Study on the Gene Transcription Level of Hippocampus NGF in C_(57) Mouse Development

β-Nerve growth factor (β NGF) mRNA levels in hippocampa from C57BL/6J mice of different ages were compared by semi-quantitative RT-PCR method, taking β-actin gene as an internal control. The levels of 6-and 12-month-old C57 mice were much lower compared with that of the l-month-old mouse. However, there was no significant difference between these two groups. This result suggests that gene transcription level of hippocampus β NGF in a C57, mouse descends evidently as it grows up. Base T at Site 294 of β NGF cDNA was substituted by C in the C57, mouse, as revealed by DNA sequencing for the first time. Nevertheless, this polymorphism of nucleotide did not make any difference in amino acid composition.

外语教师发展的有效策略——评介Jack C.Richards所著Professional Development for Language Teachers:Strategies for Teacher Learning

J.C.Richards教授在英语语言教学和教师培训领域的实践经历与理论研究,对其本人关于"语言教师发展"思想体系的构建起到了积极的促进作用。他于2005年出版的Professional Development for Language Teachers:Strategies for Teacher Learning(《语言教师专业发展:教师学习策略》)一书论述了"教师教育"的本质,分析了关于"教师发展"的六个重要观点,尤其是对语言教师发展策略进行系统性阐述,以章节形式对十一种发展策略进行全面细致的介绍,并附有具体事例、教师反思、参考书目等。该书是作者对英语语言教师发展领域的理论研究及实践经验的总结,其内容及框架在注重理论知识的科学性和系统性的同时,也突出了实践操作的必要性和可行性,可以作为我国外语教师开展职业发展、提升职业素质的有益参考。

Action Research——the Way to Enhancing Teacher Professional Development

This paper introduces the notion of action research in its international academic context. Action research is potentially beneficial to both pre-service teachers and in-service teachers in their professional development and it should play an important role in the curriculum renewal of English teaching methodology in China.

Conversion of carbon dioxide to valuable petrochemicals:An approach to clean development mechanism

The increase of atmospheric carbon dioxide and the global warming due to its greenhouse effect resulted in worldwide concerns. On the other hand, carbon dioxide might be considered as a valuable and renewable carbon source. One approach to reduce carbon dioxide emissions could be its capture and recycle via transformation into chemicals using the technologies in C1 chemistry. Despite its great interest, there are difficulties in CO2 separation on the one hand, and thermodynamic stability of carbon dioxide molecule rendering its chemical activity low on the other hand. Carbon dioxide has been already used in petrochemical industries for production of limited chemicals such as urea. The utilization of carbon dioxide does not necessarily involve development of new processes, and in certain processes such as methanol synthesis and methane steam reforming, addition of CO2 into the feed results in its utilization and increases carbon efficiency. In other cases, modifications in catalyst and/or processes, or even new catalysts and processes, are necessary. In either case, catalysis plays a crucial role in carbon dioxide conversion and effective catalysts are required for commercial realization of the related processes. Technologies for CO2 utilization are emerging after many years of research and development efforts.

Relationship between monocularly deprivation and amblyopia rats and visual system development

Objective:To explore the changes of lateral geniculate body and visual cortex in monocular strabismus and form deprived amblyopic rat,and visual development plastic stage and visual plasticity in adult rats.Methods:A total of 60 SD rats ages 13 d were randomly divided into A,B,C three groups with 20 in each group,group A was set as the normal control group without any processing,group B was strabismus amblyopic group,using the unilateral extraocular rectus resection to establish the strabismus amblyopia model,group C was monocular form deprivation amblyopia group using unilateral eyelid edge resection+lid suture.At visual developmental early phase(P2S),meta phase(P3S),late phase(P45)and adult phase(P120),the lateral geniculate body and visual cortex area 17 of five rats in each group were exacted for C-fos Immunocytochemistry.Neuron morphological changes in lateral geniculate body and visual cortex was observed,the positive neurons differences of C-fos expression induced by light stimulation was measured in each group,and the condition of radiation development of P120 amblyopic adult rats was observed.Results:In groups B and C,C-fos positive cells were significantly lower than the control group at P25(P<0.05),there was no statistical difference of C-fos protein positive cells between group B and group A(P>0.05),C-fos protein positive cells level of group B was significantly lower than that of group A(P<0.05).The binoculus C-fos protein positive cells level of groups B and C were significantly higher than that of control group at P35,P4S and P120 with statistically significant differences(P<0.05).Conclusions:The increasing of C-fos expression in geniculate body and visual cortex neurons of adult amblyopia suggests the visual cortex neurons exist a certain degree of visual plasticity.

