Partition-based clustering with weighted feature is developed in the framework of shadowed sets. The objects in the core and boundary regions, generated by shadowed sets-based clustering, have different impact on the ...Partition-based clustering with weighted feature is developed in the framework of shadowed sets. The objects in the core and boundary regions, generated by shadowed sets-based clustering, have different impact on the prototype of each cluster. By integrating feature weights, a formula for weight calculation is introduced to the clustering algorithm. The selection of weight exponent is crucial for good result and the weights are updated iteratively with each partition of clusters. The convergence of the weighted algorithms is given, and the feasible cluster validity indices of data mining application are utilized. Experimental results on both synthetic and real-life numerical data with different feature weights demonstrate that the weighted algorithm is better than the other unweighted algorithms.展开更多
Total mRNA was extracted from a venom gland of snake Daboia russellii siamensis following the manufacturer's protocol of the PolyATtract System 1000 kit purchased from Promega Biotech. cDNAs encoding C-type lectins w...Total mRNA was extracted from a venom gland of snake Daboia russellii siamensis following the manufacturer's protocol of the PolyATtract System 1000 kit purchased from Promega Biotech. cDNAs encoding C-type lectins were amplified by RT-PCR and subcloned into a pMD18-T vector. Fourteen positive clones were selected for nucleotide sequencing and seven cDNAs encoding various snake venom C-type lectin-like protein precursors, designated as DRS-L1, DRS-L2, DRS-L3, DRS-L4 DRS-L5, DRS-L6 and DRS-L7, were obtained. Amino acid sequences of these proteins were deduced and each contains a carbohydrate recognition domain. Of all the deduced protein sequences, only DRS-L1 seemed to represent a closer sequence similarity to α subunits of other known snake venom C-type lectin-like proteins using the BLAST program. Homology comparison combined with analysis of cysteine position indicate that DRS-L1 and DRS-L2 are probably the light chain LC2 and LC1 of factor X activator from Daboia russellii siamensis venom, respectively. DRS-L3 and DRS-L4 might be the β subunits of higher molecular weight C-type lectin-like proteins while DRS-L5 and DRS-L6 might be β subunits of lower molecular weight C-type lectin-like proteins. DRS-L7 might be the β subunit of a platelet membrane glycoprotein Ib-binding protein similar to echicetin.展开更多
Acting as one of the pattern recognition receptors (PRRs), C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In ...Acting as one of the pattern recognition receptors (PRRs), C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene (named LvLecl) was cloned from the shrimp Litopenaeus vannamei, The ORF of LvLecl is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain (CRD) at the C-terminal. LvLecl was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLecl transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS, Micrococcus lysodeikticus and white spot syndrome virus (WSSV). RNAi-based silencing of LvLecl resulted in increases in mortality when the shrimp were challenged with WSSV, and the median lethal time was reduced compared with controls. Although there was no characteristic "EPN" (Glu-Pro-Ser) or "QPD" (Gin-Pro-Asp) motif, the recombinant LvLecl, expressed in Escherichia coli BL21 (DE3), could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose, D-glucose, D-galactose and N-Acetyl-D-mannose. These results suggest that LvLecl might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei.展开更多
