica, Che...The trial was conducted on thirty buffalo cows subdivided in three groups. In the immediate pre-calving, 2.5 ml of Echinacea purpurea were orally administered to the first group (A) and, after calving, Nux vomica, Chelidonium and Lycopodium were administered at 7 days intervals. The second group (B) was only subjected to the post-calving treatment, while the third group (C) represented the control group. Immediately after calving two groups of calves were formed. The first group received 5 granules of Pyrogenium in the first days of life and then for 10 days the 0.5 ml of E. purpurea. The second group did not receive any remedy. Before the calving, in conventional farm twenty buffalo cows (D group) were vaccinated. Remedies administration did not affect milk protein content, milk lipid contents and the achievement of the lactation peak. Blood samples showed that total protein, albumin and globulin levels were lower in group A, compared to groups B and C. Cholesterol and urea were lower in the treated groups than in the control. We therefore conclude that the use of homeopathic remedies are a chance to improve animal welfare and their productive characteristics.展开更多
建立了液相色谱-串联四极杆质谱(Liquid chromatography tandem four stage mass spectrometry,HPLC-MS/MS)检测信阳水牛乳中唾液酸(Sialic acid,SA)含量的分析方法。采用电喷雾离子源,在负离子采集模式下,利用多重反应监测(MRM)唾液酸...建立了液相色谱-串联四极杆质谱(Liquid chromatography tandem four stage mass spectrometry,HPLC-MS/MS)检测信阳水牛乳中唾液酸(Sialic acid,SA)含量的分析方法。采用电喷雾离子源,在负离子采集模式下,利用多重反应监测(MRM)唾液酸离子对:m/z=308/87,170,定量离子对:m/z=308/87。结果表明,唾液酸含量为0.05~0.50μg/g范围内,线性关系良好(R~2=0.999 9),平均回收率为95.1%, RSD为2.6%,检出限、定量限分别为0.05,0.2μg/g,该方法操作简单,有较好准确性和重现性、高灵敏度,可广泛应用于牛乳中唾液酸(SA)的分析检测,为信阳水牛乳及乳制品的开发探索提供更多可能性。展开更多
利用串联质谱标签(Tandem Mass Tag,TMT)标记定量蛋白质组学技术对水牛初乳和常乳乳清蛋白进行蛋白质组分析,筛选得到具有相关免疫功能的差异蛋白质49种。对这些免疫性差异蛋白进行生物信息学分析发现,其主要参与的生物过程为免疫系统...利用串联质谱标签(Tandem Mass Tag,TMT)标记定量蛋白质组学技术对水牛初乳和常乳乳清蛋白进行蛋白质组分析,筛选得到具有相关免疫功能的差异蛋白质49种。对这些免疫性差异蛋白进行生物信息学分析发现,其主要参与的生物过程为免疫系统过程、防御反应、应激反应;主要参与的细胞组分为胞外区域、血液微粒;主要参与的分子功能为结合受体;主要参与的代谢通路为补体和凝血级联、产生IgA的肠道免疫、造血细胞系和NF kB信号通路等免疫和炎症相关通路。通过蛋白质互作网络分析,找到一些具有高连接度的免疫蛋白因子。本研究为开发增强免疫活性的高端水牛乳制品提供了一定参考。展开更多
文摘The ill effects of green revolution include residues of extensively used chemical pesticides in various environmental components. The present study was designed to analyze the levels of organochlorine pesticide residues along with chemical composition in buffalo milk samples collected from different localities of Delhi. Milk monitoring can yield information about the kinds and quantities of pesticides in the environment as well as in our daily diet. In this study, the residue of three different organochlorine pesticides, namely Hexachlorocyclohexane (HCH), Dichlorodiphenyltrichloroethane (DDT) and Endosulfan have been reported. Residues of Lindane exceeded the Maximum Residual Limit values in 50% of the samples is a cause of serious concern. The p,p’-DDT was detected in 70% of the samples with p,p’-DDE (dichlorodiphenyldichloroethylene) in 80% of the milk samples of different parts of Delhi state. DDD (Dichlorodiphenyldichloroethane) another metabolite of p,p’-DDT was detected in 65% of the milk samples. The analysis indicates that DDT is the major contaminants in different parts of Delhi state. α and β endosulfan were detected in 35% and 40% of the samples analyzed. The statistical correlation shows no significant correlation between chemical compositions of the samples. The presence of multiple chemicals in virtually all samples of buffalo milk raises new questions about the possible toxicological impacts of chemical mixtures on an infant’s developing nervous and immune systems and reproductive organs.
