Objective Atherosclerotic lesions preferentially occur at branch points of arterial trees where the blood flow is disturbed.Disturbed flow increases endothelial permeability,vascular barrier dysfunction,and finally th...Objective Atherosclerotic lesions preferentially occur at branch points of arterial trees where the blood flow is disturbed.Disturbed flow increases endothelial permeability,vascular barrier dysfunction,and finally the development of atherosclerosis.CTRP1,a member of C1q/TNF related protein(CTRP)family,is a novel secreted glycoprotein and its biological functions are largely undefined.展开更多
C1q is the first subcomponent of classical pathway in the complement system and a major link between innate and acquired immunities. The globular （gC1q） domain similar with C1q was also found in many non-complement ...C1q is the first subcomponent of classical pathway in the complement system and a major link between innate and acquired immunities. The globular （gC1q） domain similar with C1q was also found in many non-complement C1q-domain-containing （C1qDC） proteins which have similar crystal structure to that of the multifunctional tumor necrosis factor （TNF） ligand family, and also have diverse functions. In this study, we identified a total of 52 independent gene sequences encoding C1q-domain-containing proteins through comprehensive searches of zebrafish genome, cDNA and EST databases. In comparison to 31 orthologous genes in human and different numbers in other species, a significant selective pressure was suggested during vertebrate evolution. Domain organization of C1q-domain-containing （C1qDC） proteins mainly includes a leading signal peptide, a collagen-like region of variable length, and a C-terminal C1q domain. There are 11 highly conserved residues within the C1q domain, among which 2 are invariant within the zebrafish gene set. A more extensive database searches also revealed homologous C1qDC proteins in other vertebrates, invertebrates and even bacterium, but no homologous sequences for encoding C1qDC proteins were found in many species that have a more recent evolutionary history with zebrafish. Therefore, further studies on C1q-domain-containing genes among different species will help us understand evolutionary mechanism of innate and acquired immunities.展开更多
目的探讨2型糖尿病（T2DM）患者血清C1q／TNF相关蛋白3（C1q tumor necrosis factor related protein 3,CTRP3），C1q／TNF相关蛋白9（C1q tumor necrosis factor related protein 9 ,CTRP9）与动脉粥样硬化病变（atherosclerosis，AS...目的探讨2型糖尿病（T2DM）患者血清C1q／TNF相关蛋白3（C1q tumor necrosis factor related protein 3,CTRP3），C1q／TNF相关蛋白9（C1q tumor necrosis factor related protein 9 ,CTRP9）与动脉粥样硬化病变（atherosclerosis，AS）的相关性。方法随机选取2014年1月-2015年1月陕西省人民医院内分泌科住院的成人新发T2DM患者196例，入选T2DM组，并根据颈动脉彩色多普勒超声结果将其分为糖尿病伴动脉粥样硬化组（AS组，7l例）和单纯糖尿病组（非AS组，125例），另外随机选取同期健康体检者152例作为正常对照组，测定所有受试者的体重指数（BMI），腰围（WC），臀围（HIP），腰臀比（WHR），收缩压（SBP），舒张压（DBP），空腹血糖（FBG），餐后2h血糖（2hPBG），糖化血红蛋白（HBA1c），三酰甘油（TG），总胆固醇（TC），高密度脂蛋白胆固醇（HDL-C），低密度脂蛋白胆固醇（LDL-C），CTRP3及CTRP9。结果①T2DM组血清与正常对照组比较CTRP3（0．67±0．34ng／ml vs 0.99±0．32ng／ml），CTRP9（9．04±3．11ng／ml vs 12. 11±2.89 ng/ml）, HDL-C（ 1.48 ± 0.27 mmol/L vs 1.23±0.57 mmol/L）, FBG （ 4.62 ± 0.53 mmol/L vs 9.41 ± 2. 61 mmol/L）,2hPBG（5. 90±0. 80 mmol/L vs 11.55±3.20 mmol/L）,HBA1c（4.86±0.45 mmol/L vs 8.79±2.14 mmol/L）, LDL-C（2. 48±0. 36 mmol/L vs 3.58±0. 89 mmol/L）,TC（3.64±1. 10 mmol/L vs 5.77±0.97 mmol/L）,TG（1.23±0.34 mmol/L vs 1.71±0.75 mmol/L）差异有统计学显著性意义（t=-13．069-5．88，P值均〈0．01）。②AS组与非AS组比较CTRP3（O．67±0．34ng／ml vs 0．99±0．32ng／m1），CTRP9（9．04±3．11ng／ml vs 12．11±2．89ng／m1），年龄（55．82±8．80岁vs 62．49±6．54岁）差异有统计学显著性意义（t／χ^2=0．036-7．345，P值均〈0．01），两组血清TC（5．61±0．90mmol／L vs 6．05±1．02mmol／L），LDL-C（3．44±0．80mmol／L vs3．83±1．01mmol／L）差异亦有统计学显著性意义（t=-2．083--2．197，P值均〈0．05）。③相关性研究：CTRP3与WC（r=-0．932），DBP（r=-O．932），FBG（r=-0．856），TG（r=-0．728），TC（r=-0．920）呈负相关（P值均〈0．01），CTRP9和年龄（r=-0．958）呈负相关（P值均〈0．01），与HDL-C（r=0．860）呈正相关（P〈0．01）。④多元逐步回归分析：WC，DBP，FBG是CTRP3的独立危险因子（P〈0．01），年龄，HDL-C是CTRP9的独立危险因子（P〈0．01）。结论CTRP3，CTRP9可能具有抗动脉粥样硬化作用，是糖尿病动脉粥样硬化的保护因子。展开更多
文摘Objective Atherosclerotic lesions preferentially occur at branch points of arterial trees where the blood flow is disturbed.Disturbed flow increases endothelial permeability,vascular barrier dysfunction,and finally the development of atherosclerosis.CTRP1,a member of C1q/TNF related protein(CTRP)family,is a novel secreted glycoprotein and its biological functions are largely undefined.