Existing Problems of Agricultural Development in China and Recommendations

In 2012,China's total grain yield reached 590 million tons and realized nine consecutive years of growth. This is huge historic achievement of China and also rare in development history of world agriculture. However,at the same time of making great achievements,China's agriculture is still confronted with many problems. On the basis of extensive survey,it pointed out 10 outstanding problems: farmland,labor,livestock power,water conservancy,input,agricultural disaster,ecological and environmental problem,waste of resources,economic scale and economic benefits. These problems have brought great adverse influence on China's agricultural development. In view of these problems,to realize sustainable,stable and healthy development of agriculture in the new period,it came up with following recommendations:( i)deepening the understanding;( ii) enforcing laws strictly;( iii) enhancing training;( iv) increasing benefits;( v) saving resources;( vi) protecting environment;( vii) developing science and technology;( viii) consolidating foundation.

PtrDJ1C,an atypical member of the DJ-1 superfamily,is essential for early chloroplast development and lignin deposition in poplar

The nuclear-encoded factors and the photosynthetic apparatus have been studied extensively during chloroplast biogenesis.However,many questions regarding these processes remain unanswered,particularly in perennial woody plants.As a model material of woody plants,poplar not only has very significant value of research,but also possesses economic and ecological properties.This study reports the Populus trichocarpa DJ-1C(PtrDJ1C)factor,encoded by a nuclear gene,and a member of the DJ-1 superfamily.PtrDJ1C knock-out with the CRISPR/Cas9 system resulted in different albino phenotypes.Chlorophyll fluorescence and immunoblot analyses showed that the levels of photosynthetic complex proteins decreased significantly.Moreover,the transcript level of plastid-encoded RNA polymerase-dependent genes and the splicing efficiency of several introns were affected in the mutant line.Furthermore,rRNA accumulation was abnormal,leading to developmental defects in chloroplasts and affecting lignin accumulation.We concluded that the PtrDJ1C protein is essential for early chloroplast development and lignin deposition in poplar.

Analysis of Coordination between the Public Service in Rural Areas and Socio-economic Development——A Case Study of Sichuan Province

Taking Sichuan Province as an example,by using the overall evaluation function of Sichuan's rural public service equalization development level and rural socio-economic development level,we conduct profound analysis on coordination between public service in Sichuan's rural areas and socio-economic development from 2003 to 2008.The results show that the coordination between rural public service and socio-economic development in Sichuan Province is not high,and the equalization phenomenon of rural public service construction and socio-economic development is very prominent.The equalization development of public service in rural areas of Sichuan Province from 2003 to 2008 lags behind socio-economic development.The coordination between public service equalization system in rural areas of Sichuan Province and socio-economic development system abates continuously;the coordination between infrastructure and socio-economic development increases slowly;the coordination between education and socio-economic development declines sharply;the coordination between public culture and socio-economic development tends to decrease;the coordination between ecological environment construction and socio-economic development decreases continuously with great amplitude;the coordination between public health and socio-economic development decreases continuously;the coordination between science and technology and socio-economic development lingers at low level;the coordination between social security and employment,and socio-economic development increases in fluctuation,but with small amplitude.

