目的:通过研究银屑病患者皮损处真皮间充质干细胞(DMSCs)中磷脂酶C-β4(PLCB4)和视黄醇脱氢酶(RDH)10mRNA的表达水平,进一步探讨银屑病发病的细胞及分子机制。方法:对24例寻常性银屑病(PV)患者和22例正常人皮肤DMSCs进行分离培养,流式...目的:通过研究银屑病患者皮损处真皮间充质干细胞(DMSCs)中磷脂酶C-β4(PLCB4)和视黄醇脱氢酶(RDH)10mRNA的表达水平,进一步探讨银屑病发病的细胞及分子机制。方法:对24例寻常性银屑病(PV)患者和22例正常人皮肤DMSCs进行分离培养,流式细胞术进行细胞表型鉴定,实时荧光定量聚合酶链式反应(PCR)测定PLCB4和RDH10 mRNA表达水平。结果:PV组与正常对照组DMSCs形态无差异。PV组患者DMSCs中PLCB4 m RNA的表达水平是正常对照组的3.35倍,RDH10 mRNA的表达水平是正常对照组的1.17倍。结论:银屑病患者DMSCs中PLCB4和RDH10 m RNA表达均增高,可能影响相关信号通路,进而对银屑病发病产生影响。展开更多
目的:探讨C-反应蛋白(C-reactive protein,CRP)及γ-干扰素诱生蛋白10(Interferonγ-inducible protein 10,IP-10)在新生儿感染性疾病中的变化及临床意义。方法:应用比浊法及ELISA法对40例感染性疾病新生儿及40例足月健康新生儿行血清CR...目的:探讨C-反应蛋白(C-reactive protein,CRP)及γ-干扰素诱生蛋白10(Interferonγ-inducible protein 10,IP-10)在新生儿感染性疾病中的变化及临床意义。方法:应用比浊法及ELISA法对40例感染性疾病新生儿及40例足月健康新生儿行血清CRP和IP-10测定,并比较临床意义。结果:治疗前感染组血清CRP、IP-10分别为(57.25±47.81)mg/L和(582.37±147.35)ng/L,而对照组分别为(1.52±0.63)mg/L和(92.71±58.29)ng/L,感染组明显高于对照组(P<0.05);而治疗后感染组血清CRP和IP-10降低,恢复到正常范围,与对照组比较,差异无统计学意义(P>0.05)。结论:血清CRP及IP-10可作为判定新生儿早期感染的指标之一,且动态观察CRP及IP-10可作为感染程度和疗效的判断标准,从而可提高新生儿早期感染性疾病的诊疗率。展开更多
In the lower parts of oil reservoirs Chang 9 and Chang 10 of the Yanchang Formation are oil-bearing layers newly found in oil exploration in the Ordos Basin.Based on GC,GC-MS analyses of saturated hydrocarbons from cr...In the lower parts of oil reservoirs Chang 9 and Chang 10 of the Yanchang Formation are oil-bearing layers newly found in oil exploration in the Ordos Basin.Based on GC,GC-MS analyses of saturated hydrocarbons from crude oils and source rocks,reservoir fluid inclusions and BasinMod,the origin of crude oils,accumulation period and accumulation models are discussed in combination with other petroleum geology data in this paper.The result shows that(1) there are two different types of crude oils in oil reservoir Chang 9 in the Longdong and Jiyuan regions:crude oils of typeⅠ(Well D86,Well A44,Well A75,Well B227,Well X62 and Well Z150) are mainly de-rived from the Chang 7 source rocks(including mudstones and shales) and distributed in the Jiyuan and Longdong regions;those of typeⅡ(Well Z14 and Well Y427),are distributed in the Longdong region,which are derived from the Chang 9 source rocks.Crude oils from oil reservoir Chang 10 in the Shanbei region are mainly derived from the Chang-9 source rocks;(2) there are two phases of hydrocarbon filling in oil reservoir Chang 9 in the Jiyuan and Longdong regions and oil reservoir Chang 10 in the Shanbei region:The first phase started at the early stage of J2z.The process of hydrocarbon filling was discontinuous in the Late Jurassic,because of the tectonic-thermal event in the Ordos Basin.The second phase was the main accumulation period,and hydrocarbons began to accumulate from the late stage of J2a to the middle-late of K1,mainly at the middle-late stage of K1;(3) there exist two types of accu-mulation models in oil reservoirs Chang 9 and Chang 10 of the Yanchang Formation:source rocks of the reservoirs in oil reservoir Chang 9 in the Jiyuan region and oil reservoir Chang 10 in the Shanbei region,the mixed type of reservoirs on the lateral side of source rocks and source rocks of the reservoirs in oil reservoir Chang 9 in the Long-dong region.展开更多
