Androgens play a central role in prostate cancer pathogenesis, and hence most of the patients respond to androgen deprivation therapies. However, patients tend to relapse with aggressive prostate cancer, which has bee...Androgens play a central role in prostate cancer pathogenesis, and hence most of the patients respond to androgen deprivation therapies. However, patients tend to relapse with aggressive prostate cancer, which has been termed as hormone refractory. To identify the proteins that mediate progression to the hormone-refractory state, we used protein-chip technology for mass profiling of patients＇ sera. This study included 16 patients with metastatic hormone-refractory prostate cancer who were initially treated with androgen deprivation therapy. Serum samples were collected from each patient at five time points： point A, pre-treatment; point B, at the nadir of the prostate- specific antigen （PSA） level; point C, PSA failure; point D, the early hormone-refractory phase; and point E, the late hormone-refractory phase. Using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, we performed protein mass profiling of the patients＇ sera and identified a 6 640-Da peak that increased with disease progression. Target proteins were partially purified, and by amino acid sequencing the peak was identified as a fragment of apolipoprotein C-I （ApoC-I）. Serum ApoC-I protein levels increased with disease progression. On immunohistochemical analysis, the ApoC-i protein was found localized to the cytoplasm of the hormone-refractory cancer cells. In this study, we showed an increase in serum ApoC-I protein levels in prostate cancer patients during their progression to the hormone-refractory state, which suggests that ApoC-I protein is related to progression of prostate cancer. However, as the exact role of ApoC-I in prostate cancer pathogenesis is unclear, further research is required.展开更多
A rabbit anti serum to tree shrew apolipoprotein C I (apo C I) was used to screen an expression cDNA library constructed by us from tree shrew (TS)liver tissue. Two apo C I cDNA clones were obtained. The longer one co...A rabbit anti serum to tree shrew apolipoprotein C I (apo C I) was used to screen an expression cDNA library constructed by us from tree shrew (TS)liver tissue. Two apo C I cDNA clones were obtained. The longer one consistsof 380 nucleotides, including 21 bp and 95 bp at the 5’ and 3’ end of the non translated regions respectively, and a 264 bp fragment in an open reading frame encoding 88 amino acids prepropeptide which contains 26 amino acids of signal peptide and a mature protein (62 amino acids). Comparing the amino acid sequence deduced from this cDNA with those of the published mammalian apo C Is reveals that it shared some structural similarity with rat, mouse and dog apo C I, but it had 5 more amino acids than that of human and baboon. The expression of apo C I mRNA in 8 different tissues were also assayed with Northern blot. The results demonstrated that liver had the highest expression, intestine had much less expression and no expression in other tissues, which is much different from human and other species. This study has laid down a good foundation for further studying on the function and the stucture of tree shrew apo C I gene.展开更多
AIM To investigate the effect of(-)-epigallocatechin-3-gallate(EGCG) on polyinosinic-polycytidylic acid(poly I:C)-triggered intracellular innate immunity against hepatitis C virus(HCV) in hepatocytes. METHODS A cell c...AIM To investigate the effect of(-)-epigallocatechin-3-gallate(EGCG) on polyinosinic-polycytidylic acid(poly I:C)-triggered intracellular innate immunity against hepatitis C virus(HCV) in hepatocytes. METHODS A cell culture model of HCV infection was generated by infecting a hepatoma cell line, Huh7, with HCV JFH-1 strain(JFH-1-Huh7). Poly I:C with a high molecular weight and EGCG were used to stimulate the JFH-1-Huh7 cells. Real-time reverse transcription-polymerase chain reaction was used to detect the expression levels of intracellular m RNAs and of intracellular and extracellular HCV RNA. Enzyme-linked immunosorbent assay was used to evaluate the interferon(IFN)-λ1 protein