Identification of a LMNA (c.646C>T) variant by whole-exome sequencing in combination with a dilated cardiomyopathy (DCM) related gene filter in a family with familiar DCM

Dilated cardiomyopathy（DCM）is characterized by the dilated heart chambers and reduced systolic function in the absence of specific aetiology[1].Approximately one third of DCM cases are hereditary.In recent years,DCM concomitant with arrhythmias and sudden death resulting from gene mutation has been widely

Facies logging identification of intermediate-basic volcanic rocks in Huoshiling Formation of Songliao Basin

Volcanic oil and gas reservoirs are generally buried deep,which leads to a high whole-well coring cost,and the degree of development and size of reservoirs are controlled by volcanic facies.Therefore,accurately identifying volcanic facies by logging curves not only provides the basis of volcanic reservoir prediction but also saves costs during exploration.The Songliao Basin is a‘fault-depression superimposed’composite basin with a typical binary filling structure.Abundant types of volcanic lithologies and facies are present in the Lishu fault depression.Volcanic activity is frequent during the sedimentary period of the Huoshiling Formation.Through systematic petrographic identification of the key exploratory well(SN165C)of the Lishu fault-depression,which is a whole-well core,it is found that the Huoshiling Formation in SN165C contains four facies and six subfacies,including the volcanic conduit facies(crypto explosive breccia subfacies),explosive facies(pyroclastic flow and thermal wave base subfacies),effusive facies(upper and lower subfacies),and volcanogenic sedimentary facies(pyroclastic sedimentary subfacies).Combining core,thin section,and logging data,the authors established identification markers and petrographic chart logging phases,and also interpreted the longitudinal variation in volcanic petro-graphic response characteristics to make the charts more applicable to this area's volcanic petrographic interpretation of the Huoshiling Formation.These charts can provide a basis for the further exploration and development of volcanic oil and gas in this area.

DNA barcoding of fishes from Zhoushan coastal waters using mitochondrial COI and 12S rRNA genes

Accurate species identification is a key component of biodiversity research.DNA barcoding is an effective molecular method used for fish species identification.We aimed to study the DNA barcoding of fish in Zhoushan coastal waters,explore the differences and applicability of two gene fragments(12S rRNA and COI)of DNA barcoding in fish species identification,and established a comprehensive fish barcoding reference database.Two hundred and eighty-seven captured fish samples from Zhoushan coastal waters were identified using morphological characteristics and DNA barcoding.A total of 26412S rRNA sequences(belonging to eight orders,31 families,55 genera,and 66 species)and 188 COI sequences(belonging to seven orders,30 families,48 genera,and 58 species)were obtained.The lengths of the 12S rRNA sequences ranged from 165 to 178 bp,and the guanine-cytosine(GC)content was 45.37%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.10%and 26.66%,respectively.The length of the COI sequence ranged 574–655 bp,and the content of GC was 45.97%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.16%and 27.45%,respectively.The minimum interspecific genetic distances of 12S rRNA and COI(1.23%and 1.86%)were both greater than their maximum intraspecific genetic distances(2.42%and 8.66%).Three molecular analyses(NJ tree,ABGD,and GMYC)were performed to accurately identify and delineate species.Clustering errors occurred when the 12S rRNA sequences were delimited using the NJ tree method,and the delimitation results of ABGD and GMYC are consistent with the final species identification results.Our results demonstrate that DNA barcoding based on 12S rRNA and COI can be used as an effective tool for fish species identification,and 12S rRNA has good application prospects in the environmental DNA(eDNA)metabarcoding of marine fish.

Soil pore identification with the adaptive fuzzy C-means method based on computed tomography images

The complex geometry and topology of soil is widely recognised as the key driver in many ecological processes. X-ray computed tomography (CT) provides insight into the internal structure of soil pores automatically and accurately. Until recently, there have not been methods to identify soil pore structures. This has restricted the development of soil science, particularly regarding pore geometry and spatial distribution. Through the adoption of the fuzzy clustering theory and the establishment of pore identification rules, a novel pore identification method is described to extract pore structures from CT soil images. The robustness of the adaptive fuzzy C-means method (AFCM), the adaptive threshold method, and Image-Pro Plus tools were compared on soil specimens under different conditions, such as frozen, saturated, and dry situations. The results demonstrate that the AFCM method is suitable for identifying pore clusters, especially tiny pores, under various soil conditions. The method would provide an optional technique for the study of soil micromorphology.

