The proposed multi-dimensional quantitative fluorescence microscopy for the CASLIBB single-particle microbeam II endstation is a CCD-based imaging system. We systematically analyse the theoretical and the practical co...The proposed multi-dimensional quantitative fluorescence microscopy for the CASLIBB single-particle microbeam II endstation is a CCD-based imaging system. We systematically analyse the theoretical and the practical considerations pertinent to choosing the right CCD camera and unveiling the principles underlying multifarious parameters. Therefore, this analysis can be a valuable tool in scrutinizing each parameter and clarifying proper usage of a scientific CCD camera.展开更多
基金supported by the the National Major Technologies R&D Programme of China during the 10th Five-Year Plan Period(No.2001BA302B)the National Science Foundation for Distinguished Young Scholars(No.10225526)+1 种基金the Knowledge Innovation Programme of the Chinese Academy of Sciences(No.KSCX2-SW-324)the Foundation for University Key Teacher by the Ministry of Education(No.2005jq1135).
文摘The proposed multi-dimensional quantitative fluorescence microscopy for the CASLIBB single-particle microbeam II endstation is a CCD-based imaging system. We systematically analyse the theoretical and the practical considerations pertinent to choosing the right CCD camera and unveiling the principles underlying multifarious parameters. Therefore, this analysis can be a valuable tool in scrutinizing each parameter and clarifying proper usage of a scientific CCD camera.