Mass microalgal culture plays an irreplaceable role in aquaculture,but microalgal productivity is restricted by traditional autotrophic culture conditions.In the present study,a Tetraselmis chuii strain belonging to t...Mass microalgal culture plays an irreplaceable role in aquaculture,but microalgal productivity is restricted by traditional autotrophic culture conditions.In the present study,a Tetraselmis chuii strain belonging to the phylum Chlorophyta was isolated from south Yellow Sea.The growth rate and biomass productivity of this strain was higher under mixotrophic conditions with different carbon:nitrogen(C:N)ratios than those under autotrophic conditions.When the C:N ratio was 16,the optical density and biomass productivity were 3.7-and 5-fold higher than their corresponding values under autotrophic culture conditions,respectively.Moreover,T.chuii synthesized more polysaccharides and total lipids under mixotrophic conditions.In addition,T.chuii cultured under mixotrophic conditions synthesized more types of fatty acids than autotrophic culture conditions.At a C:N ratio of 16,the percentage of C16:0 and C18:1 reached 30.08%and 24.65%of the total fatty acid(TFA) content,respectively.These findings may provide a basis for largescale mixotrophic culture of T.chuii,as a potential bait-microalga.展开更多
Natural assemblages of marine bacteria were chosen in a batch culture experiments. The impact of varying nitrogen substrate concentrations and the substrate C：N ratios （C：Ns） on the bacterial C：N ratio （C：NB）,...Natural assemblages of marine bacteria were chosen in a batch culture experiments. The impact of varying nitrogen substrate concentrations and the substrate C：N ratios （C：Ns） on the bacterial C：N ratio （C：NB）, the bacterial growth efficiency （BGE） and ammonium regeneration was mainly examined. The C：Ns ratios varied from 5：1 （carbon limitation） to 40：1 （nitrogen limitation） with varying combinations of glucose and NO3. The C：NB ratio had positive relationship with the C：Ns ratio （r=0.93, n=8）, whose value was 3.77 when the C：Ns ratio was 5：1 but increased to 6.47 when the C：Ns ratio was 40：1. These results indicate that the C：NB ratio is a potential diagnostic tool for determining the bacterial growth in natural waters controlled by either, carbon or nitrogen. BGE decreased with the declining nitrate concentration and negatively related to C：N8 （r=-0.51, n=8）. The average value of BGE was 0.20. This value was a little lower than other reports, which could be induced by the nitrogen source used in our experiments. Finally, regeneration time of ammonium delayed with the increasing C：Ns ratio, which indicates that there were different metabolism mechanisms when bacterial growth was limited by carbon source and nitrogen source.展开更多
A hydroponics experiment was conducted to investigate the rice root growth in FACE （free-air carbon dioxide enrichment）. The root biomass, root volume, ratio of root/shoot, number of adventitious roots and root diam...A hydroponics experiment was conducted to investigate the rice root growth in FACE （free-air carbon dioxide enrichment）. The root biomass, root volume, ratio of root/shoot, number of adventitious roots and root diameter significantly increased under FACE conditions, while the CO2 enrichment decreased the N concentration in rice roots without any change in the C content, leading to an increase in root C：N ratio. Moreover, the elevated CO2 resulted in a remarkable decrease of root activity, expressed as per unit root dry weight, which might be responsible for decreased N concentration in roots.展开更多
