It has increasingly become a research focus to build higher structure composed of C60. However, there has been very few reports on the influence of polymer addition on the self-assembling behavior of fullerene in orga...It has increasingly become a research focus to build higher structure composed of C60. However, there has been very few reports on the influence of polymer addition on the self-assembling behavior of fullerene in organic solvents. In this research, big needle-like C60 assemblings have been obtained in the form of PLLA/C60 composites. The largest C60 needles can be observed by naked eyes. The amount of C60 in the composite influences the length of C60 needles to some extent. DSC results indicate C60 accelerates the crystallization and lift the relative crystallinity of PLLA matrix. the results also imply the addition of semicrystalline PLLA influence the assembling behavior of C60. i.e., the crystallization of PLLA accelerated by C60 also act a driving force for the enriching and the linear assembling of C60 in PLLA matrix via Van der Waals force.展开更多
AIM: To determine if calnexin(CANX), RAB1 and alphatubulin were involved in the production of hepatitis C virus(HCV) particles by baby hamster kidney-West Nile virus(BHK-WNV) cells. METHODS: Using a si RNA-based appro...AIM: To determine if calnexin(CANX), RAB1 and alphatubulin were involved in the production of hepatitis C virus(HCV) particles by baby hamster kidney-West Nile virus(BHK-WNV) cells. METHODS: Using a si RNA-based approach complemented with immuno-fluorescence confocal microscope and Western blot studies, we examined the roles of CANX, RAB1 and alpha-tubulin in the production of HCV particles by permissive BHK-WNV cells expressing HCV structural proteins or the full-length genome of HCV genotype 1a. Immuno-fluorescence studies in producer cells were performed with monoclonal antibodies against HCV structural proteins, as well as immunoglobulin from the serum of a patient recently cured from an HCV infection of same genotype. The cellular compartment stained by the serum immunoglobulin was also observedin thin section transmission electron microscopy. These findings were compared with the JFH-1 strain/Huh-7.5 cell model.RESULTS: We found that CANX was necessary for the production of HCV particles by BHK-WNV cells. This process involved the recruitment of a subset of HCV proteins, detected by immunoglobulin of an HCV-cured patient, in a compartment of rearranged membranes bypassing the endoplasmic reticulum-Golgi intermediary compartment and surrounded by mitochondria. It also involved the maturation of N-linked glycans on HCV envelope proteins, which was required for assembly and/or secretion of HCV particles. The formation of this specialized compartment required RAB1; upon expression of HCV structural genes, this compartment developed large vesicles with viral particles. RAB1 and alpha-tubulin were required for the release of HCV particles. These cellular factors were also involved in the production of HCVcc in the JFH-1 strain/Huh-7.5 cell system, which involves HCV RNA replication. The secretion of HCV particles by BHK-WNV cells presents similarities with a pathway involving caspase-1; a caspase-1 inhibitor was found to suppress the production of HCV particles from a full-length genome.CONCLUSION: Prior activity of the WNV subgenomic replicon in BHK-21 cells promoted re-wiring of host factors for the assembly and release of infectious HCV in a caspase-1-dependent mechanism.展开更多
Electrostatic layer-by-layer self-assembly multilayer films were successfully fabricated from C-60-ethylenediamine adduct (C-60-EDA) and DNA. Under visible light irradiation, DNA is ready to be cleaved and the films a...Electrostatic layer-by-layer self-assembly multilayer films were successfully fabricated from C-60-ethylenediamine adduct (C-60-EDA) and DNA. Under visible light irradiation, DNA is ready to be cleaved and the films are destroyed.展开更多
This is a further development work on grouping of printed circuit boards(PCBs)for Surface Mount Assembly in the electronic industry.The arrangement of PCBs among several surface mount machine lines is a typical kind o...This is a further development work on grouping of printed circuit boards(PCBs)for Surface Mount Assembly in the electronic industry.The arrangement of PCBs among several surface mount machine lines is a typical kind of group technology(GT)problem.From literatures,there are various clustering techniques developed to solve the clustering problems.In this paper,fuzzy c-means clustering(FCM)is used to solve the PCBs grouping problem.Applying them in a real problem compares the results of the two methods.The result shows that there should be a systematic method to arrange the scheduling of PCB assemblies in electronic industry to improve the operations planning process.展开更多
The 3D needled C/SiC brake materials modified with graphite were prepared by a combined process of the chemical vapor infiltration,slurry infiltration and liquid silicon infiltration process.The microstructure and fri...The 3D needled C/SiC brake materials modified with graphite were prepared by a combined process of the chemical vapor infiltration,slurry infiltration and liquid silicon infiltration process.The microstructure and frictional properties of the brake materials were investigated.The density and open porosity of the materials as-received were about(2.1±0.1)g/cm3and(5±1)%,respectively.The brake materials were composed of 59%C,39%SiC,and 2%Si(mass fraction).The content of Si in the C/SiC brake materials modified with graphite was far less than that in the C/SiC brake materials without being modified with graphite,and the Si was dispersed.The braking curve of the 3D needled C/SiC modified with graphite was smooth,which can ensure the smooth and comfortable braking.The frictional properties under wet condition of the 3D needled C/SiC modified with graphite showed no fading.And the linear wear rate of the C/SiC modified with graphite was lower than that of the C/SiC unmodified.展开更多