A New Furost-20(22)-ene Oligoglycoside from Asparagus cochinchinensis

A new furost-20(22)-ene oligoglycoside named as aspacochioside C was isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Its structure was elucidated to be 26-O-b- D-glucopyranosyl-(25S)-5b-furost-20(22)-en-3b,26-diol-3-O-a-L-rhamnopyranosyl-(14)-b-D- glucopyranoside on the basis of spectroscopic techniques including 1D and 2D NMR experiments.

An input signal used in process identification - chaos sequence

The sequences which consist of any segment of a chaos sequence are called asC-sequences. These sequences could be used as a kind of input signals to replace M-sequences in theprocess identification. This substitution is theoretically proved to be feasible. InverseC-sequences are created in a way similar to inverse M-sequences to solve the problem thatC-sequences have non-ideal balance property, that is, the numbers of '0' and '1' are unequal.Besides its good pseudo-random property, the sequences have other advantages such as easy togenerate, varieties of the segment and adjustable cycle time.

Identification of Coptis chinensis and Forsythia sus-pensa from Qingwen Baidu Granules by TLC

[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium liquid （ 12：6：3：3：1 ） as the developer, the samples were outspread in the cylinder with sat- urated ammonia steam by thin layer chromatography （TLC）, and the existence of C. chinensis and berberine hydrochloride was checked under UV lamp. With tri- chloromethane-methanol （5：1） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 105 ℃ for 5 min to check the existence of F. suspense under natural light. [Result] Qingwen baidu granules had the same spots with C. chinensis control and berberine hydrochloride at the same Rf value under 365 nm UV lamp; Qingweu baidu granules had the same spots with F. suspensa control at the same Rfvalue under natural light. [ Condusion] A TLC method detecting C. chinensis and F. suspensa in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of C. chinensis and F. suspensa. It could be well applied to control the quality of Qingwen baidu granule.

Construction of Controllable Expression Vector of Mus musculus Growth Hormone (GH) shRNA and Its Gene Si- lencing Efficiency Detection

Growth hormone （GH） is a polypeptide hormone secreted by the pituitary, which promotes animal growth, muscle development, metabolism regulation and other important physiological functions. In this study, a pair of mGH short hairpin RNA （shRNA） was designed according to mouse （ Mus musculus） GH mRNA sequence; pSingle-tTS-mGH shRNA-RFP, an integrated controllable expression vector of mGH shRNA, was constructed successfully. The recombinant vector was transfected into mouse pituitary tumor cell line AtT-20. After addition of doxycyelin （ DOX）, the expression of red fluorescent protein （RFP） was observed un- der a fluorescent microscope. The expression level of mGH in cells was detected by quantitative Realtime PCR （qRT-PCR） and Western blot. After DOX induction, the relative expression level of GH mRNA in cells transfected with GH shRNA was reduced by about 70% compared with that in DOX-free group and other control groups, exhibiting extremely significant differences （P 〈 0.01 ） ; moreover, the relative expression level of GH protein was reduced by about 90% ; the expression level of GH mRNA and GH protein exhibited no significant difference among other groups （P 〉 0.05）. In this study, a controllable expression vector of GH shRNA with high gene silencing efficiency was constructed successfully, which could be used to reveal GH autocfine / paracrine interactions and analyze functions of GH gene in growth, development and disease occurrence of animals by regulating GH expression levels.