通过外加氮源或将杉木针叶与林下植物叶混合来改变杉木林凋落物中针叶的养分状况,与杉木林针叶凋落物分解进行比较,分析针叶养分状况及其对杉木林凋落物分解速率和养分释放的影响.结果表明:将杉木针叶与林下植物叶混合和外加氮源均对凋...通过外加氮源或将杉木针叶与林下植物叶混合来改变杉木林凋落物中针叶的养分状况,与杉木林针叶凋落物分解进行比较,分析针叶养分状况及其对杉木林凋落物分解速率和养分释放的影响.结果表明:将杉木针叶与林下植物叶混合和外加氮源均对凋落物分解有不同程度的促进作用.经过153 d的分解后,未经处理的杉木针叶干质量损失率为20.49%,与林下植物叶混合的凋落物干质量损失率为43.67%,其促进作用最大;外加4 gNaNO3的促进作用次之,凋落物干质量损失率为42.07%;外加2 g NaNO3的凋落物干质量损失率为29.13%.对分解过程中各试验方案的凋落物干质量保留率进行方差分析,在开始的62 d内,与林下植被叶混合的杉木针叶凋落物分解速率和其他3种处理之间的差异显著,62 d后未经处理的杉木针叶与加2 g NaNO3的凋落物的分解速率没有显著差异,它们与加4 g NaNO3或林下植物叶的凋落物的分解速率差异显著.凋落物分解速率与凋落物初始C:N比值存在显著的线性关系.外加N源和与林下植物叶混合后,凋落物N的含量增加0.6～1.6倍,C:N比值下降0.4～0.6倍,凋落物底物质量提高,分解速率增大.分解过程中,C质量不断下降,损失24.7%～47.4%,杉木针叶中N出现富集作用,外加N源和与林下植物叶混合的凋落物N释放一定数量后保持稳定的状态.可见,外加适量N源和与林下植物叶混合能提高凋落物底物质量,促进凋落物分解和养分的释放,对维持杉木林的土壤肥力有着重要作用.展开更多
In nutrient-limited alpine meadows,nitrogen(N) mineralization is prior to soil microbial immobilization;therefore,increased mineral N supply would be most likely immobilized by soil microbes due to nutrient shortage i...In nutrient-limited alpine meadows,nitrogen(N) mineralization is prior to soil microbial immobilization;therefore,increased mineral N supply would be most likely immobilized by soil microbes due to nutrient shortage in alpine soils.In addition,low temperature in alpine meadows might be one of the primary factors limiting soil organic matter decomposition and thus N mineralization.A laboratory incubation experiment was performed using an alpine meadow soil from the Tibetan Plateau.Two levels of NH4NO3(N) or glucose(C) were added,with a blank without addition of C or N as the control,before incubation at 5,15,or 25 ℃ for 28 d.CO2 efflux was measured during the 28-d incubation,and the mineral N was measured at the beginning and end of the incubation,in order to test two hypotheses:1) net N mineralization is negatively correlated with CO2 efflux for the control and 2) the external labile N or C supply will shift the negative correlation to positive.The results showed a negative correlation between CO2 efflux and net N immobilization in the control.External inorganic N supply did not change the negative correlation.The external labile C supply shifted the linear correlation from negative to positive under the low C addition level.However,under the high C level,no correlation was found.These suggested that the correlation of CO2 efflux to net N mineralization strongly depend on soil labile C and C:N ratio regardless of temperatures.Further research should focus on the effects of the types and the amount of litter components on interactions of C and N during soil organic matter decomposition.展开更多
Global climate models have indicated high probability of drought occurrences in the coming future decades due to the impacts of climate change caused by a mass release of CO_2. Thus, climate change regarding elevated ...Global climate models have indicated high probability of drought occurrences in the coming future decades due to the impacts of climate change caused by a mass release of CO_2. Thus, climate change regarding elevated ambient CO_2 and drought may consequently affect the growth of crops. In this study, plant physiology, soil carbon, and soil enzyme activities were measured to investigate the impacts of elevated CO_2 and drought stress on a Stagnic Anthrosol planted with soybean(Glycine max). Treatments of two CO_2 levels, three soil moisture levels, and two soil cover types were established. The results indicated that elevated CO_2 and drought stress significantly affected plant physiology. The inhibition of plant physiology by drought stress was mediated via prompted photosynthesis and water use efficiency under elevated CO_2 conditions. Elevated CO_2 resulted in a longer retention time of dissolved organic carbon(DOC) in soil, probably by improving the soil water effectiveness for organic decomposition and mineralization. Drought stress significantly decreased C:N ratio and microbial biomass carbon(MBC), but the interactive effects of drought stress and CO_2 on them were not significant. Elevated CO_2 induced an increase in invertase and catalase activities through stimulated plant root exudation.These results suggested that drought stress had significant negative impacts on plant physiology, soil carbon, and soil enzyme activities,whereas elevated CO_2 and plant physiological feedbacks indirectly ameliorated these impacts.展开更多
