Recurrent hepatitis C virus after transplant and the importance of plasma cells on biopsy

Hepatitis C virus(HCV) is the leading indication for liver transplantation in the United States.It recurs universally after transplant but the rate of fibrosis and the development of graft failure is variable.Different donor and recipient features have been demonstrated to impact fibrosis.Plasma cell hepatitis,a histologic finding,is one feature associated with poor graft and patient outcomes.The pathogenic mechanism resulting in plasma cell hepatitis is poorly understood,with evidence suggesting a role for both the HCV and the immune system.A recent publication described plasma cell hepatitis in a larger context of immune medicated graft dysfunction in transplant recipients receiving interferon based therapy.This manuscript will highlight the topic of plasma cell hepatitis and provide commentary on the lack of recognition,the data regarding pathophysiologic mechanisms and the potential management options.

Preparation and Characterization of High Quality Diamond Films by DC ArcPlasma Jet CVD Method

Under optimal conditions free-standing high quality diamond films were prepared by DC arc plasma jet CVD method at a growth rate of 7-10 Pm/h. Surface and cross section morphologies of the diamond films were observed by SEM. Raman spectrometer wasused to characterize the quality of diamond films. The IR transmittivity measured by IR spectrometer is close to the theoretical value ofabout 71% in the far infrared band. The thermal conductivity measured by photothermal deflection exceeds 18 W/cm' K. <l 10> is thepreferential growth orientation of the films detected by X-ray diffractometer. As s result, the extremely high temperature of DC arc plasma jet can produce supersaturated atomic hydrogen, which played an important role in the process for the deposition of high quality diamond films.

Plasma apo CⅢ Levels in Relation to Inflammatory Traits and Metabolic Syndrome in Patients not Treated with Lipid-lowering Drugs Undergoing Coronary Angiography

Objective Assessment of the comprehensive relationship among apolipoprotein CIII（apoCⅢ） levels, inflammation, and metabolic disorders is rare. Methods A total of 1455 consecutive patients not treated with lipid-lowering drugs and undergoing coronary angiography were enrolled in this cross-sectional study. A mediation analysis was used to detect the underlying role of apoCⅢ in the association of inflammation with metabolic syndrome（MetS）. Results Patients with MetS showed higher levels of apoCⅢ [95.1（73.1-131.4） vs. 81.7（58.6-112.4） μg/mL, P 〈 0.001] and inflammatory markers [high sensitivity C-reactive protein, 1.7（0.8-3.4） vs. 1.1（0.5-2.2） mg/L; white blood cell count,（6.48 ± 1.68） vs.（6.11 ± 1.67） × 10~9/L]. The levels of apoCⅢ and inflammatory markers increased with the number of metabolic risk components（all P 〈 0.001）. Furthermore, apoCⅢ levels were associated with virtually all individual MetS risk factors and inflammatory markers（all P 〈 0.05）. Importantly, the prevalence of MetS in each metabolic disorder rose as apoCⅢ levels increased（all P 〈 0.05）. Mediation analysis showed that apoCⅢ partially mediated the effect of inflammation on MetS independently from triglycerides. Conclusion Plasma apoCⅢ levels were significantly associated with the development and severity of MetS, and a role of apoCⅢ in the effect of inflammation on the development of MetS was identified.