基于LOF-FCM算法的船舶航行数据识别

针对传统船舶自动识别系统数据在清洗异常数据和提取停留数据时分别采用不同的识别方式、类型判断阈值需要人为设定、识别效率不佳的局限性,首次提出了一种船舶航行轨迹中停留及异常数据的一体化检测方法。通过分析航行路线的3种数据(停留、异常和航行)异常因子特征,提出基于LOF-FCM的船舶航行数据、停留数据和异常数据一体化检测算法。实验对3类数据进行了识别,模型识别准确率达到了92.69%,有效提高了异常、停留、航行数据的识别能力。结果表明所提方法可一次性实现AIS数据中3种数据的检测,能高效分离出正常船舶航行数据,具有良好的工程应用价值。

南极土壤嗜冷真菌Chrysosporium sp.C_(3438)活性代谢产物C_(3438)A的分离及结构鉴别

以对精原细胞法是否有活性作为筛选模型 ,对南极土壤嗜冷真菌 Chrysosporium sp. C3 4 3 8经低温发酵的活性产物进行了深入的化学研究 ,首次从南极土壤微生物代谢产物中分离得到

大麦非特异性磷脂酶C基因家族全基因组鉴定及苗期胁迫表达分析

【目的】非特异性磷脂酶C(non-specific phospholipase C,NPC)是磷脂酶C的一种,在植物生长发育和响应非生物胁迫等方面发挥重要作用。在大麦全基因组范围内鉴定NPC基因家族成员,分析相关基因表达特点,为大麦NPC基因功能研究及遗传改良提供理论依据。【方法】基于EnsemblPlants数据库收录的大麦全基因组数据,利用生物信息学的方法对大麦NPC基因家族进行鉴定,并对大麦NPC基因家族的理化特性、亚细胞定位、系统进化关系、基因结构、蛋白结构域和启动子顺势元件等进行分析。利用转录组数据和实时荧光定量PCR分析HvNPC基因在不同组织以及苗期在低温、干旱、盐胁迫下的表达特征。【结果】在大麦基因组中,共鉴定了5个非特异性磷脂酶C基因家族成员,其编码蛋白与其他植物的NPC基因结构相似,均具有Phosphoesterase结构域;HvNPC蛋白序列长度为477-553 aa,等电点为5.22-8.83,分子量为53.12-61.33 kD;亚细胞定位预测HvNPC基因定位于叶绿体、液泡膜、细胞质中;HvNPC启动子区含有大量与非生物胁迫有关的顺势作用元件。实时荧光定量PCR分析证实HvNPC基因受干旱、高盐及低温胁迫诱导表达,其中HvNPC2、HvNPC3和HvNPC5受低温和干旱胁迫诱导表达;HvNPC3和HvNPC5受盐胁迫诱导表达。【结论】在大麦基因组中共鉴定5个HvNPC基因,HvNPC基因成员具有器官表达特异性,并且不同基因对非生物胁迫响应不同。

Roux-en-Y gastric bypass for Chinese type 2 diabetes mellitus patients with a BMI,28kg/m^2:a multi-institutional study

Roux-en-Y gastric bypass surgery（RYGB） has been demonstrated to be successful for treating type-II diabetes2mellitus（T2DM） patients with a body mass index（BMI）,30 kg/m,but reports of RYGB for T2 DM patients with22 a BMI,28 kg/mare lacking.T2 DM patients with a BMI,28 kg/mwere prospectively recruited to participate in this study in four hospitals.The endpoint was T2 DM remission（defined by fasting blood glucose（FBG） level,110 mg/d L and hemoglobin（Hb）A1c level,6.0% at 12 months postoperatively）.Predictors of remission were investigated by univariate and multivariate analyses.Eighty-six patients were assessed.Eighty-five patients underwent RYGB,with one conversion to open surgery.We compared the values of various variables before and after2 surgery.The mean BMI decreased from 24.68±2.12 to 21.72±2.43 kg/m（P,0.001）.Fifty-eight（67.4%） patients were not treated by drugs or insulin after surgery,and 20 patients（23.3%） had complete remission of T2 DM at12 months after surgery with an acceptable number of complications.The mean Hb A1 c level in the remission group was significantly lower than that in the non-remission group.Patients with a higher weight,lower Hb A1 c level,higher C-peptide level,and higher FBG level were more likely to have T2 DM remission in multivariate2 analyses.In conclusion,RYGB was effective and safe for treating T2 DM patients with a BMI,28 kg/m.Complete remission can be predicted by cases having a higher weight,lower Hb A1 c level,higher C-peptide level,and higher FBG level.