基金Supported by the National Basic Research Program of China(973Program)(Nos.2011CB200901,2011CB200904)the National Key Technology Research and Development Program(No.2011BAD14B01)+1 种基金the National High Technology Research and Development Program of China(863 Program)(No.2013AA065801)the Special Foundation for Marine Renewable Energy(No.GHME2011SW03)
文摘Mass microalgal culture plays an irreplaceable role in aquaculture,but microalgal productivity is restricted by traditional autotrophic culture conditions.In the present study,a Tetraselmis chuii strain belonging to the phylum Chlorophyta was isolated from south Yellow Sea.The growth rate and biomass productivity of this strain was higher under mixotrophic conditions with different carbon:nitrogen(C:N)ratios than those under autotrophic conditions.When the C:N ratio was 16,the optical density and biomass productivity were 3.7-and 5-fold higher than their corresponding values under autotrophic culture conditions,respectively.Moreover,T.chuii synthesized more polysaccharides and total lipids under mixotrophic conditions.In addition,T.chuii cultured under mixotrophic conditions synthesized more types of fatty acids than autotrophic culture conditions.At a C:N ratio of 16,the percentage of C16:0 and C18:1 reached 30.08%and 24.65%of the total fatty acid(TFA) content,respectively.These findings may provide a basis for largescale mixotrophic culture of T.chuii,as a potential bait-microalga.
基金The Key Program of International Science and Technology Cooperation from the Ministry of Science and Technology of China under contract No.2004DFA03600the Plan of National Key Basic Research and Development Program Item of China under contract No.2001CB409703the Qingdao Natural Science Foundation of China under contract No. 04-2-JZ-88
文摘Natural assemblages of marine bacteria were chosen in a batch culture experiments. The impact of varying nitrogen substrate concentrations and the substrate C：N ratios （C：Ns） on the bacterial C：N ratio （C：NB）, the bacterial growth efficiency （BGE） and ammonium regeneration was mainly examined. The C：Ns ratios varied from 5：1 （carbon limitation） to 40：1 （nitrogen limitation） with varying combinations of glucose and NO3. The C：NB ratio had positive relationship with the C：Ns ratio （r=0.93, n=8）, whose value was 3.77 when the C：Ns ratio was 5：1 but increased to 6.47 when the C：Ns ratio was 40：1. These results indicate that the C：NB ratio is a potential diagnostic tool for determining the bacterial growth in natural waters controlled by either, carbon or nitrogen. BGE decreased with the declining nitrate concentration and negatively related to C：N8 （r=-0.51, n=8）. The average value of BGE was 0.20. This value was a little lower than other reports, which could be induced by the nitrogen source used in our experiments. Finally, regeneration time of ammonium delayed with the increasing C：Ns ratio, which indicates that there were different metabolism mechanisms when bacterial growth was limited by carbon source and nitrogen source.
基金National Natural Science Foundation of China （nos. 40231003 , 40110817） Knowledge Innovation Program of Chinese Academy of Sciences （no. KZCX2-408） the National Key Project on Basic Sciences （no. 2002CB714003）.
文摘A hydroponics experiment was conducted to investigate the rice root growth in FACE （free-air carbon dioxide enrichment）. The root biomass, root volume, ratio of root/shoot, number of adventitious roots and root diameter significantly increased under FACE conditions, while the CO2 enrichment decreased the N concentration in rice roots without any change in the C content, leading to an increase in root C：N ratio. Moreover, the elevated CO2 resulted in a remarkable decrease of root activity, expressed as per unit root dry weight, which might be responsible for decreased N concentration in roots.
