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Research Status of CAR-T Cell Immunotherapy in Tumor Treatment
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作者 Sengthong Mounphoumi Ke Cao +1 位作者 Guangnan Liu Jianing Fang 《Journal of Biosciences and Medicines》 CAS 2023年第3期24-36,共13页
In recent years, chimeric antigen receptor T-cell (CAR-T) therapy has made breakthroughs in the treatment of hematological tumors. However, due to the different characteristics of solid tumors from hematological tumor... In recent years, chimeric antigen receptor T-cell (CAR-T) therapy has made breakthroughs in the treatment of hematological tumors. However, due to the different characteristics of solid tumors from hematological tumors, CAR-T has not achieved good efficacy in the treatment of solid tumors. The key factors limiting the efficacy of CAR-T mainly include the solid tumor cells themselves and their special tumor microenvironment (TME), which damage CAR-T function in multiple processes such as CAR-T infiltration to tumor tissue sites, CAR-T maintaining anti-tumor activity in TME, and target recognition and killing of tumor cells by CAR-T. To solve these problems, more and more preclinical studies have proposed potentially effective solutions, and corresponding clinical studies have been carried out one after another. In this article, the existing challenges and corresponding optimization strategies of CAR-T cell therapy for solid tumors will be reviewed, to provide a reference for the future exploration of CAR-T therapy. 展开更多
关键词 car-t TUMOR cell Therapy IMMUNOTHERAPY
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Insights gained from single-cell analysis of chimeric antigen receptor T-cell immunotherapy in cancer
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作者 Lu Tang Zhong-Pei Huang +1 位作者 Heng Mei Yu Hu 《Military Medical Research》 SCIE CAS CSCD 2024年第5期717-746,共30页
Advances in chimeric antigen receptor(CAR)-T cell therapy have significantly improved clinical outcomes of patients with relapsed or refractory hematologic malignancies.However,progress is still hindered as clinical b... Advances in chimeric antigen receptor(CAR)-T cell therapy have significantly improved clinical outcomes of patients with relapsed or refractory hematologic malignancies.However,progress is still hindered as clinical benefit is only available for a fraction of patients.A lack of understanding of CAR-T cell behaviors in vivo at the single-cell level impedes their more extensive application in clinical practice.Mounting evidence suggests that single-cell sequencing techniques can help perfect the receptor design,guide gene-based T cell modification,and optimize the CAR-T manufacturing conditions,and all of them are essential for long-term immunosurveillance and more favorable clinical outcomes.The information generated by employing these methods also potentially informs our understanding of the numerous complex factors that dictate therapeutic efficacy and toxicities.In this review,we discuss the reasons why CAR-T immunotherapy fails in clinical practice and what this field has learned since the milestone of single-cell sequencing technologies.We further outline recent advances in the application of single-cell analyses in CAR-T immunotherapy.Specifically,we provide an overview of single-cell studies focusing on target antigens,CAR-transgene integration,and preclinical research and clinical applications,and then discuss how it will affect the future of CAR-T cell therapy. 展开更多
关键词 Single-cell sequencing Cancer immunotherapy car-t therapy cell heterogeneity Trajectory inference Tumor microenvironment
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Empowering the Future: Exploring the Construction and Characteristics of Lithium-Ion Batteries
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作者 Dan Tshiswaka Dan 《Advances in Chemical Engineering and Science》 CAS 2024年第2期84-111,共28页