Antagonistic Effects of Sphingomonas and Pseudomonas aeruginosa on 4 Kinds of Pathogenic Bacteria of Ginseng

[Objectives]To explore effective biocontrol methods for diseases in the process of ginseng cultivation,and develop an efficient and environmentally friendly biocontrol agent.[Methods]In this study,2 strains were isolated from biogas slurry,and Cylindrocarpon destructans(XF),Fusarium solani(GF),Botrytis cinerea Pers(HM)and Alternaria panax Whetz(HB)were used as test materials.The strains were isolated and identified by dilution plate method,16S rDNA sequence identification method,confrontation culture method,filter paper method and ultraviolet spectrophotometer method,and the bacteriostatic activity and bacteriostatic rate were tested.[Results]Strain 15(Sphingomonas)and strain 19(Pseudomonas aeruginosa)were screened out through identification and analysis,and they grew stably within 8-10 d.The bacteriostatic rates of strain 15 against A.panax and B.cinerea were 47.37%and 43.40%,respectively,and the bacteriostatic rates of strain 19 against A.panax and B.cinerea were 62.30%and 63.27%,respectively.The bacteriostatic activity of the extract of strain 19 increased with the increase of OD_(600) value,and the bacteriostatic effect was optimal when the OD_(600) value was in the range of 0.8-1.0,up to 70%,so it had a strong biocontrol potential.[Conclusions]This experiment provides convenience for more effective inoculation,establishes a fast,simple and accurate method for the determination of the best bacteriostatic rate of P.aeruginosa culture solution to HM,and lays a foundation for large-scale culture of P.aeruginosa culture solution.Besides,it is expected to provide a theoretical basis for the efficient control of ginseng B.cinerea in field production,use it for the prevention and control of ginseng shoot diseases,and provide a reference for the efficient and diverse development of biocontrol agents for ginseng shoot diseases.

罗兰C接收机信号处理关键技术及发展

罗兰C接收机性能对系统的导航授时定位精度有显著影响。论文在介绍罗兰C接收机工作原理的基础上,对信号捕获、天地波识别、周期识别和ASF修正等信号处理关键技术进展进行了分析研究。研究表明,现有罗兰C接收机信号处理技术多针对单个问题进行理论研究且存在一定局限性,实际整体接收性能还有待进一步检验。通过对当前研究现状的分析比较,论文从高动态捕获、联合算法研究、非线性时变特性研究、ASF数据更新四个方面重点探讨了我国罗兰C接收机信号处理技术的发展方向。

基于改进FCM和PSO-SVM的焊接缺陷识别

为实现海洋工程钢结构件焊接接头缺陷的客观、智能化分类,本文以其数字射线检测图像作为研究对象,进行基于改进的模糊C均值聚类算法(FCM)和粒子群优化支持向量机(PSO-SVM)的缺陷识别研究。首先,基于限制对比度直方图均衡化去除原始图像中干扰噪声,引入像素点加权系数ω改进FCM进行图像分割;然后,基于灰度共生矩阵提取图像纹理特征,利用主成分分析法进行特征数据降维,将粒子群优化与支持向量机分类相结合进行参数寻优,建立纹理特征与缺陷类型间的连续变量分类模型;最后,以多人工综合完全正确的评价结果验证缺陷识别模型的有效性和准确性。结果表明:所训练的识别模型准确率为96.11%,经验证其识别准确率约为95.2%。与未经限制对比度自适应直方图均衡化(CLAHE)增强的模型、反向传播(BP)神经网络模型对比,该模型可以很好地实现常见缺陷的识别,且误差小,可应用于船用钢数字射线焊接缺陷识别领域。

肠日本血吸虫病与肠癌组织c-erbB-2及DPC4表达水平的研究

目的探讨慢性日本血吸虫病与大肠癌之间的关系。方法用免疫组化两步法及SP法分别检测1 5例正常结肠黏膜,1 5例单纯血吸虫病结肠黏膜,2 0例血吸虫病并大肠癌,2 0例无血吸虫病大肠癌的c-erbB-2及DPC 4的表达。结果c-erbB-2在4组中均有一定程度的表达,但在血吸虫病肠黏膜中的表达最高(P<0.0 5)。DPC 4亦在4组中有一定程度的表达,但在正常肠黏膜的表达水平最高(P<0.0 5)。结论慢性日本血吸虫病与大肠癌之间存在一定的关系;慢性日本血吸虫病引起黏膜上皮恶变时,c-erbB-2,DPC 4表达水平的改变可能是一早期分子事件。