A seroprevalence investigation of human brucellosis was carried out in Kuku Dairy Scheme, Sudan. A total of 176 serum samples were collected and screened by Rose Bengal Plate Test (RBPT). The positive sera vqere fur...A seroprevalence investigation of human brucellosis was carried out in Kuku Dairy Scheme, Sudan. A total of 176 serum samples were collected and screened by Rose Bengal Plate Test (RBPT). The positive sera vqere further examined using Tube Agglutination Test (TAT) and c-Elisa. The seropositivity was 15.9%, 14.8% and 11.4% using RBPT, TAT and c-Elisa respectively. Whereas, the active infection based on seropositivity and clinical signs were 4.6%, 4.6% and 2.3% in case of RBPT, TAT and c-Elisa respectively. Based on c-Elisa result the infected individuals were further subjected to clinical examination and treated with streptomycin and doxocycline for six weeks.展开更多
Lectins play a crucial role in the innate immunity of invertebrates and vertebrates by recognizing and disposing of pathogens. We obtained the complete cDNA of a C-type lectin (EALecl) from Epinephelus akaara using ...Lectins play a crucial role in the innate immunity of invertebrates and vertebrates by recognizing and disposing of pathogens. We obtained the complete cDNA of a C-type lectin (EALecl) from Epinephelus akaara using RACE. The complete EALecl cDNA sequence was 827 bp. The 5-UTR and 3-UTR were 28 bp and 151 bp, respectively, in length. The sequence also contained a polyadenylation signal AATAAA and a poly(A) tail. The EALecl cDNA encodes polypeptides with 215 amino acids, including a signal peptide of 31 amino acids. The protein has a cysteine-rich region at the N terminal, a collagenous region characterized by G-X-Y repeats, a neck region, and a typical carbohydrate-recognition domain (CRD), indicating that EALecl is a collectin. The key recognition positions of this CRD are EPD, isolated for the first time in fish. These are likely the interim types, between mannan-binding lectin and galactose-binding lectin. We evaluated the expression pattern of EALecl in 12 different tissues using RT-PCR. EALecl was expressed in all tissues, though at different levels. In addition, we inserted EALecl into an expression vector (pET-28a) for transformation into the BL21 engineering bacteria. Based on enzyme digestion and sequencing of the positive clone, we successfully constructed the EALecl recombinant expression vector.展开更多
In order to accurately describe the dynamic characteristics of flight vehicles through aerodynamic modeling, an adaptive wavelet neural network (AWNN) aerodynamic modeling method is proposed, based on subset kernel pr...In order to accurately describe the dynamic characteristics of flight vehicles through aerodynamic modeling, an adaptive wavelet neural network (AWNN) aerodynamic modeling method is proposed, based on subset kernel principal components analysis (SKPCA) feature extraction. Firstly, by fuzzy C-means clustering, some samples are selected from the training sample set to constitute a sample subset. Then, the obtained samples subset is used to execute SKPCA for extracting basic features of the training samples. Finally, using the extracted basic features, the AWNN aerodynamic model is established. The experimental results show that, in 50 times repetitive modeling, the modeling ability of the method proposed is better than that of other six methods. It only needs about half the modeling time of KPCA-AWNN under a close prediction accuracy, and can easily determine the model parameters. This enables it to be effective and feasible to construct the aerodynamic modeling for flight vehicles.展开更多
A three-stage MMIC power amplifier operating from 6to 18GHz is fabricated using 0.25μm A1GaAs/InGaAs/GaAs pseudomorphic high electron mobility transistor(PHEMT).The amplifier isfully monolithic,with all matching,bi...A three-stage MMIC power amplifier operating from 6to 18GHz is fabricated using 0.25μm A1GaAs/InGaAs/GaAs pseudomorphic high electron mobility transistor(PHEMT).The amplifier isfully monolithic,with all matching,biasing,and DC block circuitry included on the chip.Thepower amplifier has an average power gain of 19dB over 6~18GHz.At operation frequenciesfrom 6 to 18GHz,the output power is above 33.3dBm,and the maximum output power of the MMICis 34.7dBm at 10Ghz.The input return loss is less than-10db and the out-put return is lessthan-6dB over operating frequency.This power amplifier has,to our knowledge,the best powergain flatness reported at C-X-Ku-band applications.展开更多
