【目的】研究bla_(CMY-2)阳性禽源奇异变形杆菌的多重耐药特征,并分析菌株CY32全基因组序列结构。【方法】对5株bla_(CMY-2)阳性禽源奇异变形杆菌进行氟苯尼考和质粒介导氟喹诺酮类耐药基因检测、接合试验和bla_(CMY-2)基因的Southern...【目的】研究bla_(CMY-2)阳性禽源奇异变形杆菌的多重耐药特征,并分析菌株CY32全基因组序列结构。【方法】对5株bla_(CMY-2)阳性禽源奇异变形杆菌进行氟苯尼考和质粒介导氟喹诺酮类耐药基因检测、接合试验和bla_(CMY-2)基因的Southern杂交定位,对其中一株菌CY32进行全基因测序和生物信息学分析。【结果】5株奇异变形杆菌携带的bla_(CMY-2)位于染色体,其中,菌株CY12、CY32、S31和S52携带floR,菌株CY12和CY32携带qnrD。CY32的染色体同时含有SXT/R391型整合性接合元件(integrative and conjugative elements,ICEs)(ICEPmiJpn1)和PmGRI1共2种耐药基因岛。ICEs的可变区包含2个串联的复合型转座子(IS10构成),其中一个复合型转座子携带bla_(CMY-2);CY32的PmGRI1耐药岛含有12个耐药基因。与其他奇异变形杆菌携带的PmGRI1相比,多重耐药区差异最大的区域位于Tn21转座子。此外,CY32包含2个质粒,包括携带floR的IncQ质粒和携带qnrD的非接合质粒。奇异变形杆菌CY32携带15个耐药基因,呈现多重耐药的特性。【结论】奇异变形杆菌经基因岛和质粒获得多重耐药,使治疗奇异变形杆菌感染变得更加困难,应加强对动物源奇异变形杆菌耐药性监测。展开更多
[ Objective] The difference of mineral element content in healthy bark of P. armandii and bark affected by C. nbicola were compared, which would provide theoretical foundation for exploring new ways to control the dis...[ Objective] The difference of mineral element content in healthy bark of P. armandii and bark affected by C. nbicola were compared, which would provide theoretical foundation for exploring new ways to control the disease by artificial water and fertilizer. [ Method] Inductively Coupled Plasma Optical Emission Spectrometer（ ICP-OES）was adopted to measure the variation of mineral elements content in bark after P. armandii was affected by C. nbicola. [Result] 45 mineral elements in the healthy bark and 46 mineral elements in affected bark caused by C. ribicola were detected. These mineral elements contained 14 kinds of mineral elements which were necessary for plant growth and 31 kinds of other mineral elements （32 kinds after affected disease）. The total content of mineral elements in affected bark of P. armandii decreased for 15.77%, in which necessary elements for plant growth decreased for 15.54% and other elements decreased for 17.07%. The content of Ca decreased the most, decrease rate was about 75%. The order of content was also changed, they were Na 〉 Ca 〉 B 〉 K 〉 Si 〉 Mg 〉 S 〉 P in healthy bark, and Na 〉 B 〉 K 〉 Ca 〉 Mg 〉 P 〉 Si 〉 S in affected bark. [ Conclusion] The results laid foundation for controlling C. ribicola through application of mineral elements.展开更多
C^1 natural element method （C^1 NEM） is applied to strain gradient linear elasticity, and size effects on mi crostructures are analyzed. The shape functions in C^1 NEM are built upon the natural neighbor interpolati...C^1 natural element method （C^1 NEM） is applied to strain gradient linear elasticity, and size effects on mi crostructures are analyzed. The shape functions in C^1 NEM are built upon the natural neighbor interpolation （NNI）, with interpolation realized to nodal function and nodal gradient values, so that the essential boundary conditions （EBCs） can be imposed directly in a Galerkin scheme for partial differential equations （PDEs）. In the present paper, C^1 NEM for strain gradient linear elasticity is constructed, and sev- eral typical examples which have analytical solutions are presented to illustrate the effectiveness of the constructed method. In its application to microstructures, the size effects of bending stiffness and stress concentration factor （SCF） are studied for microspeciem and microgripper, respectively. It is observed that the size effects become rather strong when the width of spring for microgripper, the radius of circular perforation and the long axis of elliptical perforation for microspeciem come close to the material characteristic length scales. For the U-shaped notch, the size effects decline obviously with increasing notch radius, and decline mildly with increasing length of notch.展开更多
The effect of converter high pressure combined blowing technology on the damage of MgO-C permeable elements is discussed in the paper.A remnant high pressure gas permeable element is used to analyse the damage mechani...The effect of converter high pressure combined blowing technology on the damage of MgO-C permeable elements is discussed in the paper.A remnant high pressure gas permeable element is used to analyse the damage mechanism of high pressure permeable element.The view that spall caused by high concentrated thermal stress and intensive erosion-wear action cause high pressure gas permeable elements damage mainly and a reasonable measures to increase the service life of MgO-C high pressure permeable elements are also introduced in the paper.展开更多
