在莱州湾凹陷垦利10-4油田开展油气成藏条件及成藏模式研究。以地球化学测试、生物标志化合物、镜下薄片观察及钻井、录井等资料分析为基础,研究垦利10-4油田油气来源、储集性能、储盖组合、圈闭特征、输导运聚等成藏条件,结合烃类包裹...在莱州湾凹陷垦利10-4油田开展油气成藏条件及成藏模式研究。以地球化学测试、生物标志化合物、镜下薄片观察及钻井、录井等资料分析为基础,研究垦利10-4油田油气来源、储集性能、储盖组合、圈闭特征、输导运聚等成藏条件,结合烃类包裹体测定和盆地模拟方法厘定油气成藏期并建立油气成藏模式。垦利10-4油田主力产层沙三段由邻近北洼的沙三段和沙四段烃源岩共同供烃,具有近源捕集成藏的条件;储层主要为高孔高渗型辫状河三角洲前缘沉积砂体,沙三段砂岩与上覆沙一、沙二段泥岩沉积段构成了多套有利的储盖组合,且与圈闭形成期及断层活动时间匹配良好。垦利10-4油田为晚期成藏,主成藏期约2.6 Ma B.P.至今,辫状河三角洲前缘砂体与断裂、不整合面在空间上的叠置为油气输导及聚集提供了条件。成藏模式具有“近源捕集,短距离运移,晚期快速充注,深层原位成藏”的特点。展开更多
Background The capsule associated protein 10 gene （caplO） is indispensible for the formation of the polysaccharide capsule, and is important in maintaining virulence of the Cryptococcus （C.） neoformans. In this st...Background The capsule associated protein 10 gene （caplO） is indispensible for the formation of the polysaccharide capsule, and is important in maintaining virulence of the Cryptococcus （C.） neoformans. In this study, we aimed to construct an short hairpin RNA （shRNA） expression vector targeting C. neoformans caplO gene expression and confirm its biologic relevance. Methods A pair of oligonucleotides targeting the cap10 cDNA sequence was designed and synthesized. It was cloned into the plasmid psilencer4.1-CMV neo to construct an eukaryotic shRNA expression vector. The vector was transfected into C. neoformans cells using the LiAc method. The expression of cap10 was assessed by real-time fluorescence quantitative PCR. Groups of C. neoformans cells were incubated with murine macrophage-like J774A.1 cells, and the phagocytic indexes and ratios were determined by the microscopic observation method. Results The expression of cap10 in C. neoformans cells transfected with ps4.1 nee-cap10 （（175 535.00±47 004.00） copies/μl） was lower than that of cells transfected with the empty vector （（512 698.89±32 318.02） copies/μl） and mock transfected cells （（562 931.66±65 928.41） copies/μl）. The average phagocytic ratio and phagocytic index of J774A.1 cells following incubation with C. neoformans were higher for cells transfected with ps4.1 neo-capl0 （0.21±0.02, （19.06±1.66）%） than for the control experimental group （0.08±0.02, （6.57±1.23）%） and the blank experimental group （（0.07±0.01）, （5.89±1.07）%） （P 〈0.05）. Conclusions The cap10 shRNA vector was successfully prepared and transfected into C. neoformans cells. The effect of RNA interference on the expression of the C. neoformans caplO gene is effective, and it can induce phagocytosis of C. neoformans.展开更多
文摘在莱州湾凹陷垦利10-4油田开展油气成藏条件及成藏模式研究。以地球化学测试、生物标志化合物、镜下薄片观察及钻井、录井等资料分析为基础,研究垦利10-4油田油气来源、储集性能、储盖组合、圈闭特征、输导运聚等成藏条件,结合烃类包裹体测定和盆地模拟方法厘定油气成藏期并建立油气成藏模式。垦利10-4油田主力产层沙三段由邻近北洼的沙三段和沙四段烃源岩共同供烃,具有近源捕集成藏的条件;储层主要为高孔高渗型辫状河三角洲前缘沉积砂体,沙三段砂岩与上覆沙一、沙二段泥岩沉积段构成了多套有利的储盖组合,且与圈闭形成期及断层活动时间匹配良好。垦利10-4油田为晚期成藏,主成藏期约2.6 Ma B.P.至今,辫状河三角洲前缘砂体与断裂、不整合面在空间上的叠置为油气输导及聚集提供了条件。成藏模式具有“近源捕集,短距离运移,晚期快速充注,深层原位成藏”的特点。
文摘目的:探讨术中神经监测(intraoperative neuromonitoring,IONM)对分化型甲状腺癌(differentiated thyroid carcinoma,DTC)患者主观语音结局的影响。方法:回顾性分析从2018年9月—2019年3月在中山大学第一附属医院接受甲状腺手术的DTC患者,分为IONM组和非IONM组,采用倾向评分匹配法(propensity score matching,PSM),比较两组患者的临床基线特征和术后主观语音结果。结果:在预防性中央区淋巴结清扫亚组中,两组患者术后1个月、6个月的声音障碍指数⁃10(voice handi⁃cap index⁃10,VHI⁃10)总分及甲状腺癌特异性生活质量(thyroid cancer specific quality of life,THYCA⁃QoL)问卷的声音领域均无统计学差异(P>0.05)。在靠近或侵犯后包膜亚组中,IONM组术后1个月、6个月的THYCA⁃QoL问卷中的声音领域评分中位数(四分位数)均为0.00(0.00,0.00),低于非IONM组的0.00(0.00,33.33),差异有统计学意义(P=0.034)。均值均为5.73,明显低于非IONM组的15.66(P=0.029)。VHI⁃10问卷两组差异无统计学意义。结论:IONM对DTC患者及预防性中央区淋巴结清扫术亚组患者的主观发声障碍没有显著影响,但可明显改善靠近后包膜高危组患者术后主观声音异常。
文摘Background The capsule associated protein 10 gene （caplO） is indispensible for the formation of the polysaccharide capsule, and is important in maintaining virulence of the Cryptococcus （C.） neoformans. In this study, we aimed to construct an short hairpin RNA （shRNA） expression vector targeting C. neoformans caplO gene expression and confirm its biologic relevance. Methods A pair of oligonucleotides targeting the cap10 cDNA sequence was designed and synthesized. It was cloned into the plasmid psilencer4.1-CMV neo to construct an eukaryotic shRNA expression vector. The vector was transfected into C. neoformans cells using the LiAc method. The expression of cap10 was assessed by real-time fluorescence quantitative PCR. Groups of C. neoformans cells were incubated with murine macrophage-like J774A.1 cells, and the phagocytic indexes and ratios were determined by the microscopic observation method. Results The expression of cap10 in C. neoformans cells transfected with ps4.1 nee-cap10 （（175 535.00±47 004.00） copies/μl） was lower than that of cells transfected with the empty vector （（512 698.89±32 318.02） copies/μl） and mock transfected cells （（562 931.66±65 928.41） copies/μl）. The average phagocytic ratio and phagocytic index of J774A.1 cells following incubation with C. neoformans were higher for cells transfected with ps4.1 neo-capl0 （0.21±0.02, （19.06±1.66）%） than for the control experimental group （0.08±0.02, （6.57±1.23）%） and the blank experimental group （（0.07±0.01）, （5.89±1.07）%） （P 〈0.05）. Conclusions The cap10 shRNA vector was successfully prepared and transfected into C. neoformans cells. The effect of RNA interference on the expression of the C. neoformans caplO gene is effective, and it can induce phagocytosis of C. neoformans.
