BACKGROUND Gastrointestinal stromal tumors(GISTs)are the most common mesenchymal neoplasms of the gastrointestinal tract.Surgical resection and tyrosine kinase inhibitors are defined as the main treatments but cannot ...BACKGROUND Gastrointestinal stromal tumors(GISTs)are the most common mesenchymal neoplasms of the gastrointestinal tract.Surgical resection and tyrosine kinase inhibitors are defined as the main treatments but cannot cure patients with advanced GIST,which eventually develops into recurrence and acquired drug resistance.Therefore,it is necessary to identify prognostic biomarkers and new therapeutic targets for GISTs.CC chemokine receptor type 8(CCR8)protein participates in regulation of immune responses.Recent studies on CCR8 in nonsmall cell lung cancer and colorectal cancer showed that it was highly expressed in tumor-infiltrating regulatory T cells and correlated with a poor prognosis.AIM To detect CCR8 expression in GIST tissues and analyze its relationships with clinicopathological features and prognosis in patients with GISTs.METHODS Tissue samples were used for the tissue microarrays construction.The microarrays were then subjected to immunohistochemical analyses to detect CCR8 expression.Next,Kaplan–Meier analysis was utilized to calculate the survival rate of patients with complete follow-up data,and the potential prognostic value of CCR8 was evaluated by Cox regression analysis.Finally,a Gene Ontology/Kyoto Encyclopedia of Genes and Genomes single-gene enrichment chart of CCR8 was constructed using the STRING database.RESULTS CCR8-positive signals were detected as brown or brown-yellow particles by immunohistochemistry located in the cytoplasm.Among 125 tissue samples,74 had CCR8 high expression and 51 had low or negative expression.Statistical analyses suggested CCR8 was significantly correlated with tumor size,mitotic index,AFIP-Miettinen risk classification and tumor location.Kaplan–Meier and multivariate analyses showed that patients with low or negative CCR8 expression,mitotic index<5/high-power fields(HPF)and tumor diameter<5 cm had a better prognosis.Based on the STRING database,CCR8 was significantly enriched in biological processes such as tumor immunity,T lymphocyte chemotaxis,migration and pathways like the nuclear factor-κB and tumor necrosis factor pathways as well as intestinal immune regulation networks.CONCLUSION CCR8 is a prognostic biomarker for malignant potential of GISTs,with high expression correlated with malignancy and poor prognosis.展开更多
Currently, the historical archive images of Landsat family sensors are probably the most effective data products for tracking global longitudinal changes since the 1970 s. However, the issue of the degree and extent o...Currently, the historical archive images of Landsat family sensors are probably the most effective data products for tracking global longitudinal changes since the 1970 s. However, the issue of the degree and extent of cloud coverage is always a challenge and varies distinctively worldwide. So far, acquisition probability（AP） analyses of cloud cover（CC） of Landsat observations have been conducted with different sensors at regional scale. To our knowledge, CC probability analysis for the newly-launched Landsat-8 Operational Land Imager（OLI） across China is not reported. In this paper, monthly, seasonal, and annual APs for Landsat OLI（44,228 in total） images over China acquired from April 2013 to October 2016 with various CC thresholds were analyzed. The results showed that： first, the cumulative average APs of all OLI data over China at the CC thresholds ≤30% was about 49.6% which illustrated the availability of OLI imagery across China. Second, the spatial patterns of 10%, 20%, and 30% CC thresholds of OLI observations, coincided well with the precipitation distributions separated by the respective 200 mm, 400 mm, and 800 mm isohyetal lines. Third, the APs of images with the 30% CC threshold are the highest in autumn and winter especially in October of 58.7%, while the corresponding lowest probability occurred in June of 41.0%. Finally, the spatial differences in APs of targeted images with ≤30% CC thresholds were quite significant. At regional scales, the arid and semi-arid areas, Inland River and Songliao River basins, and northwestern side of the Hu Huanyong population line had the larger probabilities of obtaining high-quality images. Our study suggested that OLI imagery satisfy the data requirements needed for land surface monitoring, although there existed obvious spatio-temporal differences in APs over China at the 30% CC threshold.展开更多
