Cross-talk between microRNA-let7c and transforming growth factor-β2 during epithelial-to-mesenchymal transition of retinal pigment epithelial cells

AIM: To explore the roles of microRNA-let7 c(miR-let7 c) and transforming growth factor-β2(TGF-β2) and cellular signaling during epithelial-to-mesenchymal transition(EMT) of retinal pigment epithelial cells. METHODS: Retinal pigment epithelial(ARPE-19) cells were cultured with no serum for 12 h, and then with recombinant human TGF-β2 for different lengths of time. ARPE-19 cells were transfected with 1×106 TU/mL miR-let7 c mimcs(miR-let7 cM), miR-let7 c mimcs negative control(miR-let7cMNC) and miR-let7 c inhibitor(miR-let7 cI) using the transfection reagent. The expression of keratin-18, vimentin, N-cadherin, IKB alpha, p65 were detected by Western blot, quantitative polymerase chain reaction and immunofluorescence. RESULTS: The expression of miR-let7c was dramatically reduced and the nuclear factor-kappa B(NF-κB) signaling pathway was activated after induction by TGF-β2(P<0.05). In turn, overexpressed miR-let7 c significantly inhibited TGF-β2-induced EMT(P<0.05). However, miR-let7 c was unable to inhibit TGF-β2-induced EMT when the NF-κB signaling pathway was inhibited by BAY11-7082(P<0.01). CONCLUSION: The miR-let7 c regulates TGF-β2-induced EMT through the NF-κB signaling pathway in ARPE-19 cells.

C.I.颜料绿7苯甲酸颜料化工艺研究

用苯甲酸及其烷基衍生物对酞菁绿粗品进行颜料化,制备不含添加剂的C. I.颜料绿7颜料。考察了苯甲酸及其烷基衍生物不同组分处理效果,并进行优化。结果表明,在温度100~105℃、苯甲酸/颜料质量比0.8、时间6 h的最优化工艺条件下,C. I.颜料绿7商品收率约99%,苯甲酸回收率达95%,与现有工艺GF311商品相比,在水性涂料中,它的着色力为111.22%,色光为+0.05,饱和度为+2.0。

C.I.颜料绿7重结晶颜料化工艺

对C.I.颜料绿7进行了溶剂法重结晶颜料化工艺研究,并考察了溶剂、温度及时间等条件对重结晶颜料粗品的影响。最佳工艺条件为:邻乙基苯甲酸作溶剂、溶剂与颜料比1∶1、反应温度100℃,时间7 h,再经水解、过滤、洗涤、干燥处理。结果显示,该工艺得到的颜料与现有工艺GF311商品相比,水性应用着色力为111.04%,饱和度为+2.18。邻乙基苯甲酸回收率达95%。与传统芳烃-松香处理工艺相比,颜料纯度高,不含添加剂,利于下游客户使用和再加工。