Expression of human chorionic gonadotropin, CD44v6 and CD44v4/5 in esophageal squamous cell carcinoma

AIM： To study the relationship between the expression of human chorionic gonadotropin （HCG）, CD44v6, CD44v4/5 and the infiltration, metastasis of esophageal squamous cell carcinoma. METHODS： By labeled streptavidin-biotin technique, the expressions of HCG, CD44v6, and CD44v4/5 in 42 patients with esophageal squamous cell carcinoma were examined. RESULTS： The positive rate of HCG expression in patients with lymph node metastasis was 85.71% （18/21）, higher than that （57.14%, 12/21） in those without lymph node metastasis （P〈0.05）. The positive rate of CD44v6 expression was 71.43% （15/21） in lymph node metastasis group, and 38.09% （8/21） in nonmetastasis group; there was a significant difference between the two groups （P〈0.05）. The positive rate of CD44v4/5 expression was 76.19% （16/21） in lymph node metastasis group, and 42.86% （9/21） in non-metastasis group; there was also a significant difference between them （P〈0.05）. From grade Ⅰ to grade Ⅲ in differentiation, the positive rate of HCG expression was 84.62% （11/13）, 70.59% （12/17） and 58.33% （7/12）, respectively, there was no significant difference among them （P〉0.05）. The positive rate of CD44v6 expression in grades Ⅰ-Ⅲ of cancer tissues was 76.92% （10/13）, 52.94% （9/17）, and 33.33% （4/12） respectively; there was no significant difference among them. The positive rate of CD44v4/5 expression in grades Ⅰ-Ⅲ of cancer tissues was 69.23% （9/13）, 64.71% （11/17）, and 41.67% （5/12） respectively; there was no significant difference among the three groups. There was no correlation between the positive rates of HCG and CD44v6, CD44v4/5 expression. Cancer cells in carcinomatous emboli and those infiltrating into vascular wall strongly expressed HCG, CD44v6, and CD44v4/5. CONCLUSION： Expression of HCG, CD44v6, and CD44v4/5 in esophageal squamous cell carcinoma is related to its infiltration and metastasis. HCG, CD44v6, and CD44v4/5 have different effects on the infiltration and metastasis of esophageal squamous cell carcinoma.

Relationship between expression of CD44v6 and nm23-H1 and tumor invasion and metastasis in hepatocellular carcinoma

AIM To detect the expression of CD44v6 mRNA and nm23-H1 mRNA in hepatocellular carcinoma (HCC) by in situ hybridization, and to evaluate the relationship between their expression and also relationship between their expressions and tumor invasion and metastasis.METHODS CD44v6 cDNA probe was synthesized with PCR technique and the nm23-H1 cRNA probe by in vitro transcription. The expression of CD44v6 mRNA and nm23-H1 mRNA was detected by in situ hybridization.RESULTS In group with high invasion and metastasis potential, the positive rates of CD44v6 mRNA and nm23-H1 mRNA were 80% (8/10) and 40% (4/10), in group with poor invasion and metastasis potential, they were 21.7% (5/23) and 91.3% (21/23). There was a positive correlation between the expression of CD44v6 mRNA and tumor invasion and metastasis potential in HCC (P＜0.01), and a reverse correlation between the expression of nm23-H1 mRNA and tumor invasion and metastasis potential (P＜0.01) and a reverse correlation in the expression between CD44v6 mRNA and nm23-H1 mRNA in HCC (P＜0.01).CONCLUSION Detection of CD44v6 mRNA and nm23-H1 mRNA may be useful for tumor invasion and metastasis in HCC.INTRODUCTIONCD44 is a cell surface transmembrane glycoprotein. As a kind of adhesive molecule, it participates in cell-cell and cell-matrix adhesion and interactions. Many studies revealed a correlation between high-level expression of CD44, especially CD44v and tumor invasion, metastasis and prognosis. The exon 6v containing isoforms may be an independent diagnostic parameter[1,2]. Some other studies, however, had different results[3,4]. Some researches showed a reverse correlation between the expression of nm23-H1 mRNA and tumor metastasis[5,6]. In order to evaluate the relationship between the expression of CD44v6 mRNA and nm23-H1 mRNA and tumor invasive and metastatic potential in HCC and to evaluate the relationship in the expression between CD44v6 mRNA and nm23-H1 mRNA, we detected their expression in HCC by in situ hybridization.