沙库巴曲缬沙坦钠片联合叶酸治疗对H型高血压患者血压、sdLDL-C水平及血浆Hcy的影响

目的 探讨沙库巴曲缬沙坦钠片联合叶酸治疗对H型高血压患者血压、sdLDL-C水平及血浆Hcy的影响。方法 选取2021年10月至2022年9月于淄博市第一医院进行治疗的176例H型高血压患者为受试者,按照随机双盲法将其分为2组。对照组受试者采用沙库巴曲缬沙坦钠片进行治疗;研究组受试者在对照组基础上给予叶酸进行治疗。观察两组受试者临床疗效、血压、血脂代谢指标、血清炎症指标情况。结果 研究组受试者临床疗效总有效率为92.05%远高于对照组,差异有统计学意义(χ^(2)=3.965,P<0.05)。研究组受试者治疗后收缩压,舒张压均低于对照组,差异有统计学意义(t=6.007、4.186,P<0.05);经治疗后,研究组受试者血脂代谢指标TC、TG、HDL-C、LDL-C和sdLDL-C水平均较对照组低,差异有统计学意义,(t=19.705、19.105、13.304、13.466、10.713,P<0.05);两组受试者经治疗后,研究组受试者血清炎症指标IL-6、TNF-α、hs-CRP、Hcy均优于对照组,差异有统计学意义(t=19.705、19.105、13.304、13.466、10.713,P<0.05)。结论 沙库巴曲缬沙坦钠片联合叶酸治疗H型高血压具有较好的临床效果,能有效降低H型高血压患者的血压,改善患者血脂情况,同时降低患者体内的血清炎症指标,具有较高的临床应用价值,值得推广。

低温等离子体处理^(14)C烷烃类化合物的实验研究

压水堆中的^(14)C气态流出物主要以烷烃类化合物的形式存在。本研究以^(14)C烷烃类化合物中占比最大且化学性质最稳定的^(14)CH_(4)作为处理目标,引入低温等离子体技术,对其放电行为和CH_(4)处理性能进行探究。结果表明:在常温常压、输出电压17.89 kV、气体流速0.83 cm/s的最优条件下,等离子体的CH_(4)处理效率可达99.37%,CO_(2)选择性可达46.99%;通过提高输出电压、反应温度以及降低气体流速均能有效提升等离子体的CH_(4)处理性能;除CO_(2)外,等离子体处理CH_(4)过程中伴随产生的副产物有30余种,以有机物为主;等离子体处理CH_(4)的动力学过程符合准一级反应动力学模型,相应的速率常数为1.1048 m^(3)/(kW·h)。以上结果表明,等离子体技术在^(14)C废气处理和监测领域,尤其是^(14)C烷烃类化合物处理方面具有广阔的发展前景。

The Clinic Study on Synchronous Detection of HCV RNA in the Plasma and PBMC of Hepatitis C Patients

In order to increase the positive detection rate of HCV RNA in the patients with chronic hepatitis C , RT PCR was used to synchronously detect HCV RNA in the plasma and peripheral blood mononuclear cells of 583 CHC patients with a continuously elevated level of ALT for more than one year. The results showed that the positive detection rate of HCV RNA in the plasma of the CHC patients was 19.2 %, while 24.5 % in PBMC. It was demonstrated that the positive detection rate for HCV RNA in PBMC was obviously higher than that detected in plasma. To synchronously detect HCV RNA in PBMC by using RT PCR can increase the positive detection rate of HCV RNA in the CHC patients.

Effects of C,N-Codoped on the Corrosion Resistance of TiO_2 Films Prepared by Plasma Surface Alloying and Thermal Oxidation Duplex Process

C,N-codoped TiO 2 films have been deposited onto stainless steel substrates using plasma surface alloying and thermal oxidation duplex process.Composition analysis shows that the films shield the substrates entirely.The TiO 2 films are anatase in structure as characterized by X-ray diffraction.The electrochemical measurements show that the equilibrium corrosion potential positively shifts from-0.275 eV for bare stainless steel to-0.267 eV for C,N-codoped TiO 2 coated stainless steel,and the corrosion current density decreases from 1.3×10-5 A/cm2 to 4.1×10-6 A/cm2.The corrosion resistance obtained by electrochemistry noise also reveals that the C,N-codoped TiO 2 films provide good protection for stainless steel against corrosion in stimulated body fluid.The above results indicate that C,N-codoped TiO 2 films deposited by plasma surface alloying and thermal oxidation duplex process are effective in protecting stainless steel from corrosion.

