AIM:To explore whether CD3ε is involved in the adaptive immunity of Aspergillus fumigatus(A.fumigatus)keratitis in mice and the role of innate and adaptive immunity in it.METHODS:Mice models of A.fumigatus keratitis ...AIM:To explore whether CD3ε is involved in the adaptive immunity of Aspergillus fumigatus(A.fumigatus)keratitis in mice and the role of innate and adaptive immunity in it.METHODS:Mice models of A.fumigatus keratitis were established by intra-stromal injection and corneal epithelial scratching.Subconjunctival injections of natamycin,wedelolactone,LOX-1 inhibitor(poly I)or Dectin-1 inhibitor(laminarin)were used to treat mice with A.fumigatus keratitis.Mice were pretreated by intraperitoneal injection of anti-mouse CD3ε.We observed the corneal infection of mice under the slit lamp microscope and made a clinical score.The protein expression of CD3ε and interleukin-10(IL-10)was determined by Western blotting.RESULTS:With the disease progresses,the degree of corneal opacity and edema augmented.In the intrastromal injection models,CD3εprotein expression began to increase significantly on the 2^(nd) day.However,in the scraping epithelial method models,CD3ε only began to increase on the 3^(rd) day.After natamycin treatment,the degree of corneal inflammation in mice was significantly attenuated on the 3^(rd) day.After wedelolactone treatment,the severity of keratitis worsened.And the amount of CD3ε protein was also reduced,compared with the control group.By inhibiting LOX-1 and Dectin-1,there was no significant difference in CD3ε production compared with the control group.After inhibiting CD3ε,corneal ulcer area and clinical score increased,and IL-10 expression was downregulated.CONCLUSION:As a pan T cell marker,CD3ε participate in the adaptive immunity of A.fumigatus keratitis in mice.In our mice models,the corneas will enter the adaptive immune stage faster.By regulating IL-10,CD3ε exerts antiinflammatory and repairs effects in the adaptive immune stage.展开更多
A simple two-step hydrothermal method synthesized four different CdS/Fe_(3)O_(4)photocatalysts with varying ratios of mass of CdS to Fe_(3)O_(4).The composition and morphology of the prepared samples were investigated...A simple two-step hydrothermal method synthesized four different CdS/Fe_(3)O_(4)photocatalysts with varying ratios of mass of CdS to Fe_(3)O_(4).The composition and morphology of the prepared samples were investigated using X-ray diffraction(XRD),Raman spectrum,X-ray photoelectron spectroscopy(XPS),scanning electron microscopy(SEM),and transmission electron microscopy(TEM).Solid UV reflectance spectra testing found that CdS/Fe_(3)O_(4)nanocomposites had good light absorption throughout the spectral range,promoting their photocatalytic properties.Under visible light irradiation,CdS/Fe_(3)O_(4)(2∶5)with a mass ratio of 2∶5 exhibited excellent photocatalytic perfor-mance,with a degradation rate of 98.8%for rhodamine B.Furthermore,after five cycles of photocatalytic degrada-tion reaction,the rhodamine B degradation rate remained at 96.2%,indicating that the photocatalysts have good pho-tocatalytic stability.展开更多
基金Supported by the National Natural Science Foundation of China(No.82171019)the Natural Science Foundation of Shandong Province(No.ZR2021MH368)+1 种基金Traditional Chinese Medicine Research Project of Qingdao(No.2020-zyy055)Shandong Qingdao Outstanding Health Professional Development Fund.
文摘AIM:To explore whether CD3ε is involved in the adaptive immunity of Aspergillus fumigatus(A.fumigatus)keratitis in mice and the role of innate and adaptive immunity in it.METHODS:Mice models of A.fumigatus keratitis were established by intra-stromal injection and corneal epithelial scratching.Subconjunctival injections of natamycin,wedelolactone,LOX-1 inhibitor(poly I)or Dectin-1 inhibitor(laminarin)were used to treat mice with A.fumigatus keratitis.Mice were pretreated by intraperitoneal injection of anti-mouse CD3ε.We observed the corneal infection of mice under the slit lamp microscope and made a clinical score.The protein expression of CD3ε and interleukin-10(IL-10)was determined by Western blotting.RESULTS:With the disease progresses,the degree of corneal opacity and edema augmented.In the intrastromal injection models,CD3εprotein expression began to increase significantly on the 2^(nd) day.However,in the scraping epithelial method models,CD3ε only began to increase on the 3^(rd) day.After natamycin treatment,the degree of corneal inflammation in mice was significantly attenuated on the 3^(rd) day.After wedelolactone treatment,the severity of keratitis worsened.And the amount of CD3ε protein was also reduced,compared with the control group.By inhibiting LOX-1 and Dectin-1,there was no significant difference in CD3ε production compared with the control group.After inhibiting CD3ε,corneal ulcer area and clinical score increased,and IL-10 expression was downregulated.CONCLUSION:As a pan T cell marker,CD3ε participate in the adaptive immunity of A.fumigatus keratitis in mice.In our mice models,the corneas will enter the adaptive immune stage faster.By regulating IL-10,CD3ε exerts antiinflammatory and repairs effects in the adaptive immune stage.
文摘A simple two-step hydrothermal method synthesized four different CdS/Fe_(3)O_(4)photocatalysts with varying ratios of mass of CdS to Fe_(3)O_(4).The composition and morphology of the prepared samples were investigated using X-ray diffraction(XRD),Raman spectrum,X-ray photoelectron spectroscopy(XPS),scanning electron microscopy(SEM),and transmission electron microscopy(TEM).Solid UV reflectance spectra testing found that CdS/Fe_(3)O_(4)nanocomposites had good light absorption throughout the spectral range,promoting their photocatalytic properties.Under visible light irradiation,CdS/Fe_(3)O_(4)(2∶5)with a mass ratio of 2∶5 exhibited excellent photocatalytic perfor-mance,with a degradation rate of 98.8%for rhodamine B.Furthermore,after five cycles of photocatalytic degrada-tion reaction,the rhodamine B degradation rate remained at 96.2%,indicating that the photocatalysts have good pho-tocatalytic stability.
