Campylobacter species belong to the most important foodborne bacteria which cause gastroenteritis in humans in both developed and developing countries. Applied hygienic measures result only partially efficient, as dem...Campylobacter species belong to the most important foodborne bacteria which cause gastroenteritis in humans in both developed and developing countries. Applied hygienic measures result only partially efficient, as demonstrated by the high number of reported cases in the human population. Alternative strategies to prevent the disease though a direct action at primary production level have been explored. Preliminary data showed that cattle and pigs fed with bamboo and olive by-products, respectively, were free from Campylobacter, suggesting potential sustainable prevention strategy. In addition, biochemical analysis showed high levels of unsaturated fatty acids in red meat and meat products, adequate for the application of health claims in line with EU food law.展开更多
Campylobacter species are a major cause of foodborne bacterial infections in both developed and developing countries worldwide.Campylobacter jejuni is responsible for the majority of infectio ns.This study was con duc...Campylobacter species are a major cause of foodborne bacterial infections in both developed and developing countries worldwide.Campylobacter jejuni is responsible for the majority of infectio ns.This study was con ducted to identify virule nceassociated genes in Campylobacter species isolated from livestock production systems in South Africa.A total of 250 fecal samples consisting of cattle(n=50),chickens(n=50),goats(n=50),sheep(n=50)and pigs(n=50)were randomly collected from livestock in Eastern Cape and KwaZulu-Natal provinces of South Africa between April and October 2018.The samples were an a lyzed for the presence of virule nee genes in Campylobacter species using molecular PCR-based methods.It was found that 77 and 23%of Campylobacter jejuni and Campylobacter coli respectively were isolated from all the livestock samples.There were positive significant(P<0.05)correlations amongst all the virulenee genes that were investigated.Chisquare and Fisher's exact tests were implemented to test for the effect of livestock species on the presenee or absenee of virule nee gen es.The study dem on strated that most of livestock species can pote ntially cause zoonotic infecti on s and food pois oning due to the high prevale nee of Campylobacter.The high prevale nee of virule nee genes highlights the sign ifica nee of Campylobacter\r\livestock production systems in South Africa.This requires the implementation of one-health approaches to reduce the impact of foodborne and zoonotic diseases for the welfare of human and animal health.展开更多
The house fly, Musca domestica, has been implicated as a vector of Campy- lobacter spp., a major cause of human disease. Little is known whether house flies serve as biological amplifying hosts or mechanical vectors f...The house fly, Musca domestica, has been implicated as a vector of Campy- lobacter spp., a major cause of human disease. Little is known whether house flies serve as biological amplifying hosts or mechanical vectors for Campylobacterjejuni. We in- vestigated the period after C. jejuni had been ingested by house flies in which viable C. jejuni colonies could be isolated from whole bodies, the vomitus and the excreta of adult M. domestica and evaluated the activation of innate immune responses of house flies to ingested C. jejuni over time. C. jejuni could be cultured from infected houseflies soon after ingestion but no countable C. jejuni colonies were observed 〉 24 h postingestion. We detected viable C. jejuni in house fly vomitus and excreta up to 4 h after ingestion, but no viable bacteria were detected 〉 8 h. Suppression subtractive hybridization identi- fied pathogen-induced gene expression in the intestinal tracts of adult house flies 4-24 h after ingesting C. jejuni. We measured the expression of immune regulatory （thor, JNK, and spheroide） and effector （cecropin, diptericin, attacin, defensing, and lysozyme） genes in C. jejuni-infected and -uninfected house flies using quantitative real time PCR. Some house fly factor, or combination of factors, eliminates C. jejuni within 24 h postingestion. Because C. jejuni is not amplified within the body of the housefly, this insect likely serves as a mechanical vector rather than as a true biological, amplifying vector for C. jejuni, and adds to our understanding of insect pathogen interactions.展开更多
文摘Campylobacter species belong to the most important foodborne bacteria which cause gastroenteritis in humans in both developed and developing countries. Applied hygienic measures result only partially efficient, as demonstrated by the high number of reported cases in the human population. Alternative strategies to prevent the disease though a direct action at primary production level have been explored. Preliminary data showed that cattle and pigs fed with bamboo and olive by-products, respectively, were free from Campylobacter, suggesting potential sustainable prevention strategy. In addition, biochemical analysis showed high levels of unsaturated fatty acids in red meat and meat products, adequate for the application of health claims in line with EU food law.
基金We would like to thank the South African National Research Foundation for supporting this research through the Thuthuka Funding Instrument(TTK170411226583).We would also like to thank the College of Agriculture,Engineering and Science as well as the School of Life Sciences at University of KwaZulu-Natal(Westville Campus),South Africa for their support during the execution of this research.Authors would like to thank the anonymous reviewers for their valuable comments that significantly improved the manuscript.
文摘Campylobacter species are a major cause of foodborne bacterial infections in both developed and developing countries worldwide.Campylobacter jejuni is responsible for the majority of infectio ns.This study was con ducted to identify virule nceassociated genes in Campylobacter species isolated from livestock production systems in South Africa.A total of 250 fecal samples consisting of cattle(n=50),chickens(n=50),goats(n=50),sheep(n=50)and pigs(n=50)were randomly collected from livestock in Eastern Cape and KwaZulu-Natal provinces of South Africa between April and October 2018.The samples were an a lyzed for the presence of virule nee genes in Campylobacter species using molecular PCR-based methods.It was found that 77 and 23%of Campylobacter jejuni and Campylobacter coli respectively were isolated from all the livestock samples.There were positive significant(P<0.05)correlations amongst all the virulenee genes that were investigated.Chisquare and Fisher's exact tests were implemented to test for the effect of livestock species on the presenee or absenee of virule nee gen es.The study dem on strated that most of livestock species can pote ntially cause zoonotic infecti on s and food pois oning due to the high prevale nee of Campylobacter.The high prevale nee of virule nee genes highlights the sign ifica nee of Campylobacter\r\livestock production systems in South Africa.This requires the implementation of one-health approaches to reduce the impact of foodborne and zoonotic diseases for the welfare of human and animal health.
文摘The house fly, Musca domestica, has been implicated as a vector of Campy- lobacter spp., a major cause of human disease. Little is known whether house flies serve as biological amplifying hosts or mechanical vectors for Campylobacterjejuni. We in- vestigated the period after C. jejuni had been ingested by house flies in which viable C. jejuni colonies could be isolated from whole bodies, the vomitus and the excreta of adult M. domestica and evaluated the activation of innate immune responses of house flies to ingested C. jejuni over time. C. jejuni could be cultured from infected houseflies soon after ingestion but no countable C. jejuni colonies were observed 〉 24 h postingestion. We detected viable C. jejuni in house fly vomitus and excreta up to 4 h after ingestion, but no viable bacteria were detected 〉 8 h. Suppression subtractive hybridization identi- fied pathogen-induced gene expression in the intestinal tracts of adult house flies 4-24 h after ingesting C. jejuni. We measured the expression of immune regulatory （thor, JNK, and spheroide） and effector （cecropin, diptericin, attacin, defensing, and lysozyme） genes in C. jejuni-infected and -uninfected house flies using quantitative real time PCR. Some house fly factor, or combination of factors, eliminates C. jejuni within 24 h postingestion. Because C. jejuni is not amplified within the body of the housefly, this insect likely serves as a mechanical vector rather than as a true biological, amplifying vector for C. jejuni, and adds to our understanding of insect pathogen interactions.