文摘A total of 5852 records concerning daily milk yield, content of protein and fat, and somatic cell count was recorded in two farms (organic and conventional). Somatic cell count was log10- transformed before analysis because was not normally distributed. The mixed model methodology was used according to a repeated-measures scheme, as the restricted maximum likelihood method to estimate lactation curves at different season of calving and at different calving order. Calving order affected milk yield only in the first three calving order with the lowest production levels in the first six months of the year for both farm;no effect could be observed on fat and protein contents. The organic farm shows the minor milk production (7.77 vs8.10 kg/day/head organic and traditional respectively), but good qualitative characteristics of milk, and minor content of Somatic Cell Count. The milk production is major, in traditional farm, in summer respect the winter (10.4 vs6.34 kgof milk/day/ head). In biological farm no difference between seasons were observed. Peak milk yield occurs, for both farms, at around the 6th - 7th wk of lactation.
文摘The trial was conducted on thirty buffalo cows subdivided in three groups. In the immediate pre-calving, 2.5 ml of Echinacea purpurea were orally administered to the first group (A) and, after calving, Nux vomica, Chelidonium and Lycopodium were administered at 7 days intervals. The second group (B) was only subjected to the post-calving treatment, while the third group (C) represented the control group. Immediately after calving two groups of calves were formed. The first group received 5 granules of Pyrogenium in the first days of life and then for 10 days the 0.5 ml of E. purpurea. The second group did not receive any remedy. Before the calving, in conventional farm twenty buffalo cows (D group) were vaccinated. Remedies administration did not affect milk protein content, milk lipid contents and the achievement of the lactation peak. Blood samples showed that total protein, albumin and globulin levels were lower in group A, compared to groups B and C. Cholesterol and urea were lower in the treated groups than in the control. We therefore conclude that the use of homeopathic remedies are a chance to improve animal welfare and their productive characteristics.
文摘建立了液相色谱-串联四极杆质谱(Liquid chromatography tandem four stage mass spectrometry,HPLC-MS/MS)检测信阳水牛乳中唾液酸(Sialic acid,SA)含量的分析方法。采用电喷雾离子源,在负离子采集模式下,利用多重反应监测(MRM)唾液酸离子对:m/z=308/87,170,定量离子对:m/z=308/87。结果表明,唾液酸含量为0.05~0.50μg/g范围内,线性关系良好(R~2=0.999 9),平均回收率为95.1%, RSD为2.6%,检出限、定量限分别为0.05,0.2μg/g,该方法操作简单,有较好准确性和重现性、高灵敏度,可广泛应用于牛乳中唾液酸(SA)的分析检测,为信阳水牛乳及乳制品的开发探索提供更多可能性。
文摘利用串联质谱标签(Tandem Mass Tag,TMT)标记定量蛋白质组学技术对水牛初乳和常乳乳清蛋白进行蛋白质组分析,筛选得到具有相关免疫功能的差异蛋白质49种。对这些免疫性差异蛋白进行生物信息学分析发现,其主要参与的生物过程为免疫系统过程、防御反应、应激反应;主要参与的细胞组分为胞外区域、血液微粒;主要参与的分子功能为结合受体;主要参与的代谢通路为补体和凝血级联、产生IgA的肠道免疫、造血细胞系和NF kB信号通路等免疫和炎症相关通路。通过蛋白质互作网络分析,找到一些具有高连接度的免疫蛋白因子。本研究为开发增强免疫活性的高端水牛乳制品提供了一定参考。
