Design of a Landscape Device for Children from the Perspective of Embodied Cognition in the New Era

In recent years,embodied cognition has ushered in a new research upsurge in the academic field,and has become a hot topic in the field of cognitive psychology.In this paper,from the perspective of embodied cognition,the interaction ways of a landscape device for children were discussed to achieve a more real and harmonious interaction between children and scenes.The research data of embodied cognition used by children was analyzed,and the drawbacks and breakthrough points of current landscape devices for children were discussed.The core characteristics of children’s growth period were extracted to establish children’s interaction model and summarize the interactive design methods of landscape devices for children.Embodied cognition has become the most intuitive way for children to know and understand the environment,and plays a pivotal role in children’s growth.Based on embodied cognition principle and interactive behavior mode,the interactive design of a landscape device for children was studied,and three interactive design modes,including simple and convenient interaction mode,multi-sensory interaction mode and game natural interaction mode were summarized.On the basis of this research,relevant design practice and research were carried out to bring a new vision to the design of children’s landscape.

Emergency Care for Children and Adolescents after Sexual Violence, in Brazil’s Capital, Brasilia

Objective: Sexual violence affects women of all ages but, more commonly, children and adolescents. This study aimed to identify the extent to which services are being provided at the hospitals responsible for the emergency care of children and adolescents who have suffered sexual violence and to determine whether there is any difference in the provision of such services as a function of the victim’s age. Method: This cross-sectional study investigated differences in the care received by female children and adolescents who are victims of sexual violence in a capital city, comparing the treatment given to girls under 15 with that given to adolescents of 15 to 19 years of age. The data were extracted from the State Department of Health database between 01/01/2012 and 31/12/2016. Results: Children under 15 were much less likely to receive prophylaxis against HIV, sexually transmitted infections and hepatitis B, with the number of adolescent girls treated adequately corresponding to between twice and almost three times the number of children treated prophylactically. Only 10% of girls under 15 received emergency contraception. Conclusion: The lack of standard care established by the Ministry of Health highlights the need for urgent debates both locally and probably throughout the country, as the situation may be similar in other regions of the country.

Mapping the Research Landscape of Language Development in Autistic Children: A Preliminary Scientometric Analysis

Children with autism spectrum disorder(ASD)often encounter difficulties in language learning and utilization,a concern that has gained significant academic attention,particularly given the widespread occurrence of ASD globally.Previous reviews,however,have relied on empirical observations rather than a more rigorous selection criterion.This preliminary study seeks to systematize the scientific knowledge base regarding language development in autistic children by utilizing the analysis tool Citespace 6.2.R5.We visualized and analyzed research patterns and trends regarding autism by drawing data from the Web of Science.Through document citation and emerging trend analyses,seven key research clusters and their chronological associations are identified,along with research hotspots such as language disorder diagnosis and intervention,social communication,language acquisition,and multilingual and multicultural influences.Research findings show that there exist some issues with the current research,including small sample sizes,the need for further investigation into receptive language development,and a lack of cross-cultural comparative studies.Meanwhile,the scope and depth of interdisciplinary research on language development in autistic children also need to be further enhanced.The research contributes to the extant literature by providing valuable references for autism researchers and practitioners.

Pupillary capture following sutureless scleral-fixated intraocular lens in children with Marfan syndrome