Lithium element has attracted remarkable attraction for energy storage devices, over the past 30 years. Lithium is a light element and exhibits the low atomic number 3, just after hydrogen and helium in the periodic t... Lithium element has attracted remarkable attraction for energy storage devices, over the past 30 years. Lithium is a light element and exhibits the low atomic number 3, just after hydrogen and helium in the periodic table. The lithium atom has a strong tendency to release one electron and constitute a positive charge, as Li<sup> </sup>. Initially, lithium metal was employed as a negative electrode, which released electrons. However, it was observed that its structure changed after the repetition of charge-discharge cycles. To remedy this, the cathode mainly consisted of layer metal oxide and olive, e.g., cobalt oxide, LiFePO<sub>4</sub>, etc., along with some contents of lithium, while the anode was assembled by graphite and silicon, etc. Moreover, the electrolyte was prepared using the lithium salt in a suitable solvent to attain a greater concentration of lithium ions. Owing to the lithium ions’ role, the battery’s name was mentioned as a lithium-ion battery. Herein, the presented work describes the working and operational mechanism of the lithium-ion battery. Further, the lithium-ion batteries’ general view and future prospects have also been elaborated. 展开更多
关键词 Lithium-Ion Batteries Battery construction Battery Characteristics Energy Storage Electrochemical cells Anode Materials Cathode Materials State of Charge (SOC) Depth of Discharge (DOD) Solid Electrolyte Interface (SEI)
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Electricity generation during wastewater treatment by a microbial fuel cell coupled with constructed wetland 被引量:13
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作者 李先宁 宋海亮 +1 位作者 项文力 吴磊 《Journal of Southeast University(English Edition)》 EI CAS 2012年第2期175-178,共4页
A membrane-less constructed wetland microbial fuel cell (CW-MFC) is constructed and operated under continuous flow with a hydraulic retention time (HRT) of 2 d. Fed with glucose, the CW-MFC generates a stable curr... A membrane-less constructed wetland microbial fuel cell (CW-MFC) is constructed and operated under continuous flow with a hydraulic retention time (HRT) of 2 d. Fed with glucose, the CW-MFC generates a stable current density of over 2 A/m3 with a resistor of 1 kΩ and has a chemical oxygen demand (COD) removal efficiency of more than 90% after the startup of 2 to 3 d. A series of systems with the electrode spacings of 10, 20, 30 and 40 cm are compared. It is found that the container with the electrode spacing of 20 cm gains the highest voltage of 560 mV, the highest power density of 0. 149 W/m 3, and the highest Coulombic efficiency of 0.313%. It also has the highest COD removal efficiency of 94. 9%. In addition, the dissolved oxygen (DO) concentrations are observed as the lowest level in the middle of all the CW-MFC reactors. The results show that the more COD is removed, the greater power is generated, and the relatively higher Coulombic efficiency will be achieved. The present study indicates that the CW-MFC process can be used as a cost-effective and environmentally friendly wastewater treatment with simultaneous power generation. 展开更多
关键词 constructed wetland microbial fuel cell wastewater treatment electricity generation electrode spacing
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Culturing adequate CAR-T cells from less peripheral blood to treat B-cell malignancies 被引量:2
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作者 Lu Han Jian Zhou +9 位作者 Linlin Li Keshu Zhou Lingdi Zhao Xinghu Zhu Qingsong Yin Yufu Li Hongqin You Jishuai Zhang Yongping Song Quanli Gao 《Cancer Biology & Medicine》 SCIE CAS CSCD 2021年第4期1066-1079,共14页
Objective:Chimeric antigen receptor-modified T(CAR-T)cells have shown impressive results against relapsed/refractory B cell malignancies.However,the traditional manufacture of CAR-T cells requires leukapheresis to iso... Objective:Chimeric antigen receptor-modified T(CAR-T)cells have shown impressive results against relapsed/refractory B cell malignancies.However,the traditional manufacture of CAR-T cells requires leukapheresis to isolate large amounts of peripheral blood T cells,thus making some patients ineligible for the procedure.Methods:We developed a simple method for CAR-T cell preparation requiring small volumes of peripheral