level in the cell culture supernatant. Immunostaining was used to examine HCV core protein expression in Huh7 cells.RESULTS Our recent study showed that HCV replication could impair poly I:C-triggered intracellular innate immune responses in hepatocytes. In the current study, we showed that EGCG treatment significantly increased the poly I:C-induced expression of Toll-like receptor 3(TLR3), retinoic acid-inducible gene I, and IFN-λ1 in JFH-1-Huh7 cells. In addition, supplementation with EGCG increased the poly I:C-mediated antiviral activity in JFH-1-Huh7 cells at the intracellular and extracellular HCV RNA and protein levels. Further investigation of the mechanisms showed that EGCG treatment significantly enhanced the poly I:C-induced expression of IFN-regulatory factor 9 and several antiviral IFNstimulated genes, including ISG15, ISG56, myxovirus resistance A, and 2'-5'-oligoadenylate synthetase 1, which encode the key antiviral elements in the IFN signaling pathway. CONCLUSION Our observations provide experimental evidence that EGCG has the ability to enhance poly I:C-induced intracellular antiviral innate immunity against HCV replication in hepatocytes.展开更多
双碳政策推动下,乡村农业综合能源系统(integrated energy system,IES)的多能耦合关系更加复杂。为实现农业园区可靠运行,提出面向生态农业IES的多能互补与低碳运行优化调度策略。首先,基于农业园区的能量流动关系,建立沼气生产环节、...双碳政策推动下,乡村农业综合能源系统(integrated energy system,IES)的多能耦合关系更加复杂。为实现农业园区可靠运行,提出面向生态农业IES的多能互补与低碳运行优化调度策略。首先,基于农业园区的能量流动关系,建立沼气生产环节、多能耦合供应环节以及柔性负荷需求响应环节的数学模型。其次,考虑光伏、负荷和沼气的不确定性,建立生态农业IES两阶段鲁棒优化模型。模型引入碳排放成本和启停成本,可降低农业生产碳排放,防止机组频繁启停。然后,采用列与约束生成算法(column-and-constraint generation,C&CG),结合强对偶定理与线性化理论实现模型求解。最后,基于江西省某生态牧场IES进行算例仿真,验证所提策略的有效性。仿真结果表明,所提策略可实现生态农业IES的协调运行,提高系统经济性、低碳性和能效性。展开更多
This work was a retrospective prospective study carried out in the general surgery department of the Cs ref of commune I from January 2009 to December 2012. Our study aimed to determine the hospital frequency of hemor...This work was a retrospective prospective study carried out in the general surgery department of the Cs ref of commune I from January 2009 to December 2012. Our study aimed to determine the hospital frequency of hemorrhoidal disease, to identify the contributing factors, describe the clinical and therapeutic aspects and analyze the surgical consequences. We collected 73 patients with an average age of 37.8 years (range: 19 years and 77 years) with a sex ratio of 2.5 in favor of men. We operated on 49 patients or 67.1% of cases. 71.2% of our patients were married and lived in Bamako. Schoolchildren and middle managers made up 39.8% of cases. 82.2% of patients were seen in ordinary consultation and 69.9% complained of progressive anal pain in 66.7% of cases. This pain was triggered by defecation in 88.2% of our patients. The contributing factors were dominated by constipation (53.4%), working in a seated position (41.1%) and a sedentary lifestyle (16.4%) of cases. Stage IV external hemorrhoids and hemorrhoidal thrombosis represented 53.8% of surgical indications. The most used surgical technique was that of Milligan-Morgan, i.e. 51.9% of cases and 45.2% of patients treated medically received a combination of transit regulator, venotonics and analgesics. The surgical aftermath was marked by hemorrhage (3 cases), delayed healing (5 cases), urinary retention (1 case) and scarring anal stenosis (1 case). The morbidity rate was 5.48% of cases and a zero mortality rate. The average length of hospitalization was 2 days with extremes of 1 and 5 days.展开更多
Inguinal hernia in children is a congenital pathology in children linked to the persistence of the peritoneo-vaginal canal in children and the NüCK canal in girls;persisting into adulthood. This work aimed to stu...Inguinal hernia in children is a congenital pathology in children linked to the persistence of the peritoneo-vaginal canal in children and the NüCK canal in girls;persisting into adulthood. This work aimed to study inguinal hernia in children in the general surgery department of the Reference Health Center of Commune I of the Bamako District;to determine the frequency of inguinal hernia;describe the epidemiological, clinical and therapeutic aspects of inguinal hernias and in order to evaluate the cost of treatment. This was a prospective study from April 2017 to March 2018 during which 60 children with an inguinal hernia were collected. Inguinal hernias in children represented 9.23% of patients operated on in the department. The average age was 59.23 months with extremes of one month and 180 months and a standard deviation of 49.23 years. The male sex was dominant with a sex ratio equal to 9:1. The notion of prematurity was found in 11.7% and was associated with a testicle not in place in 1.7% of cases. 