Expression of Bmi-1,P16,and CD44v6 in Uterine Cervical Carcinoma and Its Clinical Significance

Objective Bmi-1, a putative proto-oncogene, is a core member of the polycomb gene family, which is expressed in many human tumors. The p16 protein negatively regulated cell proliferation, whereas CD44v6 is associated with proliferation as an important protein. Additionally, CD44v6 is an important nuclear antigen closely correlated to tumor metastasis. Tlle present study aims to investigate the expression and significance of Bmi-1, p16, and CD44v6 in uterine cervical carcinoma （UCC）. Methods A total of 62 UCC, 30 cervical neoplasic, and 20 normal cervical mucosal tissues were used ill the current study. The expression of Bmi-1, p16, and CD44v6 in these tissues was determined using immunohistochemical assay. The relationships among the expression of these indices, the clinicopathologic features of UCC, and the survival rate of UCC patients were also discussed. The correlation between Bmi-1 protein expression and p16 or CD44v6 protein in UCC was analyzed. Results The expression of Bmi-l, p16, and CD44v6 was significantly high in cervical carcinoma compared with that in tlle cervical neoplasia and normal colorectal mucosa （P〈0.05）. The over-expression of Bmi-1 protein in UCC was apparently related to the distant metastasis （P〈0.01） and the tumor, nodes and metastasis-classification, i.e. the TNM staging, World Health Organization （P〈0.05）. Nevertheless, the positive expression of p16 protein in UCC was not significantly associated with the clinicopathologic features （P〉0.05）. The Kaplan-Meier survival analysis showed that the over-expression of Bmi-1 significantly decreased the survival rate of UCC patients （P〈0.05）. A strong correlation indicated that there was statistical significance between the expression of Bmi-1 and CD44V6 proteins in UCC （r=0.419, P=0.001）. Conclusions The over-expression of Bmi-1 and CD44v6 protein closely correlate to the tumorigenesis, metastasis, and prognosis of UCC. Bmi-I and CD44v6 may be used to predict the prognosis of cervical carcinoma. Bmi-1 may indirectly regulate the expression of CD44v6 in UCC patients. The positive expression of p16 protein is possibly associated with the tumorigenesis, but not with the metastasis or prognosis of UCC.

Relationship between the Expression of CD44v6 and Development,Progress, Invasion and Metastasis of Laryngeal Carcinoma

The expression of CD44v6 and its relationship with the development, progress, invasion and metastasis of laryngeal carcinoma was investigated. The expression and content of CD44v6 mRNA in tissuess were detected by both RT-PCR and FCM which were respectively extracted from normal laryngeal mucosa, leukoplakia of larynx, laryngeal papilloma, polyp of vocal cord, tissues of laryngeal carcinoma, metastatic and nonmetastatic lymph nodes of neck, and tissues close to carcinoma. The outcome of RT-PCR indicated that the expression rate of CD44v6 mRNA involved in tissues of laryngeal carcinoma and metastatic lymph nodes of neck was the highest (90 %-100 %) compared with that of leukoplakia of larynx, laryngeal papilloma, tissues close to carcinoma by 0.5 cm (55.56 %-60.00 %) and that of normal laryngeal mucosa, polyp of vocal cord, nonmetastatic lymph nodes and tissues close to carcinoma by 1.0 cm was the lowest ( 13.33 %-20 %). The result from FCM was highly consistent with that from RT-PCR. It was suggested that CD44v6 was closely related with the development, progress, invasion and metastasis of laryngeal carcinoma. The outcome from the tissues close to carcinoma by different distance could do help to the determination of incisal edge in surgery abstractly.