AIM:To compare the clinical outcomes between two approaches for sutureless scleral-fixated intraocular lens(SFIOL)in children with Marfan syndrome(MFS).METHODS:The study included 15 children(26 eyes)with lens subluxation due to MFS.These children underwent lensectomy,anterior vitrectomy,and sutureless SFIOL.According to the position of placement of intraocular lens(IOL)haptics,two study groups were reviewed for best corrected visual acuity(BCVA)and postoperative complications:group A,14 eyes with haptics fixated at 2.0 mm from the limbus;group B,12 eyes with the haptics fixated at 2.5 mm from the limbus.RESULTS:The mean axial length for all patients was 25.66±2.35 mm.Postoperative BCVA in logMAR were significant improved in both groups(0.77±0.32 to 0.17±0.12 in group A,0.66±0.25 to 0.24±0.12 in group B,both P<0.001)while no significant difference between two groups(P>0.05).Pupillary capture was main postoperative complication,occurring between 3d and 18mo.It occurred in 7 eyes in group A and one eye in group B(P=0.02).CONCLUSION:Sutureless SFIOL is an effective treatment approach for lens subluxation in children with MFS.Pupillary capture is the main postoperative complication.Fixated IOL haptics at 2.5 mm from the limbus can reduce the occurrence of pupillary capture.

Recreation of Agricultural Landscape for Children with Nature-deficit Disorder

Promoting human health is a basic value of landscape architecture. It is theoretically feasible to rebuild the link between children with nature-deficit disorder and nature via artificially-made natural environment. This paper explored the relationship between children's health and nature from the perspective of landscape architecture theories and practices. First, impact of nature-deficit disorder on children's health; second, basic appeals of children with nature-deficit disorder were sorted out; finally, specialized gardens for children with nature-deficit disorder under the instruction of the above theories were designed.

Safety and utility of capsule endoscopy for infants and young children

AIM:To assess the safety and utility of capsule endoscopy(CE)for children who are unable to swallow the capsule endoscope.METHODS:The medical records of all of the children who underwent CE between 2010 and 2012 were retrospectively reviewed.The patients were divided into 2groups:group A included patients who were unable to swallow the capsule endoscope,and group B included patients who were able to swallow it.For the patients who were unable to swallow the capsule endoscope,it was placed in the duodenum endoscopically.The small bowel transit time,endoscopic diagnosis and complications of the 2 groups were compared.RESULTS:During the study period,28 CE procedures were performed in 26 patients.Group A included 11patients with a median age of 2 years(range 10 mo-9years),and group B included 15 patients with a median age of 12 years(range 8 years-16 years).The lightest child in the study weighed 7.9 kg.The detection rates did not differ between the 2 groups.The median small bowel transit time was 401 min(range 264-734 min)in group A and 227 min(range 56-512 min)in group B(P=0.0078).No serious complications,including capsule retention,occurred.No significant mucosal trauma occurred in the pharynx,esophagus,stomach or duodenum when the capsule was introduced using an endoscope.CONCLUSION:CE is a safe and useful procedure for infants and young children who are unable to swallow the capsule endoscope.

Prevalence of Iron Deficiency and Iron Deficiency Anemia in Infants and Children and Treatment with Microencapsulated Iron II Fumarate and Supplied Ascorbic Acid as “Sprinkles”

Background: Iron deficiency anemia (IDA) in children and infants is a common nutritional problem all over the world. Infants and young children have a high risk for developing iron deficiency (ID) because they have high demand for iron during the period of rapid growth. This is aggravated by the insufficiency of iron in their diet. Iron supplementation programs using pediatric tablets or drops have not been successful in the control of anemia amongst infants and children in some countries. “Sprinkles” is an innovative multi-micronutrient home fortification strategy to control iron deficiency and anemia to be more useful. Objective: The objective was to estimate the prevalence ID and IDA in infants and children in Ibb City, Yemen Republic;evaluate the use of a new form of iron and determine the hematologic response to different doses and forms of iron in Sprinkles and iron drops. Design: Using a prospective, randomized, controlled design, we studied 337 randomly children aged 24 - 48 months and infants aged 6 - 24 months in Ibb City (hemoglobin: 70 - 99 g/L). One group received a daily sachet of microencapsulated ferrous fumarate (80 mg elemental Fe) in powder form plus ascorbic acid to be sprinkled onto any complementary food eaten (sprinkles group);a control group received ferrous sulfate drops 3 times/d for 2 months (total dose: 40 mg elemental Fe). Hemoglobin and serum ferritin concentrations were measured at baseline and at the end of treatment. Results: Successful treatment of anemia (hemoglobin > 100 g/L) occurred in 58% of the sprinkles group and in 56% of the drops group, with minimal side effects in both groups. Geometric mean ferritin concentrations increased significantly in each group from baseline to the end of treatment (P Conclusion: Use of ferrous sulfate drops or a single daily dose of microencapsulated ferrous fumarate sprinkles plus ascorbicacid resulted in a similar rate of successful treatment of anemia without side effects. To our knowledge, this is the first demonstration of the use of microencapsulated iron sprinkles to treat anemia in this area. Improved ease of use may favor the use of sprinkles to deliver iron.

