<正> An experimentally feasible scheme for implementing teleportation of multi-atom cat-like states in cavityQED is proposed.In the scheme the atoms interact simultaneously with a highly detuned cavity mode and ...<正> An experimentally feasible scheme for implementing teleportation of multi-atom cat-like states in cavityQED is proposed.In the scheme the atoms interact simultaneously with a highly detuned cavity mode and are driven bya strong classical field,and the atomic state evolution is independent of cavity field state.Thus the scheme is insensitiveto both the cavity decay and the thermal field,which is of importance from the experimental point of view.All theorthogonal and complete multi-atom GHZ states can be exactly distinguished only by one step,so our scheme can alsobe used for other purposes such as dense coding using multi-atom GHZ states as quantum channels.展开更多
为将猫重组变应原Fel d 1蛋白展示在乙肝病毒核心抗原(HBcAg)病毒样颗粒的表面,本试验将编码Fel d 1蛋白的两个基因chain1和chain2拼接在一起形成重组Fel d 1(rFel d 1),然后插入到HBcAg的c/e1loop区,取代HBcAg c/e1loop区的D78与E83之...为将猫重组变应原Fel d 1蛋白展示在乙肝病毒核心抗原(HBcAg)病毒样颗粒的表面,本试验将编码Fel d 1蛋白的两个基因chain1和chain2拼接在一起形成重组Fel d 1(rFel d 1),然后插入到HBcAg的c/e1loop区,取代HBcAg c/e1loop区的D78与E83之间的氨基酸。经密码子优化后进行全基因合成,成功构建了pET28aHBcAg-rFel d 1原核表达载体,将其转化入大肠杆菌BL21(DE3)中,进行原核诱导表达与Ni-NTA亲和层析纯化,并进行SDS-PAGE电泳、Western blotting和透射电镜检测。结果显示,本试验成功表达了HBcAg-r Fel d 1融合蛋白,并利用镍柱纯化得到了较纯的HBcAg-rFel d 1融合蛋白,进一步利用负染法透射电子显微镜检测到HBcAgrFel d 1融合蛋白呈现病毒样颗粒结构。HBcAg-rFel d 1融合蛋白能自发形成病毒样颗粒结构,为猫过敏症的预防与治疗性疫苗的开发奠定基础。展开更多
Bacterial infection,tissue hypoxia and inflammatory response can hinder the infected wound repair process.To mitigate the above issues,tannic acid-chelated Fe-decorated molybdenum disulfide nanosheets(MoS2@TA/Fe NSs)w...Bacterial infection,tissue hypoxia and inflammatory response can hinder the infected wound repair process.To mitigate the above issues,tannic acid-chelated Fe-decorated molybdenum disulfide nanosheets(MoS2@TA/Fe NSs)with dual enzyme activities were developed and anchored to a multifunctional hydrogel.The hydrogel exhibited excellent antibacterial ability owing to the combined effects of photothermal therapy(PTT),glutathione(GSH)loss,and the peroxidase(POD)-like activity(catalyse H2O_(2)into⋅OH under acid condition)of MoS2@TA/Fe NSs.Benefitting from the catalase(CAT)-like activity,the hydrogel could decompose H2O_(2)into O_(2)at neutral pH to relieve hypoxia and supply adequate O_(2).POD-like activity was mainly attributed to MoS2 NSs,while CAT-like activity was primarily due to TA/Fe complex.Moreover,MoS2@TA/Fe NSs endowed the hydrogel with outstanding anti-oxidant ability to scavenge redundant reactive oxygen species(ROS)and reactive nitrogen species(RNS)under neutral environment to maintain the balance of antioxidant systems and prevent inflammation.In addition,the hydrogel could inhibit the release of inflammatory factors for the anti-inflammatory property of TA.TA retained partial phenolic hydroxyl groups,which cross-linked the nanosheets to the network structure of the hydrogel and promoted the adhesion of hydrogels.Due to the dynamic boron ester bonds between polyvinyl alcohol(PVA),dextran(Dex),MoS2@TA/Fe,and borax,the hydrogel demonstrated fast self-healing and rapid shape adaptability.This shape-adaptable adhesive hydrogel could fill the whole wound and closely contact the wound,ensuring that it achieved its functions with maximum efficiency.The MoS2@TA/Fe nanozyme-anchored multifunctional hydrogel showed high potential for bacteria-infected wound healing.展开更多
基金The project supported by National Natural Science Foundation of China under Grant No. 10374025 and the Scientific Research Fund of the Education Department of Hunan Province under Grant No. 01c260
文摘<正> An experimentally feasible scheme for implementing teleportation of multi-atom cat-like states in cavityQED is proposed.In the scheme the atoms interact simultaneously with a highly detuned cavity mode and are driven bya strong classical field,and the atomic state evolution is independent of cavity field state.Thus the scheme is insensitiveto both the cavity decay and the thermal field,which is of importance from the experimental point of view.All theorthogonal and complete multi-atom GHZ states can be exactly distinguished only by one step,so our scheme can alsobe used for other purposes such as dense coding using multi-atom GHZ states as quantum channels.
