Objective:To evaluate the consequence of oral administration of Calliandra portoricensis(C. portoricensis) leaf extract on the stomach and pancreas in Swiss albino mice.Methods:Three, groups of mice(B,C and D) were tr...Objective:To evaluate the consequence of oral administration of Calliandra portoricensis(C. portoricensis) leaf extract on the stomach and pancreas in Swiss albino mice.Methods:Three, groups of mice(B,C and D) were treated with 4 mg/kg of C.portoricensis extract.Croup A was the control and received an equivalent volume of distilled water.Group B received C.portoricensis leaf extract for 7 days.Croup C received C.portoricensis leaf extract for 14 days,and Croup D received C.portoricensis leaf extract for 28 days.At different stages in the study,the mice were sacrificed and the stomach and pancreas were excised and fixed in 10%formol saline for histological analysis.Results:The result showed a normal microstructural outline in groups B and C as compared with the control.However,animals in group D showed disorganization of the mucosa and discontinuation of epithelial lining of the stomach while the islets of Langerans in the pancreas were at various degree of degeneration as compared with the control mice. Conclusions:The present finding suggests that chronic administration(28 days as seen in this study) of C.portoricensis leaf extract may inhibit the proper function of the stomach and pancreas.展开更多
Objective:To assess ovicidal activity of three acetonic extracts from the leaves of three forage legume,Calliandra calotyrsus(C.calotyrsus),Gliricidia sepium(G.sepium) and Leucaena diversifolia(L.diversifolia) in vitr...Objective:To assess ovicidal activity of three acetonic extracts from the leaves of three forage legume,Calliandra calotyrsus(C.calotyrsus),Gliricidia sepium(G.sepium) and Leucaena diversifolia(L.diversifolia) in vitro on Haemonchus contortus(H.contortus).Methods:Eggs were exposed for 24 hours to five different concentrations(0.075,0.15,0.3,0.6 and 1.25 mg/mL) of acetonic extracts at room temperature(24℃).Distilled water and 0.4%Tween were used in the bioassay as negative controls.Results:The later did not affect embryonation and egg hatching of H.contortus.Conversively,significant effects were obtained with the acetonic extracts of leaves of all three plants and the maximum activity was observed with the highest concentration (1.25 mg/mL).The acetonic extract of G.sepium was found to be more active(2.9%and 0.0% for embryonation and egg hatching,respectively) than the other substances 16.5%and 33.5%, respectively for C.calothyrsus,33.7%and 33.3%,respectively for L diversifolia.Conclusions: These results suggest that the three forage legumes do possess ovicidal properties and further studies on larvae should be carried out.展开更多
OBJECTIVE： Prostate cancer（PCa） is a major health concern. Calliandra portoricensis（CP） is traditionally known for its analgesic, anti-ulcerogenic and anticonvulsant properties. However, its antiproliferative pro...OBJECTIVE： Prostate cancer（PCa） is a major health concern. Calliandra portoricensis（CP） is traditionally known for its analgesic, anti-ulcerogenic and anticonvulsant properties. However, its antiproliferative properties for PCa still need to be investigated. METHODS： Antioxidant activities of CP were determined by 1,1-diphenyl-2-picryhydrazyl（DPPH） and hydroxyl（OH-） radicals-scavenging methods. PC-3 and LNCa P（androgen-refractory and androgendependent PCa-derived cell lines） were cultured and treated with CP（10, 50 and 100 μg/m L）. Effects of CP on cells were determined by cytotoxicity assay（lactate dehydrogenase, LDH） and viability assay（sodium 3′-[1-（phenylaminocarbonyl）-3,4-tetrazolium]-bis（4-methoxy-6-nitro） benzene sulfonic acid hydrate, XTT）. DNA fragmentation was detected by cell death detection enzyme-linked immunosorbent assay plus kit. CP was tested as an inhibitor of angiogenesis using chicken chorioallantoic membrane（CAM） assay. RESULTS： CP showed significant scavenging of DPPH and OH- radicals. CP significantly（P〈0.05） inhibited lipid peroxidation in a dose-dependent manner. Precisely, CP（10, 50 and 100 μg/m L） inhibited PC-3 and LNCa P growth by 7%, 74% and 92%, and 27%, 73%, and 85% respectively at 48 h. CP had low toxicity in vitro at its half inhibitory concentration dose. Detection of cell death induced by CP at 50 μg/m L showed higher enrichment factors in LNCa P（7.38±0.95） than PC-3（3.48±0.55）. Also, treatment with CP（50 μg/m L） significantly reduced network of vessels in CAM, suggesting its antiangiogenic potential. CONCLUSION： Calliandra portoricensis elicited antioxidant, antiangiogenic and antiproliferative effects in PCa cells.展开更多
文摘Objective:To evaluate the consequence of oral administration of Calliandra portoricensis(C. portoricensis) leaf extract on the stomach and pancreas in Swiss albino mice.Methods:Three, groups of mice(B,C and D) were treated with 4 mg/kg of C.portoricensis extract.Croup A was the control and received an equivalent volume of distilled water.Group B received C.portoricensis leaf extract for 7 days.Croup C received C.portoricensis leaf extract for 14 days,and Croup D received C.portoricensis leaf extract for 28 days.At different stages in the study,the mice were sacrificed and the stomach and pancreas were excised and fixed in 10%formol saline for histological analysis.Results:The result showed a normal microstructural outline in groups B and C as compared with the control.However,animals in group D showed disorganization of the mucosa and discontinuation of epithelial lining of the stomach while the islets of Langerans in the pancreas were at various degree of degeneration as compared with the control mice. Conclusions:The present finding suggests that chronic administration(28 days as seen in this study) of C.portoricensis leaf extract may inhibit the proper function of the stomach and pancreas.