SiC Formation Through Interface Reaction between C_(60) and Si in Plasma Environment

The formation of SiC through the interface reaction between C60 and Si in a plasmaassisted chemical vapour deposition system （PACVD） is investigated with a C60 film previously deposited on Si wafers. The composition and structure of the deposited samples were characterized by micro-Raman spectroscopy and X-ray diffraction （XRD）. The results showed that SiC film was formed successfully in hydrogen plasma at a substrate temperature of 800℃ . The hydrogen atoms in plasma were found to enhance the production of SiC. Furthermore, the effects of the added CH4 on the formation of film were studied. Introduction of CH4 simultaneously with H2 at the beginning would suppress the formation of the initial layer of SiC due to a carbon-rich environment on the substrate, which would be disadvantageous to the further growth of the SiC film.

Non-thermal Plasma Suppresses Bacterial Colonization on Skin Wound and Promotes Wound Healing in Mice

The present study evaluated the effect of non-thermal plasma on skin wound healing in BalB/c mice.Two 6-mm wounds along the both sides of the spine were created on the back of each mouse（n=80） by using a punch biopsy.The mice were assigned randomly into two groups,with 40 animals in each group：a non-thermal plasma group in which the mice were treated with the non-thermal plasma;a control group in which the mice were left to heal naturally.Wound healing was evaluated on postoperative days（POD） 4,7,10 and 14（n=5 per group in each POD） by percentage of wound closure.The mice was euthanized on POD 1,4,7,10,14,21,28 and 35（n=1 in each POD）.The wounds were removed,routinely fixed,paraffin-embedded,sectioned and HE-stained.A modified scoring system was used to evaluate the wounds.The results showed that acute inflammation peaked on POD 4 in non-thermal plasma group,earlier than in control group in which acute inflammation reached a peak on POD 7,and the acute inflammation scores were much lower in non-thermal group than in control group on POD 7（P0.05）.The amount of granular tissue was greater on POD 4 and 7 in non-thermal group than in control group（P0.05）.The re-epithelialization score and the neovasularization score were increased significantly in non-thermal group when compared with control group on POD 7 and 10（P0.05 for all）.The count of bacterial colonies was 103 CFU/mL on POD 4 and 20 CFU/mL on POD 7,significantly lower than that in control group（109 CFU/mL on POD 4 and 1012 CFU/mL on the POD 7）（P0.05）.It was suggested that the non-thermal plasma facilitates the wound healing by suppressing bacterial colo-nization.

Hydrogen cold plasma for synthesizing Pd/C catalysts: the effect of support-metal ion interaction

It has been found that cold plasma is a facile and environmentally benign method for synthesizing supported metal catalysts, and great efforts have been devoted to enlarging its applications. However, little work has been done to disclose the influence mechanism, which is significant for controllable synthesis. In this work, hydrogen cold plasma was adopted to synthesize a palladium catalyst supported on activated carbon （Pd/C-P） using HzPdC14 as a Pd precursor followed by calcination in hydrogen gas to remove the chlorine ions. The Pd/C-P catalyst was found to be made of larger Pd nanoparticles showing a decreased migration to the support outer surface than that prepared by the conventional thermal hydrogen reduction method （Pd/C-C）. Meanwhile, the pore diameter of the activated carbon support is small （,-~4 nm）. Therefore, Pd/C-P exhibits lower CO oxidation activity than Pd/C-C. It was proposed that the strong interaction between the activated carbon and PdC142-, and the enhanced metal-support interaction caused by hydrogen cold plasma reduction made it difficult for Pd nanoparticles to migrate to the support outer surface. The larger-sized Pd nanoparticles for Pd/C-P may be due to the Coulomb interaction resulting in the disturbance of the metal-support interaction. This work has important guiding significance for the controllable synthesis of supported metal catalysts by hydrogen cold plasma.