文摘【目的】研究bla_(CMY-2)阳性禽源奇异变形杆菌的多重耐药特征,并分析菌株CY32全基因组序列结构。【方法】对5株bla_(CMY-2)阳性禽源奇异变形杆菌进行氟苯尼考和质粒介导氟喹诺酮类耐药基因检测、接合试验和bla_(CMY-2)基因的Southern杂交定位,对其中一株菌CY32进行全基因测序和生物信息学分析。【结果】5株奇异变形杆菌携带的bla_(CMY-2)位于染色体,其中,菌株CY12、CY32、S31和S52携带floR,菌株CY12和CY32携带qnrD。CY32的染色体同时含有SXT/R391型整合性接合元件(integrative and conjugative elements,ICEs)(ICEPmiJpn1)和PmGRI1共2种耐药基因岛。ICEs的可变区包含2个串联的复合型转座子(IS10构成),其中一个复合型转座子携带bla_(CMY-2);CY32的PmGRI1耐药岛含有12个耐药基因。与其他奇异变形杆菌携带的PmGRI1相比,多重耐药区差异最大的区域位于Tn21转座子。此外,CY32包含2个质粒,包括携带floR的IncQ质粒和携带qnrD的非接合质粒。奇异变形杆菌CY32携带15个耐药基因,呈现多重耐药的特性。【结论】奇异变形杆菌经基因岛和质粒获得多重耐药,使治疗奇异变形杆菌感染变得更加困难,应加强对动物源奇异变形杆菌耐药性监测。
基金Project(22A0117)supported by Hunan Provincial Department of Education,ChinaProject(AWJ-20-M02)supported by State Key Laboratory of Advanced Welding and Joining,Harbin Institute of Technology,China。
基金Supported by The 10~(th) Five-Years Key Programs for Science and Technology Development of Yunnan Province(2003NG12)~~
文摘[ Objective] The difference of mineral element content in healthy bark of P. armandii and bark affected by C. nbicola were compared, which would provide theoretical foundation for exploring new ways to control the disease by artificial water and fertilizer. [ Method] Inductively Coupled Plasma Optical Emission Spectrometer（ ICP-OES）was adopted to measure the variation of mineral elements content in bark after P. armandii was affected by C. nbicola. [Result] 45 mineral elements in the healthy bark and 46 mineral elements in affected bark caused by C. ribicola were detected. These mineral elements contained 14 kinds of mineral elements which were necessary for plant growth and 31 kinds of other mineral elements （32 kinds after affected disease）. The total content of mineral elements in affected bark of P. armandii decreased for 15.77%, in which necessary elements for plant growth decreased for 15.54% and other elements decreased for 17.07%. The content of Ca decreased the most, decrease rate was about 75%. The order of content was also changed, they were Na 〉 Ca 〉 B 〉 K 〉 Si 〉 Mg 〉 S 〉 P in healthy bark, and Na 〉 B 〉 K 〉 Ca 〉 Mg 〉 P 〉 Si 〉 S in affected bark. [ Conclusion] The results laid foundation for controlling C. ribicola through application of mineral elements.
基金supported by the SDUST Spring Bud (2009AZZ021)Taian Science and Technology Development (20112001)
文摘C^1 natural element method （C^1 NEM） is applied to strain gradient linear elasticity, and size effects on mi crostructures are analyzed. The shape functions in C^1 NEM are built upon the natural neighbor interpolation （NNI）, with interpolation realized to nodal function and nodal gradient values, so that the essential boundary conditions （EBCs） can be imposed directly in a Galerkin scheme for partial differential equations （PDEs）. In the present paper, C^1 NEM for strain gradient linear elasticity is constructed, and sev- eral typical examples which have analytical solutions are presented to illustrate the effectiveness of the constructed method. In its application to microstructures, the size effects of bending stiffness and stress concentration factor （SCF） are studied for microspeciem and microgripper, respectively. It is observed that the size effects become rather strong when the width of spring for microgripper, the radius of circular perforation and the long axis of elliptical perforation for microspeciem come close to the material characteristic length scales. For the U-shaped notch, the size effects decline obviously with increasing notch radius, and decline mildly with increasing length of notch.
文摘The effect of converter high pressure combined blowing technology on the damage of MgO-C permeable elements is discussed in the paper.A remnant high pressure gas permeable element is used to analyse the damage mechanism of high pressure permeable element.The view that spall caused by high concentrated thermal stress and intensive erosion-wear action cause high pressure gas permeable elements damage mainly and a reasonable measures to increase the service life of MgO-C high pressure permeable elements are also introduced in the paper.
