目的:探讨原发性高血压家族成员的G蛋白β3亚单位C825T基因多态性与代谢综合征的关系。方法:应用PCR和PCR-限制性片段长度多态性技术(PCR-restriction fragment length polymor-phism,PCR-RFLP)对原发性高血压家族成员中的148例代谢综...目的:探讨原发性高血压家族成员的G蛋白β3亚单位C825T基因多态性与代谢综合征的关系。方法:应用PCR和PCR-限制性片段长度多态性技术(PCR-restriction fragment length polymor-phism,PCR-RFLP)对原发性高血压家族成员中的148例代谢综合征患者(代谢综合征组)和105例非代谢综合征者(对照组)进行G蛋白β3亚单位C825T基因多态性分析。结果:T等位基因频率在代谢综合征组显著高于对照组(57%比41%,P(0·05)。回归分析结果显示,调整其他危险因素影响后,825CT/TT基因型与代谢综合征呈正相关。结论:G蛋白β3亚单位C825T基因多态性与原发性高血压家族成员的代谢综合征发病有关,T等位基因可能是原发性高血压家族成员发生代谢综合征的遗传危险因素之一。展开更多
Background/Aims: The outcome of infection with the hepatitis C virus (HCV) has been shown to be influenced by genetic host factors. The G protein β 3 subunit (GNB3) C825T polymorphism has been shown to determine immu...Background/Aims: The outcome of infection with the hepatitis C virus (HCV) has been shown to be influenced by genetic host factors. The G protein β 3 subunit (GNB3) C825T polymorphism has been shown to determine immune cell functions in vitro. We investigated the association of GNB3 genotypes with treatment response in HCV- infected patients. Methods: We genotyped 1781 HCV- free blood donors and 232 HCV- infected patients treated with interferon- alfa/ ribavirin. Sustained virologic response (SVR) was defined by undetectable HCVRNA 24 weeks after discontinuation of therapy. Non- response (NR) was defined by positive HCV- RNA at the end of at least 24 weeks of treatment. GNB3 genotypes were determined by DNA restriction enzyme analyses. Results: Genotype distribution was not significantly different in healthy controls and HCV- infected patients. Only in HCV genotype 1- infected patients a significant correlation between GNB3 CC genotype and NR could be observed (6 TT, 42 TC, 54 CC) versus SVR (11 TT, 25 TC, 19 CC) patients (P=0.004). In a logistic regression analysis including biochemical and virologic characteristics, only GNB3 CC genotype was significantly associated with NR (OR 4.9; 95% CI=1.4- 16.5; P=0.011). Conclusions: The GNB3 825 CC genotype is associated with NR in HCV- 1- infected patients.展开更多
Background A polymorphism consisting of a C825T substitution in the G protein β3 subunit gene (GNB3) has been associated with enhanced human atrial inward r ectifier potassium currents regarding the TT genotype. Ther...Background A polymorphism consisting of a C825T substitution in the G protein β3 subunit gene (GNB3) has been associated with enhanced human atrial inward r ectifier potassium currents regarding the TT genotype. Therefore, we investigate d a possible impact of the GNB3 C825T polymorphismon atrial fibrillation in an a ssociation study. Methods Two hundred ninety one consecutive patients admitted to our center with atrial fibrillation (age, 58±10 years) and 292 consecutive c ontrol patients without atrial tachyarrhythmias (59±11 years) were genotyped fo r the C825T polymorphism. Patients with coronary heart disease, valvular heart d isease, or cardiomyopathy were excluded from the study. Both patient groups had a similar incidence of cardiovascular risk factors (hypertension, hypercholester olemia, body mass index). Results The prevalence of the GNB3 TT genotype was sig nificantly lower in patients with atrial fibrillation(5.8%) than in the control group(12.0%); however, no significant differences in the frequencies of the CT and CC genotypes were found. The TT genotype was associated with a 54%decrease in the adjusted risk (OR from a multivariant model, 0.46; 95%CI, 0.24 to 0.87; P=.02) for the occurrence of atrial fibrillation. Conclusions The current study suggests an association between the GNB3 TT genotype and a reduced risk for the occurrence of atrial fibrillation.展开更多
文摘目的:探讨原发性高血压家族成员的G蛋白β3亚单位C825T基因多态性与代谢综合征的关系。方法:应用PCR和PCR-限制性片段长度多态性技术(PCR-restriction fragment length polymor-phism,PCR-RFLP)对原发性高血压家族成员中的148例代谢综合征患者(代谢综合征组)和105例非代谢综合征者(对照组)进行G蛋白β3亚单位C825T基因多态性分析。结果:T等位基因频率在代谢综合征组显著高于对照组(57%比41%,P(0·05)。回归分析结果显示,调整其他危险因素影响后,825CT/TT基因型与代谢综合征呈正相关。结论:G蛋白β3亚单位C825T基因多态性与原发性高血压家族成员的代谢综合征发病有关,T等位基因可能是原发性高血压家族成员发生代谢综合征的遗传危险因素之一。
文摘Background/Aims: The outcome of infection with the hepatitis C virus (HCV) has been shown to be influenced by genetic host factors. The G protein β 3 subunit (GNB3) C825T polymorphism has been shown to determine immune cell functions in vitro. We investigated the association of GNB3 genotypes with treatment response in HCV- infected patients. Methods: We genotyped 1781 HCV- free blood donors and 232 HCV- infected patients treated with interferon- alfa/ ribavirin. Sustained virologic response (SVR) was defined by undetectable HCVRNA 24 weeks after discontinuation of therapy. Non- response (NR) was defined by positive HCV- RNA at the end of at least 24 weeks of treatment. GNB3 genotypes were determined by DNA restriction enzyme analyses. Results: Genotype distribution was not significantly different in healthy controls and HCV- infected patients. Only in HCV genotype 1- infected patients a significant correlation between GNB3 CC genotype and NR could be observed (6 TT, 42 TC, 54 CC) versus SVR (11 TT, 25 TC, 19 CC) patients (P=0.004). In a logistic regression analysis including biochemical and virologic characteristics, only GNB3 CC genotype was significantly associated with NR (OR 4.9; 95% CI=1.4- 16.5; P=0.011). Conclusions: The GNB3 825 CC genotype is associated with NR in HCV- 1- infected patients.
文摘Background A polymorphism consisting of a C825T substitution in the G protein β3 subunit gene (GNB3) has been associated with enhanced human atrial inward r ectifier potassium currents regarding the TT genotype. Therefore, we investigate d a possible impact of the GNB3 C825T polymorphismon atrial fibrillation in an a ssociation study. Methods Two hundred ninety one consecutive patients admitted to our center with atrial fibrillation (age, 58±10 years) and 292 consecutive c ontrol patients without atrial tachyarrhythmias (59±11 years) were genotyped fo r the C825T polymorphism. Patients with coronary heart disease, valvular heart d isease, or cardiomyopathy were excluded from the study. Both patient groups had a similar incidence of cardiovascular risk factors (hypertension, hypercholester olemia, body mass index). Results The prevalence of the GNB3 TT genotype was sig nificantly lower in patients with atrial fibrillation(5.8%) than in the control group(12.0%); however, no significant differences in the frequencies of the CT and CC genotypes were found. The TT genotype was associated with a 54%decrease in the adjusted risk (OR from a multivariant model, 0.46; 95%CI, 0.24 to 0.87; P=.02) for the occurrence of atrial fibrillation. Conclusions The current study suggests an association between the GNB3 TT genotype and a reduced risk for the occurrence of atrial fibrillation.
