Idioms are the marrow in a language, in which it is easy to find a lot of cultural information. English and Chinese people are different in many aspects, such as geography and history, customs, religion and belief, va...Idioms are the marrow in a language, in which it is easy to find a lot of cultural information. English and Chinese people are different in many aspects, such as geography and history, customs, religion and belief, value and concept, etc.It is a very important task in cross-culture communication that the understanding and translation of English and Chinese idioms. This paper mainly analyzes the implicit information lied in English and Chinese idioms.Further, it will figure out the cultural factors influencing English and Chinese people's cognition.Finally, it reveals the cognitive mechanism in different cultures so as to accelerate the cross-culture communication.展开更多
AIM： To construct the plasmid pcHEV23 containing fragments of HEV ORF2 and ORF3 chimeric gene and to assess its ability to elicit specific immunologic response in mice. METHODS： The gene encoding the structural prot...AIM： To construct the plasmid pcHEV23 containing fragments of HEV ORF2 and ORF3 chimeric gene and to assess its ability to elicit specific immunologic response in mice. METHODS： The gene encoding the structural protein of HEV ORF2 fragment and full-length ORF3 was amplified by PCR. The PCR products were cloned into an eucaryotic expression plasmid pcDNA3. The resulting plasmid pcHEV23 was used as a DNA vaccine to inoculate BALB/c mice intramuscularly thrice at a dose of 100 or 200 ug. Mice injected with empty pcDNA3 DNA or saline served as control and then specific immune responses in the mice were detected. RESULTS： After 2-3 times of inoculation, all mice injected with pcHEV23 had anti-HEV IgG seroconversion and specific T lymphocyte proliferation. The lymphocyte stimulation index in the group immunized with pcHEV23 （3.1＋0.49） was higher than that in the control group （0.787±0.12, P〈0.01）. None in the control group had a detectable level of anti-HEV IgG. CONCLUSION： DNA vaccine containing HEV ORF2 and ORF3 chimeric gene can successfully induce specific humoral and cellular immune response in mice.展开更多
文摘Idioms are the marrow in a language, in which it is easy to find a lot of cultural information. English and Chinese people are different in many aspects, such as geography and history, customs, religion and belief, value and concept, etc.It is a very important task in cross-culture communication that the understanding and translation of English and Chinese idioms. This paper mainly analyzes the implicit information lied in English and Chinese idioms.Further, it will figure out the cultural factors influencing English and Chinese people's cognition.Finally, it reveals the cognitive mechanism in different cultures so as to accelerate the cross-culture communication.
基金Supported by the Grants from the Natural Science Foundation of Zhejiang Province, No. RC01054, Science Technology Department of Zhejiang Province, No. F11023 and Key Project of Health Bureau of Zhejiang Province
文摘AIM： To construct the plasmid pcHEV23 containing fragments of HEV ORF2 and ORF3 chimeric gene and to assess its ability to elicit specific immunologic response in mice. METHODS： The gene encoding the structural protein of HEV ORF2 fragment and full-length ORF3 was amplified by PCR. The PCR products were cloned into an eucaryotic expression plasmid pcDNA3. The resulting plasmid pcHEV23 was used as a DNA vaccine to inoculate BALB/c mice intramuscularly thrice at a dose of 100 or 200 ug. Mice injected with empty pcDNA3 DNA or saline served as control and then specific immune responses in the mice were detected. RESULTS： After 2-3 times of inoculation, all mice injected with pcHEV23 had anti-HEV IgG seroconversion and specific T lymphocyte proliferation. The lymphocyte stimulation index in the group immunized with pcHEV23 （3.1＋0.49） was higher than that in the control group （0.787±0.12, P〈0.01）. None in the control group had a detectable level of anti-HEV IgG. CONCLUSION： DNA vaccine containing HEV ORF2 and ORF3 chimeric gene can successfully induce specific humoral and cellular immune response in mice.