The C/C composite brake discs were prepared by tri-cylindrical chemical vapor deposition （CVD） process. The optimum processing parameters were as follows： deposition temperature was 830 - 930 ℃, the gas- flow rate...The C/C composite brake discs were prepared by tri-cylindrical chemical vapor deposition （CVD） process. The optimum processing parameters were as follows： deposition temperature was 830 - 930 ℃, the gas- flow rates of N2 and propylene were 4.8 - 5.2 m^3/h and 5.8 - 6.2 m^3/h, respectively, the furnace pressure was 4.5 - 5.5 kPa and the deposition time was 200 h. The effects of processing parameters on the densified rates, thermal-physical property and mechanical performance of C/C composite brake discs were studied. The results show that density, heat conductivity, bend strength and abrasion ratio of the multi-cylindrica brake discs are 1. 02 - 1. 78 g/cm^3 , 31 W/（m·K）, 114 MPa and 7μm/s, respectively, which are approximately similar to those of the singlecylindrical ones. The gas tlow rate has no relation to the number of the cylinder and furnace loading. The utilization ratio of carbon can be improved by multi-cylinder CVD process without changing the characteristics of brake disc.展开更多
An initial investigation on the roughness and frictional properties of the self-assembled thin films from polyelectrolytes is presented. Star-shaped C-60-Poly(styrene-maleic anhydride) was successful prepared. The mul...An initial investigation on the roughness and frictional properties of the self-assembled thin films from polyelectrolytes is presented. Star-shaped C-60-Poly(styrene-maleic anhydride) was successful prepared. The multilayer thin films have been fabricated on mica with diazoresin as the cationic polyelectrolyte and hydrolyzed star-shaped C-60-poly(styrene-maleic anhydride) as the anionic polyelectrolyte via self-assembly technique. The crosslinking structure of the films is formed from the conversion of ionic bond to covalent bond after UV irradiation. AFM/FFM investigations provide insights into the roughness and frictional properties on a microscale. The roughness depends strongly on the number of film layers in the case of C-60-containing films. The frictional forces of the films exhibited a well behaved non-linear relationship in response to the change of applied load. It supports the prediction of enhanced load-bearing property Of C60-containing thin films.展开更多
A new self-assembled monolayer (SAM) of fullerene derivative has been fabricated and their photoelectric conversion property has been observed. The SAM was fabricated on indium-tin-oxide (ITO) and quartz by amide bond...A new self-assembled monolayer (SAM) of fullerene derivative has been fabricated and their photoelectric conversion property has been observed. The SAM was fabricated on indium-tin-oxide (ITO) and quartz by amide bonding. It was characterized bq contact angle, UV spectrum. and cyclic voltammetry. The mild fabrication conditions and the stability of the SAM provides a way to build three-dimensional structure more easily.展开更多
Quartz crystal microbalance（QCM） and cyclic voltammetry（CV） were used to characterize the monolayer of cytochrome c（Cyt c）,which was adsorbed on gold film modified with alkanethiol mixed monolayer.A direct compa...Quartz crystal microbalance（QCM） and cyclic voltammetry（CV） were used to characterize the monolayer of cytochrome c（Cyt c）,which was adsorbed on gold film modified with alkanethiol mixed monolayer.A direct comparison of protein surface coverages calculated from QCM and cyclic voltammetric measurements illustrates that the ratio of the electroactive Cyt c to the total surface-confined Cyt c is 34%,which suggests that the orientation is a main factor affecting the electroactivity of Cyt c.Moreover,surface plasmon resonance（SPR） measurement combined with CV ＂in situ＂ was used to investigate the conformational change of Cyt c in the redox process.Besides,Au nanoparticles（Au NPs） were adsorbed on the surface of Cyt c.The result indicates that Au NPs promote electron transfer between Cyt c and the gold electrode,and SPR result suggests Au NPs enhance SPR signal.展开更多
文摘It has increasingly become a research focus to build higher structure composed of C60. However, there has been very few reports on the influence of polymer addition on the self-assembling behavior of fullerene in organic solvents. In this research, big needle-like C60 assemblings have been obtained in the form of PLLA/C60 composites. The largest C60 needles can be observed by naked eyes. The amount of C60 in the composite influences the length of C60 needles to some extent. DSC results indicate C60 accelerates the crystallization and lift the relative crystallinity of PLLA matrix. the results also imply the addition of semicrystalline PLLA influence the assembling behavior of C60. i.e., the crystallization of PLLA accelerated by C60 also act a driving force for the enriching and the linear assembling of C60 in PLLA matrix via Van der Waals force.
