In view of the composition analysis and identification of ancient glass products, L1 regularization, K-Means cluster analysis, elbow rule and other methods were comprehensively used to build logical regression, cluste...In view of the composition analysis and identification of ancient glass products, L1 regularization, K-Means cluster analysis, elbow rule and other methods were comprehensively used to build logical regression, cluster analysis, hyper-parameter test and other models, and SPSS, Python and other tools were used to obtain the classification rules of glass products under different fluxes, sub classification under different chemical compositions, hyper-parameter K value test and rationality analysis. Research can provide theoretical support for the protection and restoration of ancient glass relics.展开更多
The properties of generalized flip Markov chains on connected regular digraphs are discussed.The 1-Flipper operation on Markov chains for undirected graphs is generalized to that for multi-digraphs.The generalized 1-F...The properties of generalized flip Markov chains on connected regular digraphs are discussed.The 1-Flipper operation on Markov chains for undirected graphs is generalized to that for multi-digraphs.The generalized 1-Flipper operation preserves the regularity and weak connectivity of multi-digraphs.The generalized 1-Flipper operation is proved to be symmetric.Moreover,it is presented that a series of random generalized 1-Flipper operations eventually lead to a uniform probability distribution over all connected d-regular multi-digraphs without loops.展开更多
Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2)are key transcription factors that regulate a complex gene network in the liver,cre-ating a regulator...Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2)are key transcription factors that regulate a complex gene network in the liver,cre-ating a regulatory transcriptional loop.The Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase genes.Our in silico analysis of HNF1A,HNF4A.and FOXA2 binding to the ten candidate glyco-genes studied in this work confirms a significant enrich-ment of these transcription factors specifically in the liver.Our previous studies identified HNF1A as a master regulator of fucosylation,glycan branching,and galactosylation of plasma glycoproteins.Here,we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan phenotype.We used the state-of-the-art clus-tered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9)molecular tool for the downregulation of the HNF1A,HNF4A,and FOXA2 genes in HepG2 cells-a human liver cancer cell line.The results show that the downregulation of all three genes individually and in pairs affects the transcrip-tional activity of many glyco-genes,although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan structures.The effect is better seen as an overall change in the total HepG2 N-glycome,primarily due to the extension of biantennary glycans.We propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan structure.We also propose a model showing feedback loops with the mutual activation of HNF1A-FOXA2 and HNF4A-FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.展开更多