C/C基体上ZrB_(2)/SiC复合涂层的抗烧蚀性增强方法

研究高压热流场景下,在碳/碳(C/C)基体表面ZrB_(2)/SiC复合涂层的抗烧蚀性增强方法.在等离子体喷涂ZrB_(2)/SiC复合涂层表面,采用CH3SiCl3-H2-Ar系统,化学气相沉积(CVD)-SiC密封层.对ZrB_(2)/SiC涂层与CVD-SiC密封层的结构演化、烧蚀性能与烧蚀机理进行了研究.结果表明:CVD-SiC密封层增强的ZrB_(2)/SiC复合涂层经过两个600 s循环烧蚀后,仍然具备抗烧蚀性能,表面CVD-SiC由β相向α相转化.CVD-SiC密封层填充了等离子喷涂ZrB_(2)/SiC复合涂层的空隙,限制了氧沿着涂层裂纹和孔洞向C/C基体扩散,进而提高了抗烧蚀性.

等离子弧熔覆Fe-C-B-V系耐磨堆焊层的组织及性能研究

以硼铁、高碳铬铁、钒铁为原料,采用等离子弧熔覆技术在Q345钢表面堆焊Fe-C-B-V系铁基合金。使用金相显微镜、扫描电镜和X射线衍射仪等研究了堆焊层的显微组织;分别使用维氏硬度计和冲击试验机测量堆焊层的显微硬度和冲击韧性。结果表明,堆焊层显微组织主要由马氏体、网格状Fe_(3)(C,B)和Fe_(2)B、弥散分布的碳化物及硼化物等(如VB_(2)、VC、Cr_(2)B)硬质相构成;堆焊层的平均硬度高达904.58 HV10、冲击功为68.5 J。堆焊过程中形成的硼化物、碳化物作为硬质相提高了堆焊层的硬度和耐磨性,形成的碳化钒使组织从鱼骨状变成网格状,细化了晶粒,提高了堆焊层的冲击韧性。

B含量对Fe-Cr-C系堆焊合金组织结构及耐磨性的影响

为了进一步提高Fe-Cr-C系堆焊合金的耐磨性,采用等离子粉末堆焊技术,通过调整堆焊合金粉体中硼铁粉的添加量,在Q235钢表面制备不同B含量的Fe-Cr-C耐磨堆焊合金层,并采用X射线衍射(XRD)、扫描电镜(SEM)、能谱(EDS)及磨粒磨损试验等分析测试方法,探究B添加量对Fe-Cr-C系堆焊合金组织结构、物相组成及耐磨性的影响。结果表明:当B添加量(以硼铁粉计,下同)为0时,堆焊合金的组织由树枝晶状Fe和菊花状(Fe,Cr)_(7)C_(3)+Fe共晶组织组成。随着B添加量的增加,堆焊层中(Fe,Cr)_(7)(C,B)_(3)硬质相的体积分数增加。(Fe,Cr)_(7)(C,B)_(3)相的析出量达到一定值后,初晶B_(0.7)Fe_(3)C_(0.3)相生成。当B添加量为20.0%时,(Fe,Cr)_(2)B硬质相大量析出。堆焊层的硬度整体呈现上升-陡降-上升的趋势,B-10试样的硬度达到63.1 HRC。磨粒磨损试验结果显示,堆焊合金层耐磨性呈现上升-平缓-上升的趋势,B-20试样的耐磨性增至B-0试样的2.8倍。

Disinfection of plasma with ultraviolet C and cross-linked starch iodine

objective:To seek a good method for plasma disinfection to solve the serious problem of viral con tamination of blood and blood products. Methods: A model of plasma disinfection was established by using ultraviolet C (UVC) irradiation and cross-linked starch iodine (CSI) treatment to kill Sindbis virus (SV). The efficacy of plasma virus inactivation was measured by assay of cell infection. The plasma IgG activity was surveyed using different immunohistochemical assays. Results: After being irradiated by 986O J/m2 UVC fol lowed by passage through a column of CSl with a velocity of 0. 25 ml/min, the SV in plasma was reduced to 6. 8 log. No significant inhibition of the activities of the antigen and antibody of IgG were found in the plas ma. Couclusion: The use of UVC in combination with CSI can effectively inactivate SV in the plasma. Thus it is a useful measure for the disinfection of blood products.