文摘为将猫重组变应原Fel d 1蛋白展示在乙肝病毒核心抗原(HBcAg)病毒样颗粒的表面,本试验将编码Fel d 1蛋白的两个基因chain1和chain2拼接在一起形成重组Fel d 1(rFel d 1),然后插入到HBcAg的c/e1loop区,取代HBcAg c/e1loop区的D78与E83之间的氨基酸。经密码子优化后进行全基因合成,成功构建了pET28aHBcAg-rFel d 1原核表达载体,将其转化入大肠杆菌BL21(DE3)中,进行原核诱导表达与Ni-NTA亲和层析纯化,并进行SDS-PAGE电泳、Western blotting和透射电镜检测。结果显示,本试验成功表达了HBcAg-r Fel d 1融合蛋白,并利用镍柱纯化得到了较纯的HBcAg-rFel d 1融合蛋白,进一步利用负染法透射电子显微镜检测到HBcAgrFel d 1融合蛋白呈现病毒样颗粒结构。HBcAg-rFel d 1融合蛋白能自发形成病毒样颗粒结构,为猫过敏症的预防与治疗性疫苗的开发奠定基础。
基金This work was supported by the National Natural Science Foundation of China(grant numbers 21878247)Key Program of the National Natural Science Foundation of China(grant numbers 21838009)+2 种基金National Key Research and Development Program(2019YFA0905200)and Xi’an Science and Technology Project(20191422315KYPT014JC016)The authors thank Dr.J.C.Li(School of Chemical Engineering,Northwest University,Xi’an,China)for improving the manuscript during revising.
文摘Bacterial infection,tissue hypoxia and inflammatory response can hinder the infected wound repair process.To mitigate the above issues,tannic acid-chelated Fe-decorated molybdenum disulfide nanosheets(MoS2@TA/Fe NSs)with dual enzyme activities were developed and anchored to a multifunctional hydrogel.The hydrogel exhibited excellent antibacterial ability owing to the combined effects of photothermal therapy(PTT),glutathione(GSH)loss,and the peroxidase(POD)-like activity(catalyse H2O_(2)into⋅OH under acid condition)of MoS2@TA/Fe NSs.Benefitting from the catalase(CAT)-like activity,the hydrogel could decompose H2O_(2)into O_(2)at neutral pH to relieve hypoxia and supply adequate O_(2).POD-like activity was mainly attributed to MoS2 NSs,while CAT-like activity was primarily due to TA/Fe complex.Moreover,MoS2@TA/Fe NSs endowed the hydrogel with outstanding anti-oxidant ability to scavenge redundant reactive oxygen species(ROS)and reactive nitrogen species(RNS)under neutral environment to maintain the balance of antioxidant systems and prevent inflammation.In addition,the hydrogel could inhibit the release of inflammatory factors for the anti-inflammatory property of TA.TA retained partial phenolic hydroxyl groups,which cross-linked the nanosheets to the network structure of the hydrogel and promoted the adhesion of hydrogels.Due to the dynamic boron ester bonds between polyvinyl alcohol(PVA),dextran(Dex),MoS2@TA/Fe,and borax,the hydrogel demonstrated fast self-healing and rapid shape adaptability.This shape-adaptable adhesive hydrogel could fill the whole wound and closely contact the wound,ensuring that it achieved its functions with maximum efficiency.The MoS2@TA/Fe nanozyme-anchored multifunctional hydrogel showed high potential for bacteria-infected wound healing.
