Nitrate transporters(NRTs)are regulators of nitrate assimilation and transport.The genome sequences of TaNRT2L12-A,-B and-D were cloned from wheat(Triticum aestivum L.),and polymorphisms were analyzed by sequencing.Ta...Nitrate transporters(NRTs)are regulators of nitrate assimilation and transport.The genome sequences of TaNRT2L12-A,-B and-D were cloned from wheat(Triticum aestivum L.),and polymorphisms were analyzed by sequencing.TaNRT2L12-D in a germplasm population was highly conserved.However,38 single nucleotide polymorphisms(SNPs)in TaNRT2L12-A coding region and 11 SNPs in TaNRT2L12-B coding region were detected.Two derived cleaved amplified polymorphic sequences(dCAPS)markers A-CSNP1 and A-CSNP2 were developed for TaNRT2L12-A based on SNP-351 and SNP-729,and three haplotypes were identified in the germplasm population.B-CSNP1 and B-CSNP2 were developed for TaNRT2L12-B based on SNP-237 and SNP-1227,and three haplotypes were detected in the germplasm population.Association analyses between the markers and agronomic traits in 30 environments and phenotypic comparisons revealed that A-CSNP2-A is a superior allele of shorter plant height(PH),length of penultimate internode(LPI)and peduncle length(PL),B-CSNP2-G is a superior allele of higher grain number per spike(GNS).Hap-6B-1 containing both superior alleles B-CSNP1-C and B-CSNP2-A is a superior haplotype of 1 OOO-grain weight(TGW).Expression analysis showed that TaNRT2L12-B is mainly expressed in the root base and regulated by nitrate.Therefore,TaNRT2L12 may be involved in nitrate transport and signaling to regulate TGW in wheat.The superior alleles and dCAPS markers of TaNRT2L12-A/B are beneficial to genetic improvement and germplasm enhancement with molecular markers-assisted selection.展开更多
Grh2, a green rice leafhopper resistant gene from an indica cultivar DV85, was located on chromosome 11, and two RFLP markers C189 and G1465 were found to be linked to this gene. In order to transfer Grh2 into Taichun...Grh2, a green rice leafhopper resistant gene from an indica cultivar DV85, was located on chromosome 11, and two RFLP markers C189 and G1465 were found to be linked to this gene. In order to transfer Grh2 into Taichung65, a japonica cultivar with elite characters, backcross method with Taichung65 as the recurrent parent was used and the two RFLP markers were converted into CAPS markers for marker assisted selection (MAS). In the BC6F3 population, both phenotypic evaluation and MAS were conducted to screen the resistant plants with Taichung65 background. The linkage distance between CAPS markers and Grh2 was calculated and the efficiency of MAS was analyzed.展开更多
The idea behind this study is to show that using high yield genes from a wild tomato can enrich tomato breeding resources and accelerate tomato breeding programs. In this study, the near-isogenic line TA1229 containin...The idea behind this study is to show that using high yield genes from a wild tomato can enrich tomato breeding resources and accelerate tomato breeding programs. In this study, the near-isogenic line TA1229 containing a 24-cM introgression at the bottom of chromosome 1 from Lycopersicon acc. LA1777, affects several higher yield traits. The TA1229×9706 BC1 population was analyzed by marker-assisted selection and the traits of the population were evaluated. Twenty-three recombinant individuals that carried a shorter segment than TA1229 were obtained. Among them, 16 lines with the chromosome 1 recombinant segment can increase tomato yield and a QTL affecting yield was found between TG53 and TG158. Sixteen recombinant lines are useful to improve the tomato variety.展开更多
Resistance to whitebacked planthopper(WBPH)in Chinese japonica riceChunjiang 06(CJ-06)was mediated by sucking inhibitory and ovicidal mecha-nism.The ovicidal reponse was a common self-defense mechanism againstWBPH in ...Resistance to whitebacked planthopper(WBPH)in Chinese japonica riceChunjiang 06(CJ-06)was mediated by sucking inhibitory and ovicidal mecha-nism.The ovicidal reponse was a common self-defense mechanism againstWBPH in japonica.The ovicidal gene and its chromosomal position had alreadybeen identified.The sucking inhibitory nature of CJ-06 caused a definenon-preference behavior of WBPH in fields.A single dominant gene governed展开更多
基金This work was supported by the National Key Research and Development Program of China(2017YFD0300202)and the Agricultural Science and Tech no logy Inno vati on Program of Chinese Academy of Agricultural Sciences(ASTIP).
