Objective： To investigate the effects of synthetic retinoid CD437 and acitretin on cell proliferation, apoptosis, cycle arrest and Bax/ Bcl-2 protein expression of melanoma A375 cell, Methods：MTT assay was used to d...Objective： To investigate the effects of synthetic retinoid CD437 and acitretin on cell proliferation, apoptosis, cycle arrest and Bax/ Bcl-2 protein expression of melanoma A375 cell, Methods：MTT assay was used to determine the anti-proliferative effects of CD437 and acitretin on melanoma A375 cell, Flow cytometry was performed to investigate the influence of CD437 and acitretin on cell cycle and cell apoptosis. SABC immunocytochemistry was employed for detection of Bax/bcl-2 protein expressions. Results：10^-5 mol/L CD437 was more effective than acitretin in inhibiting proliferation and inducing apoptosis of A375 cell after 24 h treatment, growth inhibiting ratio and apoptosis ratio（58.6%vs43.25% and 28.03%vs17.13%, P 〈 0.05 respectively）. CD437 promoted G0/G1 arrest in melanoma A375 cell, however acitretin could not. CD437 and acitretin could up-regulate the expression of Bax protein and downregulate the expression of bcl-2 protein（P 〈 0.05）. Conclusion：CD437 is more effective than acitretin in inhibiting proliferation and inducing apoptosis and cycle arrest on A375 cell, CD437 may have more potentialities than acitretin for subsidiary treatment of melanoma. Mitochondrial apoptosis pathway is partially involved in two drugs inducing apoptosis on A375 cell.展开更多
This study aimed to investigate the effects of celecoxib,synthetic retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalenecarboxylicacid(CD437)and the combination of the two on cell proliferation,apoptosis,and cycl...This study aimed to investigate the effects of celecoxib,synthetic retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalenecarboxylicacid(CD437)and the combination of the two on cell proliferation,apoptosis,and cycle arrest of human malignant mela-noma A375 cells.3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazoliumbromide assay(MTT assay)was applied to determine the anti-proliferative effects of the drugs on human malignant melanoma A375 cells.Flow cytometry was performed to investigate the influence of the drugs on cell cycle and cell apoptosis.Both celecoxib and CD437 could inhibit the growth of human malignant melanoma A375 cells in a dose-dependent manner.Celecoxib at 80μmol/L inhibited proliferation,induced apoptosis and G2/M cell cycle arrest of human malignant melanoma A375 cells after treatment for 24 h[proliferation inhibiting rate:(50.2±2.51)%,apoptosis rate:(35.91±1.80)%].CD437 at 10μmol/L inhibited proliferation,induced apoptosis and G0/G1 cell cycle arrest of human malignant melanoma A375 cells after treatment for 24 h[proliferation inhibiting rate:(58.6±2.38)%,apoptosis rate:(28.03±0.77)%].Celecoxib in combination with CD437 could significantly enhance the effects of inhibiting proliferation and inducing apoptosis of human malignant melanoma A375 cells 24 h after treatment compared with the drug alone[proliferation inhibiting rate:(68.92±1.72)%,apop-tosis rate:(42.09±1.05)%,both P<0.05]and decrease the proportion of the S phase in the cell cycle.Celecoxib could inhibit the growth of human malignant melanoma A375 cells by inducing apoptosis and G2/M cycle arrest.CD437 could inhibit the growth of human malignant melanoma A375 cells by inducing apoptosis and G0/G1 cycle arrest.Celecoxib exhibited additive effects with CD437 on retarding the growth and inducing apoptosis of human malignant melanoma A375 cells.Celecoxib in combination with CD437 may become an effective method for prevention and treatment of human melanoma.展开更多
基金supported by the National Natural Science Foundation of China(No.30371295).
文摘Objective： To investigate the effects of synthetic retinoid CD437 and acitretin on cell proliferation, apoptosis, cycle arrest and Bax/ Bcl-2 protein expression of melanoma A375 cell, Methods：MTT assay was used to determine the anti-proliferative effects of CD437 and acitretin on melanoma A375 cell, Flow cytometry was performed to investigate the influence of CD437 and acitretin on cell cycle and cell apoptosis. SABC immunocytochemistry was employed for detection of Bax/bcl-2 protein expressions. Results：10^-5 mol/L CD437 was more effective than acitretin in inhibiting proliferation and inducing apoptosis of A375 cell after 24 h treatment, growth inhibiting ratio and apoptosis ratio（58.6%vs43.25% and 28.03%vs17.13%, P 〈 0.05 respectively）. CD437 promoted G0/G1 arrest in melanoma A375 cell, however acitretin could not. CD437 and acitretin could up-regulate the expression of Bax protein and downregulate the expression of bcl-2 protein（P 〈 0.05）. Conclusion：CD437 is more effective than acitretin in inhibiting proliferation and inducing apoptosis and cycle arrest on A375 cell, CD437 may have more potentialities than acitretin for subsidiary treatment of melanoma. Mitochondrial apoptosis pathway is partially involved in two drugs inducing apoptosis on A375 cell.
基金supported by the National Natural Science Foundation of China(Grant No.30371295).
文摘This study aimed to investigate the effects of celecoxib,synthetic retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalenecarboxylicacid(CD437)and the combination of the two on cell proliferation,apoptosis,and cycle arrest of human malignant mela-noma A375 cells.3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazoliumbromide assay(MTT assay)was applied to determine the anti-proliferative effects of the drugs on human malignant melanoma A375 cells.Flow cytometry was performed to investigate the influence of the drugs on cell cycle and cell apoptosis.Both celecoxib and CD437 could inhibit the growth of human malignant melanoma A375 cells in a dose-dependent manner.Celecoxib at 80μmol/L inhibited proliferation,induced apoptosis and G2/M cell cycle arrest of human malignant melanoma A375 cells after treatment for 24 h[proliferation inhibiting rate:(50.2±2.51)%,apoptosis rate:(35.91±1.80)%].CD437 at 10μmol/L inhibited proliferation,induced apoptosis and G0/G1 cell cycle arrest of human malignant melanoma A375 cells after treatment for 24 h[proliferation inhibiting rate:(58.6±2.38)%,apoptosis rate:(28.03±0.77)%].Celecoxib in combination with CD437 could significantly enhance the effects of inhibiting proliferation and inducing apoptosis of human malignant melanoma A375 cells 24 h after treatment compared with the drug alone[proliferation inhibiting rate:(68.92±1.72)%,apop-tosis rate:(42.09±1.05)%,both P<0.05]and decrease the proportion of the S phase in the cell cycle.Celecoxib could inhibit the growth of human malignant melanoma A375 cells by inducing apoptosis and G2/M cycle arrest.CD437 could inhibit the growth of human malignant melanoma A375 cells by inducing apoptosis and G0/G1 cycle arrest.Celecoxib exhibited additive effects with CD437 on retarding the growth and inducing apoptosis of human malignant melanoma A375 cells.Celecoxib in combination with CD437 may become an effective method for prevention and treatment of human melanoma.
