Two Novel Saponins from Lysimachia capillipes

Two new saponins named capilliposide C 1 and capilliposide D 2 were isolated from the whole plants of Lysimachia capillipes, their structures were deternuned by 1D and 2D NMR, ESIMS techniques, and chemical methods.Capilliposide C showed significant cytotoxic activity against human A2780 cells.

PHYTOECDYSONES FROM THE ROOTS OF TINOSPORA CAPILLIPES

In addition to 2-deoxycrustecdysone(1),we isolated and identi- fied two new natural moulting hormones-2-deoxy-3-epicrustecdysone(2)and 2-deoxycrustecdysone-3-O-β-D-glucopyranoside(3)from the roots of Tino- spora capillipes,it is the first time to get ecdysteroids from this genus.

细梗香草及近缘种内生真菌的多样性

研究细梗香草(Lysimachia capillipes Hemsl.)及近缘种内生真菌多样性,为开发新微生物药物资源奠定基础。采用4种不同培养基分离不同地点样品内生真菌菌种,提取菌丝DNA,扩增ITS序列,测序后GenBank中Blast对比得到同源相似度较高的菌种。获得分离内生真菌共258株;马铃薯葡萄糖琼脂培养基(PDA)和改良马丁氏培养基(改良MRS)分离内生真菌数量较多,分别占总数的32.56%和28.68%,菌种生长速率较快,而水琼脂(WA)培养基分离菌种数最少,占总数的17.05%,菌落生长速率较慢。相似度较高的菌种有27种,占总菌种数的10.47%。PDA和改良MRS是分离培养细梗香草内生菌较好的培养基,分离得到的菌种重复性较高,4种培养基分离的菌种差异较小。

Two New Saponins from Lysimachia capillipes Hemsl.

To investigate the saponins from whole plants of Lysimachia capilUpes Hemsl., two new saponins, named capilliposide E （1） and capilliposide F （2）, were isolated. The structures of the new saponins were elucidated as 3β, 22α-dihydroxy-16α-acetat-28→ 13-lactone-oleanane-3-O- [β-D-glucopyranosyl- （1 →2）-α-L-arabinpyranoyl]-22-O-β-D-glucopyranoside （1） and 3β, 22α-dihydroxy- 16α-acetat-28→ 13-lactone-oleanane-3-O-{ [β-D-glucopyranosyl-（1→2）-β-D-glucopyranosyl-（1→4）]-α-L-arabinpyranoyl}-22-O-β- D-glucopyranoside （2）. The structures of these compounds were determined by 1D- and 2D-NMR, MS techniques, and chemical methods.

Capilliposide from Lysimachia capillipes promotes terminal differentiations and reverses paclitaxel resistance in A2780T cells of human ovarian cancer by regulating Fos/Jun pathway

Objective:To investigate the potential effect of Lysimachia capillipes capilliposide(LCC)on the chemo sensitivity and the stemness of human ovarian cancer cells.Methods:Cell Counting Kit-8(CCK8)was used to measure the IC50 values.The apoptosis of cells was measured through flow cytometry.Evaluation of the stemness and differentiation markers was performed by the immunoblotting and the immunostaining assays.RNA-seq was performed through the Illumina HiSeq PE150 platform and differentially expressed genes(DEGs)were screened out through the bioinformation analysis.Overexpression or knockdown of Fos gene was achieved by shRNA transfection.Results:Pre-exposure of A2780T cells with 10μg/mL LCC sensitized them to paclitaxel,of which the IC50 value reduced from 8.644μmol/L(95%CI:7.315–10.082μmol/L)to 2.5μmol/L(95%CI:2.233–2.7882μmol/L).Exposure with LCC enhanced the paclitaxel-induced apoptosis and inhibited the colony formation of A2780T cells.LCC exposure reduced the expression of cancer stemness markers,ALDH1,Myd88 and CD44,while promoting that of terminal differentiation markers,NFATc1,Cathepsin K and MMP9.RNAseq analysis revealed that the expressions of FOS and JUN were upregulated in LCC-treated A2780T cells.A2780T cells overexpressing Fos gene displayed increased paclitaxel-sensitivity and reduced cell stemness,and shared common phenotypes with LCC-treated A2780T cells.Conclusion:These findings suggested that LCC promoted terminal differentiations of ovarian cancer cells and sensitized them to paclitaxel through activating the Fos/Jun pathway.LCC might become a novel therapy that targets at cancer stem cells and enhances the chemotherapeutic effect of ovarian cancer treatments.

HPLC法对细梗香草中槲皮素和山奈酚含量的测定

测定了细梗香草（Lysimachia capillipes HemsL）中槲皮素和山奈酚含量。用甲醇加热回流盐酸水解法，薄层色谱对细梗香草提取液中槲皮素和山奈酚进行定性检验：高效液相色谱法（HPLC）同时测定细梗香草中槲皮素和山奈酚含量，色谱条件为ACI C18（250mm×4．6mm）色谱柱，流动相为甲醇-0．4％磷酸溶液（60：40，V／V），流速为1．0mL／min，检测波长为368nm，柱温为30℃。槲皮素在0．24～2．40μg内线性关系良好，回归方程为y=0．0003x-0．0061，R^2为0．9978，平均回收率为95．91％；山奈酚在0．72-7．20μg内线性关系良好，回归方程为y=0．0002x＋0．4115，R^2为0．9972，平均回收率为103．06％。细梗香草中槲皮素和山奈酚含量在分别为0．021％与0．065％。

药用植物金果榄组织培养初探

以金果榄(Tinospora capillipes Gagnep.)幼嫩茎段为外植体,添加不同浓度的6-苄基腺嘌呤(6-BA)、吲哚-3-丁酸(IBA)、萘乙酸(NAA)、激动素(KT)、赤霉素(GA),研究不同培养基对金果榄试管苗诱导、增殖和生根的影响。结果表明,金果榄启动培养的最佳培养基为MS+1.0 mg/L 6-BA+0.5 mg/L KT+0.1 mg/L 2,4-D,腋芽诱导率达95.1%;增殖的最佳培养基为MS+0.5 mg/L 6-BA+0.2 mg/L GA+0.2 mg/L NAA,增殖系数达到16.1;生根的最佳培养基为1/2MS+0.2 mg/L IBA+0.4 mg/L NAA,30 d时生根率为85.4%,10 d后炼苗移栽到塘泥和河沙(3∶1,体积比)的混合基质中,30 d后移栽成活率可达87.3%以上。