AN IMMUNOHISTOCHEMICAL STUDY ON EXPRESSION OF CD44v6 PROTEIN IN HUMAN GALLBLADDER CARCINOMA

Objective To investigate the clinical significances of CD44v6 protein expression in human gallbladder carcinoma(GBC).Methods The immunohistochemical technique was used to detect the CD44v6 protein expression in 40 cases of GBC,12 cases of chronic cholecystitis,6 cases of adenoma and 4 cases of adenomyomatous hyperplasia.Results The positive rate of CD44v6 expression in gallbladder carcinoma and benign lesions was 72.5% and 0%,respectively( P <0.01),and it also correlated with the lymph node metastasis,pathologic differentiation and clinic staging,but there was no correlation among pathological types.Conclusion Detecting CD44v6 expression might be severed as an objective indicator for differential diagnosis,lymph node metastatic potency,tumor progress and prognosis in gallbladder carcinoma.

TSH抑制疗法对分化型甲状腺癌患者术后血清Tg、VEGF、TSGF、CD44V6、sIL-2R及T淋巴细胞亚群水平的影响

目的:探讨促甲状腺激素(thyroid stimulating hormone,TSH)抑制疗法对分化型甲状腺癌(differentiated thyroid carcinoma,DTC)患者术后血清甲状腺球蛋白(thyroglobulin,Tg)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、肿瘤特异性生长因子(tumors pecific growth factor,TSGF)、白细胞分化抗原44变异型6(CD44V6)、可溶性白细胞介素-2受体(soluble interleukin-2receptor,sIL-2R)及T淋巴细胞亚群水平的影响。方法:选择2014年1月~2017年1月我院收治的接受甲状腺全切手术治疗的100例DTC患者,随机分为对照组和实验组,各50例。对照组患者常规给予甲状腺素替代治疗,实验组患者给予TSH抑制疗法(口服左甲状腺素钠片,控制血清TSH水平低于0.1mU/L),两组患者均给予治疗1个月。比较两组患者治疗前后血清Tg、VEGF、TSGF、CD44V6、sIL-2R水平及外周血CD3^+、CD4^+、CD8^+水平。结果:两组治疗前的血清Tg、VEGF、TSGF、CD44V6、sIL-2R水平比较,均无显著性差异(P>0.05);两组治疗后的血清Tg、VEGF、TSGF、CD44V6、sIL-2R水平相比治疗前均较低,且实验组治疗后血清Tg、VEGF、TSGF、CD44V6、sIL-2R水平变化均显著优于对照组(P<0.05)。两组治疗前的外周血CD3^+、CD4^+、CD8^+水平比较,均无显著性差异(P>0.05);两组治疗后的外周血CD3^+、CD4^+水平相比治疗前均较高、CD8^+水平相比治疗前均较低,且实验组治疗后血外周血CD3^+、CD4^+、CD8^+水平变化均优于对照组,具有显著性差异(P<0.05)。结论:DTC行甲状腺全切手术治疗后接受TSH抑制疗法能够有效降低血清Tg、VEGF、TSGF、CD44V6、sIL-2R水平,改善细胞免疫功能,值得在临床上推广应用。

CD44v6在宫颈癌组织中的表达及其临床意义

目的:探讨宫颈癌组织中CD 44 v 6的表达与其浸润转移的关系。方法:用免疫组织化学S-P法,检测48例宫颈癌、33例宫颈上皮内瘤样病变及15例正常宫颈上皮组织中的CD 44 v6的表达,分析相关的临床病理因素。同时对术前留取21例宫颈癌活组织也进行了CD 44 v 6的检测。结果:宫颈浸润癌中CD 44 v 6的阳性表达(68.75%)显著高于正常宫颈(13.33%)及宫颈上皮内瘤样病变(45.45%)(P<0.05)。CD 44 v 6的表达在有淋巴转移组中(94.74%)明显高于无淋巴结转移组(51.72%)。随着病理分级的升高,CD44v6的表达增强,其Ⅰ级、Ⅱ级、Ⅲ级的CD 44 v 6阳性率分别为40.00%、61.90%、94.12%(P<0.05)。术前组中CD 44 v 6阳性表达者淋巴结转移率(64.29%)高于CD 44 v 6阴性组(14.29%),但无统计学差异(P>0.05)。CD 44 v 6的表达与组织学类型、临床分期等因素无关(P>0.05)。结论:CD 44 v 6的表达与宫颈癌的进展转移密切相关,检测CD 44 v 6的表达有助于预测浸润转移,并可能为宫颈癌的治疗提供新的靶点。