Let us consider the following elliptic systems of second order-D_α(A_i~α(x, u, Du))=B_4(x, u, Du), i=1, …, N, x∈Q(?)R^n, n≥3 (1) and supposeⅰ) |A_i~α(x, u, Du)|≤L(1+|Du|);ⅱ) (1+|p|)^(-1)A_i~α(x, u, p)are H(?...Let us consider the following elliptic systems of second order-D_α(A_i~α(x, u, Du))=B_4(x, u, Du), i=1, …, N, x∈Q(?)R^n, n≥3 (1) and supposeⅰ) |A_i~α(x, u, Du)|≤L(1+|Du|);ⅱ) (1+|p|)^(-1)A_i~α(x, u, p)are H(?)lder-continuous functions with some exponent δ on (?)×R^N uniformly with respect to p, i.e.ⅲ) A_i~α(x, u, p) are differentiable function in p with bounded and continuous derivativesⅳ)ⅴ) for all u∈H_(loc)~1(Ω, R^N)∩L^(n(γ-1)/(2-γ))(Ω, R^N), B(x, u, Du)is ineasurable and |B(x, u, p)|≤a(|p|~γ+|u|~τ)+b(x), where 1+2/n<γ<2, τ≤max((n+2)/(n-2), (γ-1)/(2-γ)-ε), (?)ε>0, b(x)∈L2n/(n+2), n^2/(n+2)+e(Ω), (?)ε>0.Remarks. Only bounded open set Q will be considered in this paper; for all p≥1, λ≥0, which is clled a Morrey Space.Let assumptions ⅰ)-ⅳ) hold, Giaquinta and Modica have proved the regularity of both the H^1 weak solutions of (1) under controllable growth condition |B|≤α(|p|~γ+|u|^((n+2)/(n-2))+b, 0<γ≤1+2/n and the H^1∩L~∞ weak solutions of (1) under natural growth condition |B|≤α|p|~2+b with a smallness condition 2aM<λ(|u|≤M), which implys that the H^1∩L~∞ weak solutions have the same regularty in the case of 1+2/n<γ<2. In the case of γ=2, many counterexamples (see [2] showed that u must be in H^1L~∞, while in the case of 1+2/n<γ<2, we consider the H^1∩L^n(γ-1)/(2-γ) weak solutions of (1), weaken the instability conditions upon them (from L~∞ to L^n(γ-1)/(2-γ) and obtain the same regularity results. Finally we show that the exponent n(γ-1)/(2-γ) can not be docreased anymore for the sake of the regularity results.Delinition 1. We call u∈H^1∩L^n(γ-1)/(2-γ)(Q, R^N) be a weak solution of (1), providod that where We use the convention that repeated indices are summed. i, j go from 1 to N ann α, β from 1 to n.展开更多
将新近提出的C0有限元后处理中超收敛解答计算的单元能量投影(Element Energy Projection,简称EEP)法推广到一维C1类有限元。根据单元投影定理具体推导了一般梁单元的计算公式,并对两个有代表性的单元给出了数值算例。分析和算例表明,EE...将新近提出的C0有限元后处理中超收敛解答计算的单元能量投影(Element Energy Projection,简称EEP)法推广到一维C1类有限元。根据单元投影定理具体推导了一般梁单元的计算公式,并对两个有代表性的单元给出了数值算例。分析和算例表明,EEP法在一维C1类有限元中再次获得令人满意的效果,即对任一单元中的任一点,从位移一直到三阶导数(如梁的挠度、转角、弯矩、剪力),匀可获得与结点位移精度相当的超收敛结果,而且可精确满足自然边界条件。展开更多
文摘In view of the composition analysis and identification of ancient glass products, L1 regularization, K-Means cluster analysis, elbow rule and other methods were comprehensively used to build logical regression, cluster analysis, hyper-parameter test and other models, and SPSS, Python and other tools were used to obtain the classification rules of glass products under different fluxes, sub classification under different chemical compositions, hyper-parameter K value test and rationality analysis. Research can provide theoretical support for the protection and restoration of ancient glass relics.
基金National Natural Science Foundation of China(No.11671258)。
文摘The properties of generalized flip Markov chains on connected regular digraphs are discussed.The 1-Flipper operation on Markov chains for undirected graphs is generalized to that for multi-digraphs.The generalized 1-Flipper operation preserves the regularity and weak connectivity of multi-digraphs.The generalized 1-Flipper operation is proved to be symmetric.Moreover,it is presented that a series of random generalized 1-Flipper operations eventually lead to a uniform probability distribution over all connected d-regular multi-digraphs without loops.
基金the European Structural and Investment Funded Grant"Cardio Metabolic"(#KK.01.2.1.02.0321)the Croatian National Centre of Research Excellence in Personalized Healthcare Grant(#KK.01.1.1.01.0010)+2 种基金the European Regional Development Fund Grant,project"CRISPR/Cas9-CasMouse"(#KK.01.1.1.04.0085)the European Structural and Investment Funded Project of Centre of Competence in Molecular Diagnostics(#KK.01.2.2.03.0006)the Croatian National Centre of Research Excellence in Personalized Healthcare Grant(#KK.01.1.1.01.0010).