blood.First,CD3+T cells isolated from 50 mL peripheral blood from patients(B-cell malignancies)were stimulated with immobilized anti-CD3/RetroNectin in 6-well plates and then transduced with CAR-expressing lentiviral vector.After 4 d,the T cells were transferred to culture bags for large-scale CAR-T cell expansion.In vitro and animal experiments were performed to evaluate the activity of the manufactured CAR-T cells.Finally,29 patients with B-cell acute lymphoblastic leukemia(B-ALL)and 9 patients with B-cell lymphoma were treated with the CAR-T cells.Results:The CAR-T cells were expanded to 1–3×10^(8) cells in 8–10 d and successfully killed B cell-derived malignant tumor cells in vitro and in vivo.For patients with B-ALL,the complete remission rate was 93%1 month after CAR-T cell infusion;after 12 months,the overall survival(OS)and leukemia-free survival rates were 69%and 31%,respectively.For patients with lymphoma,the objective response rate(including complete and partial remission)was 78%2 months after CAR-T cell infusion,and after 12 months,the OS and progression-free survival rates were 71%and 43%,respectively.Cytokine-release syndrome(CRS)occurred in 65.51%and 55.56%of patients with B-ALL and B-cell lymphoma,respectively;severe CRS developed in 20.69%of patients with B-ALL and in no patients with lymphoma.Conclusions:Our novel method can generate sufficient numbers of CAR-T cells for clinical use from 50–100 mL peripheral blood,thus providing an alternative means of CAR-T cell generation for patients ineligible for leukapheresis. 展开更多
关键词 Fewer initial lymphocytes peripheral blood car-t cells B-cell malignancy acute lymphoblastic leukemia
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Ex vivo 3D osteocyte network construction with primary murine bone cells 被引量:2
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作者 Qiaoling Sun Yexin Gu +3 位作者 Wenting Zhang Leah Dziopa Jenny Zilberberg Woo Lee 《Bone Research》 SCIE CAS CSCD 2015年第3期152-163,共12页
Osteocytes reside as three-dimensionally(3D) networked cells in the lacunocanalicular structure of bones and regulate bone and mineral homeostasis. Despite of their important regulatory roles, in vitro studies of os... Osteocytes reside as three-dimensionally(3D) networked cells in the lacunocanalicular structure of bones and regulate bone and mineral homeostasis. Despite of their important regulatory roles, in vitro studies of osteocytes have been challenging because:(1) current cell lines do not sufficiently represent the phenotypic features of mature osteocytes and(2) primary cells rapidly differentiate to osteoblasts upon isolation. In this study, we used a 3D perfusion culture approach to:(1) construct the 3D cellular network of primary murine osteocytes by biomimetic assembly with microbeads and(2) reproduce ex vivo the phenotype of primary murine osteocytes, for the first time to our best knowledge. In order to enable 3D construction with a sufficient number of viable cells, we used a proliferated osteoblastic population of healthy cells outgrown from digested bone chips. The diameter of microbeads was controlled to:(1) distribute and entrap cells within the interstitial spaces between the microbeads and(2) maintain average cell-to-cell distance to be about 19 mm. The entrapped cells formed a 3D cellular network by extending and connecting their processes through openings between the microbeads. Also, with increasing culture time, the entrapped cells exhibited the characteristic gene expressions(SOST and FGF23) and nonproliferative behavior of mature osteocytes. In contrast, 2D-cultured cells continued their osteoblastic differentiation and proliferation. This 3D biomimetic approach is expected to provide a new means of:(1) studying flow-induced shear stress on the mechanotransduction function of primary osteocytes,(2) studying physiological functions of 3D-networked osteocytes with in vitro convenience,and(3) developing clinically relevant human bone disease models. 展开更多
关键词 cell FIGURE Ex vivo 3D osteocyte network construction with primary murine bone cells BONE
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Construction of Tissue Engineering Artificial Cornea with Skin Stem Cells 被引量:1