基金Supported by Intramural Program of the National Institutes of Health,National Institute of Allergy and Infectious Diseases(Project No.1 ZIA AI000733-15:Enveloped Virus Glycoprotein/Receptor Interactions)to Edward A Berger,PhD(MSS,LVD,DIR,NIAID)ORISE Senior Fellow award(Award No.1238-1238-03:Department of Energy/Oak Ridge Institute for Science and Education)to Bertrand Saunier,MD,PhD
文摘AIM: To determine if calnexin(CANX), RAB1 and alphatubulin were involved in the production of hepatitis C virus(HCV) particles by baby hamster kidney-West Nile virus(BHK-WNV) cells. METHODS: Using a si RNA-based approach complemented with immuno-fluorescence confocal microscope and Western blot studies, we examined the roles of CANX, RAB1 and alpha-tubulin in the production of HCV particles by permissive BHK-WNV cells expressing HCV structural proteins or the full-length genome of HCV genotype 1a. Immuno-fluorescence studies in producer cells were performed with monoclonal antibodies against HCV structural proteins, as well as immunoglobulin from the serum of a patient recently cured from an HCV infection of same genotype. The cellular compartment stained by the serum immunoglobulin was also observedin thin section transmission electron microscopy. These findings were compared with the JFH-1 strain/Huh-7.5 cell model.RESULTS: We found that CANX was necessary for the production of HCV particles by BHK-WNV cells. This process involved the recruitment of a subset of HCV proteins, detected by immunoglobulin of an HCV-cured patient, in a compartment of rearranged membranes bypassing the endoplasmic reticulum-Golgi intermediary compartment and surrounded by mitochondria. It also involved the maturation of N-linked glycans on HCV envelope proteins, which was required for assembly and/or secretion of HCV particles. The formation of this specialized compartment required RAB1; upon expression of HCV structural genes, this compartment developed large vesicles with viral particles. RAB1 and alpha-tubulin were required for the release of HCV particles. These cellular factors were also involved in the production of HCVcc in the JFH-1 strain/Huh-7.5 cell system, which involves HCV RNA replication. The secretion of HCV particles by BHK-WNV cells presents similarities with a pathway involving caspase-1; a caspase-1 inhibitor was found to suppress the production of HCV particles from a full-length genome.CONCLUSION: Prior activity of the WNV subgenomic replicon in BHK-21 cells promoted re-wiring of host factors for the assembly and release of infectious HCV in a caspase-1-dependent mechanism.
基金The National Natural Science Foundation of China (Grant No. 29774036 and 29904007) and PPLAS Foundation of Chinese Academy of Sciences (Grant No. 01-B-06) are gratefully acknowledged for their financial support of this work.
文摘Electrostatic layer-by-layer self-assembly multilayer films were successfully fabricated from C-60-ethylenediamine adduct (C-60-EDA) and DNA. Under visible light irradiation, DNA is ready to be cleaved and the films are destroyed.
文摘This is a further development work on grouping of printed circuit boards(PCBs)for Surface Mount Assembly in the electronic industry.The arrangement of PCBs among several surface mount machine lines is a typical kind of group technology(GT)problem.From literatures,there are various clustering techniques developed to solve the clustering problems.In this paper,fuzzy c-means clustering(FCM)is used to solve the PCBs grouping problem.Applying them in a real problem compares the results of the two methods.The result shows that there should be a systematic method to arrange the scheduling of PCB assemblies in electronic industry to improve the operations planning process.