文摘目的:通过研究银屑病患者皮损处真皮间充质干细胞(DMSCs)中磷脂酶C-β4(PLCB4)和视黄醇脱氢酶(RDH)10mRNA的表达水平,进一步探讨银屑病发病的细胞及分子机制。方法:对24例寻常性银屑病(PV)患者和22例正常人皮肤DMSCs进行分离培养,流式细胞术进行细胞表型鉴定,实时荧光定量聚合酶链式反应(PCR)测定PLCB4和RDH10 mRNA表达水平。结果:PV组与正常对照组DMSCs形态无差异。PV组患者DMSCs中PLCB4 m RNA的表达水平是正常对照组的3.35倍,RDH10 mRNA的表达水平是正常对照组的1.17倍。结论:银屑病患者DMSCs中PLCB4和RDH10 m RNA表达均增高,可能影响相关信号通路,进而对银屑病发病产生影响。
文摘目的:探讨C-反应蛋白(C-reactive protein,CRP)及γ-干扰素诱生蛋白10(Interferonγ-inducible protein 10,IP-10)在新生儿感染性疾病中的变化及临床意义。方法:应用比浊法及ELISA法对40例感染性疾病新生儿及40例足月健康新生儿行血清CRP和IP-10测定,并比较临床意义。结果:治疗前感染组血清CRP、IP-10分别为(57.25±47.81)mg/L和(582.37±147.35)ng/L,而对照组分别为(1.52±0.63)mg/L和(92.71±58.29)ng/L,感染组明显高于对照组(P<0.05);而治疗后感染组血清CRP和IP-10降低,恢复到正常范围,与对照组比较,差异无统计学意义(P>0.05)。结论:血清CRP及IP-10可作为判定新生儿早期感染的指标之一,且动态观察CRP及IP-10可作为感染程度和疗效的判断标准,从而可提高新生儿早期感染性疾病的诊疗率。
文摘In the lower parts of oil reservoirs Chang 9 and Chang 10 of the Yanchang Formation are oil-bearing layers newly found in oil exploration in the Ordos Basin.Based on GC,GC-MS analyses of saturated hydrocarbons from crude oils and source rocks,reservoir fluid inclusions and BasinMod,the origin of crude oils,accumulation period and accumulation models are discussed in combination with other petroleum geology data in this paper.The result shows that(1) there are two different types of crude oils in oil reservoir Chang 9 in the Longdong and Jiyuan regions:crude oils of typeⅠ(Well D86,Well A44,Well A75,Well B227,Well X62 and Well Z150) are mainly de-rived from the Chang 7 source rocks(including mudstones and shales) and distributed in the Jiyuan and Longdong regions;those of typeⅡ(Well Z14 and Well Y427),are distributed in the Longdong region,which are derived from the Chang 9 source rocks.Crude oils from oil reservoir Chang 10 in the Shanbei region are mainly derived from the Chang-9 source rocks;(2) there are two phases of hydrocarbon filling in oil reservoir Chang 9 in the Jiyuan and Longdong regions and oil reservoir Chang 10 in the Shanbei region:The first phase started at the early stage of J2z.The process of hydrocarbon filling was discontinuous in the Late Jurassic,because of the tectonic-thermal event in the Ordos Basin.The second phase was the main accumulation period,and hydrocarbons began to accumulate from the late stage of J2a to the middle-late of K1,mainly at the middle-late stage of K1;(3) there exist two types of accu-mulation models in oil reservoirs Chang 9 and Chang 10 of the Yanchang Formation:source rocks of the reservoirs in oil reservoir Chang 9 in the Jiyuan region and oil reservoir Chang 10 in the Shanbei region,the mixed type of reservoirs on the lateral side of source rocks and source rocks of the reservoirs in oil reservoir Chang 9 in the Long-dong region.
