The multifunctional trans-activator Tat is an essential regulatory protein for HIV-1 replication and is characterized by high sequence diversity. Numerous experimental studies have examined Tat in HIV-1 subtype B, but...The multifunctional trans-activator Tat is an essential regulatory protein for HIV-1 replication and is characterized by high sequence diversity. Numerous experimental studies have examined Tat in HIV-1 subtype B, but research on subtype C Tat is lacking, despite the high prevalence of infections caused by subtype C worldwide. We hypothesized that amino acid differences contribute to functional differences among Tat proteins. In the present study, we found that subtype B NL4-3Tat and subtype C isolate HIV1084 i Tat exhibited differences in stability by overexpressing the fusion protein Tat-Flag. In addition, 1084 i Tat can activate LTR and NF-κB more efficiently than NL4-3 Tat. In analyses of the activities of the truncated forms of Tat, we found that the carboxylterminal region of Tat regulates its stability and transactivity. According to our results, we speculated that the differences in stability between B-Tat and C-Tat result in differences in transactivation ability.展开更多
The authors consider a family of finite-dimensional Lie superalgebras of C-type over an algebraically closed field of characteristic p 〉 3. It is proved that the Lie superalgebras of C-type are simple and the spannin...The authors consider a family of finite-dimensional Lie superalgebras of C-type over an algebraically closed field of characteristic p 〉 3. It is proved that the Lie superalgebras of C-type are simple and the spanning sets are determined. Then the spanning sets are employed to characterize the superderivation algebras of these Lie superalgebras. Finally, the associative forms are discussed and a comparison is made between these Lie superalgebras and other simple Lie superalgebras of Cartan type.展开更多
基金supported by grants from the National Natural Science Foundation of China (No.81571987 and 81371820)the Ph.D. Candidate Research Innovation Fund of Nankai University (2015) (No.68150003)
文摘The multifunctional trans-activator Tat is an essential regulatory protein for HIV-1 replication and is characterized by high sequence diversity. Numerous experimental studies have examined Tat in HIV-1 subtype B, but research on subtype C Tat is lacking, despite the high prevalence of infections caused by subtype C worldwide. We hypothesized that amino acid differences contribute to functional differences among Tat proteins. In the present study, we found that subtype B NL4-3Tat and subtype C isolate HIV1084 i Tat exhibited differences in stability by overexpressing the fusion protein Tat-Flag. In addition, 1084 i Tat can activate LTR and NF-κB more efficiently than NL4-3 Tat. In analyses of the activities of the truncated forms of Tat, we found that the carboxylterminal region of Tat regulates its stability and transactivity. According to our results, we speculated that the differences in stability between B-Tat and C-Tat result in differences in transactivation ability.
基金supported by the National Natural Science Foundation of China(No.11371182)the PhD Start-up Foundation of Liaoning University of China(No.2012002)the Predeclaration Fund of State Project of Liaoning University(No.2014LDGY01)
文摘The authors consider a family of finite-dimensional Lie superalgebras of C-type over an algebraically closed field of characteristic p 〉 3. It is proved that the Lie superalgebras of C-type are simple and the spanning sets are determined. Then the spanning sets are employed to characterize the superderivation algebras of these Lie superalgebras. Finally, the associative forms are discussed and a comparison is made between these Lie superalgebras and other simple Lie superalgebras of Cartan type.
