Phytochemical and biological studies of Butia capitata Becc.leaves cultivated in Egypt

Objective:To study the antioxidant and anti-inflammatory activity of Butia capitata(B.capitata)leaf extracts along with phytochemical analysis of the proposed bioactive constituents.Methods:Different successive extracts of B.capitata Becc.leaves were prepared with selective organic solvents and screened for their anti-inflammatory activities in tested animals and invitro antioxidant effect.An extensive phytochemical investigation of the bioactive extracts through paper chromatography,thin layer chromatography,column chromatography,gasliquid chromatography(GLC),high pressure liquid chromatography and spectral analysis.GCMass,ultraviolet,hydrogen and carbon nuclear magnetic resonance,electron ionization-mass spectrometry,heteronuclear multiple bond correlation and heteronuclear multiple quantum correlation were carried out Results:Results showed that different extracts possess promising antioxidant effect and significant anti-inflammatory activity with variable degrees.The results of the phytochemical investigation of the bioactive extracts revealed the presence of volatile substances,lipoidal matter,α-tocopherol,free sugars,polysaccharides and flavonoidal compounds.Conclusions:B.capitata leaf extracts were shown to possess variable antioxidant effect,the most promising was methanol extract Both polar and non polar extracts were proved to have anti-inflammatory activity,the non polar extract was superior in this respect The bioactivity of the extracts was ascribed to the presence of flavonoids,sterols andα-tocopherol.

低温胁迫下弓葵幼苗膜脂过氧化及保护酶活性的变化

低温胁迫下弓葵 (ButiacapitataBecc)幼苗叶片的MDA含量逐渐增加 ,膜脂过氧化作用增强。- 8℃条件下的膜脂过氧化作用明显强于 2℃。细胞膜透性在 2℃条件下变化不大 ,- 8℃时则随低温胁迫时间延长而急剧上升 ,细胞膜受到伤害。 - 8℃胁迫下细胞保护酶SOD、POD和CAT活性短期 (6h)内升高 ,然后下降 ,2 4h以后 3种保护酶受到低温胁迫的严重抑制。在 2℃胁迫下 ,SOD活性在 6h内变化不大 ,随后下降 ;CAT活性变化趋势与 - 8℃时相似 ,但变化幅度较小 ;POD虽也呈现先升后降的趋势 ,但降幅明显小于升幅 ,至 4 8h时POD活性仍维持较高水平。

布迪椰子不同器官热值的季节变化研究

对布迪椰子的幼叶、成熟叶、叶柄和根在不同季节的干重热值、去灰分热值和灰分含量进行了研究,结果表明:干重热值四个季节的平均值为成熟叶(20.65kJg-1)>幼叶(19.84kJg-1)>根(19.55kJg-1)>叶柄(18.77kJg-1),秋季的干重热值明显高于其它三个季节的干重热值,冬季的干重热值最低,去灰分热值与干重热值的变化趋势基本相同。灰分含量四个季节的平均值为根(5.14%)>叶柄(4.33%)>幼叶(4.21%)>成熟叶(3.97%)。成熟叶的灰分含量一直维持在比较低的水平,而幼叶的在秋季明显下降,在冬季明显上升,幼叶灰分含量的季节变化趋势与成熟叶的相同,叶柄灰分含量在冬季明显低于根部。布迪椰子这种不同器官在不同季节的热值和灰分的变化规律显示其具有较强的耐寒适应性。

布迪椰子种子成熟胚诱导愈伤组织的研究

在含有0．5 mg/L NAA＋0．5 mg/L 6-BA＋60．0 g/L蔗糖＋6．0 g/L琼脂的MS培养基上分别添加6种不同浓度的2，4-D，对3种不同树龄布迪椰子种子的成熟胚进行愈伤组织诱导。结果表明：以6～7 a生布迪椰子种子成熟胚的诱导愈伤组织效果最好，添加2，4-D则以浓度为0．5 mg/L的效果最好。

布迪椰子的主要特性及其栽培技术

布迪椰子可耐-22℃，是已知棕榈科植物中耐寒力较强的树种之一，其气候适应范围广，是一种极受欢迎的园林景观树种。布迪椰子采用种子繁殖，当小苗长出2～3叶、侧根生长较为发达时移至营养袋育苗，一年后幼苗长出3～5叶时定植大田，当植株的基部直径达20～25cm、高1．5～2．0m时可用于园林绿化。

布迪椰子耐旱性及其在园林景观中的应用

采用干旱胁迫模拟试验，进行布迪椰子耐旱特性研究，结果表明：不同年龄布迪椰子的耐旱性指标存在明显差异，随布迪椰子叶龄增加，布迪椰子叶4水势下降，蒸腾速率和自然饱和亏含量增加，布迪椰子耐旱增强；布迪椰子水分指标对干旱胁迫反应敏感，随干旱胁迫加剧，布迪椰子叶片水势和蒸腾速率下降，而自然饱和亏增大，以适应胁迫环境，使布迪椰子表现出较强的抗旱性和生命力。根据布迪椰子耐旱特性，在园林景观中主要有丛植、列植和孤植3种应用形式，可广泛应用于园林绿化。