Comparison of 25-gauge sutureless vitrectomy and 20-gauge vitrectomy in the treatment of posterior capsule opacification in pseudophakic children

AIMTo compare the effectiveness and safety of pars plana capsulotomy and vitrectomy using 25-gauge tansconjunctival sutureless vitrectomy system and 20-gauge vitrectomy system for posterior capsule opacification (PCO) in pseudophakic children.METHODSRetrospectively study. Pars plana capsulotomy and vitrectomy using 25-gauge sutureless vitrectomy system was performed for PCO in the study group (32 eyes). Patients in the control group (34 eyes) underwent capsulotomy and vitrectomy using standard 20-gauge vitrectomy system, providing a comparison between 2 groups with regard to preoperative and postoperative best corrected visual acuity (BCVA), intraocular pressure (IOP), and intraoperative and postoperative complications. The two groups were performed consequentially. The patients ages ranged from 2 to 13y (means: 6.61&#x000b1;2.73y). Surgical technique, intraoperative and postoperative complications, visual acuity, IOP, and recurrent PCO were recorded.RESULTSThe surgical procedure was performed uneventfully in all patients. Visual acuity improved significantly in both groups. BCVA improved in 22 eyes (81.5%) in the study group and in 28 eyes (87.5%) in the control group. There was no statistical difference of visual acuity that were attainable in two groups (H=0.115, P=0.909). Mean postoperative IOP showed no significant difference between the groups at 1wk. All sort of PCO were accomplished by 20-gauge system, while 25-gauge system was effective for pearls style and 2 grade of fibrous PCO, and was insufficient to grade 3 of PCO. In the study group two cases were not accomplished by 25-gauge system while 20-gauge system conquered them. Compared with the control group, mean operative time for opening and closing the sclerotomy in the study group was considerably reduced. The mean follow-up was 38.2mo (range: 8-79mo). During the follow-up period, no incision leakage, corneal edema, vitreous loss, IOL damage, retinal detachment, recurrent PCO, or other complications were noted.CONCLUSIONPars plana capsulotomy and vitrectomy using 25-gauge transconjunctival sutureless vitrectomy appeared to be a safe and effective approach for PCO in pseudophakic children. Combined sutureless surgery needed shorter setup time for sclerotomy and caused less surgical trauma than combined surgery with 20-gauge vitrectomy. Therefore, this type of procedure would be a good option for selected cases with PCO in pseudophakic children.

Comparison of the use of wireless capsule endoscopy with magnetic resonance enterography in children with inflammatory bowel disease