基金Acknowledgements This work was supported by the National Major Basic Research Program （No. 2004CB117401）；by the National Natural Science Foundation of China （No. 90408013）.
文摘C1q is the first subcomponent of classical pathway in the complement system and a major link between innate and acquired immunities. The globular （gC1q） domain similar with C1q was also found in many non-complement C1q-domain-containing （C1qDC） proteins which have similar crystal structure to that of the multifunctional tumor necrosis factor （TNF） ligand family, and also have diverse functions. In this study, we identified a total of 52 independent gene sequences encoding C1q-domain-containing proteins through comprehensive searches of zebrafish genome, cDNA and EST databases. In comparison to 31 orthologous genes in human and different numbers in other species, a significant selective pressure was suggested during vertebrate evolution. Domain organization of C1q-domain-containing （C1qDC） proteins mainly includes a leading signal peptide, a collagen-like region of variable length, and a C-terminal C1q domain. There are 11 highly conserved residues within the C1q domain, among which 2 are invariant within the zebrafish gene set. A more extensive database searches also revealed homologous C1qDC proteins in other vertebrates, invertebrates and even bacterium, but no homologous sequences for encoding C1qDC proteins were found in many species that have a more recent evolutionary history with zebrafish. Therefore, further studies on C1q-domain-containing genes among different species will help us understand evolutionary mechanism of innate and acquired immunities.
文摘目的探讨2型糖尿病（T2DM）患者血清C1q／TNF相关蛋白3（C1q tumor necrosis factor related protein 3,CTRP3），C1q／TNF相关蛋白9（C1q tumor necrosis factor related protein 9 ,CTRP9）与动脉粥样硬化病变（atherosclerosis，AS）的相关性。方法随机选取2014年1月-2015年1月陕西省人民医院内分泌科住院的成人新发T2DM患者196例，入选T2DM组，并根据颈动脉彩色多普勒超声结果将其分为糖尿病伴动脉粥样硬化组（AS组，7l例）和单纯糖尿病组（非AS组，125例），另外随机选取同期健康体检者152例作为正常对照组，测定所有受试者的体重指数（BMI），腰围（WC），臀围（HIP），腰臀比（WHR），收缩压（SBP），舒张压（DBP），空腹血糖（FBG），餐后2h血糖（2hPBG），糖化血红蛋白（HBA1c），三酰甘油（TG），总胆固醇（TC），高密度脂蛋白胆固醇（HDL-C），低密度脂蛋白胆固醇（LDL-C），CTRP3及CTRP9。结果①T2DM组血清与正常对照组比较CTRP3（0．67±0．34ng／ml vs 0.99±0．32ng／ml），CTRP9（9．04±3．11ng／ml vs 12. 11±2.89 ng/ml）, HDL-C（ 1.48 ± 0.27 mmol/L vs 1.23±0.57 mmol/L）, FBG （ 4.62 ± 0.53 mmol/L vs 9.41 ± 2. 61 mmol/L）,2hPBG（5. 90±0. 80 mmol/L vs 11.55±3.20 mmol/L）,HBA1c（4.86±0.45 mmol/L vs 8.79±2.14 mmol/L）, LDL-C（2. 48±0. 36 mmol/L vs 3.58±0. 89 mmol/L）,TC（3.64±1. 10 mmol/L vs 5.77±0.97 mmol/L）,TG（1.23±0.34 mmol/L vs 1.71±0.75 mmol/L）差异有统计学显著性意义（t=-13．069-5．88，P值均〈0．01）。②AS组与非AS组比较CTRP3（O．67±0．34ng／ml vs 0．99±0．32ng／m1），CTRP9（9．04±3．11ng／ml vs 12．11±2．89ng／m1），年龄（55．82±8．80岁vs 62．49±6．54岁）差异有统计学显著性意义（t／χ^2=0．036-7．345，P值均〈0．01），两组血清TC（5．61±0．90mmol／L vs 6．05±1．02mmol／L），LDL-C（3．44±0．80mmol／L vs3．83±1．01mmol／L）差异亦有统计学显著性意义（t=-2．083--2．197，P值均〈0．05）。③相关性研究：CTRP3与WC（r=-0．932），DBP（r=-O．932），FBG（r=-0．856），TG（r=-0．728），TC（r=-0．920）呈负相关（P值均〈0．01），CTRP9和年龄（r=-0．958）呈负相关（P值均〈0．01），与HDL-C（r=0．860）呈正相关（P〈0．01）。④多元逐步回归分析：WC，DBP，FBG是CTRP3的独立危险因子（P〈0．01），年龄，HDL-C是CTRP9的独立危险因子（P〈0．01）。结论CTRP3，CTRP9可能具有抗动脉粥样硬化作用，是糖尿病动脉粥样硬化的保护因子。