HBV前C区部分序列亚克隆与FQ-PCR标准曲线的制备

目的探讨HBV前C区基因用于乙型病毒性肝炎早期诊断价值和意义并基于此靶基因制备荧光定量PCR标准曲线。方法设计特异性引物,将pBR322-HBV质粒前C区部分序列PCR产物AT亚克隆至pBS-T载体,提取和纯化质粒DNA,制作标准品并在FQ-PCR仪上得到标准曲线,进而对精确度和重复性进行统计分析。结果获得了预期希望的质粒。荧光定量PCR标准曲线的相关系数为0.995,线性范围5×103 copies/μl^5×109 copies/μl。批内、批间变异系数值均小于5%,精确度高,重复性好。结论HBV前C区基因用于乙型病毒性肝炎早期检测和诊断有一定价值和意义。且FQ-PCR标准曲线的成功制备,临床早期检测乙肝病毒感染奠定了基础。

实时荧光定量PCR检测白血病患者WT1基因表达及临床意义

目的研究白血病患者WT1基因的表达水平及与临床预后的关系。方法建立实时荧光定量PCR检测WT1基因表达技术,并分析65例白血病患者WT1基因的表达水平及与临床预后的关系。结果建立的实时荧光定量PCR方法的标准曲线相关系数>0.99。急性髓性白血病(AML)和急性淋巴细胞白血病(ALL)患者WT1基因表达水平较正常对照组均显著升高(P=0.001)。慢性髓性白血病(CML)急变期患者WT1基因表达水平显著高于慢性期。WT1表达量与急性白血病发病时外周血白细胞计数、血红蛋白、血小板无明显相关关系。AML和ALL患者中WT1基因高表达患者与低表达病例CR率差异无显著性。急性白血病患者治疗缓解后WT1的表达量较治疗前显著下降(P=0.032)。随访显示WT1持续高表达或下降后又上升的患者呈现难治及复发。结论实时荧光定量PCR技术检测急性白血病患者WT1基因表达量可预测难治复发及用于MRD的检测。

一株高产PLC的CW-W-90-3菌的鉴定

198 9年 ,筛选了 1株高产 phospholipaseC(PLC)的CW W 90 3菌株[1,2 ] ,据其形态特征、生理生化反应 ,初步将其归于弧菌科气单胞菌属[3 ] ,由于该菌株的许多生理生化特性与粘质沙雷氏菌相同。但其极生单鞭毛和无色素及少许生理生化特性与粘质沙雷氏菌相异。后经AutomatedBacteriaIdentificationSystem BiologMicroStationSystem检测 96种C源和N源的利用及其个体群体发育 ,说明其与粘质沙雷氏菌 (Serratiamarcescens)相符 ;并在基因组水平上研究该菌株的系统发育 ,从分子水平上对该菌株进行 16SrRNA序列分析煌同源性比较。根据CW W 90 3菌株 16SrRNA与GeneBank数据库中Serratiamarcescens的 16SrRNA的序列具有 99%的同源性 ,终将CW W 90 3菌株鉴定为粘质沙雷氏菌武汉株 (SerratiamarcescensWuhanstrain)。

响应面法优化樟芝液态发酵产Antrodin C

分析樟芝液态发酵菌丝体中的活性代谢产物Antrodin C,并以此化合物为目标,采用Plackett-Burman设计和Box-Behnken中心组合响应面分析,对樟芝液态发酵产Antrotin C培养基进行统计学筛选和优化。结果表明:葡萄糖、黄豆粉和MgSO4对Antrodin C的合成影响最为显著。在葡萄糖72.0g/L、黄豆粉5.91g/L、MgSO40.614g/L时,樟芝液态发酵产Antrodin C最大预测值为178.59mg/L。验证实验Antrodin C实际产量达到(177.83±0.32)mg/L,表明实验建立的模型能较好地预测实际发酵产Antrodin C情况。通过对培养基的优化,樟芝液态发酵Antrodin C产量比优化前(95.72mg/L)提高了85.8%。