BACKGROUND Magnetic resonance enterography (MRE) and wireless capsule endoscopy (WCE) are equally accepted modalities for noninvasive screening of small bowel involvement (SBI) in children with Crohn’s disease (CD) and indeterminate colitis (IC) albeit there is a paucity of data comparing the two and thereby guiding the clinician in selecting the ideal diagnostic approach. Therefore, the goal of this study is to provide additional evidence for capsule endoscopy role in the evaluation of established Crohn’s disease exacerbation compared to MRE in relation to Pediatric Crohn's Disease Activity Index (PCDAI), and histological indices. AIM To prospectively compare the findings of MRE and WCE and their agreement with PCDAI or histology in children with CD or IC. METHODS Consecutive patients diagnosed with CD and IC were screened for inclusion. After informed consent, patient’s demographic and clinical data was abstracted. The current pediatric disease activity index (PCDAI) and endoscopic findings were included. Patients underwent MRE and WCE including preprocedural patency capsule within a maximum of 7 d of each other. Pathological presence of active small bowel disease in ileal and duodenal biopsies were collected if the endoscopy was performed within 2 mo of the WCE study. Patients who failed to pass the PC were excluded from the study. WCE was read by two different experienced gastroenterologists (Attard TM and Colombo JM) blinded to each other's findings and to the findings on MRE (Mardis NJ). Agreement between WCE reviewers, WCE and MRE findings and concordance between positive PCDAI and SBI based on MRE compared with WCE was computed. RESULTS Forty-five patients were included in the study, 18 withdrew and 27 (20 males and 20 CD), mean age (standard deviation) 13.46 (2.4) years, completed the study protocol. There were no instances of capsule retention. Concordance between gastroenterologist reviewers was excellent for the diagnosis of small intestinal CD with good correlation between the two Lewis scores (r=0.875, P<0.001). Concordance between WCE and MRE was poor (69%). In CD patients, when both MRE and WCE were compared using PCDAI>10 as the standard reference reflecting active small intestinal CD, the sensitivity of MRE and WCE were 100% and 83% respectively and the specificity of MRE and WCE were 57.14% and 78.6%, respectively. If the histology in ileum or/and duodenum was used as the reference for active small bowel involvement, WCE had a higher specificity as compared to MRE (83.3% vs 50%). In patients with Crohn’s disease, those with a positive PCDAI (>10) were more likely to have a positive WCE as compared to those with a negative PCDAI (83% vs 21%;P=0.018). CONCLUSION We suggest that MRE and WCE have a complementary role in the assessment of SBI in CD. WCE detected SBI with a much higher specificity while MRE had a higher sensitivity.

Design Elements of Urban Children' s Recreational Landscape Space Based on Genius Loci

Urban children's recreational landscape space is an organic component of urban children's recreational space,genius loci is both a result and a motive in its design.This paper interpreted the connotations of genius loci of urban children's recreational space,the correlation between both,and design elements of urban children's recreational landscape space,in order to provide theoretical support for the design of urban children's recreational landscape space.

Childhood Obesity in Primary School Children of Middle and Upper-Middle Income Group in the Capital City of Bangladesh

Background: No published information is available on the status of childhood obesity in Bangladeshi primary school children of urban middle and upper-middle income group. The aim of the study was to explore the proportion of childhood obesity in the primary school children of middle and upper-middle income group in capital city and to gather information on their dietary pattern and physical activity. Methods: One hundred and fifty children (age 9 ± 1 years, height (ht) 118 ± 20 cm, weight (wt) 36 ± 8 kg, wt/ht% 126 ± 24, mean ± SD) were selected purposively from the primary schools of Dhaka city. On the basis of predefined scoring, subjects were classified as underweight, normal, overweight and obese if their weight-for-height values were 120% of median values for Bangladeshi children respectively. Dietary intake and energy expenditure of the study subjects were calculated by standard techniques. Results: The proportion of childhood overweight and obesity was 52% and 20%. The proportion of overweight and obesity was higher (78%) among girls compared to boys (54%). Similarly, height, weight and other anthropometrics indices were significantly (p < 0.05) higher in girls compared to boys. The daily intake of carbohydrate and protein was significantly (p < 0.05) higher among obese group. Total energy balance was significantly correlated with weight-for-height% (r = 0.541, p = 0.0001). Conclusions: A higher proportion of overweight and obesity had been found in girls’ children than their counter parts in this study. Positive energy balance with higher energy intake from carbohydrate and fast/processed food culture habit were found among primary school children of middle and upper-middle income group in the capital city of Bangladesh.

