AIM To evaluate the numbers of different subsets of monocytes and their associations with the values of clinical measures in mild acute pancreatitis(MAP) patients.METHODS The study included one group of 13 healthy con...AIM To evaluate the numbers of different subsets of monocytes and their associations with the values of clinical measures in mild acute pancreatitis(MAP) patients.METHODS The study included one group of 13 healthy controls and another group of 24 patients with new-onset MAP. The numbers of different subsets of monocytes were examined in these two groups of subjects by flow cytometry. The concentrations of plasma interleukin(IL)-10 and IL-12 were determined by cytometric bead array. The acute physiology and chronic health evaluation(APACHE) II scores of individual patients were evaluated, and the levels of plasma C-reactive protein(CRP) as well as the activities of amylase and lipase were measured. RESULTS In comparison with that in the controls, significantly increased numbers of CD14+CD163-, CD14+CD163-MAC387+ M1 monocytes, but significantly reduced numbers of CD14+CD163+IL-10+ M2 monocytes were detected in the MAP patients(P < 0.01 or P < 0.05). Furthermore, significantly higher levels of plasma IL-10 and IL-12 were observed in the MAP patients(P < 0.01 for all). More importantly, the levels of plasma CRP were positively correlated with the numbers of CD14+CD163-(R = 0.5009, P = 0.0127) and CD14+CD163-MAC387+(R = 0.5079, P = 0.0113) M1 monocytes and CD14+CD163+CD115+ M2 monocytes(R = 0.4565, P = 0.0249) in the patients. The APACHE II scores correlated with the numbers of CD14+CD163+CD115+(R = 0.4581, P = 0.0244) monocytes and the levels of plasma IL-10(R = 0.4178, P = 0.0422) in the MAP patients. However, there was no significant association among other measures tested in this population. CONCLUSION Increased numbers of CD14+CD163- and CD14+ CD163-MAC387+ monocytes may contribute to the pathogenesis of MAP, and increased numbers of CD14+CD163+CD115+ monocytes may be a biomarker for evaluating the severity of MAP.展开更多
文摘AIM To evaluate the numbers of different subsets of monocytes and their associations with the values of clinical measures in mild acute pancreatitis(MAP) patients.METHODS The study included one group of 13 healthy controls and another group of 24 patients with new-onset MAP. The numbers of different subsets of monocytes were examined in these two groups of subjects by flow cytometry. The concentrations of plasma interleukin(IL)-10 and IL-12 were determined by cytometric bead array. The acute physiology and chronic health evaluation(APACHE) II scores of individual patients were evaluated, and the levels of plasma C-reactive protein(CRP) as well as the activities of amylase and lipase were measured. RESULTS In comparison with that in the controls, significantly increased numbers of CD14+CD163-, CD14+CD163-MAC387+ M1 monocytes, but significantly reduced numbers of CD14+CD163+IL-10+ M2 monocytes were detected in the MAP patients(P < 0.01 or P < 0.05). Furthermore, significantly higher levels of plasma IL-10 and IL-12 were observed in the MAP patients(P < 0.01 for all). More importantly, the levels of plasma CRP were positively correlated with the numbers of CD14+CD163-(R = 0.5009, P = 0.0127) and CD14+CD163-MAC387+(R = 0.5079, P = 0.0113) M1 monocytes and CD14+CD163+CD115+ M2 monocytes(R = 0.4565, P = 0.0249) in the patients. The APACHE II scores correlated with the numbers of CD14+CD163+CD115+(R = 0.4581, P = 0.0244) monocytes and the levels of plasma IL-10(R = 0.4178, P = 0.0422) in the MAP patients. However, there was no significant association among other measures tested in this population. CONCLUSION Increased numbers of CD14+CD163- and CD14+ CD163-MAC387+ monocytes may contribute to the pathogenesis of MAP, and increased numbers of CD14+CD163+CD115+ monocytes may be a biomarker for evaluating the severity of MAP.
文摘目的探讨关节镜滑膜切除术(arthroscopic synovectomy,AS)对类风湿关节炎(rheumatoid arthritis,RA)患者疾病活动的影响,分析术后RA疾病活动相关因素。方法回顾性选取2018年1月至2019年1月期间行单次AS的105例RA患者作为研究对象,男17例,女88例(83.8%);年龄58~76岁,平均(67.57±9.09)岁;病程(17.93±12.52)年。手术部位:肘关节10例(9.5%),膝关节73例(69.5%),踝关节22例(21%)。采用双抗体夹心ELISA法检测血C反应蛋白(C-reactive protein,CRP),应用基于C反应蛋白的28关节疾病活动度评分(disease activity score in 28 joints based on C-reactive protein,DAS28-CRP)的评估RA疾病活动,采用SPSS 23.0统计学软件对数据进行统计分析,并用多因素Logistic回归分析术后RA疾病活动相关因素。结果105例均获随访,随访时间10~12个月,平均(11.2±5.9)个月。5例出现术后并发症,其中3例手术关节功能受限,2例关节内血肿;3例患者术后关节疼痛未缓解。术后患者DAS28-CRP及CRP与基线时比较,差异有统计学意义(P<0.05)。Pearson相关分析结果提示ΔDAS28-CRP与病程呈正相关,与基线疾病活动度及大关节手术呈负相关(r值分别为0.265,-0.592,-0.395,P<0.05)。多因素logistic回归分析显示,较短病程OR=2.781,95%CI(1.011,6.111),大关节手术OR=0.158,95%CI(0.101,0.251),较高的基线DAS28-CRP评分OR=0.098,95%CI(0.035,0.175),具有统计学意义(P<0.05),是术后疾病活动降低的独立相关因素。结论病程较短、疾病活动度较高且药物治疗效果欠佳的RA患者,行大关节的AS可能显著改善疾病活动。