文摘Objective:To assess ovicidal activity of three acetonic extracts from the leaves of three forage legume,Calliandra calotyrsus(C.calotyrsus),Gliricidia sepium(G.sepium) and Leucaena diversifolia(L.diversifolia) in vitro on Haemonchus contortus(H.contortus).Methods:Eggs were exposed for 24 hours to five different concentrations(0.075,0.15,0.3,0.6 and 1.25 mg/mL) of acetonic extracts at room temperature(24℃).Distilled water and 0.4%Tween were used in the bioassay as negative controls.Results:The later did not affect embryonation and egg hatching of H.contortus.Conversively,significant effects were obtained with the acetonic extracts of leaves of all three plants and the maximum activity was observed with the highest concentration (1.25 mg/mL).The acetonic extract of G.sepium was found to be more active(2.9%and 0.0% for embryonation and egg hatching,respectively) than the other substances 16.5%and 33.5%, respectively for C.calothyrsus,33.7%and 33.3%,respectively for L diversifolia.Conclusions: These results suggest that the three forage legumes do possess ovicidal properties and further studies on larvae should be carried out.
基金supported by a 6-month grant given to OA by the Bank-Anthony Charitable Trust Will (UK) which was disbursed by the College of Medicine, University of Ibadan, Nigeria, Charite University of Medicine, Berlin and Urologic Research Foundation Berlin, Germany
文摘OBJECTIVE： Prostate cancer（PCa） is a major health concern. Calliandra portoricensis（CP） is traditionally known for its analgesic, anti-ulcerogenic and anticonvulsant properties. However, its antiproliferative properties for PCa still need to be investigated. METHODS： Antioxidant activities of CP were determined by 1,1-diphenyl-2-picryhydrazyl（DPPH） and hydroxyl（OH-） radicals-scavenging methods. PC-3 and LNCa P（androgen-refractory and androgendependent PCa-derived cell lines） were cultured and treated with CP（10, 50 and 100 μg/m L）. Effects of CP on cells were determined by cytotoxicity assay（lactate dehydrogenase, LDH） and viability assay（sodium 3′-[1-（phenylaminocarbonyl）-3,4-tetrazolium]-bis（4-methoxy-6-nitro） benzene sulfonic acid hydrate, XTT）. DNA fragmentation was detected by cell death detection enzyme-linked immunosorbent assay plus kit. CP was tested as an inhibitor of angiogenesis using chicken chorioallantoic membrane（CAM） assay. RESULTS： CP showed significant scavenging of DPPH and OH- radicals. CP significantly（P〈0.05） inhibited lipid peroxidation in a dose-dependent manner. Precisely, CP（10, 50 and 100 μg/m L） inhibited PC-3 and LNCa P growth by 7%, 74% and 92%, and 27%, 73%, and 85% respectively at 48 h. CP had low toxicity in vitro at its half inhibitory concentration dose. Detection of cell death induced by CP at 50 μg/m L showed higher enrichment factors in LNCa P（7.38±0.95） than PC-3（3.48±0.55）. Also, treatment with CP（50 μg/m L） significantly reduced network of vessels in CAM, suggesting its antiangiogenic potential. CONCLUSION： Calliandra portoricensis elicited antioxidant, antiangiogenic and antiproliferative effects in PCa cells.
