目的:研究P38丝裂原活化蛋白激酶(P38mitogen-activated protein kinases,P38MAPK)通路在骨形态发生蛋白9(Bonemorphogenetic protein 9,BMP9)诱导C3H10T1/2干细胞向心肌样细胞分化中的作用。方法:免疫印迹检测经pAdEasyBMP9诱导24 h的C...目的:研究P38丝裂原活化蛋白激酶(P38mitogen-activated protein kinases,P38MAPK)通路在骨形态发生蛋白9(Bonemorphogenetic protein 9,BMP9)诱导C3H10T1/2干细胞向心肌样细胞分化中的作用。方法:免疫印迹检测经pAdEasyBMP9诱导24 h的C3H10T1/2干细胞磷酸化P38MAPK和P38MAPK表达水平,免疫荧光定位磷酸化P38MAPK;SB203580抑制C3H10T1/2干细胞磷酸化P38MAPK活性,pAdEasyBMP9诱导21 d时免疫印迹检测心肌特异性蛋白CX43、cTnT表达,免疫荧光检测心肌特异性蛋白cTnT、α-MHC表达,RT-qPCR检测心肌特异性基因GATA4、MEF2C表达。结果:pAdEasyBMP9促进磷酸化P38MAPK表达增高,却不影响P38MAPK表达水平;磷酸化P38MAPK有表达于胞浆和胞核、仅表达于胞核的不同状态。SB203580可显著性地抑制由pAdEasyBMP9诱导的C3H10T1/2干细胞CX43、cTnT、α-MHC、GATA4、MEF2C表达。结论:pAdEasyBMP9诱导C3H10T1/2干细胞能激活P38MAPK通路,并促进其向心肌样细胞分化。展开更多
Converting hydrocarbons into aldehydes in a green and environmentally benign way is of great signif-icance in fine chemistry.In this work,all-inorganic Cs_(3)Bi_(2)B_(9) perovskite nanoparticles were uniformly loaded ...Converting hydrocarbons into aldehydes in a green and environmentally benign way is of great signif-icance in fine chemistry.In this work,all-inorganic Cs_(3)Bi_(2)B_(9) perovskite nanoparticles were uniformly loaded on BiOBr nanosheets via an in-situ growth method,which can selectivity photoactivate aromatic C(sp3)-H bond of toluene to generate benzaldehyde.According to the in-situ X-ray photoelectron spec-troscopy characterization,the photogenerated electrons of BiOBr transfer to Cs_(3)Bi_(2)B_(9) enforced by the in-ternal electric field under light irradiation,resulting in S-scheme heterojunction.Furthermore,theoretical calculations indicate that toluene molecules are inclined to adsorb on the BiOBr surface,subsequently in-volving the oxidation reaction to generate benzyl radical(PhCH_(2)·)by using the energetic holes of BiOBr,while the remaining photoinduced electrons in the conduction band(CB)of Cs_(3)Bi_(2)B_(9) with powerful reduction ability reduce O2 into·O_(2)^(-),which is the vital oxidative active species working on toluene selective oxidation process.Such an unexceptionable charge carrier utilization mode and tendentious ad-sorption behavior of reactants contribute to the optimized Cs_(3)Bi_(2)B_(9)/BiOBr heterojunction with excellent photocatalytic performance,achieving a maximum of 22.5%toluene conversion and 96.2%selectivity to-wards benzaldehyde formation.This work provides a rational photocatalyst heterojunction construction protocol for the selective oxidation of saturated aromatic C-H bonds.展开更多
Diamond like carbon films, prepared by RF glow discharge on glass substrates, were irradiated by γ rays. The as deposited and irradiated films were characterized by Raman spectroscopy, electrical resistivity, a...Diamond like carbon films, prepared by RF glow discharge on glass substrates, were irradiated by γ rays. The as deposited and irradiated films were characterized by Raman spectroscopy, electrical resistivity, and infrared transmittance. It is shown that the irradiation of the γ rays can lead to the breaking of SP 3 C H and SP 2 C H bonds, slight increasing of SP 3 C C bonds, and induced hydrogen recombination with H 2 molecules, subsequently diffusing to the surface of the films. When the γ rays irradiation dose reached 10×10 4 Gy, the numbers of SP 3 C H bonds was decreased by about 50%, the resistivity of irradiated DLC films was increased, and the diamond like character of the films became more obvious. The structure of DLC films was modified when irradiated by γ rays. The irradiation mechanisms are briefly discussed.展开更多