CD44v6和PTEN基因蛋白在子宫内膜癌中的表达及意义

目的:探讨子宫内膜癌(endom etrial carcinom a,E C)组织中C D44v6和PTE N蛋白表达及临床意义。方法:采用免疫组织化学方法检测50例E C组织C D44v6和PTE N蛋白表达。结果:在E C中C D44v6和PTE N表达阳性率分别为70.0%和38.0%,均与E C的组织学分级、临床分期和转移相关(P<0.05)。结论:C D44v6和PTE N表达对判断E C的恶性程度、预测肿瘤侵袭转移和评估预后的参考指标。

Expression and significance of CD44s, CD44v6, and nm23 mRNA in human cancer

AIM: To investigate the relationship between the expression levels of nm23 mRNA, CD44s, and CD44v6,and oncogenesis, development and metastasis of human gastric adenocarcinoma, colorectal adenocarcinoma,intraductal carcinoma of breast, and lung cancer.METHODS: Using tissue microarray by immuhistochemical (IHC) staining and in situ hybri-dization (ISH), we examined the expression levels of nm23mRNA, CD44s, and CD44v6 in 62 specimens of human gastric adenocarcinoma and 62 specimens of colorectal adenocarcinoma; the expression of CD44s and CD44v6in 120 specimens of intraductal carcinoma of breast and 20 specimens of normal breast tissue; the expression of nm23 mRNA in 72 specimens of human lung cancer and 23 specimens of normal tissue adjacent to cancer.RESULTS: The expression of nm23 mRNA in the tissues of gastric and colorectal adenocarcinoma was not significantly different from that in the normal tissues adjacent to cancer (P＞0.05), and was not associated with the invasion of tumor and the pathology grade of adenocarcinoma (P＞0.05). However, the expression of nm23 mRNA was correlated negatively to the lymph node metastasis of gastric and colorectal adenocarcinoma (r = -0.49, P＜0.01; r = -4.93, P＜0.01). The expression of CD44s in the tissues of gastric and colorectal adenocarcinoma was significantly different from that in the normal tissues adjacent to cancer (P＜0.05;P＜0.01). CD44v6 was expressed in the tissues of gastric and colorectal adenocarcinoma only, the expression of CD44v6 was significantly associated with the lymph node metastasis, invasion and pathological grade of the tumor (r = 0.47, P＜0.01; r = 5.04, P＜0.01). CD44sand CD44v6 were expressed in intraductal carcinoma of breast, the expression of CD44s and CD44v6 was significantly associated with lymph node metastases and invasion (P＜0.01). However, neither of them was expressed in the normal breast tissue. In addition, the expression of CD44v6 was closely related to the degree of cell differentiation of intraductal carcinoma of breast (x2= 5.68, P＜0.05). The expressional level of nm23mRNA was closely related to the degree of cell differentiation (P＜0.05) and lymph node metastasis (P＜0.01), but the expression of nm23 gene was not related to sex, age, and type of histological classification (P＞0.05).CONCLUSION: Patients with overexpression of CD44s and CD44v6 and low expression of nm23 mRNA have a higher lymph node metastatic rate and invasion. In addition, overexpression of CD44v6 is closely related to the degree of cell differentiation. Detection of the three genes is able to provide a reliable index to evaluate the invasion and metastasis of tumor cells.