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作者 Yuan LIU Yan JIN~(△)(Tissue Engineering Center, Department of Oral Histopathology, The Fourth Military Medical University, Xi’an 710032, China) 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2005年第S1期148-,共1页
关键词 construction of Tissue Engineering Artificial Cornea with Skin Stem cells SFM
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Challenges and interventions of chimeric antigen receptor-T cell therapy in solid tumors 被引量:1
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作者 Shasha Liu Yi Zhang 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2023年第3期239-244,共6页
Adoptive cellular therapy is rapidly improving immunotherapy in hematologic malignancies and several solid tumors.Remarkable clinical success has been achieved in chimeric antigen receptor(CAR)-T cell therapy which re... Adoptive cellular therapy is rapidly improving immunotherapy in hematologic malignancies and several solid tumors.Remarkable clinical success has been achieved in chimeric antigen receptor(CAR)-T cell therapy which represents a paradigm-shifting strategy for the treatment of hematological malignancies.However,many challenges such as resistance,antigen heterogeneity,poor immune cell infiltration,immunosuppressive microenvironment,metabolic obstructive microenvironment,and T cell exhaustion remain as barriers to broader application especially in solid tumors.Encouragingly,the development of new approaches such as multidimensional omics and biomaterials technologies was aided to overcome these barriers.Here,in this perspective,we focus on the most recent clinical advancements,challenges,and strategies of immune cellular therapy in solid tumor treatment represented by CAR-T cell therapy,to provide new ideas to further overcome the bottleneck of immune cell therapy and anticipate future clinical advances. 展开更多
关键词 IMMUNOTHERAPY car-t cell solid tumors METABOLISM
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THE PRINCIPAL CONSTRUCTIVE PROCESS OF UNIT CELL IN Bi-BASED SUPERCONDUCTORS
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作者 Gao Xlaohui Gao De +1 位作者 Gao Yang Jiang Shufen(Department of Chemistry, Central South University of Technology, Changsha, 410083, China ) 《Journal of Central South University》 SCIE EI CAS 1995年第2期1-7,共7页
In this paper, 2201 and 2212 phases as the corresponding precursor of forming 2212 and 2223 phases are discussed,and the constructive process of unit cell of 2201 phase is ascertained by XRD and chemical kinetics me... In this paper, 2201 and 2212 phases as the corresponding precursor of forming 2212 and 2223 phases are discussed,and the constructive process of unit cell of 2201 phase is ascertained by XRD and chemical kinetics method. Subsequently, the process of 221 展开更多
关键词 Bi-based SUPERCONDUCTING PHASES reactional PROCESS construction of unit cell
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Nanotherapeutics approaches to improve the efficacy of CAR-T cells in solid tumors
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作者 FRANCESCO MAININI 《BIOCELL》 SCIE 2021年第5期1171-1173,共3页
Adoptive cell therapy and Immune Checkpoint Blockade Inhibitors have recently revolutionized the field of oncology.However,these types of immunotherapeutic approaches have limited success in treating solid tumors.In p... Adoptive cell therapy and Immune Checkpoint Blockade Inhibitors have recently revolutionized the field of oncology.However,these types of immunotherapeutic approaches have limited success in treating solid tumors.In particular,chimeric antigen receptor(CAR)-T cells efficacy is hampered by immunosuppressive signals in the tumor microenvironment(TME)and by a limited infiltration of re-infused T cells to the tumor site.The field of nanobiotechnology applied to oncology is also rapidly expanding.Nanoparticles-based delivery systems can be employed to modulate the activity of immune cells present in the TME enhancing the efficacy of CAR-T cells.Interestingly,nano-backpacks can be attached to CAR-T cells prior to re-infusion to support their homing to the tumor site and to slowly release immunopotentiators directly in the TME.Furthermore,nanovaccines can also be employed to support the in vivo expansion of CAR-T cells with consequent enhancement of their therapeutic potential.In this viewpoint,recent advancement in the field of nanobiotechnology to support CAR-T cell therapy will be discussed.The development of novel therapeutic CAR-T cells protocols together with nanotherapies is warranted in order to take full advantage of the high therapeutic potential of CAR-T cell therapy. 展开更多