文摘通过外加氮源或将杉木针叶与林下植物叶混合来改变杉木林凋落物中针叶的养分状况,与杉木林针叶凋落物分解进行比较,分析针叶养分状况及其对杉木林凋落物分解速率和养分释放的影响.结果表明:将杉木针叶与林下植物叶混合和外加氮源均对凋落物分解有不同程度的促进作用.经过153 d的分解后,未经处理的杉木针叶干质量损失率为20.49%,与林下植物叶混合的凋落物干质量损失率为43.67%,其促进作用最大;外加4 gNaNO3的促进作用次之,凋落物干质量损失率为42.07%;外加2 g NaNO3的凋落物干质量损失率为29.13%.对分解过程中各试验方案的凋落物干质量保留率进行方差分析,在开始的62 d内,与林下植被叶混合的杉木针叶凋落物分解速率和其他3种处理之间的差异显著,62 d后未经处理的杉木针叶与加2 g NaNO3的凋落物的分解速率没有显著差异,它们与加4 g NaNO3或林下植物叶的凋落物的分解速率差异显著.凋落物分解速率与凋落物初始C:N比值存在显著的线性关系.外加N源和与林下植物叶混合后,凋落物N的含量增加0.6～1.6倍,C:N比值下降0.4～0.6倍,凋落物底物质量提高,分解速率增大.分解过程中,C质量不断下降,损失24.7%～47.4%,杉木针叶中N出现富集作用,外加N源和与林下植物叶混合的凋落物N释放一定数量后保持稳定的状态.可见,外加适量N源和与林下植物叶混合能提高凋落物底物质量,促进凋落物分解和养分的释放,对维持杉木林的土壤肥力有着重要作用.
基金Supported by the National Basic Research Program (973 Program) of China (Nos.2010CB951704 and 2010CB833502)the National Natural Science Foundation for Young Scientists of China (No.30600070)the West Light Joint Scholarship of the Chinese Academy of Sciences
文摘In nutrient-limited alpine meadows,nitrogen(N) mineralization is prior to soil microbial immobilization;therefore,increased mineral N supply would be most likely immobilized by soil microbes due to nutrient shortage in alpine soils.In addition,low temperature in alpine meadows might be one of the primary factors limiting soil organic matter decomposition and thus N mineralization.A laboratory incubation experiment was performed using an alpine meadow soil from the Tibetan Plateau.Two levels of NH4NO3(N) or glucose(C) were added,with a blank without addition of C or N as the control,before incubation at 5,15,or 25 ℃ for 28 d.CO2 efflux was measured during the 28-d incubation,and the mineral N was measured at the beginning and end of the incubation,in order to test two hypotheses:1) net N mineralization is negatively correlated with CO2 efflux for the control and 2) the external labile N or C supply will shift the negative correlation to positive.The results showed a negative correlation between CO2 efflux and net N immobilization in the control.External inorganic N supply did not change the negative correlation.The external labile C supply shifted the linear correlation from negative to positive under the low C addition level.However,under the high C level,no correlation was found.These suggested that the correlation of CO2 efflux to net N mineralization strongly depend on soil labile C and C:N ratio regardless of temperatures.Further research should focus on the effects of the types and the amount of litter components on interactions of C and N during soil organic matter decomposition.
基金supported by the National Natural Science Foundation of China (No.51309053)the Fundamental Research Funds for the Central Universities-Donghua University (DHU) Distinguished Young Professor Program, China (No.B201310)
文摘Global climate models have indicated high probability of drought occurrences in the coming future decades due to the impacts of climate change caused by a mass release of CO_2. Thus, climate change regarding elevated ambient CO_2 and drought may consequently affect the growth of crops. In this study, plant physiology, soil carbon, and soil enzyme activities were measured to investigate the impacts of elevated CO_2 and drought stress on a Stagnic Anthrosol planted with soybean(Glycine max). Treatments of two CO_2 levels, three soil moisture levels, and two soil cover types were established. The results indicated that elevated CO_2 and drought stress significantly affected plant physiology. The inhibition of plant physiology by drought stress was mediated via prompted photosynthesis and water use efficiency under elevated CO_2 conditions. Elevated CO_2 resulted in a longer retention time of dissolved organic carbon(DOC) in soil, probably by improving the soil water effectiveness for organic decomposition and mineralization. Drought stress significantly decreased C:N ratio and microbial biomass carbon(MBC), but the interactive effects of drought stress and CO_2 on them were not significant. Elevated CO_2 induced an increase in invertase and catalase activities through stimulated plant root exudation.These results suggested that drought stress had significant negative impacts on plant physiology, soil carbon, and soil enzyme activities,whereas elevated CO_2 and plant physiological feedbacks indirectly ameliorated these impacts.