Spatial and temporal expression of c-mos in mouse testis during postnatal development

Aim： To immunolocalize the c-mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development. Methods： Semi-quantitative reverse transcription-polymerase chain reaction （RT- PCR） and in situ hybridization techniques were used to examine c-mos mRNA and indirect immunofluorescence was used to localize c-Mos protein in mouse testis on postnatal days 14, 21, 25, 28, 30, 35, 49 and 70. Results： c-mos mRNA remained low on postnatal days 14-21, increased abruptly from day 25 and peaked on day 30. Its levels decreased a little on day 35 and became almost stable thereafter until day 70. c-mos mRNA was localized in the nucleus and cytoplasm of the spermatocytes and round spermatids. The nuclear staining was much stronger than the cytoplasmic staining. Using a polyclonal anti-c-Mos antibody, Western blotting detected a single band at 43 kDa in testis lysate, c-Mos protein was exclusively localized to the elongating spermatids and was first detected on postnatal day 30. The number of c-Mos-positive spermatids increased progressively till day 49 and stabilized thereafter. Conclusion： The c-mos gene displays a spatial and temporal expression pattern in the mouse testis during postnatal development at both the mRNA and protein level. This suggests that c-mos might play important roles in spermatogenesis. （Asian J Androl 2008 Mar; 10： 277-285）

An Empirical Study of Economic Growth,Human Capital and Financial Development in Qingdao City

Human capital and finance can boost economic growth,and this paper performs an empirical test of economic growth,human capital and financial level,and finds that there is a long-term stable dynamic equilibrium relationship between the three.Results show that the finance and human capital in Qingdao City go through six stages( " imbalance-on the verge of imbalance-narrow coordination-primary coordination-intermediate coordination-well coordination"),and during the coordination process,human capital significantly lags behind.Therefore,it is necessary to increase human capital,accelerate the coordination between finance and human capital,and further promote the development of economic level.

Application of Responsible Tourism in Development of Tourism Destinations——A Case Study of Shangqiu City

The development of responsible tourism is always a focus of foreign scholars' attention,but the method of developing responsible tourism implemented in China's Shangqiu area,is the first of its kind. From the perspective of interests of many parties of tourists,tourism developers,government,and related group of people,this article puts forth the ideas of developing the cultural heritage tourism resources based on the basic ideas and goals of responsible tourism development,and especially researches the feasibility of specific implementation program. Understanding this will be of practical significance to the development of similar tourism resources,the protection and management of cultural relics in China.

Development of a risk score to guide targeted hepatitis C testing among human immunodeficiency virus patients in Cambodia

BACKGROUND The World Health Organization recommends testing all human immunodeficiency virus(HIV)patients for hepatitis C virus(HCV).In resource-constrained contexts with low-to-intermediate HCV prevalence among HIV patients,as in Cambodia,targeted testing is,in the short-term,potentially more feasible and cost-effective.AIM To develop a clinical prediction score(CPS)to risk-stratify HIV patients for HCV coinfection(HCV RNA detected),and derive a decision rule to guide prioritization of HCV testing in settings where‘testing all’is not feasible or unaffordable in the short term.METHODS We used data of a cross-sectional HCV diagnostic study in the HIV cohort of Sihanouk Hospital Center of Hope in Phnom Penh.Key populations were very rare in this cohort.Score development relied on the Spiegelhalter and Knill-Jones method.Predictors with an adjusted likelihood ratio≥1.5 or≤0.67 were retained,transformed to natural logarithms,and rounded to integers as score items.CPS performance was evaluated by the area-under-the-ROC curve(AUROC)with 95% confidence intervals(CI),and diagnostic accuracy at the different cut-offs.For the decision rule,HCV coinfection probability≥1% was agreed as test-threshold.RESULTS Among the 3045 enrolled HIV patients,106 had an HCV coinfection.Of the 11 candidate predictors(from history-taking,laboratory testing),seven had an adjusted likelihood ratio≥1.5 or≤0.67:≥50 years(+1 point),diabetes mellitus(+1),partner/household member with liver disease(+1),generalized pruritus(+1),platelets<200×10^(9)/L(+1),aspartate transaminase(AST)<30 IU/L(-1),AST-to-platelet ratio index(APRI)≥0.45(+1),and APRI<0.45(-1).The AUROC was 0.84(95%CI:0.80-0.89),indicating good discrimination of HCV/HIV coinfection and HIV mono-infection.The CPS result≥0 best fits the test-threshold(negative predictive value:99.2%,95%CI:98.8-99.6).Applying this threshold,30%(n=926)would be tested.Sixteen coinfections(15%)would have been missed,none with advanced fibrosis.CONCLUSION The CPS performed well in the derivation cohort,and bears potential for other contexts of low-to-intermediate prevalence and little onward risk of transmission(i.e.cohorts without major risk factors as injecting drug use,men having sex with men),and where available resources do not allow to test all HIV patients as recommended by WHO.However,the score requires external validation in other patient cohorts before any wider use can be considered.