41.7% of our patients were referred by a doctor and 66.7% of patients presented with painless, intermittent inguino-scrotal swelling (83.3% of cases). The hernia was discovered before one week of life in 50% of cases. Inguinal swelling was absent in 11.7% but observed intraoperatively. The inguinal hernia was unilateral in 91.7% of cases and 70% of hernias were discovered by the parents during pushing efforts. The inguinal hernia was located on the right in 61.7% of cases. The hernia swelling was soft, painless, impulsive and reducible in 78.3% of cases. The swelling was inguino-scrotal in 58.3% of cases. The hernia was simple in 95% of patients and hernial strangulation was observed in 3 cases or 5% of cases. 98.3% of patients were ASA I. The treatment was carried out openly in all our patients including closure of the vaginal peritoneal canal in 95% of cases under general anesthesia in 98.3% of cases. The morbidity rate was 8.4% (surgical site infection: 6.7% of cases and hematoma: 1.7%). The immediate consequences were simple in 96.6% of cases. No cases of recurrence occurred during the 6 months after the intervention. The average cost of care was estimated at 69,743 FCFA.展开更多
条形码数据库是开展基于DNA的生物监测关键先决条件。为在珠江流域有效开展基于DNA的生物监测,迫切需要了解物种DNA条形码的覆盖或空缺状况。整理了珠江流域常见鱼类和大型底栖动物的物种清单,从National Center and Biotechnology Info...条形码数据库是开展基于DNA的生物监测关键先决条件。为在珠江流域有效开展基于DNA的生物监测,迫切需要了解物种DNA条形码的覆盖或空缺状况。整理了珠江流域常见鱼类和大型底栖动物的物种清单,从National Center and Biotechnology Information (NCBI)数据库中检索了物种清单的DNA条形码序列,分析了常见鱼类(包括线粒体组和12s rRNA基因)和大型底栖动物(包括线粒体组、COI和18s rRNA基因)的DNA条形码覆盖范围和空缺程度。数据分析表明:(1)珠江流域共记录了常见鱼类221种,隶属于2纲18目51科和137属;常见大型底栖动物105种/属,隶属于6纲14目53科。(2)共检索到常见鱼类线粒体组序列913条和12s rRNA基因序列962条,分别占总物种的81.45%和57.92%;有12.67%的物种没有线粒体组和12s rRNA基因序列,若将条形码阈值设置为至少包含5个参考序列,则空缺度上升至52.94%;(3)共检索到常见大型底栖动物线粒体组65条序列、COI基因26,988条序列和18s rRNA基因175条序列,分别占总种/属数的29.52%、68.57%和37.14%;有25.71%的种/属在线粒体组、COI和18s rRNA基因区域皆无序列收录,若将条形码阈值设置为至少包含5个参考序列,则空缺度上升至41.90%。总之,本研究将为珠江流域开展基于DNA的鱼类和大型底栖动物监测提供基础数据支撑,为完善珠江本土DNA条形码数据库提供参考建议。展开更多
文摘Androgens play a central role in prostate cancer pathogenesis, and hence most of the patients respond to androgen deprivation therapies. However, patients tend to relapse with aggressive prostate cancer, which has been termed as hormone refractory. To identify the proteins that mediate progression to the hormone-refractory state, we used protein-chip technology for mass profiling of patients＇ sera. This study included 16 patients with metastatic hormone-refractory prostate cancer who were initially treated with androgen deprivation therapy. Serum samples were collected from each patient at five time points： point A, pre-treatment; point B, at the nadir of the prostate- specific antigen （PSA） level; point C, PSA failure; point D, the early hormone-refractory phase; and point E, the late hormone-refractory phase. Using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, we performed protein mass profiling of the patients＇ sera and identified a 6 640-Da peak that increased with disease progression. Target proteins were partially purified, and by amino acid sequencing the peak was identified as a fragment of apolipoprotein C-I （ApoC-I）. Serum ApoC-I protein levels increased with disease progression. On immunohistochemical analysis, the ApoC-i protein was found localized to the cytoplasm of the hormone-refractory cancer cells. In this study, we showed an increase in serum ApoC-I protein levels in prostate cancer patients during their progression to the hormone-refractory state, which suggests that ApoC-I protein is related to progression of prostate cancer. However, as the exact role of ApoC-I in prostate cancer pathogenesis is unclear, further research is required.