文摘Nitrate transporters(NRTs)are regulators of nitrate assimilation and transport.The genome sequences of TaNRT2L12-A,-B and-D were cloned from wheat(Triticum aestivum L.),and polymorphisms were analyzed by sequencing.TaNRT2L12-D in a germplasm population was highly conserved.However,38 single nucleotide polymorphisms(SNPs)in TaNRT2L12-A coding region and 11 SNPs in TaNRT2L12-B coding region were detected.Two derived cleaved amplified polymorphic sequences(dCAPS)markers A-CSNP1 and A-CSNP2 were developed for TaNRT2L12-A based on SNP-351 and SNP-729,and three haplotypes were identified in the germplasm population.B-CSNP1 and B-CSNP2 were developed for TaNRT2L12-B based on SNP-237 and SNP-1227,and three haplotypes were detected in the germplasm population.Association analyses between the markers and agronomic traits in 30 environments and phenotypic comparisons revealed that A-CSNP2-A is a superior allele of shorter plant height(PH),length of penultimate internode(LPI)and peduncle length(PL),B-CSNP2-G is a superior allele of higher grain number per spike(GNS).Hap-6B-1 containing both superior alleles B-CSNP1-C and B-CSNP2-A is a superior haplotype of 1 OOO-grain weight(TGW).Expression analysis showed that TaNRT2L12-B is mainly expressed in the root base and regulated by nitrate.Therefore,TaNRT2L12 may be involved in nitrate transport and signaling to regulate TGW in wheat.The superior alleles and dCAPS markers of TaNRT2L12-A/B are beneficial to genetic improvement and germplasm enhancement with molecular markers-assisted selection.
基金This work was conducted in Kyushu University,Japan by the first author during his visiting research supported by China Scholarship Counsel(CSC),the“948”Project of the Ministry of Agriculture of Chinathe Program for Outstanding Teachers by the Ministry of Education of China.
文摘Grh2, a green rice leafhopper resistant gene from an indica cultivar DV85, was located on chromosome 11, and two RFLP markers C189 and G1465 were found to be linked to this gene. In order to transfer Grh2 into Taichung65, a japonica cultivar with elite characters, backcross method with Taichung65 as the recurrent parent was used and the two RFLP markers were converted into CAPS markers for marker assisted selection (MAS). In the BC6F3 population, both phenotypic evaluation and MAS were conducted to screen the resistant plants with Taichung65 background. The linkage distance between CAPS markers and Grh2 was calculated and the efficiency of MAS was analyzed.
基金supported by the Natural Science Foundation of Beijing(6042023)the Key Laboratory of Vegetable Genetics and Physiology,Ministry of Agriculture,China.
文摘The idea behind this study is to show that using high yield genes from a wild tomato can enrich tomato breeding resources and accelerate tomato breeding programs. In this study, the near-isogenic line TA1229 containing a 24-cM introgression at the bottom of chromosome 1 from Lycopersicon acc. LA1777, affects several higher yield traits. The TA1229×9706 BC1 population was analyzed by marker-assisted selection and the traits of the population were evaluated. Twenty-three recombinant individuals that carried a shorter segment than TA1229 were obtained. Among them, 16 lines with the chromosome 1 recombinant segment can increase tomato yield and a QTL affecting yield was found between TG53 and TG158. Sixteen recombinant lines are useful to improve the tomato variety.
文摘Resistance to whitebacked planthopper(WBPH)in Chinese japonica riceChunjiang 06(CJ-06)was mediated by sucking inhibitory and ovicidal mecha-nism.The ovicidal reponse was a common self-defense mechanism againstWBPH in japonica.The ovicidal gene and its chromosomal position had alreadybeen identified.The sucking inhibitory nature of CJ-06 caused a definenon-preference behavior of WBPH in fields.A single dominant gene governed
基金supported by the National High Technology Research and Development Program of China (Grant No. 2011AA10A101 and 2012AA101102)the Ministry of Finance, China (Grant No. 2012RG002-4)