关键词 NANOPARTICLE IMMUNOTHERAPY Cancer Adoptive cell therapy car-t Tumor microenvironme
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Development of a 3D subcutaneous construct containing insulin-producing beta cells using bioprinting
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作者 Chi B.Ahn Ji-Hyun Lee +4 位作者 Joo H.Kim Tae H.Kim Hee-Sook Jun Kuk H.Son Jin W.Lee 《Bio-Design and Manufacturing》 SCIE EI CAS CSCD 2022年第2期265-276,共12页
Type 1 diabetes is caused by insulin deficiency due to the loss of beta cells in the islets of Langerhans.In severe cases,islet transplantation into the portal vein is performed.However,due to the loss of transplanted... Type 1 diabetes is caused by insulin deficiency due to the loss of beta cells in the islets of Langerhans.In severe cases,islet transplantation into the portal vein is performed.However,due to the loss of transplanted islets and the failure of islet function,the 5-year insulin independence rate of these patients is<50%.In this study,we developed a long-term,insulin-secreting,3 Dbioprinted construct implanted subcutaneously with the aim of preventing islet loss.The bioprinted construct was fabricated by the multi-layer bioprinting of beta-cell(mouse insulinoma-6:MIN-6)-encapsulated alginate bioink and poly(caprolactone)biodegradable polymer.A glucose response assay revealed that the bioprinted constructs proliferated and released insulin normally during the 4-week in vitro period.Bioprinted MIN-6 generated clusters with a diameter of 100-200μm,similar to the original pancreatic islets in the construct.In an in vivo study using type 1 diabetes mice,animals implanted with bioprinted constructs showed three times higher insulin secretion and controlled glucose levels at 8 weeks after implantation.Because the implanted,bioprinted constructs had a positive effect on insulin secretion in the experimental animals,the survival rate of the implanted group(75%)was three times higher than that of the non-implanted group(25%).The results suggest that the proposed,3 D-bioprinted,subcutaneous construct can be a better alternative to portal vein islet transplantation. 展开更多
关键词 3D bioprinting Subcutaneous construct Type 1 diabetes INSULIN Beta cells
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Studies on the Construction Parameter of an Artificial Occluded Cell for In-situ Inspection of the Propagation Rate of Localized Corrosion
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作者 Liangcai LEI, Fengping WANG, Yanmin GAO and Yuanlong DU State Key Lab for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China To whom correspondence should be addressed Present address: Department of 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2001年第3期355-358,共4页
An artificial localized corrosion system is assembled and some parameters related to the localized corrosion in active dissolution state (i.e., non-passive state) have been studied. The results showed that the develop... An artificial localized corrosion system is assembled and some parameters related to the localized corrosion in active dissolution state (i.e., non-passive state) have been studied. The results showed that the developed electrochemical system can satisfactorily imitate a naturally formed localized corrosion and the coupling current can indicate the maximum localized propagating rate. In this artificial system, the anodic dissolution reaction followed the auto-catalytic mechanism. The localized corrosion current density was dependent on the area ratio R of the cathode to the occluded anode. While R was equal to or more than 6, the coupling current reached at a maximum value and did not alter with the increase in R-value. Therefore, R=7 is chosen as one of these optimum parameters used in constructing the system, with which the biggest galvanic current might be obtained. In contrast, the thickness of the polymer filler separating the occluded anode area from the bulk electrolyte solution and the volume of the occluded anode area did not affect the corrosion current obviously. They might affect the response time to approach a steady state. 展开更多