传统CO_2的捕集方法存在设备投资大、环境污染严重、再生能耗高等缺点,利用新型绿色工质离子液体负载于聚偏氟乙烯上制备离子液体支撑膜可以较好地解决该问题。在压力为0.2 MPa、流量为50 m L·min-1、温度在298~318 K范围内变化时...传统CO_2的捕集方法存在设备投资大、环境污染严重、再生能耗高等缺点,利用新型绿色工质离子液体负载于聚偏氟乙烯上制备离子液体支撑膜可以较好地解决该问题。在压力为0.2 MPa、流量为50 m L·min-1、温度在298~318 K范围内变化时,测定了CO_2在1-乙基-3甲基咪唑双三氟磺酰亚胺盐([Emim][Tf_2N])和1-乙基-3-甲基咪唑醋酸盐([Emim][AC])2种离子液体支撑膜中的渗透系数、溶解度和扩散系数,采用Van’t Hoff方程对溶解度数据进行关联、Arrhenius方程对渗透和扩散系数的数据进行关联。实验结果表明,CO_2在2种离子液体支撑膜中的渗透和扩散系数随着温度的升高而增大,溶解度随着温度的升高而降低。[Emim][Tf_2N]离子液体支撑膜对CO_2的捕集性能优于[Emim][AC]支撑膜,CO_2在[Emim][Tf2N]支撑膜中的溶解度最大值为0.447,在[Emim][AC]离子液体支撑膜中的最大溶解度为0.253,由此可确定离子液体支撑膜分离CO_2的最佳工作温度,为其在工业应用中奠定良好的基础。展开更多
基金Supported by the National Natural Science Foundation of China(61139002)~~
文摘Partition-based clustering with weighted feature is developed in the framework of shadowed sets. The objects in the core and boundary regions, generated by shadowed sets-based clustering, have different impact on the prototype of each cluster. By integrating feature weights, a formula for weight calculation is introduced to the clustering algorithm. The selection of weight exponent is crucial for good result and the weights are updated iteratively with each partition of clusters. The convergence of the weighted algorithms is given, and the feasible cluster validity indices of data mining application are utilized. Experimental results on both synthetic and real-life numerical data with different feature weights demonstrate that the weighted algorithm is better than the other unweighted algorithms.
文摘Total mRNA was extracted from a venom gland of snake Daboia russellii siamensis following the manufacturer's protocol of the PolyATtract System 1000 kit purchased from Promega Biotech. cDNAs encoding C-type lectins were amplified by RT-PCR and subcloned into a pMD18-T vector. Fourteen positive clones were selected for nucleotide sequencing and seven cDNAs encoding various snake venom C-type lectin-like protein precursors, designated as DRS-L1, DRS-L2, DRS-L3, DRS-L4 DRS-L5, DRS-L6 and DRS-L7, were obtained. Amino acid sequences of these proteins were deduced and each contains a carbohydrate recognition domain. Of all the deduced protein sequences, only DRS-L1 seemed to represent a closer sequence similarity to α subunits of other known snake venom C-type lectin-like proteins using the BLAST program. Homology comparison combined with analysis of cysteine position indicate that DRS-L1 and DRS-L2 are probably the light chain LC2 and LC1 of factor X activator from Daboia russellii siamensis venom, respectively. DRS-L3 and DRS-L4 might be the β subunits of higher molecular weight C-type lectin-like proteins while DRS-L5 and DRS-L6 might be β subunits of lower molecular weight C-type lectin-like proteins. DRS-L7 might be the β subunit of a platelet membrane glycoprotein Ib-binding protein similar to echicetin.
基金Supported by the National Basic Research Program of China (973 Program) (No. 2006CB101804)the National Natural Science Foundation of China (No. 30972245)Public Industry (Agriculture) Specific Research Program (No. 200803012)
文摘Acting as one of the pattern recognition receptors (PRRs), C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene (named LvLecl) was cloned from the shrimp Litopenaeus vannamei, The ORF of LvLecl is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain (CRD) at the C-terminal. LvLecl was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLecl transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS, Micrococcus lysodeikticus and white spot syndrome virus (WSSV). RNAi-based silencing of LvLecl resulted in increases in mortality when the shrimp were challenged with WSSV, and the median lethal time was reduced compared with controls. Although there was no characteristic "EPN" (Glu-Pro-Ser) or "QPD" (Gin-Pro-Asp) motif, the recombinant LvLecl, expressed in Escherichia coli BL21 (DE3), could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose, D-glucose, D-galactose and N-Acetyl-D-mannose. These results suggest that LvLecl might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei.
