Objective: To explore the mechanism of Ningshenling Granule in the treatment of depression. Methods: The rat model of depression was established, and Ningshenling Granule was given orally. The morphological changes of...Objective: To explore the mechanism of Ningshenling Granule in the treatment of depression. Methods: The rat model of depression was established, and Ningshenling Granule was given orally. The morphological changes of hippocampus were observed by HE staining, and the changes of dopamine receptor D2 and CAMK2a mRNA were observed by RT-pcr. Result:Ningshenling group could significantly improve the morphological changes of pyramidal cells in hippocampus and the expressions of D2 and CAMK2a in brain tissue. Conclusion:Ningshenling can treat depression, and its mechanism is related to regulating the expression of related factors in the brain.展开更多
Objective:To explore the mechanism of Ningshenling Granule in the treatment of depression.Methods:The rat model of depression was established,and Ningshenling Granule was given orally.The morphological changes of hipp...Objective:To explore the mechanism of Ningshenling Granule in the treatment of depression.Methods:The rat model of depression was established,and Ningshenling Granule was given orally.The morphological changes of hippocampus were observed by HE staining,and the changes of dopamine receptor D2 and CAMK2a mRNA were observed by RT-pcr.Result:Ningshenling group could significantly improve the morphological changes of pyramidal cells in hippocampus and the expressions of D2 and CAMK2a in brain tissue.Conclusions:Ningshenling can treat depression,and its mechanism is related to regulating the expression of related factors in the brain.展开更多
目的探讨微小核糖核苷酸-219（microRNA-219，miR-219）及其靶基因钙离子／钙调蛋白依赖性蛋白激酶Ⅱγ（Ca2＋／calmodulin-dependent protein kinaseⅡ gamma，CAMK2G）多态性与精神分裂症的关系。方法采用TaqMan探针基因分型技术在...目的探讨微小核糖核苷酸-219（microRNA-219，miR-219）及其靶基因钙离子／钙调蛋白依赖性蛋白激酶Ⅱγ（Ca2＋／calmodulin-dependent protein kinaseⅡ gamma，CAMK2G）多态性与精神分裂症的关系。方法采用TaqMan探针基因分型技术在中国汉族人群中对1041例精神分裂症患者和953例正常对照者进行miR-219基因rs107822和CAMK2G基因rs2306327的分型。利用二级结构预测软件CentroidFold预测rsl07822对miR．219二级结构影响。结果miR-219基因rs107822多态的等位基因分布在患者组与正常对照组之间差异有统计学意义（X2=7．33，P=0．007）；在非条件Logistic回归分析中，rs107822基因型分布最符合加性遗传模式（P=0．0072），携带C等位基因可降低精神分裂症的发生风险（OR=O．84，95％CI=O．74～0．95）；软件预测发现rs107822T等位基因突变成C等位基因后，miR-219基因的初级转录产物的二级结构发生了改变。CAMK2G基因多态性rs2306327的等位基因和基因型分布在病例组与对照组间的差异均无统计学意义（均P〉0．05）。结论在中国汉族人群中，miR-219基因rs107822多态可能与精神分裂症有关。展开更多
Many of the effects of Ca^2＋ signaling are mediated through the Ca^2＋/calmodulin complex and its acceptors, the Ca^2＋/calmodulin-dependent protein kinases, including PSKHI. Studies of the proteins involved in the c...Many of the effects of Ca^2＋ signaling are mediated through the Ca^2＋/calmodulin complex and its acceptors, the Ca^2＋/calmodulin-dependent protein kinases, including PSKHI. Studies of the proteins involved in the calcium metabolism in oysters will help elucidate the pearl formation mechanism. This paper describes a full-length PSKH1 cDNA isolated from pearl oyster Pinctada fucata. Oyster PSKH1 shares 65% homology with human PSKH1 and 48% similarity with rat CaM kinase I in the amino acid sequence, and contains a calmodulin-binding domain. The results of semi-quantitative reverse transcription-polymerase chain reaction and in situ hybridization revealed that oyster PSKH1 mRNA is highly expressed in the outer epithelial cells of the mantle pallial and in the gill epithelial cells. These studies provide important information describing the complex Ca^2＋ signaling mechanism in oyster calcium metabolism.展开更多
文摘Objective: To explore the mechanism of Ningshenling Granule in the treatment of depression. Methods: The rat model of depression was established, and Ningshenling Granule was given orally. The morphological changes of hippocampus were observed by HE staining, and the changes of dopamine receptor D2 and CAMK2a mRNA were observed by RT-pcr. Result:Ningshenling group could significantly improve the morphological changes of pyramidal cells in hippocampus and the expressions of D2 and CAMK2a in brain tissue. Conclusion:Ningshenling can treat depression, and its mechanism is related to regulating the expression of related factors in the brain.