关键词 In cell Studies on the construction Parameter of an Artificial Occluded cell for In-situ Inspection of the Propagation Rate of Localized Corrosion UNS
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Construction of the human miRNA-451 expression vector and its expression in gastric carcinoma cell line SGC-7901
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作者 Biao Chen Ximing Xu 《The Chinese-German Journal of Clinical Oncology》 CAS 2013年第8期379-384,共6页
Objective: The aim of the study was to construct miRNA-451 expression vector pLMP-miRNA-451 which could help identify the functions of miRNA-451 in SGC-7901 cell. Methods: Total RNA was extracted from SGC-7901 cells... Objective: The aim of the study was to construct miRNA-451 expression vector pLMP-miRNA-451 which could help identify the functions of miRNA-451 in SGC-7901 cell. Methods: Total RNA was extracted from SGC-7901 cells to synthesized cDNA. The synthesized cDNA encoding pre-miRNA-451 was amplified by polymerase chain reaction (PCR). The PCR product was separated by electrophoresis on 1% agarose gel and then recovered and purified. The purified cDNA fragments of miRNA-451 precursor sequence was then ligated with vector pLMP for 1 h by using DNA ligase to form pLMP- miRNA-451 plasmid. After that, the pLMP-miRNA-451 plasmid was transformed into E. coli DH5a strain expression system to clone and amplificate. The purified pLMP-miRNA-451 extracted from E. coli DH5a via transformation and clone screening was identificatied with restriction enzyme digestion and DNA sequencing. At last, pLMP-miRNA-451 was transfected into SGC-7901 cells with lip2000. Real-time PCR was used for detection of the miRNA-451, the transfection efficiency was ob- served under fluorescence microscopy and cell counting kit-8 assay was conduced to evaluate the effect of miRNA-451 on SGC-7901 cell proliferation. Results: Our results showed that pLMP-miRNA-451 expression vector was not only constructed successfully and effectively infected SGC-7901 cells, but also could repress the SGC-7901 cell proliferation. Conclusion: The constructed plasmid pLMP-miRNA-451 could used for further studies of miRNA-451 in SGC-7901 cell lines. 展开更多
关键词 miRNA-451 SGC-7901 cell construction of pLMP-ha-miRNA-451 transformation and amplification identifica-tion and expression
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Realization Feature of Mesenchymal Dermal Cells Tissue Engineering Construction Response in Granulating Wound Transplantation in Relation with Time-Frame
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作者 Elena Petersen 《Journal of Cosmetics, Dermatological Sciences and Applications》 2012年第3期126-129,共4页
Derma is progenitor cells sours, that are able to differentiate further in several mesodermal lineage and neural and endodermal lineage. Culture conditions, skin taking site and culture medium composition considerably... Derma is progenitor cells sours, that are able to differentiate further in several mesodermal lineage and neural and endodermal lineage. Culture conditions, skin taking site and culture medium composition considerably contribute to it. Spheroid cultured mesenchymal dermal cells contribution to skin regeneration in granulating wound in rat model was estimated. 展开更多
关键词 Brief Report 3D CULTIVATED Tissue Engineering constructION Skin MESENCHYMAL DERMAL cellS SPROUTING Capillary-Like Structures
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Construction of Cox7a2 fluorescent vector and its effect on cytochrome C oxidase activity in mouse Sertoli cell line TM4
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作者 刘保兴 《外科研究与新技术》 2011年第4期260-260,共1页
Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell l... Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell line TM4 by RT-PCR. PCR product was 展开更多