Psychological Capital Status of Left-Behind Rural Children in China and Its Relationship with Mental Health

Background:The mental health of left-behind rural children of China has become a prominent social problem.At the same time,psychological capital has become a positive psychological resource to promote personal growth.However,the relationship between psychological capital and mental health of left-behind rural children has not been explored.Method:A total of 332 left-behind rural children were investigated using a questionnaire of psychological capital and mental health scale.The psychological capital status of left-behind rural children and its impact on mental health were analyzed.Results:(1)Left-behind rural children were found to have a psychological capital score of was 3.16±0.56;Gender,age,grade,and school style had no significant effect on psychological capital.(2)Left-behind rural children obtained a mental health score of 2.21±0.5 G and gender,age,grade,and school style had no significant effect on mental health.(3)A significant negative correlation is found between psychological capital and mental health scores of left-behind rural children.Regression analysis shows that psychological capital is negatively correlated with mental health scores.Conclusion:The psychological capital of left-behind rural children can positively predict their mental health,and thus developing the former can promote the latter.

Tangents on TV： The Tangential Relationship of Narrative and Educational Content in Children＇s Television Through the Capacity Model

Previous studies have shown that children learn from educational television; however, these studies have not explored how closely the educational content of the program is integrated to the narrative. The following study uses a systematic content analysis of 100 top-rated U.S. children＇s television programs to explore the tangential relationship （i.e., distance） of narrative and educational content through the lens of the capacity model--which is built upon three basic components： narrative content, educational content, and the distance between them. Programs were analyzed for distance by examining lesson clarity and integration, show type, target audience age, and station type. One-way ANOVAs indicates significant differences between the means of these variables. Programs targeting younger children presented educational content that was closely integrated into the plotline of the program and helped further the story. However, as the target audience aged, the educational content became more tangential to the narrative content and was less tied to the plotline of the program.

Intellectual Capacity of Children Exposed to Environmental Pollution in the Extreme South of Brazil

Objective： The study evaluated the intellectual capacity of children who live in areas that were E （exposed） or NE （not exposed） to environmental pollution. Method： 246 students between the ages of 8 and 11 years and 8 months were evaluated. Data were collected between May and June 2010 using a semi-structured questionnaire to examine factors that were related to compromised intellectual capacity. Intellectual capacity was evaluated with Raven＇s Colored Progressive Matrices Scale. The intellectual capacity of children who live in areas that were exposed or not exposed was compared using chi-squared tests and raw and adjusted analyses were conducted using Poisson regression. Results： The general occurrence of intellectual impairment was 28.9%, with 22.0% in the non-exposed area and 36.1% in the exposed area （p = 0.01）. The outcome was associated with the following： a lack of companionship （RP = 2.77; p 〈 0.00）, maternal education of less than five years （RP = 2.13; p 〈 0.00）, the mother not being Caucasian （RP = 1.57;p 〈 0.00）, residing in an exposed area （RP = 1.88;p 〈 0.00）, and nutritional risk/malnutrition （RP = 2.83; p 〈 0.00）. Conclusions： The results showed a high occurrence of intellectual impairment and a significant difference between the environmental areas. Additional research is needed to improve the understanding of several results. The town＇s priorities should include efforts related to environmental health.

The impact of social capital on children's (6–12) use of ICT in urban communities: A field survey from Zhengzhou City, Henan Province

