食管鳞癌组织中CD44v4/5的表达及其与浸润转移的关系

用免疫组化ＬＳＡＢ法检测了４２例食管鳞癌中ＣＤ４４ｖ４／５的表达，结果发现：淋巴结转移组的阳性表达率为７６．１９％（１６／２１），而非转移组的阳性表达率为４２．８６（９／２１），两组间差异显著（Ｐ＜０．０５）。从Ⅰ级鳞癌到Ⅲ级鳞癌，其阳性率依次为６９．２３％（９／１３）、６４．７１％（１１／１７）和４１．６７％（５／１２），彼此间差异无显著性（Ｐ＞０．０５）。癌巢周边的癌细胞，肌间浸润的癌细胞，有核分裂的癌细胞和癌栓中的癌细胞及浸润脉管壁的癌细胞均呈强阳性表达。提示：ＣＤ４４ｖ４／５表达与食管鳞癌的浸润和淋巴道、血道转移有关。

食管鳞癌中CD44v4/5、MMP-10的表达与淋巴结转移的关系

目的研究食管鳞癌中CD44v4/5、MMP-10的表达与浸润转移的关系。方法用免疫组化S-P法检测了73例食管鳞癌中CD44v4/5、MMP-10的表达。结果CD44v4/5在淋巴结转移组的阳性表达率为64.6%(31/48),而在非转移组的阳性表达率为32.0%(8/25),两组之间差异显著(P<0.05)。MMP-10在淋巴结转移组的阳性表达率为79.2%(38/48),而在非转移组阳性表达率为40.0%(10/25),两组之间亦具有显著差异(P<0.05)。结论CD44v4/5和MMP-10的表达均与食管鳞癌浸润密切相关。

食管鳞癌发生过程中CD44v4/5的表达

用免疫组化ＬＳＡＢ法检测４２例食管鳞癌切除标本癌旁粘膜中ＣＤ４４ｖ４／５的表达。结果发现：癌旁正常上皮、单纯增生上皮、轻度不典型增生上皮、重度不典型增生上皮和原位癌中的阳性表达率分别为１１．１１％（２／１８）、１７．３９％（４／２３）、５６．２５％（９／１６）、５７．１４％（８／１４）和６９．２３％（９／１３），正常上皮、单纯增生上皮和轻、重度不典型增生上皮及原位癌之间差异显著（Ｐ＜０．０１）。结果提示：ＣＤ４４ｖ４／５表达与食管鳞癌的发生发展有关。

食管鳞癌中CD44v4/5、CD44v6的表达与浸润转移的关系

目的 探讨食管鳞癌中CD44v4/5、CD44v6的表达与浸润转移的关系。方法 用免疫组化LSAB法检测了 42例食管鳞癌中CD44v4/5、CD44v6的表达。结果 CD44v4/5在淋巴结转移组的阳性表达率为 76 19% (16/2 1) ,而在非转移组的阳性表达率为42 86% (9/2 1) ,两组之间差异显著 (P <0 0 5 )。CD44v6在淋巴结转移组的阳性表达率为 71 43 % (15 /2 1) ,而在非转移组阳性表达率为 3 8 0 9% (8/2 1) ,两组之间亦具有显著差异 (P <0 0 5 )。从Ⅰ级癌到Ⅲ级癌 ,CD44v4/5的阳性率依次为 69 2 3 % (9/13 )、64 71%(11/17)和 41 67% (5 /12 ) ,彼此间无显著差异 (P >0 0 5 )。CD44v6的阳性率依次为 76 92 % (10 /13 )、5 2 94% (9/17)、3 3 3 3 % (4 /12 ) ,各组之间无显著差异 (P >0 0 5 )。CD44v4/5和CD44v6的阳性率之间差异无显著性 ,也无相关关系。癌巢周围向间质浸润的癌细胞、肌间浸润的癌细胞 ,有核分裂的癌细胞和癌栓中的癌细胞及浸润血管壁的癌细胞CD44v4/5和CD44v6均呈强阳性表达。结论 CD44v4/5和CD44v6的表达均与食管鳞癌浸润转移有关。

Expression of human chorionic gonadotropin, CD44v6 and CD44v4/5 in esophageal squamous cell carcinoma

AIM: To study the relationship between the expression of human chorionic gonadotropin (HCG), CD44v6,CD44v4/5 and the infiltration, metastasis of esophageal squamous cell carcinoma. METHODS: By labeled streptavidin-biotin technique,the expressions of HCG, CD44v6, and CD44v4/5 in 42 patients with esophageal squamous cell carcinoma were examined.RESULTS: The positive rate of HCG expression in patients with lymph node metastasis was 85.71%(18/21), higher than that (57.14%, 12/21) in those without lymph node metastasis (P＜0.05). The positiverate of CD44v6 expression was 71.43% (15/21) in lymph node metastasis group, and 38.09% (8/21) in nonmetastasis group; there was a significant difference between the two groups (P＜0.05). The positive rateof CD44v4/5 expression was 76.19% (16/21) inlymph node metastasis group, and 42.86% (9/21) in non-metastasis group; there was also a significant difference between them (P＜0.05). From grade I to grade Ⅲ in differentiation, the positive rate of HCG expression was 84.62% (11/13), 70.59% (12/17) and 58.33% (7/12), respectively; there was no significant difference among them (P＞0.05). The positive rate of CD44v6 expression in grades Ⅰ -Ⅲ of cancer tissues was 76.92% (10/13), 52.94% (9/17), and 33.33%(4/12) respectively; there was no significant difference among them. The positive rate of CD44v4/5 expression in grades Ⅰ-Ⅲ of cancer tissues was 69.23% (9/13),64.71% (11/17), and 41.67% (5/12) respectively; there was no significant difference among the three groups. There was no correlation between the positive rates ofHCG and CD44v6, CD44v4/5 expression. Cancer cells in carcinomatous emboli and those infiltrating into vascular wall strongly expressed HCG, CD44v6, and CD44v4/5. CONCLUSION: Expression of HCG, CD44v6, and CD44v4/5 in esophageal squamous cell carcinoma is related to its infiltration and metastasis. HCG, CD44v6, and CD44v4/5 have different effects on the infiltration and metastasis of esophageal squamous cell carcinoma.