基金Project(46-QP-2009)supported by the Research Fund of State Key Laboratory of Solidification Processing(NWPU),ChinaProject supported by the Program for Changjiang Scholars and Innovative Research Team in Chinese University
文摘The 3D needled C/SiC brake materials modified with graphite were prepared by a combined process of the chemical vapor infiltration,slurry infiltration and liquid silicon infiltration process.The microstructure and frictional properties of the brake materials were investigated.The density and open porosity of the materials as-received were about(2.1±0.1)g/cm3and(5±1)%,respectively.The brake materials were composed of 59%C,39%SiC,and 2%Si(mass fraction).The content of Si in the C/SiC brake materials modified with graphite was far less than that in the C/SiC brake materials without being modified with graphite,and the Si was dispersed.The braking curve of the 3D needled C/SiC modified with graphite was smooth,which can ensure the smooth and comfortable braking.The frictional properties under wet condition of the 3D needled C/SiC modified with graphite showed no fading.And the linear wear rate of the C/SiC modified with graphite was lower than that of the C/SiC unmodified.
文摘The C/C composite brake discs were prepared by tri-cylindrical chemical vapor deposition （CVD） process. The optimum processing parameters were as follows： deposition temperature was 830 - 930 ℃, the gas- flow rates of N2 and propylene were 4.8 - 5.2 m^3/h and 5.8 - 6.2 m^3/h, respectively, the furnace pressure was 4.5 - 5.5 kPa and the deposition time was 200 h. The effects of processing parameters on the densified rates, thermal-physical property and mechanical performance of C/C composite brake discs were studied. The results show that density, heat conductivity, bend strength and abrasion ratio of the multi-cylindrica brake discs are 1. 02 - 1. 78 g/cm^3 , 31 W/（m·K）, 114 MPa and 7μm/s, respectively, which are approximately similar to those of the singlecylindrical ones. The gas tlow rate has no relation to the number of the cylinder and furnace loading. The utilization ratio of carbon can be improved by multi-cylinder CVD process without changing the characteristics of brake disc.
基金This project is financially supported by the National Natural Science Foundation of China (No. 59843008).
文摘An initial investigation on the roughness and frictional properties of the self-assembled thin films from polyelectrolytes is presented. Star-shaped C-60-Poly(styrene-maleic anhydride) was successful prepared. The multilayer thin films have been fabricated on mica with diazoresin as the cationic polyelectrolyte and hydrolyzed star-shaped C-60-poly(styrene-maleic anhydride) as the anionic polyelectrolyte via self-assembly technique. The crosslinking structure of the films is formed from the conversion of ionic bond to covalent bond after UV irradiation. AFM/FFM investigations provide insights into the roughness and frictional properties on a microscale. The roughness depends strongly on the number of film layers in the case of C-60-containing films. The frictional forces of the films exhibited a well behaved non-linear relationship in response to the change of applied load. It supports the prediction of enhanced load-bearing property Of C60-containing thin films.
文摘A new self-assembled monolayer (SAM) of fullerene derivative has been fabricated and their photoelectric conversion property has been observed. The SAM was fabricated on indium-tin-oxide (ITO) and quartz by amide bonding. It was characterized bq contact angle, UV spectrum. and cyclic voltammetry. The mild fabrication conditions and the stability of the SAM provides a way to build three-dimensional structure more easily.
基金Supported by the National Natural Science Foundation of China(Nos.20833005,20873116,60936003 and 21021002)the Fund of the State Key Laboratory of Superamolecular Structure and Materials,Jilin University,China(No.200910)
文摘Quartz crystal microbalance（QCM） and cyclic voltammetry（CV） were used to characterize the monolayer of cytochrome c（Cyt c）,which was adsorbed on gold film modified with alkanethiol mixed monolayer.A direct comparison of protein surface coverages calculated from QCM and cyclic voltammetric measurements illustrates that the ratio of the electroactive Cyt c to the total surface-confined Cyt c is 34%,which suggests that the orientation is a main factor affecting the electroactivity of Cyt c.Moreover,surface plasmon resonance（SPR） measurement combined with CV ＂in situ＂ was used to investigate the conformational change of Cyt c in the redox process.Besides,Au nanoparticles（Au NPs） were adsorbed on the surface of Cyt c.The result indicates that Au NPs promote electron transfer between Cyt c and the gold electrode,and SPR result suggests Au NPs enhance SPR signal.