Purpose： This paper focuses on the impact of social capital on urban children＇s use behavior of information communication technology （ICT）.Design/methodology/approach： Using the field survey and in-depth interviews, we interviewed 40 children aged 6 to 12 and their parents from a staff residential quarter of the Zhengzhou University--＂Shengheyuan＂ community （SHY）, and a commercial residential quarter--＂Wanfenghuicheng＂ community （WFHC） in the high-tech zone of Zhengzhou City, Henan Province. We used the social capital theory to analyze the interviewees＇ record.Findings： In urban communities, social capital is the most important factor for children （aged 6 to 12） in their ICT use. Our findings indicate that children in families with higher levels of social capital, such as internal resources, family income, parent educational backgrounds and parents＇ social network, have more-highly developed ICT skills. Personal motivation and obstacles, such as lack of access to computers on a regular basis, also have an impact on children＇s ICT use. External social capital, including schools, libraries, and public service institutes, have little impact on children＇s ICT use, if not combined with internal social capital factors.Research limitations： Our research samples were collected from two communities within the same city, which may influence the generalization of this research result.Originality/value： To explore the social capital＇s influence on children＇s ICT use, we used field observation for ICT use of children aged 6 to 12 in urban communities in China, and studied the children＇s ICT behavior from the perspective of internal and external social capital.

Cap polyposis: A rare cause of rectal bleeding in children

AIM: To evaluate the clinicopathological features and treatment outcomes of cap polyposis in the pediatric population. METHODS: All pediatric patients with histologically proven diagnosis of cap polyposis were identified from our endoscopy and histology database over a 12 year period from 2000-2012 at our tertiary pediatric center, KK Women's and Children's Hospital in Singapore. The case records of these patients were retrospectively reviewed. The demographics, clinical course, laboratory results, endoscopic and histopathological features, treatments, and outcomes were analyzed. The study protocol was approved by the hospital institutional review board. The histological slides were reviewed by a pediatric histopathologist to confirm the diagnosis of cap polyposis. RESULTS: Eleven patients were diagnosed with cap polyposis. The median patient age was 13 years (range 5-17 years); the sample included 7 males and 4 females. All of the patients presented with bloody stools. Seven patients (63%) had constipation, while 4 patients (36%) had diarrhea. All of the patients underwent colonoscopy and polypectomies (excluding 1 patient who refused polypectomy). The macroscopic findings were of polypoid lesions covered by fibrinopurulent exudates with normal intervening mucosa. The rectum was the most common involvement site (n = 9, 82%), followed by the rectosigmoid colon (n = 3, 18%). Five (45%) patients had fewer than 5 polyps, and 6 patients (65%) had multiple polyps. Histological examination of these polyps showed surface ulcerations with a cap of fibrin inflammatory exudate. Four (80%) patients with fewer than 5 polyps had complete resolution of symptoms following the polypectomy. One patient who did not consent to the polypectomy had resolution of symptoms after being treated with sulphasalazine. All 6 patients with multiple polyps experienced recurrence of bloody stools on follow-up (mean = 28 mo). CONCLUSION: Cap polyposis is a rare and underrecognised cause of rectal bleeding in children. Our study has characterized the disease phenotype and treatment outcomes in a pediatric cohort.

Inclusive Architecture:Landscape Codesign in Children’s Playgrounds

Children’s playgrounds are open spaces,the basis for children’s recreation,important for the inclusion and mobility of visually impaired children in the social environment,through inclusive urban facilities that stimulate new experiences for their cognitive development.In this context,the use of co-design with visually impaired people,in the design processes of children’s playgrounds,assumes an importance for an inclusive project based on their experiences.Thus,it aimed to promote a project together,to provide more comfort and safety to users.It presents as main results as better colors,materials and types of toys for children with visual impairment to be competent in a playground including from the application of methods,tools and resources in the co-design process.