Potential Problems in Land Development Intensity under Current Control and Corresponding Strategies:A Case Study of Xingtang County

Cities renew and develop constantly. In the context of scarce land resources, China’s cities in thehigh-density and high-development construction mode will indirectly affect the road network load aroundthe cities. “Land development intensity”, as an important content in China’s current regulatory plan, is akey factor to realize and guarantee the rational allocation and utilization of land resources in cities. TakingXingtang County of Shijiazhuang City as an example, this paper made an in-depth analysis and summaryof its current characteristics and existing problems, and put forward reasonable solutions and relevantoptimization strategies accordingly, attempted to form a reasonable land use and planning layout and reducetraffi c pressure, so as to provide experience and reference for other cities to solve such problems.

Synthesis of Attaining Sustainable Development Goal 6.5: A Case Study of Mano River

The Mano River is a transboundary river that runs through Côte d’Ivoire, Liberia, Guinea, and Sierra Leone. According to a 2018 United Nation report on Sustainable Development Goal (SDG) 6.5, which aims to improve the adoption of cohesive water resource management by 2030;the impact of this goal on the Mano River management is minimal. The research summarizes previous studies using an integrative literature review method, drawing general conclusions and identifying unsolved problems with respect to water resources management. The research finding demonstrated that existing water stress and poor management as exacerbated by socioeconomic practices in the region are the major threats to achieve SDG 6.5. As a result of these activities, long-term management of the river basins has become extremely difficult. The research informs a discussion to achieve cooperative water resource management, which is best achieved by shared collaboration and shared interests as described by SDG 6.5.

Power Grid Development in 60 Years since the Foundation of P.R.C.

Foreword Since the foundation of P.R.C.,the China's power grid develops with a high rate.By now,grids in all provinces except Xinjiang,Tibet,Hainan and Taiwan are AC/DC interconnected together,forming the structure of power grid interconnection in the whole country.

UG Programming Development and Digital Design of Globoidal Indexing CAM Mechanism

In the design process of globoidal CAM mechanism,there are some shortcomings such as long structure design cycle,low efficiency,and no modification flexibility of parameters of similar parts.NX Open classic application programming interfaces(APIS)can be connected with Microsoft Visual Studio(VS).Based on NX Open application,users can develop menus,toolbars,dialog boxes and other tools to realize interactive design.Users can also call input parameters through the dialog box callback function,then activate the corresponding control application(callback function)by C++programming language and NX Open API complete code.Based on the secondary development function of UG software and VS,a digital design system suitable for high efficiency modeling of globoidal indexing CAM mechanism was developed to complete the human-machine interface interaction design.The results show that comparing the digital design system with the traditional design,the system after parametric design can not only greatly shorten the design time,but also reduce the tedious repetitive work,which proves the superiority and feasibility of the digital design.