文摘A rabbit anti serum to tree shrew apolipoprotein C I (apo C I) was used to screen an expression cDNA library constructed by us from tree shrew (TS)liver tissue. Two apo C I cDNA clones were obtained. The longer one consistsof 380 nucleotides, including 21 bp and 95 bp at the 5’ and 3’ end of the non translated regions respectively, and a 264 bp fragment in an open reading frame encoding 88 amino acids prepropeptide which contains 26 amino acids of signal peptide and a mature protein (62 amino acids). Comparing the amino acid sequence deduced from this cDNA with those of the published mammalian apo C Is reveals that it shared some structural similarity with rat, mouse and dog apo C I, but it had 5 more amino acids than that of human and baboon. The expression of apo C I mRNA in 8 different tissues were also assayed with Northern blot. The results demonstrated that liver had the highest expression, intestine had much less expression and no expression in other tissues, which is much different from human and other species. This study has laid down a good foundation for further studying on the function and the stucture of tree shrew apo C I gene.
基金Supported by the National Natural Science Foundation of China,No.81500449the Natural Science Foundation of Shanghai,No.14ZR1434200+2 种基金Shanghai Municipal Commission of Health and Family Planning,No.20144Y0175the Scientific Research Foundation for the Returned Overseas Chinese Scholarsthe State Education Ministry of China,No.20150909-6
文摘AIM To investigate the effect of(-)-epigallocatechin-3-gallate(EGCG) on polyinosinic-polycytidylic acid(poly I:C)-triggered intracellular innate immunity against hepatitis C virus(HCV) in hepatocytes. METHODS A cell culture model of HCV infection was generated by infecting a hepatoma cell line, Huh7, with HCV JFH-1 strain(JFH-1-Huh7). Poly I:C with a high molecular weight and EGCG were used to stimulate the JFH-1-Huh7 cells. Real-time reverse transcription-polymerase chain reaction was used to detect the expression levels of intracellular m RNAs and of intracellular and extracellular HCV RNA. Enzyme-linked immunosorbent assay was used to evaluate the interferon(IFN)-λ1 protein level in the cell culture supernatant. Immunostaining was used to examine HCV core protein expression in Huh7 cells.RESULTS Our recent study showed that HCV replication could impair poly I:C-triggered intracellular innate immune responses in hepatocytes. In the current study, we showed that EGCG treatment significantly increased the poly I:C-induced expression of Toll-like receptor 3(TLR3), retinoic acid-inducible gene I, and IFN-λ1 in JFH-1-Huh7 cells. In addition, supplementation with EGCG increased the poly I:C-mediated antiviral activity in JFH-1-Huh7 cells at the intracellular and extracellular HCV RNA and protein levels. Further investigation of the mechanisms showed that EGCG treatment significantly enhanced the poly I:C-induced expression of IFN-regulatory factor 9 and several antiviral IFNstimulated genes, including ISG15, ISG56, myxovirus resistance A, and 2'-5'-oligoadenylate synthetase 1, which encode the key antiviral elements in the IFN signaling pathway. CONCLUSION Our observations provide experimental evidence that EGCG has the ability to enhance poly I:C-induced intracellular antiviral innate immunity against HCV replication in hepatocytes.
文摘双碳政策推动下,乡村农业综合能源系统(integrated energy system,IES)的多能耦合关系更加复杂。为实现农业园区可靠运行,提出面向生态农业IES的多能互补与低碳运行优化调度策略。首先,基于农业园区的能量流动关系,建立沼气生产环节、多能耦合供应环节以及柔性负荷需求响应环节的数学模型。其次,考虑光伏、负荷和沼气的不确定性,建立生态农业IES两阶段鲁棒优化模型。模型引入碳排放成本和启停成本,可降低农业生产碳排放,防止机组频繁启停。然后,采用列与约束生成算法(column-and-constraint generation,C&CG),结合强对偶定理与线性化理论实现模型求解。最后,基于江西省某生态牧场IES进行算例仿真,验证所提策略的有效性。仿真结果表明,所提策略可实现生态农业IES的协调运行,提高系统经济性、低碳性和能效性。
文摘This work was a retrospective prospective study carried out in the general surgery department of the Cs ref of commune I from January 2009 to December 2012. Our study aimed to determine the hospital frequency of hemorrhoidal disease, to identify the contributing factors, describe the clinical and therapeutic aspects and analyze the surgical consequences. We collected 73 patients with an average age of 37.8 years (range: 19 years and 77 years) with a sex ratio of 2.5 in favor of men. We operated on 49 patients or 67.1% of cases. 71.2% of our patients were married and lived in Bamako. Schoolchildren and middle managers made up 39.8% of cases. 82.2% of patients were seen in ordinary consultation and 69.9% complained of progressive anal pain in 66.7% of cases. This pain was triggered by defecation in 88.2% of our patients. The contributing factors were dominated by constipation (53.4%), working in a seated position (41.1%) and a sedentary lifestyle (16.4%) of cases. Stage IV external hemorrhoids and hemorrhoidal thrombosis represented 53.8% of surgical indications. The most used surgical technique was that of Milligan-Morgan, i.e. 51.9% of cases and 45.2% of patients treated medically received a combination of transit regulator, venotonics and analgesics. The surgical aftermath was marked by hemorrhage (3 cases), delayed healing (5 cases), urinary retention (1 case) and scarring anal stenosis (1 case). The morbidity rate was 5.48% of cases and a zero mortality rate. The average length of hospitalization was 2 days with extremes of 1 and 5 days.