Characterization of phase transformation and microstructure of nano hard phase Ti(C,N)-based cermet by spark plasma sintering

By means of optical microscope , scanning electron microscope （SEM） and transmission electron microscope （TEM）, the process of densification, the characterization of phase transformation and the microstructure for spark plasma sintering （SPS） nano hard phase Ti（C,N）-based cermet were investigated. It is found that the spark plasma sintering （SPS） enables the nano hard phase Ti（C,N）-based cermet to densify rapidly, however, the full densification of the sintered samples can not be obtained. The rate of phase transformation is significantly quick. When being sintered at 1 200 ℃ for 8 min, Mo2C is completely dissolved, and TiN dissolves into TiC entirely and disappears. Above 1 200 ℃, Ti（C,N） begins to decompose and the atoms of C and N separate from Ti（C,N） resulting in the generation of N2 and the graphite. Due to the denitrification and the graphitization, the density and the hardness of sintered samples are rather low. The distribution of grain size of the sample sintered at 1 350 ℃ covers a wide range of 90500 nm, and most of the grain size are about 200 nm. The hard phase is not of typical core-rim structure. Oxides on the surface of particles can not be fully removed and present in sample as titanium oxide TiO2. Graphite exists in band-like shape.

Microstructure and wear resistance of Fe-Cr-Ti-C reactive plasma cladding coating

Fe-Cr-Ti-C composite powder was synthesized by precursor carbonization-composition process using the mixture of ferrotitanium, chromium, iron powder and precursor sucrose as raw material. And then the Fe-Cr-Ti-C coating was prepared by reactive plasma cladding method. Microstructure of the samples was observed by scanning electron microscope （SEM）, the phases were determined by X-ray diffraction （XRD）, and the wear resistance was evaluated under dry sliding wear test conditions at room temperature. Results indicate that the composite coating consists of primary austenite and dendritic eutectic austenite, chrysanthemum-shaped eutectic （Cr, Fe ） 7 C3 and TiC carbide. TiC presents the gradient distribution and different shapes in the coating, corresponding to equiaxial structure both in fusion zone and central zone, while it presents dendritic structure on the surface, respectively. The wear mass loss is insensitive to load for the coating while it increases rapidly for Q235 steel base metal in this test. The wear mass loss ofQ235 steel is 14 times as that of the composite coating under applied load of 40 kg.

血气分析检测、D-D及CRP水平用于PTE分型中的临床效果

目的探讨血气分析检测、血浆D-二聚体(D-D)和C反应蛋白(CRP)在血栓性肺栓塞分型中的应用效果。方法选取2021年9月至2022年7月该院74例血栓性肺栓塞患者,以栓塞范围为依据分为甲组(非大面积栓塞,37例)与乙组(大面积栓塞,37例),并选择同期37例健康体检者为对照组,检测3组受检者血气指标、D-D和CRP,分析检测结果。结果甲组及乙组氧分压比对照组低,而CRP及D-D较对照组高,差异有统计学意义(P<0.05);甲组二氧化碳分压比乙组及对照组低,差异有统计学意义(P<0.05);而乙组与对照组二氧化碳分压差异无统计学意义(P>0.05)。甲组氧分压阳性检出率比乙组低,而二氧化碳分压、D-D阳性检出率比乙组高,差异有统计学意义(P<0.05);甲组与乙组CRP阳性检出率差异无统计学意义(P>0.05)。结论血浆D-D、血气分析检测及CRP在血栓性肺栓塞分型诊断中具有较高的应用价值,可为疾病诊断及治疗提供参考,值得临床采纳、推广。