Capacity Development of National Reference Centers (NRC) to Face the Challenges of Emerging Infections in Côte d’Ivoire

Background: Some national and international strategies for the detection and prevention of emerging infectious diseases have been established across sectors. The capacity to carry out these tasks varies from country to country, and that remains largely undervalued. The Pasteur Institute of C?te d’Ivoire has created and implemented the capacity of national reference centers to fight against emerging and other infectious diseases. Objective: Show on the one hand the strategies used to develop the National Reference Centers and the IPCI and on the other hand the results obtained by performing these strategies. Method: Datas collection by documentary analysis (published scientific articles and grey literature) was done on Google Scholar, PUBMED and institutional reference documents. The documentary research was carried out to have a better understanding of strategies used to create and develop the NRCs in microbiology of communicable diseases. Results: Seven integrated strategies were launched: 1-training and workforce development;2-investigation of epidemics or public health events;3-strengthening laboratory epidemiological research;4-strengthening surveillance systems;5-improving communication with partners and stakeholders;6-building national and international collaborations;and 7-strengthening technical and technological platforms. In two decades, the number of researchers has risen from 10 in 2004 to ninety (90) in 2021, with 12 senior researchers and 32 junior researchers. A number of health service staff had attended a qualifying training course, 27 investigations into outbreaks and other public health events had been carried out, 18 short-term research projects had been launched, major surveillance programs and epidemiological research efforts on vector-borne, food-borne and nosocomial infections had begun, and several scientific manuscripts had been published or were edited in the writting press. Conclusion: The Ivorian experience shows that, with concerted effort, considerable progress can be made in the development and implementation of an infectious disease control program.

Study on the Gene Transcription Level of Hippocampus NGF in C57 Mouse Development

β-Nerve growth factor (β NGF) mRNA levels in hippocampa from C57BL/6J mice of different ages were compared by semi-quantitative RT-PCR method, taking β-actin gene as an internal control. The levels of 6-and 12-month-old C57 mice were much lower compared with that of the l-month-old mouse. However, there was no significant difference between these two groups. This result suggests that gene transcription level of hippocampus β NGF in a C57, mouse descends evidently as it grows up. Base T at Site 294 of β NGF cDNA was substituted by C in the C57, mouse, as revealed by DNA sequencing for the first time. Nevertheless, this polymorphism of nucleotide did not make any difference in amino acid composition.

陆地高分辨率水文—生物地球化学过程CNMM-DNDC三维模型的研发及应用进展

CNMM-DNDC模型是本文作者团队研发的陆地高分辨率水文—生物地球化学过程三维模型。本文系统介绍了建模背景和理念、核心过程和模型特点、模拟功能和观测验证、多尺度区域或流域初步应用以及未来发展展望。自2018年刊发首个版本以来,该模型经过了多方面科学过程改进和模拟功能扩展,在元素化学反应、物质相变和机械迁移等基本物理、化学、生物过程层面,完成了对陆地表层系统碳氮磷水循环全耦合的精细刻画。迄今开展的观测验证表明,CNMM-DNDC模型基本普适于不同生物气候带(从热带到寒区多年冻土地带)的流域或区域长时间序列“三高”(时间、空间和过程高分辨率)综合模拟,实现对陆地生态系统的碳、氮、磷、水三维运移、水土流失、水力驱动溶解态和颗粒态碳氮磷横向迁移、碳氮温室气体和污染气体排放、生态系统生产力、水分蒸散发和水分能量平衡等众多可持续发展目标表征变量的预测。该模型广泛推广应用于多尺度区域或流域的复杂过程虚拟科学试验研究和服务于面向生态环境建设与减污降碳的优化调控决策,可望为协同落实联合国多个可持续发展目标提供先进的数值模拟技术支撑。

在VC环境下运用Pro/Toolkit对齿轮标准件库的研究与开发

以Pro/E为开发平台,利用VC++6.0开发环境及Pro/Toolkit二次开发工具来进行齿轮参数化设计。运用Access 2002数据库来建立齿轮标准库CLBZK,然后通过ODBC数据库接口技术与MFC类库相连,从而可以利用Pro/Toolkit实现Pro/E对数据库的访问。