文摘BACKGROUND Gastrointestinal stromal tumors(GISTs)are the most common mesenchymal neoplasms of the gastrointestinal tract.Surgical resection and tyrosine kinase inhibitors are defined as the main treatments but cannot cure patients with advanced GIST,which eventually develops into recurrence and acquired drug resistance.Therefore,it is necessary to identify prognostic biomarkers and new therapeutic targets for GISTs.CC chemokine receptor type 8(CCR8)protein participates in regulation of immune responses.Recent studies on CCR8 in nonsmall cell lung cancer and colorectal cancer showed that it was highly expressed in tumor-infiltrating regulatory T cells and correlated with a poor prognosis.AIM To detect CCR8 expression in GIST tissues and analyze its relationships with clinicopathological features and prognosis in patients with GISTs.METHODS Tissue samples were used for the tissue microarrays construction.The microarrays were then subjected to immunohistochemical analyses to detect CCR8 expression.Next,Kaplan–Meier analysis was utilized to calculate the survival rate of patients with complete follow-up data,and the potential prognostic value of CCR8 was evaluated by Cox regression analysis.Finally,a Gene Ontology/Kyoto Encyclopedia of Genes and Genomes single-gene enrichment chart of CCR8 was constructed using the STRING database.RESULTS CCR8-positive signals were detected as brown or brown-yellow particles by immunohistochemistry located in the cytoplasm.Among 125 tissue samples,74 had CCR8 high expression and 51 had low or negative expression.Statistical analyses suggested CCR8 was significantly correlated with tumor size,mitotic index,AFIP-Miettinen risk classification and tumor location.Kaplan–Meier and multivariate analyses showed that patients with low or negative CCR8 expression,mitotic index<5/high-power fields(HPF)and tumor diameter<5 cm had a better prognosis.Based on the STRING database,CCR8 was significantly enriched in biological processes such as tumor immunity,T lymphocyte chemotaxis,migration and pathways like the nuclear factor-κB and tumor necrosis factor pathways as well as intestinal immune regulation networks.CONCLUSION CCR8 is a prognostic biomarker for malignant potential of GISTs,with high expression correlated with malignancy and poor prognosis.
基金National Natural Science Foundation of China,No.41430861National Key Research and Development Program of China,No.2016YFC0503500Opening Fund of Key Laboratory of Poyang Lake Wetland and Watershed Research,Ministry of Education,Jiangxi Normal University,No.PK2016004
文摘Currently, the historical archive images of Landsat family sensors are probably the most effective data products for tracking global longitudinal changes since the 1970 s. However, the issue of the degree and extent of cloud coverage is always a challenge and varies distinctively worldwide. So far, acquisition probability（AP） analyses of cloud cover（CC） of Landsat observations have been conducted with different sensors at regional scale. To our knowledge, CC probability analysis for the newly-launched Landsat-8 Operational Land Imager（OLI） across China is not reported. In this paper, monthly, seasonal, and annual APs for Landsat OLI（44,228 in total） images over China acquired from April 2013 to October 2016 with various CC thresholds were analyzed. The results showed that： first, the cumulative average APs of all OLI data over China at the CC thresholds ≤30% was about 49.6% which illustrated the availability of OLI imagery across China. Second, the spatial patterns of 10%, 20%, and 30% CC thresholds of OLI observations, coincided well with the precipitation distributions separated by the respective 200 mm, 400 mm, and 800 mm isohyetal lines. Third, the APs of images with the 30% CC threshold are the highest in autumn and winter especially in October of 58.7%, while the corresponding lowest probability occurred in June of 41.0%. Finally, the spatial differences in APs of targeted images with ≤30% CC thresholds were quite significant. At regional scales, the arid and semi-arid areas, Inland River and Songliao River basins, and northwestern side of the Hu Huanyong population line had the larger probabilities of obtaining high-quality images. Our study suggested that OLI imagery satisfy the data requirements needed for land surface monitoring, although there existed obvious spatio-temporal differences in APs over China at the 30% CC threshold.
