Tinnitus can be described as the conscious perception of sound without external stimulation,and it is often accompanied by anxiety,depression,and insomnia.Current clinical treatments for tinnitus are ineffective.Altho...Tinnitus can be described as the conscious perception of sound without external stimulation,and it is often accompanied by anxiety,depression,and insomnia.Current clinical treatments for tinnitus are ineffective.Although recent studies have indicated that the caudateputamen nucleus may be a sensory gating area involved in noise elimination in tinnitus,the underlying mechanisms of this disorder are yet to be determined.To investigate the potential role of the caudate-putamen nucleus in experimentally induced tinnitus,we created a rat model of tinnitus induced by intraperitoneal administration of 350 mg/kg sodium salicylate.Our results revealed that the mean spontaneous firing rate of the caudate-putamen nucleus was increased by sodium salicylate treatment,while dopamine levels were decreased.In addition,electrical stimulation of the caudate-putamen nucleus markedly reduced the spontaneous firing rate of neurons in the primary auditory cortex.These findings suggest that the caudate-putamen nucleus plays a sensory gating role in sodium salicylate-induced tinnitus.This study was approved by the Institutional Animal Care and Use Committee of Peking University Health Science Center(approval No.A2010031)on December 6,2017.展开更多
The effect of the extract from caudate-putamen nucleus of newborn rats(CPe)on thedopaminergic(DA)neurons has been studied.An MTT colorimetric microassay measuring optical densitiesfor the growth of DA neuron cultures ...The effect of the extract from caudate-putamen nucleus of newborn rats(CPe)on thedopaminergic(DA)neurons has been studied.An MTT colorimetric microassay measuring optical densitiesfor the growth of DA neuron cultures reveals a significant increase in growth for cultures with CPe as com-pared to those without CPe(p【0.05).Rhodamine retrograde-prelabelled DA neurons were cultured on aPhastgel containing all the electrophoretically separated protein bands from CPe,and able to fish out from ittheir own trophic factor,a 12.5-kD protein band.The survived neurons at the 12.5-kD protein band wereimmunostained positive with anti-dopamine antibody.Co-culturing the 12.5 kD-containing gel strip with ex-plants from substantia nigra at a close distance for a week revealed anti-dopamine immunopositive neuritesoutgrowing from the explants only towards the 12.5-kD gel strip.These results indicate that a 12.5-kD pro-tein from the CPe is capable of maintaining the survival of DA neurons of the substantia nigra and promotingtheir neurite outgrowth.展开更多
基金supported by the National Natural Science Foundation of China,Nos.21790391(to LQM)and 81870727(to FRM).
文摘Tinnitus can be described as the conscious perception of sound without external stimulation,and it is often accompanied by anxiety,depression,and insomnia.Current clinical treatments for tinnitus are ineffective.Although recent studies have indicated that the caudateputamen nucleus may be a sensory gating area involved in noise elimination in tinnitus,the underlying mechanisms of this disorder are yet to be determined.To investigate the potential role of the caudate-putamen nucleus in experimentally induced tinnitus,we created a rat model of tinnitus induced by intraperitoneal administration of 350 mg/kg sodium salicylate.Our results revealed that the mean spontaneous firing rate of the caudate-putamen nucleus was increased by sodium salicylate treatment,while dopamine levels were decreased.In addition,electrical stimulation of the caudate-putamen nucleus markedly reduced the spontaneous firing rate of neurons in the primary auditory cortex.These findings suggest that the caudate-putamen nucleus plays a sensory gating role in sodium salicylate-induced tinnitus.This study was approved by the Institutional Animal Care and Use Committee of Peking University Health Science Center(approval No.A2010031)on December 6,2017.
基金the University of Hong Kong and the Growth Factor
文摘The effect of the extract from caudate-putamen nucleus of newborn rats(CPe)on thedopaminergic(DA)neurons has been studied.An MTT colorimetric microassay measuring optical densitiesfor the growth of DA neuron cultures reveals a significant increase in growth for cultures with CPe as com-pared to those without CPe(p【0.05).Rhodamine retrograde-prelabelled DA neurons were cultured on aPhastgel containing all the electrophoretically separated protein bands from CPe,and able to fish out from ittheir own trophic factor,a 12.5-kD protein band.The survived neurons at the 12.5-kD protein band wereimmunostained positive with anti-dopamine antibody.Co-culturing the 12.5 kD-containing gel strip with ex-plants from substantia nigra at a close distance for a week revealed anti-dopamine immunopositive neuritesoutgrowing from the explants only towards the 12.5-kD gel strip.These results indicate that a 12.5-kD pro-tein from the CPe is capable of maintaining the survival of DA neurons of the substantia nigra and promotingtheir neurite outgrowth.