文摘Inguinal hernia in children is a congenital pathology in children linked to the persistence of the peritoneo-vaginal canal in children and the NüCK canal in girls;persisting into adulthood. This work aimed to study inguinal hernia in children in the general surgery department of the Reference Health Center of Commune I of the Bamako District;to determine the frequency of inguinal hernia;describe the epidemiological, clinical and therapeutic aspects of inguinal hernias and in order to evaluate the cost of treatment. This was a prospective study from April 2017 to March 2018 during which 60 children with an inguinal hernia were collected. Inguinal hernias in children represented 9.23% of patients operated on in the department. The average age was 59.23 months with extremes of one month and 180 months and a standard deviation of 49.23 years. The male sex was dominant with a sex ratio equal to 9:1. The notion of prematurity was found in 11.7% and was associated with a testicle not in place in 1.7% of cases. 41.7% of our patients were referred by a doctor and 66.7% of patients presented with painless, intermittent inguino-scrotal swelling (83.3% of cases). The hernia was discovered before one week of life in 50% of cases. Inguinal swelling was absent in 11.7% but observed intraoperatively. The inguinal hernia was unilateral in 91.7% of cases and 70% of hernias were discovered by the parents during pushing efforts. The inguinal hernia was located on the right in 61.7% of cases. The hernia swelling was soft, painless, impulsive and reducible in 78.3% of cases. The swelling was inguino-scrotal in 58.3% of cases. The hernia was simple in 95% of patients and hernial strangulation was observed in 3 cases or 5% of cases. 98.3% of patients were ASA I. The treatment was carried out openly in all our patients including closure of the vaginal peritoneal canal in 95% of cases under general anesthesia in 98.3% of cases. The morbidity rate was 8.4% (surgical site infection: 6.7% of cases and hematoma: 1.7%). The immediate consequences were simple in 96.6% of cases. No cases of recurrence occurred during the 6 months after the intervention. The average cost of care was estimated at 69,743 FCFA.
文摘条形码数据库是开展基于DNA的生物监测关键先决条件。为在珠江流域有效开展基于DNA的生物监测,迫切需要了解物种DNA条形码的覆盖或空缺状况。整理了珠江流域常见鱼类和大型底栖动物的物种清单,从National Center and Biotechnology Information (NCBI)数据库中检索了物种清单的DNA条形码序列,分析了常见鱼类(包括线粒体组和12s rRNA基因)和大型底栖动物(包括线粒体组、COI和18s rRNA基因)的DNA条形码覆盖范围和空缺程度。数据分析表明:(1)珠江流域共记录了常见鱼类221种,隶属于2纲18目51科和137属;常见大型底栖动物105种/属,隶属于6纲14目53科。(2)共检索到常见鱼类线粒体组序列913条和12s rRNA基因序列962条,分别占总物种的81.45%和57.92%;有12.67%的物种没有线粒体组和12s rRNA基因序列,若将条形码阈值设置为至少包含5个参考序列,则空缺度上升至52.94%;(3)共检索到常见大型底栖动物线粒体组65条序列、COI基因26,988条序列和18s rRNA基因175条序列,分别占总种/属数的29.52%、68.57%和37.14%;有25.71%的种/属在线粒体组、COI和18s rRNA基因区域皆无序列收录,若将条形码阈值设置为至少包含5个参考序列,则空缺度上升至41.90%。总之,本研究将为珠江流域开展基于DNA的鱼类和大型底栖动物监测提供基础数据支撑,为完善珠江本土DNA条形码数据库提供参考建议。
