Citrus yellow vein clearing virus(CYVCV)is a new citrus virus that has become an important factor restricting the development of China’s citrus industry,and the CYVCV coat protein(CP)is associated with viral pathogen...Citrus yellow vein clearing virus(CYVCV)is a new citrus virus that has become an important factor restricting the development of China’s citrus industry,and the CYVCV coat protein(CP)is associated with viral pathogenicity.In this study,the Eureka lemon zinc finger protein(ZFP)ClDOF3.4 was shown to interact with CYVCV CP in vivo and in vitro.Transient expression of ClDOF3.4 in Eureka lemon induced the expression of salicylic acid(SA)-related and hypersensitive response marker genes,and triggered a reactive oxygen species burst,ion leakage necrosis,and the accumulation of free SA.Furthermore,the CYVCV titer in ClDOF3.4 transgenic Eureka lemon plants was approximately 69.4%that in control plants 6 mon after inoculation,with only mild leaf chlorotic spots observed in those transgenic plants.Taken together,the results indicate that ClDOF3.4 not only interacts with CP but also induces an immune response in Eureka lemon by inducing the SA pathways.This is the first report that ZFP is involved in the immune response of a citrus viral disease,which provides a basis for further study of the molecular mechanism of CYVCV infection.展开更多
In this work,Digital Twins based on Neural Networks for the steady state production of styrene were generated.Thus,both the Aspen Technology AI Model Builder(alternative 1)and a homemade MS Excel VBA code connected to...In this work,Digital Twins based on Neural Networks for the steady state production of styrene were generated.Thus,both the Aspen Technology AI Model Builder(alternative 1)and a homemade MS Excel VBA code connected to Aspen HYSYS and Aspen Plus(alternative 2)were used with this same aim.The raw data used for generating the Digital Twins were obtained from process simulations using Aspen HYSYS and/or Aspen Plus,which were connected through a recycle-like stream via automation for solving the entire simulation flowsheet.Aspen HYSYS was used for solving the pre-heating,reaction,and stabilization sections of the process whereas Aspen Plus ensured the computing of the separation and purification columns.Both alternatives led to an excellent prediction showing the capability of creating Digital Twins from and for process simulation.展开更多
基金supported by the China Agriculture Research System of MOF and MARA(CARS26-05B)the Innovation Research 2035 Pilot Plan of Southwest University,China(SWU-XDPY22002)+1 种基金the Guangxi Science and Technology Planed Project,China(Gui Ke AD23026090)the Guangxi Natural Science Foundation,China(2023GXNSFBA026285).
文摘Citrus yellow vein clearing virus(CYVCV)is a new citrus virus that has become an important factor restricting the development of China’s citrus industry,and the CYVCV coat protein(CP)is associated with viral pathogenicity.In this study,the Eureka lemon zinc finger protein(ZFP)ClDOF3.4 was shown to interact with CYVCV CP in vivo and in vitro.Transient expression of ClDOF3.4 in Eureka lemon induced the expression of salicylic acid(SA)-related and hypersensitive response marker genes,and triggered a reactive oxygen species burst,ion leakage necrosis,and the accumulation of free SA.Furthermore,the CYVCV titer in ClDOF3.4 transgenic Eureka lemon plants was approximately 69.4%that in control plants 6 mon after inoculation,with only mild leaf chlorotic spots observed in those transgenic plants.Taken together,the results indicate that ClDOF3.4 not only interacts with CP but also induces an immune response in Eureka lemon by inducing the SA pathways.This is the first report that ZFP is involved in the immune response of a citrus viral disease,which provides a basis for further study of the molecular mechanism of CYVCV infection.
基金V.R.F.thanks to the Aspen Technology Inc.the possibility to participate in the training course“EHM 101:Introduction to Aspen Hybrid Models for Engineering”,where,during the trial time available for AIMB he carried out the case presented in the current paper.
文摘In this work,Digital Twins based on Neural Networks for the steady state production of styrene were generated.Thus,both the Aspen Technology AI Model Builder(alternative 1)and a homemade MS Excel VBA code connected to Aspen HYSYS and Aspen Plus(alternative 2)were used with this same aim.The raw data used for generating the Digital Twins were obtained from process simulations using Aspen HYSYS and/or Aspen Plus,which were connected through a recycle-like stream via automation for solving the entire simulation flowsheet.Aspen HYSYS was used for solving the pre-heating,reaction,and stabilization sections of the process whereas Aspen Plus ensured the computing of the separation and purification columns.Both alternatives led to an excellent prediction showing the capability of creating Digital Twins from and for process simulation.
