Objective: To confirm whetherBungarus multicinctus crude venom induces the apoptosis of K562 tumor cells and to find out the components inducing apoptosis of K562 cells from the crude venom. Methods: the crude venom s...Objective: To confirm whetherBungarus multicinctus crude venom induces the apoptosis of K562 tumor cells and to find out the components inducing apoptosis of K562 cells from the crude venom. Methods: the crude venom separated and purified by cation exchange chromatography, and the effect of venoms on K562 was studied by MTT method and flow cytometry. Results: The crude venom began to kill K562 cells at than 8×l03ng/ml (the survival rate was 82.5%) concentration and the effect was more significant in 24 h when administrating 8×l05ng/ml (the survival rate was 29.4%) crude venom. Apoptotic bodies were observed in the K562 tumor cells by fluorescent microscopy after administration of 5 μg/ml cycloheximide (CHX) or the peak VI solution at about 8×105 ng/ml. The same results were detected by the flow cytometry. A sub-Gi peak appeared after administration of CHX or the sixth peak solution. Conclusion: The authors found that the venom can kill K562 tumor cells in time- and dose-dependent manner. However, the killing effect of the venom is not apoptosis. What’s more, the peak VI solution, a component of the crude venom can induce the apoptosis of K562 tumor cells.展开更多
Based on molecular phylogeny of the Chinese white cross-band krait, Bungarus multicinctus, several individuals from southwestern and southern China were found to be nested within putative species B. candidus（Linnaeus...Based on molecular phylogeny of the Chinese white cross-band krait, Bungarus multicinctus, several individuals from southwestern and southern China were found to be nested within putative species B. candidus（Linnaeus, 1758） with high support values. Morphological examination also indicated these specimens were identical to B. candidus. We concluded that the Chinese white cross-band krait, previously identified as B. multicinctus in southwest and south China, should be recognized as B. candidus, a new record in China, and B. m. wanghaoti should be invalid. B. multicinctus is recurrently limited to southern China within China.展开更多
基金This work was supported by the National Natural Science Foundation of China(No. 39570238)
文摘Objective: To confirm whetherBungarus multicinctus crude venom induces the apoptosis of K562 tumor cells and to find out the components inducing apoptosis of K562 cells from the crude venom. Methods: the crude venom separated and purified by cation exchange chromatography, and the effect of venoms on K562 was studied by MTT method and flow cytometry. Results: The crude venom began to kill K562 cells at than 8×l03ng/ml (the survival rate was 82.5%) concentration and the effect was more significant in 24 h when administrating 8×l05ng/ml (the survival rate was 29.4%) crude venom. Apoptotic bodies were observed in the K562 tumor cells by fluorescent microscopy after administration of 5 μg/ml cycloheximide (CHX) or the peak VI solution at about 8×105 ng/ml. The same results were detected by the flow cytometry. A sub-Gi peak appeared after administration of CHX or the sixth peak solution. Conclusion: The authors found that the venom can kill K562 tumor cells in time- and dose-dependent manner. However, the killing effect of the venom is not apoptosis. What’s more, the peak VI solution, a component of the crude venom can induce the apoptosis of K562 tumor cells.
基金supported by the National Natural Science Foundation of China(31071892,31372152)Department of Education of Sichuan Province(13TD0027)to PGSoutheast Asia Biodiversity Research Institute,Chinese Academy of Sciences(Y4ZK111B01)to JC
文摘Based on molecular phylogeny of the Chinese white cross-band krait, Bungarus multicinctus, several individuals from southwestern and southern China were found to be nested within putative species B. candidus（Linnaeus, 1758） with high support values. Morphological examination also indicated these specimens were identical to B. candidus. We concluded that the Chinese white cross-band krait, previously identified as B. multicinctus in southwest and south China, should be recognized as B. candidus, a new record in China, and B. m. wanghaoti should be invalid. B. multicinctus is recurrently limited to southern China within China.