文摘A seroprevalence investigation of human brucellosis was carried out in Kuku Dairy Scheme, Sudan. A total of 176 serum samples were collected and screened by Rose Bengal Plate Test (RBPT). The positive sera vqere further examined using Tube Agglutination Test (TAT) and c-Elisa. The seropositivity was 15.9%, 14.8% and 11.4% using RBPT, TAT and c-Elisa respectively. Whereas, the active infection based on seropositivity and clinical signs were 4.6%, 4.6% and 2.3% in case of RBPT, TAT and c-Elisa respectively. Based on c-Elisa result the infected individuals were further subjected to clinical examination and treated with streptomycin and doxocycline for six weeks.
基金Supported by the Natural Science Foundation of Fujian Province of China (No 2060203)New Century Excellent Talents supporting funding of Fujian Province
文摘Lectins play a crucial role in the innate immunity of invertebrates and vertebrates by recognizing and disposing of pathogens. We obtained the complete cDNA of a C-type lectin (EALecl) from Epinephelus akaara using RACE. The complete EALecl cDNA sequence was 827 bp. The 5-UTR and 3-UTR were 28 bp and 151 bp, respectively, in length. The sequence also contained a polyadenylation signal AATAAA and a poly(A) tail. The EALecl cDNA encodes polypeptides with 215 amino acids, including a signal peptide of 31 amino acids. The protein has a cysteine-rich region at the N terminal, a collagenous region characterized by G-X-Y repeats, a neck region, and a typical carbohydrate-recognition domain (CRD), indicating that EALecl is a collectin. The key recognition positions of this CRD are EPD, isolated for the first time in fish. These are likely the interim types, between mannan-binding lectin and galactose-binding lectin. We evaluated the expression pattern of EALecl in 12 different tissues using RT-PCR. EALecl was expressed in all tissues, though at different levels. In addition, we inserted EALecl into an expression vector (pET-28a) for transformation into the BL21 engineering bacteria. Based on enzyme digestion and sequencing of the positive clone, we successfully constructed the EALecl recombinant expression vector.
基金Project(51209167) supported by Youth Project of the National Natural Science Foundation of ChinaProject(2012JM8026) supported by Shaanxi Provincial Natural Science Foundation, China
文摘In order to accurately describe the dynamic characteristics of flight vehicles through aerodynamic modeling, an adaptive wavelet neural network (AWNN) aerodynamic modeling method is proposed, based on subset kernel principal components analysis (SKPCA) feature extraction. Firstly, by fuzzy C-means clustering, some samples are selected from the training sample set to constitute a sample subset. Then, the obtained samples subset is used to execute SKPCA for extracting basic features of the training samples. Finally, using the extracted basic features, the AWNN aerodynamic model is established. The experimental results show that, in 50 times repetitive modeling, the modeling ability of the method proposed is better than that of other six methods. It only needs about half the modeling time of KPCA-AWNN under a close prediction accuracy, and can easily determine the model parameters. This enables it to be effective and feasible to construct the aerodynamic modeling for flight vehicles.
文摘A three-stage MMIC power amplifier operating from 6to 18GHz is fabricated using 0.25μm A1GaAs/InGaAs/GaAs pseudomorphic high electron mobility transistor(PHEMT).The amplifier isfully monolithic,with all matching,biasing,and DC block circuitry included on the chip.Thepower amplifier has an average power gain of 19dB over 6~18GHz.At operation frequenciesfrom 6 to 18GHz,the output power is above 33.3dBm,and the maximum output power of the MMICis 34.7dBm at 10Ghz.The input return loss is less than-10db and the out-put return is lessthan-6dB over operating frequency.This power amplifier has,to our knowledge,the best powergain flatness reported at C-X-Ku-band applications.