Environmental stress factors could lead to the excess generation of reactive oxygen species（ROS） that induces various forms of skin damage related to oxidative stress. Polyhydroxylated fullerene derivative C（60）...Environmental stress factors could lead to the excess generation of reactive oxygen species（ROS） that induces various forms of skin damage related to oxidative stress. Polyhydroxylated fullerene derivative C（60）（OH）n, acting as an effective agent for prevention of skin aging, is widely used in the lotion and sunscreens in the field of cosmetics, but rarely used in the masks. In this study, we prepared C（60）（OH）n loaded nanofibrous membranes to protect human keratinocyte cells from ROS-associated damage and suppress the elevation of intracellular ROS and Ca（2＋） along with the apoptotic cell death. Two FDAapproved biodegradable polymers, PLGA and PCL, have been used for making the electrospun nanofibers,with C（60）（OH）n added to the polymers as an antioxidant. The nanofibrous membranes with good biocompatibility might be potentially applied in clinical practice to reduce skin aging.展开更多
基金financially supported by the project Electro Med(No.11-115313) from the Danish Council for Strategic Researchthe National Science Fund for Excellent Young Scholars(No.31622026)+2 种基金the National Natural Science Foundation of China(Nos.U1532122,11435002,21471044)the National Key Research and Development Plan(Nos.2016YFA0201600,2016YFA0203204)CAS Youth Innovation Promotion Association(No.2014031)
文摘Environmental stress factors could lead to the excess generation of reactive oxygen species（ROS） that induces various forms of skin damage related to oxidative stress. Polyhydroxylated fullerene derivative C（60）（OH）n, acting as an effective agent for prevention of skin aging, is widely used in the lotion and sunscreens in the field of cosmetics, but rarely used in the masks. In this study, we prepared C（60）（OH）n loaded nanofibrous membranes to protect human keratinocyte cells from ROS-associated damage and suppress the elevation of intracellular ROS and Ca（2＋） along with the apoptotic cell death. Two FDAapproved biodegradable polymers, PLGA and PCL, have been used for making the electrospun nanofibers,with C（60）（OH）n added to the polymers as an antioxidant. The nanofibrous membranes with good biocompatibility might be potentially applied in clinical practice to reduce skin aging.