猪圆环病毒3型Cap蛋白单克隆抗体的制备及阻断ELISA检测方法的建立

旨在建立检测猪圆环病毒3型(PCV3)抗体的阻断ELISA方法,本研究利用原核表达的PCV3Cap重组蛋白免疫BALB/c小鼠制备获得了一株分泌阻断效果良好抗体的杂交瘤细胞株2E6。以重组Cap蛋白作为包被抗原,以辣根过氧化物酶(HRP)标记的2E6单克隆抗体作为检测抗体,经条件优化后建立了一种检测PCV3抗体的阻断ELISA方法。用建立的阻断ELISA方法检测50份临床阴性血清,计算阻断率(PI)的临界值,以此来确定该方法的判定标准:当PI≤28.30%时,判定结果为阴性;当PI≥35.05%时,判定结果为阳性;当28.30%<PI<35.05%时,判定为可疑,重复一次试验后如果结果仍为可疑,则判定为阳性。特异性试验表明该方法与猪圆环病毒2型(PCV2)、猪伪狂犬病病毒(PRV)、猪繁殖与呼吸综合征病毒(PRRSV)以及猪瘟病毒(CSFV)的阳性血清均无交叉反应;敏感性试验表明其检测效价可达到1:128;重复性试验表明批内与批间的变异系数均小于10%;符合性检验表明该方法与PCV检测金标准免疫过氧化物酶单层试验(IPMA)比对的Kappa值达0.9,具有高度的一致性。综上所述,本研究建立的阻断ELISA方法具有良好的特异性与较高的符合率,可用于后期进行PCV3抗体的检测,为PCV3的流行病学调查与临床诊断提供技术支持。

猪圆环病毒2型Cap蛋白的原核表达及间接ELISA抗体检测方法的初步建立

Cap蛋白作为猪圆环病毒2型(PCV2)的主要结构蛋白,构成病毒的核衣壳,是PCV2的主要免疫保护性抗原,在PCV2血清学诊断中具有重要意义。本研究根据PCV2的ORF2基因序列设计特异性引物,通过PCR方法扩增得到去核定位信号肽的ORF2基因,并通过同源重组将其克隆至原核表达载体pCold-Ⅰ中,经测序鉴定成功获得重组质粒pCold-Ⅰ-Cap。将重组质粒转化至感受态细胞BL21中进行表达,通过考马斯亮蓝染色以及免疫印迹试验检测Cap蛋白的表达,同时将纯化后的重组蛋白通过优化反应条件建立检测PCV2血清抗体的间接ELISA方法。结果表明:重组去核定位信号肽Cap蛋白可溶性表达且可以与PCV2阳性血清特异性结合,通过探索不同蛋白包被浓度以及不同一抗孵育浓度初步建立了间接ELISA检测方法,进而为PCV2抗体的有效监测奠定基础。

昆虫杆状病毒表达的猪圆环病毒2d型Cap蛋白-VLP对仔猪的免疫保护效果

猪圆环病毒2d基因型(Porcine circovirus type 2d,PCV2d)为猪场当前流行的PCV2优势基因型,为了研究针对PCV2d基因型的病毒样颗粒(Virus-like particle,VLP)疫苗,提高猪圆环病毒病的防控效果。本研究利用杆状病毒表达系统表达了能够自组装为VLP的PCV2d-Cap蛋白,然后将9头21日龄健康仔猪随机分为VLP组、攻毒对照组和空白对照组(n=3)。VLP组的每头仔猪经颈部肌肉注射400μg Cap蛋白-佐剂复合物(PCV2d-VLP),攻毒组注射等体积的PBS与佐剂混合物,空白组注射等体积的PBS。共接种2次,每次间隔14 d。于第2次免疫后21 d,VLP组和攻毒对照组的仔猪通过鼻腔感染PCV2 HBDX-2018株(106TCID50/头),评价PCV2d-VLP诱导的免疫效果。结果表明PCV2d-VLP能够诱导仔猪产生高水平的特异性IgG抗体和中和抗体,刺激IFN-γ水平升高,引起外周血淋巴细胞增殖能力增强,降低病毒经鼻腔和直肠的排毒率及病毒血症阳性率,减轻腹股沟淋巴结和脾脏的病理损伤,降低组织中的病毒载量。上述研究表明,应用杆状病毒表达的PCV2d-Cap蛋白能自组装为VLP,并能诱导仔猪产生良好的免疫保护效应,具有进一步开发为PCV2d-VLP疫苗的潜力。