Comparative study of the fabrication of ultrafine Ti(C,N)-based cermets by spark plasma sintering and conventional vacuum sintering

Spark plasma sintering (SPS) and conventional vacuum sintering (VS) wereemployed to fabricate ultrafine Ti(C,N)-based cermets. The shrinkage behavior, microstracture, andporosity and mechanical properties of the samples fabricated by SPS were compared with those of thesamples sintered by VS using optical microscopy, scanning electron microscopy, universal testingmachine, and rockwell tester. The results are as follows: (1) The shrinkage process occurred mainlyin the range of 1000-1300 deg C during the VS process, and only a 0.2 percent linear shrinkage ratioappeared below 800 deg C; during the SPS process, a 60 percent dimensional change occurred below800 deg C as a result of pressure action. (2) By utilizing the SPS technique, it is difficult forobtaining fully dense Ti(C,N)-based cermets. Due to the much existence of pores and un-combinedcarbon, the mechanical properties of the sintered samples by SPS are inferior to sintered ones byVS. (3) grain size of the samples sintered by SPS is still below 0.5 urn, but not by VS; because oflow sintering temperature, there are no typical core/rim structures formed in the sintered samplesby SPS1; the main microstructures of the sintered samples by SPS2 are a white core/grey shellstructure, whereas by VS show a typical black core/grey shell structure.

Spark Plasma Sintering Properties of Ultrafine Ti(C,N)-based Cermet

Ultrafine Ti(C,N)-based cermet was sintered by SPS from 1050℃ to 1450℃ and its sintering properties,such as porosity,mechanical properties and phase transformation,were investigated by optical microscopy (OM),scanning electron microscopy (SEM),X-ray diffraction (XRD),and differential scanning calorimeter (DSC).It is found that the spark plasma sintering properties of Ti(C,N)-based cermet differ from those of conventional vacuum sintering.The liquid phase appearance is at least lower by 150℃ than that in vacuum sintering.The porosity decreases sharply below 1 200℃ and reaches minimum at 1 200℃,and afterwards it almost keeps invariable and no longer increases.SPS remarkably accelerates the phase transformation of Ti(C,N)-based cermet and it has a powerful ability to remove oxides in Ti(C,N)-based cermets.Above 1 350℃,denitrification occurred.Fresh graphite phase formed above 1 430℃.Both the porosity and graphite are responsible for the poor TRS.

不同标本类型中超敏C反应蛋白检验结果在脑瘫儿童感染性疾病治疗中的一致性分析

目的探讨不同标本类型的超敏C反应蛋白检验结果在脑瘫儿童感染性疾病治疗中的效果和一致性分析。方法本次研究将试验时间段设置在2022年8月至2023年12月,在该时段内将在我院接受感染性疾病治疗的91例脑瘫儿童作为研究对象,研究人员针对所有儿童进行超敏C反应蛋白检验,确认其感染状况,在检验时采用不同标本类型进行超敏C反应蛋白检验,探讨差异。研究人员采集血液标本,用全血与血浆进行检验,判断其中超敏C反应蛋白含量,用以探讨不同样本的超敏CRP数据结果准确度。结果在检验中发现,以0~10 mg/L为判断指标,全血超敏CRP水平小于10 mg/L的例数共有15例,血浆超敏CRP水平小于10mg/L的例数共有18例。全血超敏CRP水平的均值为(24.58±2.24)mg/L。血浆超敏CRP水平的均值为(24.27±2.23)mg/L,组间数据差异不显著(P>0.05)。结论全血超敏CRP检验在数据中展现出较高的敏感度。两种标本类型检验在平均CRP水平上相似,没有显著性差异,但全血超敏CRP检验结果在感染性指标方面具有一定的敏感性优势,全血检验的方法的准确度更高,更推荐使用全血标本检验。