基金Natural science foundation of heilongjiang province(No.H2017043)Harbin applied technology and development project excellent academic leader program(No.2017RAXQJ069).
文摘Objective:To explore the mechanism of Ningshenling Granule in the treatment of depression.Methods:The rat model of depression was established,and Ningshenling Granule was given orally.The morphological changes of hippocampus were observed by HE staining,and the changes of dopamine receptor D2 and CAMK2a mRNA were observed by RT-pcr.Result:Ningshenling group could significantly improve the morphological changes of pyramidal cells in hippocampus and the expressions of D2 and CAMK2a in brain tissue.Conclusions:Ningshenling can treat depression,and its mechanism is related to regulating the expression of related factors in the brain.
文摘目的探讨微小核糖核苷酸-219（microRNA-219，miR-219）及其靶基因钙离子／钙调蛋白依赖性蛋白激酶Ⅱγ（Ca2＋／calmodulin-dependent protein kinaseⅡ gamma，CAMK2G）多态性与精神分裂症的关系。方法采用TaqMan探针基因分型技术在中国汉族人群中对1041例精神分裂症患者和953例正常对照者进行miR-219基因rs107822和CAMK2G基因rs2306327的分型。利用二级结构预测软件CentroidFold预测rsl07822对miR．219二级结构影响。结果miR-219基因rs107822多态的等位基因分布在患者组与正常对照组之间差异有统计学意义（X2=7．33，P=0．007）；在非条件Logistic回归分析中，rs107822基因型分布最符合加性遗传模式（P=0．0072），携带C等位基因可降低精神分裂症的发生风险（OR=O．84，95％CI=O．74～0．95）；软件预测发现rs107822T等位基因突变成C等位基因后，miR-219基因的初级转录产物的二级结构发生了改变。CAMK2G基因多态性rs2306327的等位基因和基因型分布在病例组与对照组间的差异均无统计学意义（均P〉0．05）。结论在中国汉族人群中，miR-219基因rs107822多态可能与精神分裂症有关。
基金Supported by the National High-Tech Research and Development (863) Program of China (No. 2003AA603430) and the National Natural Science Foundation of China (No. 30371092)
文摘Many of the effects of Ca^2＋ signaling are mediated through the Ca^2＋/calmodulin complex and its acceptors, the Ca^2＋/calmodulin-dependent protein kinases, including PSKHI. Studies of the proteins involved in the calcium metabolism in oysters will help elucidate the pearl formation mechanism. This paper describes a full-length PSKH1 cDNA isolated from pearl oyster Pinctada fucata. Oyster PSKH1 shares 65% homology with human PSKH1 and 48% similarity with rat CaM kinase I in the amino acid sequence, and contains a calmodulin-binding domain. The results of semi-quantitative reverse transcription-polymerase chain reaction and in situ hybridization revealed that oyster PSKH1 mRNA is highly expressed in the outer epithelial cells of the mantle pallial and in the gill epithelial cells. These studies provide important information describing the complex Ca^2＋ signaling mechanism in oyster calcium metabolism.
