目的:探讨CD4^(+)/CD8^(+)比值降钙素原(Procallcitonin,PCT)、C反应蛋白(C-reactiveprotein,CRP)水平变化与感染性休克(Septicshock,SS)患者疾病转归的相关性。方法:选取我院2020年4月-2022年2月收治的135例SS患者为研究对象,根据患者...目的:探讨CD4^(+)/CD8^(+)比值降钙素原(Procallcitonin,PCT)、C反应蛋白(C-reactiveprotein,CRP)水平变化与感染性休克(Septicshock,SS)患者疾病转归的相关性。方法:选取我院2020年4月-2022年2月收治的135例SS患者为研究对象,根据患者28d疾病转归分为生存组(91例)和病死组(44例)。比较两组一般临床资料,比较两组治疗前、治疗3d、7d后CD4^(+)/CD8^(+)比值、PCT、CRP水平,分析治疗3d、7d后CD4^(+)/CD8^(+)比值、PCT、CRP水平与急性生理与慢性健康评估(Acute physiology and chronic health evaluationⅡ,APCHEⅡ)评分、序贯器官衰竭(Sepsis-related organ failure assessment,SOFA)评分及疾病转归的相关性,偏回归分析影响SS患者疾病转归的危险因素。结果:病死组年龄、治疗3d后、7d后APACHEⅡ评分、SOFA评分较生存组高(P<0.05);病死组治疗3d、7d后CD4^(+)/CD8^(+)比值低于生存组,PCT、CRP水平高于生存组(P<0.05);治疗3d、7d后,CD4+/CD8+比值与SOFA评分、APCHEⅡ评分、疾病转归呈负相关,PCT、CRP水平与SOFA评分、APCHEⅡ评分、疾病转归呈正相关(P<0.05);Logistic回归分析显示,治疗3d后CD4^(+)/CD8^(+)比值≤1.10、PCT>19.18μg/L、CRP>122.98mg/L,治疗7d后CD4^(+)/CD8^(+)比值≤1.18、PCT>18.03μg/L、CRP>111.65mg/mL是SS患者病死的危险因素(P<0.05)。结论:CD4^(+)/CD8^(+)比值、PCT、CRP水平与SS患者疾病转归密切相关,检测其水平对临床预测患者疾病转归具有指导意义。展开更多
BACKGROUND:The effect of pituitary adenylate cyclase activating polypeptide(PACAP)during traumatic brain injury(TBI) and whether it can modulate secondary injury has not been reported previously.The present study eval...BACKGROUND:The effect of pituitary adenylate cyclase activating polypeptide(PACAP)during traumatic brain injury(TBI) and whether it can modulate secondary injury has not been reported previously.The present study evaluated the potential protective effects of ventricular infusion of PACAP in a rat model of TBI.METHODS:Male Sprague Dawley rats were randomly divided into 3 treatment groups(n=6,each):sham-operated,vehicle(normal saline)^+TBI,and PACAP^+TBI.Normal saline or PACAP(1 ug/5uL) was administered intracerebroventricularly 20 minutes before TBI.Right parietal cortical contusion was produced via a weight-dropping method.Brains were extracted 24 hours after trauma.Histological changes in brains were examined by HE staining.The numbers of CD4^+ and CD8^+ T cells in blood and the spleen were detected via flow cytometry.RESULTS:In injured brain regions,edema,hemorrhage,inflammatory cell infiltration,and swollen and degenerated neurons were observed under a light microscope,and the neurons were disorderly arrayed in the hippocampi.Compared to the sham group,average CD4^+ CD8" lymphocyte counts in blood and the spleen were significantly decreased in rats that received TBI^+vehicle,and CD4^+ CD8^+ were increased.In rats administered PACAP prior to TBI,damage was attenuated as evidenced by significantly increased CD4^+,and decreased CD8^+,T lymphocytes in blood and the spleen.CONCLUSION:Pretreatment with PACAP may protect against TBI by influencing periphery T cellular immune function.展开更多
文摘目的:探讨CD4^(+)/CD8^(+)比值降钙素原(Procallcitonin,PCT)、C反应蛋白(C-reactiveprotein,CRP)水平变化与感染性休克(Septicshock,SS)患者疾病转归的相关性。方法:选取我院2020年4月-2022年2月收治的135例SS患者为研究对象,根据患者28d疾病转归分为生存组(91例)和病死组(44例)。比较两组一般临床资料,比较两组治疗前、治疗3d、7d后CD4^(+)/CD8^(+)比值、PCT、CRP水平,分析治疗3d、7d后CD4^(+)/CD8^(+)比值、PCT、CRP水平与急性生理与慢性健康评估(Acute physiology and chronic health evaluationⅡ,APCHEⅡ)评分、序贯器官衰竭(Sepsis-related organ failure assessment,SOFA)评分及疾病转归的相关性,偏回归分析影响SS患者疾病转归的危险因素。结果:病死组年龄、治疗3d后、7d后APACHEⅡ评分、SOFA评分较生存组高(P<0.05);病死组治疗3d、7d后CD4^(+)/CD8^(+)比值低于生存组,PCT、CRP水平高于生存组(P<0.05);治疗3d、7d后,CD4+/CD8+比值与SOFA评分、APCHEⅡ评分、疾病转归呈负相关,PCT、CRP水平与SOFA评分、APCHEⅡ评分、疾病转归呈正相关(P<0.05);Logistic回归分析显示,治疗3d后CD4^(+)/CD8^(+)比值≤1.10、PCT>19.18μg/L、CRP>122.98mg/L,治疗7d后CD4^(+)/CD8^(+)比值≤1.18、PCT>18.03μg/L、CRP>111.65mg/mL是SS患者病死的危险因素(P<0.05)。结论:CD4^(+)/CD8^(+)比值、PCT、CRP水平与SS患者疾病转归密切相关,检测其水平对临床预测患者疾病转归具有指导意义。
文摘BACKGROUND:The effect of pituitary adenylate cyclase activating polypeptide(PACAP)during traumatic brain injury(TBI) and whether it can modulate secondary injury has not been reported previously.The present study evaluated the potential protective effects of ventricular infusion of PACAP in a rat model of TBI.METHODS:Male Sprague Dawley rats were randomly divided into 3 treatment groups(n=6,each):sham-operated,vehicle(normal saline)^+TBI,and PACAP^+TBI.Normal saline or PACAP(1 ug/5uL) was administered intracerebroventricularly 20 minutes before TBI.Right parietal cortical contusion was produced via a weight-dropping method.Brains were extracted 24 hours after trauma.Histological changes in brains were examined by HE staining.The numbers of CD4^+ and CD8^+ T cells in blood and the spleen were detected via flow cytometry.RESULTS:In injured brain regions,edema,hemorrhage,inflammatory cell infiltration,and swollen and degenerated neurons were observed under a light microscope,and the neurons were disorderly arrayed in the hippocampi.Compared to the sham group,average CD4^+ CD8" lymphocyte counts in blood and the spleen were significantly decreased in rats that received TBI^+vehicle,and CD4^+ CD8^+ were increased.In rats administered PACAP prior to TBI,damage was attenuated as evidenced by significantly increased CD4^+,and decreased CD8^+,T lymphocytes in blood and the spleen.CONCLUSION:Pretreatment with PACAP may protect against TBI by influencing periphery T cellular immune function.