Neoadjuvant chemotherapy with pingyangmycin can inhibit the amplification and metastasis of laryngeal squamous cell carcinoma

Objective:To evaluate the short-term effects of neoadjuvant chemotherapy with pingyangmycin(PYM)in the treat-ment of laryngeal squamous cell carcinoma.Methods:24 cases of laryngeal squamous cell carcinoma were treated with PYM before the operation,and the surgeries were undergone within one week after neoadjuvant chemotherapy.PCNA,p53,Bcl-2 and CD44v6 were detected in the specimens of tumor,retreated tumor and normal tissue using immunohistochemical methods.Results:Apoptosis could be detected more often in specimens with tumor and retreated tumor after chemotherapy than that before.The expression of PCNA,p53,Bcl-2 and CD44v6 in tumor tissue after neoadjuvant chemotherapy with PYM was weaker than that before the chemotherapy.There was significant difference in the positive ratio of PCNA,p53,Bcl-2 and CD44v6 be-tween retreated tumor and tumor.Conclusion:After neoadjuvant chemotherapy with PYM,a large number of tumor cells died.The amplification and metastasis of tumor were suppressed by neoadjuvant chemotherapy with PYM.

The study on quantitative expression of CD44v6mRNA by real-time RT-PCR with the micro-metastases of gastric cancer

Objective： To study the expression of CD44 correlation and the ability of metastasis of tumor cells in gastric carcinoma, and to find the correlation of the quantitative of CD44V6mRNA and the histology expression of CD44v6 in tumors with the clinic-pathologic features, and to make the quantitative expression of CD44v6mRNA. Methods： Twenty patients with gastric carcinoma, 4 patients with gastritis, and 10 apparently healthy controls were recruited. Blood samples were obtained before surgery. 10 days after surgery, the blood samples were obtained again. Serum CIM4v6mRNA in all cases was measured by real-time quantitative PCR. Results： Serum CIM4V6mRNA was detectable in 20 of 20（ 100% ） gastric carcinoma cases, The expression level ranged from 4.9×10^2 copies/μg RNA to 3.2 ×10^5 copies/μg RNA, the average levels of peripheral blood was 3.9 ×10^4 copies/μg RNA, The expression level of pedpheral blood of gastric cancer after curative operation ranged from 5.5×10^0 copies/μg RNA to 7.6 ×10^3 copies/μg RNA. After curative operation the expression level was decreased markedly. Conclusion： Serurn CIM4v6mRNA is expressed in the peripheral blood of gastric carcinoma patients. The expression level of CD44V6mRNA is obviously decreased after curative operation. An elevated level of CD44v6mRNA may serve as an indicator of lymph node metastasis （especially early metastasis） and bad prognosis in patients with gastric carcinoma.

消痰散结方对MKN-45人胃癌细胞黏附分子CD_(44)V_6表达的影响

目的 :观察消痰散结方对体外培养的 MKN- 45人胃癌细胞黏附分子 CD44 V6表达的影响 ,初步探讨了消痰散结方抑制胃癌细胞转移的作用环节。方法 :制备消痰散结药物血清 ,体外培养 MKN- 45人胃癌细胞 ,拟消痰散结方药物血清干预培养细胞 ,采用免疫组化 ABC法检测胃癌细胞中 CD44 V6的表达。结果 :中药组胃癌细胞中CD44 V6表达水平明显低于空白对照组。结论 :消痰散结方抑制胃癌细胞转移的环节可能和影响黏附分子的表达 。

乳腺癌的钼靶X线征象与CD_(44)V_6蛋白表达的关系

目的 :探讨乳腺癌钼靶X线征象与CD44V6蛋白表达的关系 ,旨在为乳腺癌的早期诊断、预后提供依据。材料和方法 :术后病理切片利用免疫组化技术测定 5 4例乳腺癌和 2 6例乳腺良性病变组织中CD44V6蛋白的表达 ,比较它们在乳腺癌和乳腺良性病变中的表达水平 ,分析其表达与乳腺癌钼靶X线征象的关系。结果 :乳腺癌组织中CD44V6蛋白的表达高于乳腺良性病变 ,但不同组织类型的乳腺癌其表达无显著性差异。乳腺癌中CD44V6的表达 ,肿块边缘有“毛刺征”组高于无“毛刺征”组 ,静脉增粗组高于正常组。但其表达与乳腺癌的簇样钙化、皮肤增厚、乳头收缩内陷以及肿块大小无关。CD44V6的表达与手术病理诊断的腋窝淋巴结转移有明显的关系 ,与钼靶X线腋窝的淋巴结显示无关。结论 :乳腺癌的钼靶X线一些重要征象与CD44V6的表达有密切的关系 。