文摘Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2)are key transcription factors that regulate a complex gene network in the liver,cre-ating a regulatory transcriptional loop.The Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase genes.Our in silico analysis of HNF1A,HNF4A.and FOXA2 binding to the ten candidate glyco-genes studied in this work confirms a significant enrich-ment of these transcription factors specifically in the liver.Our previous studies identified HNF1A as a master regulator of fucosylation,glycan branching,and galactosylation of plasma glycoproteins.Here,we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan phenotype.We used the state-of-the-art clus-tered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9)molecular tool for the downregulation of the HNF1A,HNF4A,and FOXA2 genes in HepG2 cells-a human liver cancer cell line.The results show that the downregulation of all three genes individually and in pairs affects the transcrip-tional activity of many glyco-genes,although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan structures.The effect is better seen as an overall change in the total HepG2 N-glycome,primarily due to the extension of biantennary glycans.We propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan structure.We also propose a model showing feedback loops with the mutual activation of HNF1A-FOXA2 and HNF4A-FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.
基金This work is supported in part by the Foundation of Zhongshan University, Advanced Research Center.
文摘Let us consider the following elliptic systems of second order-D_α(A_i~α(x, u, Du))=B_4(x, u, Du), i=1, …, N, x∈Q(?)R^n, n≥3 (1) and supposeⅰ) |A_i~α(x, u, Du)|≤L(1+|Du|);ⅱ) (1+|p|)^(-1)A_i~α(x, u, p)are H(?)lder-continuous functions with some exponent δ on (?)×R^N uniformly with respect to p, i.e.ⅲ) A_i~α(x, u, p) are differentiable function in p with bounded and continuous derivativesⅳ)ⅴ) for all u∈H_(loc)~1(Ω, R^N)∩L^(n(γ-1)/(2-γ))(Ω, R^N), B(x, u, Du)is ineasurable and |B(x, u, p)|≤a(|p|~γ+|u|~τ)+b(x), where 1+2/n<γ<2, τ≤max((n+2)/(n-2), (γ-1)/(2-γ)-ε), (?)ε>0, b(x)∈L2n/(n+2), n^2/(n+2)+e(Ω), (?)ε>0.Remarks. Only bounded open set Q will be considered in this paper; for all p≥1, λ≥0, which is clled a Morrey Space.Let assumptions ⅰ)-ⅳ) hold, Giaquinta and Modica have proved the regularity of both the H^1 weak solutions of (1) under controllable growth condition |B|≤α(|p|~γ+|u|^((n+2)/(n-2))+b, 0<γ≤1+2/n and the H^1∩L~∞ weak solutions of (1) under natural growth condition |B|≤α|p|~2+b with a smallness condition 2aM<λ(|u|≤M), which implys that the H^1∩L~∞ weak solutions have the same regularty in the case of 1+2/n<γ<2. In the case of γ=2, many counterexamples (see [2] showed that u must be in H^1L~∞, while in the case of 1+2/n<γ<2, we consider the H^1∩L^n(γ-1)/(2-γ) weak solutions of (1), weaken the instability conditions upon them (from L~∞ to L^n(γ-1)/(2-γ) and obtain the same regularity results. Finally we show that the exponent n(γ-1)/(2-γ) can not be docreased anymore for the sake of the regularity results.Delinition 1. We call u∈H^1∩L^n(γ-1)/(2-γ)(Q, R^N) be a weak solution of (1), providod that where We use the convention that repeated indices are summed. i, j go from 1 to N ann α, β from 1 to n.
文摘将新近提出的C0有限元后处理中超收敛解答计算的单元能量投影(Element Energy Projection,简称EEP)法推广到一维C1类有限元。根据单元投影定理具体推导了一般梁单元的计算公式,并对两个有代表性的单元给出了数值算例。分析和算例表明,EEP法在一维C1类有限元中再次获得令人满意的效果,即对任一单元中的任一点,从位移一直到三阶导数(如梁的挠度、转角、弯矩、剪力),匀可获得与结点位移精度相当的超收敛结果,而且可精确满足自然边界条件。