关键词 line cell construction of Cox7a2 fluorescent vector and its effect on cytochrome C oxidase activity in mouse Sertoli cell line TM4 TM
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Construction and screening of suppression subtractive hybridization library of renal cell carcinoma
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作者 张勇 《外科研究与新技术》 2003年第2期114-114,共1页
Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-... Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-2 ( driver), respectiely. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit ( Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F’. All positive clones picked out were digested and some of which were sequenced. Results The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly,2 represented unknown genes and the other 48 derived from 36 known genes. Conclusion The quality of the SSH library of human RCC is reliable and is construction is the basis for further screening differentially expressed genes of RCC. 6 refs,4 figs, 1 tab. 展开更多
关键词 of construction and screening of suppression subtractive hybridization library of renal cell carcinoma
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“植物细胞工程”内容梳理及教学设计探索——基于生活实例与领域科研前沿成果
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作者 齐凤慧 由香玲 范桂枝 《教育教学论坛》 2024年第7期97-100,共4页
植物细胞工程是植物生物技术领域中应用最广泛、取得多项里程碑式成果的一门技术。根据多年的教学实践,提出了一些教学体验。课程内容建设上,围绕“一个总则、两个方面”展开,给学生总结了一个线索图,使他们更好地理解章节之间的逻辑关... 植物细胞工程是植物生物技术领域中应用最广泛、取得多项里程碑式成果的一门技术。根据多年的教学实践,提出了一些教学体验。课程内容建设上,围绕“一个总则、两个方面”展开,给学生总结了一个线索图,使他们更好地理解章节之间的逻辑关系。讲课方式上,根据该课程的特点本着“生活中的植物细胞工程、科研中的植物细胞工程”原则进行讲述,既让学生感到植物细胞的成果就在身边,又通过生物技术领域的最新研究成果,开阔学生的视野,加深对理论的理解,激发学生的学习兴趣。 展开更多
关键词 植物细胞工程 线索图 课程建设
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黄铁矿基MFC-CW耦合系统反硝化动力学研究
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作者 张倩倩 鲁汭 +3 位作者 安恒 卢欣怡 吴振斌 肖恩荣 《水生生物学报》 CAS CSCD 北大核心 2024年第7期1214-1224,共11页
研究比较了黄铁矿基双阳极MFC-CW在不同碳氮比(0和2.5)及初始硝酸盐浓度(7、14和28 mg/L)条件下上阳极和下阳极的反硝化速率,以及对不同阶段硝酸盐还原反应动力学的模拟,从动力学角度揭示系统自养-异养协同反硝化机理。结果显示:不同碳... 研究比较了黄铁矿基双阳极MFC-CW在不同碳氮比(0和2.5)及初始硝酸盐浓度(7、14和28 mg/L)条件下上阳极和下阳极的反硝化速率,以及对不同阶段硝酸盐还原反应动力学的模拟,从动力学角度揭示系统自养-异养协同反硝化机理。结果显示:不同碳氮比下系统两阳极硝酸盐还原效果差异不大,而亚硝酸盐累积、硫酸盐生成的差别较大,两阳极处微生物群落组成相似,优势菌属的相对丰度受C/N、阳极位置影响较大;两阳极处的硝酸盐还原动力学均属于一级反应,且C/N=0时反硝化速率常数(0.0087、0.0045和0.0188/h)均小于C/N=2.5(0.0151、0.0071和0.0798/h;以上阳极为例);MFC-CW系统的反硝化动力学更符合Monod-CSTR模型,且在停留时间较长时取得更好的拟合效果,随着停留时间的增加,C/N=0时系统的反硝速率增加,C/N=2.5时系统的反硝化速率在一定范围内波动[0.6662—0.7744 g/(m^(2)·d)]。实验结果可为黄铁矿基MFC-CW的实际工程应用提供理论指导。 展开更多
关键词 黄铁矿 低碳氮比污水 微生物燃料电池耦合人工湿地 反硝化动力学 混养反硝化
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表达SFTSV Gn基因的重组5型腺病毒的构建与鉴定
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作者 张璇 王新宇 +4 位作者 刘宇婷 涂影叶 梁耀文 易昌华 殷国平 《黑龙江畜牧兽医》 CAS 北大核心 2024年第9期8-12,17,共6页
为了构建出表达新布尼亚病毒(SFTSV)Gn基因的重组5型腺病毒,试验根据GenBank上发表的SFTSV Gn基因序列(登录号为ADZ04482.1)设计合成引物,采用特异性PCR方法在Gn基因前添加了KOZAK序列和tPA信号肽序列;然后通过酶切、连接等方法构建重... 为了构建出表达新布尼亚病毒(SFTSV)Gn基因的重组5型腺病毒,试验根据GenBank上发表的SFTSV Gn基因序列(登录号为ADZ04482.1)设计合成引物,采用特异性PCR方法在Gn基因前添加了KOZAK序列和tPA信号肽序列;然后通过酶切、连接等方法构建重组穿梭质粒PGA-KOZAK-tPA-Gn,与腺病毒骨架质粒pAd5-ΔE1ΔE3-5E4在BJ5183感受态细胞中进行同源重组,得到重组腺病毒质粒rAd5-KOZAK-tPA-Gn,用PacⅠ酶酶切重组腺病毒质粒,并将线性化的质粒转染至HEK 293细胞中进行病毒包装、扩繁和纯化;采用PCR扩增、Western-blot等技术检测病毒基因的表达情况,并测定重组腺病毒效价。结果表明:通过PCR扩增获得了1 546 bp的KOZAK-tPA-Gn基因,并成功构建了重组穿梭质粒;SFTSV Gn基因在重组腺病毒传代过程中稳定存在;重组腺病毒在HEK 293细胞中表达出分子量约为61 ku的SFTSV Gn蛋白;测得重组腺病毒效价为1×10^(-7.63)/0.1 mL TCID_(50)。说明试验成功构建出表达SFTSV Gn基因的重组5型腺病毒。 展开更多
关键词 新布尼亚病毒 Gn基因 重组5型腺病毒 质粒构建 HEK 293细胞
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多源分布式换能系统构建理论方法研究与应用
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作者 孙健 吴可欣 +3 位作者 王寅武 郝俊红 戈志华 杨勇平 《暖通空调》 2024年第5期72-79,共8页
“双碳”背景下分布式换能系统以其在低碳环保方面的显著优势逐渐在集中供热领域得到应用。本文针对分布式换能系统中热泵、太阳能集热器、板式换热器和燃料电池等关键单元提出了单元能耗评价方法,并通过EES软件对各单元进行了热力学建... “双碳”背景下分布式换能系统以其在低碳环保方面的显著优势逐渐在集中供热领域得到应用。本文针对分布式换能系统中热泵、太阳能集热器、板式换热器和燃料电池等关键单元提出了单元能耗评价方法,并通过EES软件对各单元进行了热力学建模以定量评价不同单元在不同工况下的经济性,进而提出分布式换能系统的构建方法以获得流程经济性最优化。以此为依据搭建了最优化流程,并以北京地区某小区为例分析了该系统的供暖季性能,最后进行了经济性和碳减排分析。结果表明:室外温度在-10~5℃范围内变化时,该新型分布式换能系统的COP为2.69~4.43,太阳能集热器效率为45.19%~61.87%,燃料电池日发电量为8047.4~32228.0 kW·h;与常规热力站相比,该新型分布式换能系统的运行成本节省率为32.66%~53.92%,碳减排率为69.07%~73.29%。 展开更多
关键词 分布式换能系统 构建方法 经济性 碳减排 供热 热泵 太阳能集热器 燃料电池
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