卵巢上皮性肿瘤CD_(44)V_6的表达及其临床意义的研究

目的 :探讨 CD44 V6 表达与恶性卵巢上皮性肿瘤发生、发展及预后的关系。方法 :采用免疫组化 S- P方法 ,检测 2 0例良性、10例交界性及 5 5例恶性卵巢上皮性肿瘤组织 CD44 V6 的表达 ,并分析其与临床病理指标间的关系。结果 :(1) CD44 V6 表达阳性率及强度随良性、交界性、恶性卵巢上皮性肿瘤的进展而逐渐增加 ,差异有显著性 (P=0 .0 0 1,P强 =0 .0 13) ;在良性卵巢上皮性肿瘤中表达阳性率及强度均低于卵巢上皮性癌 (P=0 .0 0 0 ,P强 =0 .0 0 8)。(2 ) CD44 V6 的表达阳性率与卵巢癌的临床分期、淋巴结转移及腹水均无关 (P=0 .2 44 ,0 .0 72 ,0 .0 5 1) ;而表达强度与卵巢癌的临床分期 ,淋巴结转移及腹水有关 (P强 =0 .0 0 4,0 .0 0 1,0 .0 2 5 ) ;表达阳性率及强度与病理类型、组织分化均无关 (P=0 .134,0 .2 31;P强 =0 .186 ,1.0 0 0 )。 (3) CD44 V6 的阳性表达与卵巢癌的预后有关 (P=0 .0 42 ) ,表达越强 ,预后越差。结论 :CD44 V6 表达与卵巢癌的发生及转移密切相关。检测 CD44 V6 的表达有助于预测卵巢癌的进展程度、淋巴结转移趋势以及估计预后。

结肠癌nm23-H1和CD_(44)V_6表达及其与淋巴结转移的关系

目的 :探讨nm2 3 H1和CD4 4 V6在结肠癌发生、发展中的作用及其相互关系。方法 :采用免疫组化S P方法检测 15 2例结肠癌nm2 3 H1和CD4 4 V6的表达。结果 :15 2例结肠癌nm2 3 H1阳性率与淋巴结转移 (P <0 0 5 )、癌组织分化 (P <0 0 1)关系密切 ;CD4 4 V6表达与Dukes分期 (P <0 0 1)、肿瘤浸润深度 (P <0 0 1)、癌组织分化 (P <0 0 5 )及淋巴结转移 (P <0 0 1)密切相关 ;nm2 3 H1和CD4 4 V6表达 ,两者呈负相关 (P <0 0 1) ,CD4 4 V6阳性nm2 3 H1阴性表达的患者淋巴结转移率高于CD4 4 V6阴性nm2 3 H1阳性表达者 (P <0 0 5 )。结论 :nm2 3 H1和CD4 4 V6在结肠癌中的表达与其发生发展密切相关 ,尤其CD4 4 V6和nm2 3 H1表达在结肠癌转移中起协调作用 。

nm23和CD_(44)V_6在胃癌中的表达及其意义

目的 :研究nm2 3和CD44V6 在胃癌组织中的表达及临床意义。方法 :应用免疫组化技术对 84例胃癌组织进行表达检测 ,结合病理学指标进行分析。结果 :nm2 3和CD44V6 在 84例胃癌中的阳性表达率分别是 4 8 8%、5 2 4 %。nm2 3与胃癌的淋巴结转移密切相关 ,在淋巴结转移阳性组的阳性表达率为 32 7% ,显著低于淋巴结转移阴性组的阳性表达率 79 3% (P <0 .0 5 )。CD44V6 与胃部的浸润深度和淋巴结转移密切相关 ,在进展期胃癌和淋巴结转移阳性组的表达率分别为 5 8 9%和 70 9% ,显著高于早期胃癌和淋巴结转移阴性组的阳性表达率 9 1%和 17 2 % (P值分别为 <0 .0 5 ,<0 .0 1)。结论 :nm 2 3和CD44V6 在胃癌的发生、发展中均起着重要的调控作用。nm2 3的缺失表达对胃癌的淋巴结转移起重要作用 ,CD44V6 的高表达对胃癌的浸润、转移起重要作用。对分析胃癌的生物学行为和预后估计有重要意义。