文摘目的:研究P38丝裂原活化蛋白激酶(P38mitogen-activated protein kinases,P38MAPK)通路在骨形态发生蛋白9(Bonemorphogenetic protein 9,BMP9)诱导C3H10T1/2干细胞向心肌样细胞分化中的作用。方法:免疫印迹检测经pAdEasyBMP9诱导24 h的C3H10T1/2干细胞磷酸化P38MAPK和P38MAPK表达水平,免疫荧光定位磷酸化P38MAPK;SB203580抑制C3H10T1/2干细胞磷酸化P38MAPK活性,pAdEasyBMP9诱导21 d时免疫印迹检测心肌特异性蛋白CX43、cTnT表达,免疫荧光检测心肌特异性蛋白cTnT、α-MHC表达,RT-qPCR检测心肌特异性基因GATA4、MEF2C表达。结果:pAdEasyBMP9促进磷酸化P38MAPK表达增高,却不影响P38MAPK表达水平;磷酸化P38MAPK有表达于胞浆和胞核、仅表达于胞核的不同状态。SB203580可显著性地抑制由pAdEasyBMP9诱导的C3H10T1/2干细胞CX43、cTnT、α-MHC、GATA4、MEF2C表达。结论:pAdEasyBMP9诱导C3H10T1/2干细胞能激活P38MAPK通路,并促进其向心肌样细胞分化。
基金National Natural Science Foundation of China(Nos.21905209,52171145,and 51973078)Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2020D01B25).
文摘Converting hydrocarbons into aldehydes in a green and environmentally benign way is of great signif-icance in fine chemistry.In this work,all-inorganic Cs_(3)Bi_(2)B_(9) perovskite nanoparticles were uniformly loaded on BiOBr nanosheets via an in-situ growth method,which can selectivity photoactivate aromatic C(sp3)-H bond of toluene to generate benzaldehyde.According to the in-situ X-ray photoelectron spec-troscopy characterization,the photogenerated electrons of BiOBr transfer to Cs_(3)Bi_(2)B_(9) enforced by the in-ternal electric field under light irradiation,resulting in S-scheme heterojunction.Furthermore,theoretical calculations indicate that toluene molecules are inclined to adsorb on the BiOBr surface,subsequently in-volving the oxidation reaction to generate benzyl radical(PhCH_(2)·)by using the energetic holes of BiOBr,while the remaining photoinduced electrons in the conduction band(CB)of Cs_(3)Bi_(2)B_(9) with powerful reduction ability reduce O2 into·O_(2)^(-),which is the vital oxidative active species working on toluene selective oxidation process.Such an unexceptionable charge carrier utilization mode and tendentious ad-sorption behavior of reactants contribute to the optimized Cs_(3)Bi_(2)B_(9)/BiOBr heterojunction with excellent photocatalytic performance,achieving a maximum of 22.5%toluene conversion and 96.2%selectivity to-wards benzaldehyde formation.This work provides a rational photocatalyst heterojunction construction protocol for the selective oxidation of saturated aromatic C-H bonds.
文摘Diamond like carbon films, prepared by RF glow discharge on glass substrates, were irradiated by γ rays. The as deposited and irradiated films were characterized by Raman spectroscopy, electrical resistivity, and infrared transmittance. It is shown that the irradiation of the γ rays can lead to the breaking of SP 3 C H and SP 2 C H bonds, slight increasing of SP 3 C C bonds, and induced hydrogen recombination with H 2 molecules, subsequently diffusing to the surface of the films. When the γ rays irradiation dose reached 10×10 4 Gy, the numbers of SP 3 C H bonds was decreased by about 50%, the resistivity of irradiated DLC films was increased, and the diamond like character of the films became more obvious. The structure of DLC films was modified when irradiated by γ rays. The irradiation mechanisms are briefly discussed.
