The objective of this study was to evaluate the effect of a novel feed additive on chicken intestinal colonization and carcass contamination by Campylobacterjejuni. The feed additive was composed of microencapsulated ...The objective of this study was to evaluate the effect of a novel feed additive on chicken intestinal colonization and carcass contamination by Campylobacterjejuni. The feed additive was composed of microencapsulated organic acids and essential oils （OA/EO）. The feed additive tested was provided by Jefo Nutrition Inc., St-Hyacinthe, Quebec, Canada. Day-old birds were separated into two rooms and subdivided into two groups. Chicken were fed with OA/EO or not fed with OA/EO until they reached 35 d of age. At 14 d of age, chickens received an oral suspension of two well characterized C. jejuni strains, depending on the room they were housed in. The levels of C. jejuni were periodically monitored in the caecum and on the carcasses. C. jejuni colonization was further characterized by the use of high-resolution melt analysis of the C. jejuniflaA gene （HRM-flaA）. The effect of the feed additive was strain-dependent. In room two, the feed additive had no effect on the caecal counts. In room one, at 35 d of age, caecal C. jejuni counts were higher with OA/EO, as opposed to carcasses counts which were lower in the treated group. The HRM-flaA analysis showed that an amplification profile was predominant in birds fed with OA/EO at 35 d of age in room one, suggesting the selection of a C. jejuni strain. In conclusion, the OA/EO seemed to be effective to reduce C. jejuni levels but this effect appeared strain dependent.展开更多
Campylobacter jejuni infection has been implicated in the pathogenesis of Guillain-Barré syndrome (GBS) due to production of humoral immune response against neural antigens. A case-control study was performed in ...Campylobacter jejuni infection has been implicated in the pathogenesis of Guillain-Barré syndrome (GBS) due to production of humoral immune response against neural antigens. A case-control study was performed in a tertiary care teaching hospital for the estimation of anti-ganglioside antibodies in GBS patients and their controls. Blood samples were collected from 59 GBS cases, 58 neurological controls (NC) and 60 non-neurological control (NNC) patients for automatic estimation of IgG and IgM antibodies to seven gangliosides using EUROLineScan software. Antibodies of IgG class for GM1 were highly significant in GBS (p = 0.000) and NC (p = 0.031) compared to NNC. However GBS group was not significant (p = 0.413) compared to NC. For GM2 ganglioside, GBS and NC groups were significant (p = 0.000) compared to NNC, but GBS group was not significant (p = 0.999) compared to NC. For GM3 ganglioside, GBS and NC groups were significant (p = 0.000) compared to NNC;but GBS group was insignificant (p = 0.858) compared to NC with similar trend for all other ganglioside antibodies. When IgM class of antibodies was evaluated for GM1, GBS group was not significant (p = 0.604) whereas NC group was significant (p = 0.000) compared to NNC. GBS group was not significant compared to NC (p = 0.011). The trend was the same for GM2 antibodies. For GM3, GBS group was significant (p = 0.010) and NC was near significant (p = 0.055) compared to NNC. However GBS group was not significant (p = 0.808) compared to NC. No groups were significant (p > 0.05) in relation to the remaining gangliosides except for GQ1b where GBS group (p = 0.001) and NC group were significant (p = 0.000) compared to NNC. GBS group was also significant (p = 0.001) compared to NC and NNC. Anti-gangliosides antibodies were present in highly significant levels in the GBS group, though they were also present in the non-paralytic neurological control patients compared to the non-neurological control group.展开更多
文摘The objective of this study was to evaluate the effect of a novel feed additive on chicken intestinal colonization and carcass contamination by Campylobacterjejuni. The feed additive was composed of microencapsulated organic acids and essential oils （OA/EO）. The feed additive tested was provided by Jefo Nutrition Inc., St-Hyacinthe, Quebec, Canada. Day-old birds were separated into two rooms and subdivided into two groups. Chicken were fed with OA/EO or not fed with OA/EO until they reached 35 d of age. At 14 d of age, chickens received an oral suspension of two well characterized C. jejuni strains, depending on the room they were housed in. The levels of C. jejuni were periodically monitored in the caecum and on the carcasses. C. jejuni colonization was further characterized by the use of high-resolution melt analysis of the C. jejuniflaA gene （HRM-flaA）. The effect of the feed additive was strain-dependent. In room two, the feed additive had no effect on the caecal counts. In room one, at 35 d of age, caecal C. jejuni counts were higher with OA/EO, as opposed to carcasses counts which were lower in the treated group. The HRM-flaA analysis showed that an amplification profile was predominant in birds fed with OA/EO at 35 d of age in room one, suggesting the selection of a C. jejuni strain. In conclusion, the OA/EO seemed to be effective to reduce C. jejuni levels but this effect appeared strain dependent.
文摘Campylobacter jejuni infection has been implicated in the pathogenesis of Guillain-Barré syndrome (GBS) due to production of humoral immune response against neural antigens. A case-control study was performed in a tertiary care teaching hospital for the estimation of anti-ganglioside antibodies in GBS patients and their controls. Blood samples were collected from 59 GBS cases, 58 neurological controls (NC) and 60 non-neurological control (NNC) patients for automatic estimation of IgG and IgM antibodies to seven gangliosides using EUROLineScan software. Antibodies of IgG class for GM1 were highly significant in GBS (p = 0.000) and NC (p = 0.031) compared to NNC. However GBS group was not significant (p = 0.413) compared to NC. For GM2 ganglioside, GBS and NC groups were significant (p = 0.000) compared to NNC, but GBS group was not significant (p = 0.999) compared to NC. For GM3 ganglioside, GBS and NC groups were significant (p = 0.000) compared to NNC;but GBS group was insignificant (p = 0.858) compared to NC with similar trend for all other ganglioside antibodies. When IgM class of antibodies was evaluated for GM1, GBS group was not significant (p = 0.604) whereas NC group was significant (p = 0.000) compared to NNC. GBS group was not significant compared to NC (p = 0.011). The trend was the same for GM2 antibodies. For GM3, GBS group was significant (p = 0.010) and NC was near significant (p = 0.055) compared to NNC. However GBS group was not significant (p = 0.808) compared to NC. No groups were significant (p > 0.05) in relation to the remaining gangliosides except for GQ1b where GBS group (p = 0.001) and NC group were significant (p = 0.000) compared to NNC. GBS group was also significant (p = 0.001) compared to NC and NNC. Anti-gangliosides antibodies were present in highly significant levels in the GBS group, though they were also present in the non-paralytic neurological control patients compared to the non-neurological control group.