转移相关基因CD_(44)V_6表达与乳癌转移和预后关系的研究

目的 :探讨 CD4 4V6表达与乳癌转移和预后之间的关系。方法 :用免疫组化染色方法和微波抗原修复技术 ,检测 78例原发性乳癌组织中 CD4 4V6蛋白的表达 ,分析各临床病理因素和 CD4 4V6表达与乳癌术后 5年生存率的关系。结果 :CD4 4V6的阳性表达率为 55.1% ( 4 3/ 78) ,腋淋巴结阳性乳癌中的表达率为 72 .7% ( 32 / 4 4 ) ,明显高于阴性乳癌中 CD4 4V6表达率 32 .4 % ( 11/ 34 ) ,P<0 .0 1。CD4 4V6阳性组乳癌患者的预后明显较阴性组差 ( P<0 .0 5)。结论 :CD4 4V6表达与乳癌转移和预后密切相关 ;CD4 4V6、腋淋巴结状况、肿瘤大小、组织学分级和 TNM分期是影响乳癌术后 5年生存率的重要因素。

卵巢肿瘤中CD_(44)V_6与PCNA的表达及其意义

目的 :探讨卵巢良恶性肿瘤中 CD4 4V6 与 PCNA的表达及其临床意义。方法 :应用免疫组化 SP法对 42例恶性卵巢肿瘤、 2 4例良性卵巢肿瘤和 10例正常卵巢组织中 CD4 4V6 与 PCNA的表达进行检测。结果 :CD4 4V6 与 PCNA的表达特点相似 ,两者在恶性卵巢肿瘤中的表达均高于在良性卵巢肿瘤及正常卵巢中的表达 ;在发生转移的恶性卵巢肿瘤中的表达均高于未发生转移的恶性卵巢肿瘤中的表达。结论 :CD4 4V6 与 PCNA在恶性卵巢肿瘤的发生、发展及浸润、转移中起重要作用 。

结直肠癌中CD_(44) V_6 PCNA的表达及其与浸润转移的关系

目的 探讨CD44V6和PCNA在结直肠癌中的表达及其与肿瘤浸润转移的关系。方法 应用免疫组织化学S P法检测 87例结直肠癌组织中CD44V6和PCNA的表达情况。结果 87例结直肠癌组织中CD44V6蛋白的表达阳性率为 5 8.6 % ,PCNALI为 5 6 .5 1± 18.82。CD44V6阳性表达率和PCNALI在侵及浆膜层者明显高于侵及肌层者 ,淋巴结转移阳性组高于淋巴结转移阴性组 ,DukesC、D期明显高于A、B期 ,差异均有显著性 (P <0 .0 5 )。PCNA高LI者CD44V6阳性率明显高于PCNA低LI者 ,有显著性差异 (P <0 .0 5 )。结论 CD44V6和PCNA在结直肠癌浸润转移过程中发挥重要作用 ,可作为反映结直肠癌恶性程度的生物学标记物。

口腔粘膜鳞癌发生发展过程中CD_(44)V_6表达的研究

目的 探讨 CD44 V6作为口腔鳞癌发生发展预测指标的可行性。方法 采用免疫组化 SP染色技术 ,观察CD44 V6在口腔正常粘膜、乳头状瘤、上皮异常增生及鳞状细胞癌中的表达情况。结果 乳头状瘤的 CD44 V6表达与正常口腔粘膜组织相似 ;上皮异常增生及鳞癌 CD44 V6呈明显下调表达 ,其趋势与上皮异常增生的程度及鳞癌分级相关 (P<0 .0 5)。结论 CD44 V6的下调表达是口腔粘膜鳞癌发生发展的早期变化 ,可作为鉴测口腔粘膜癌前病变的分子生物学指标。