Objective To detection the expression of membrane associated complement regulatory proteins(CRP) CD55 and CD59 on the major peripheral blood cells(erythrocytes and neutrophils) in patients with paroxysmal nocturnal he...Objective To detection the expression of membrane associated complement regulatory proteins(CRP) CD55 and CD59 on the major peripheral blood cells(erythrocytes and neutrophils) in patients with paroxysmal nocturnal hemoglobinuria(PNH),aplastic anemia,microcytic hypochromic anemia,macrocytic anemia,oncoma of hematopoietic system with anemia and autoimmune hemolytic anemia, and analysis its clinical implications.Methods Using fluorchrome (PE and FITC) labeled anti CD55 and anti CD59 monoclonal antibodies, and analyzing with flow cytometry.Results All the ratios of CD55 - and CD59 - cells in 35 normal peoples are less than 5%,but in excess of 40% in the 5 PNH patients.The results of Some patients with aplastic anemia are more than 5%, but less than 15%. About 50% patientswith microcytic hypochromic anemia having CD55 - and CD59 - erythrocytes aremore than 5%(less than 15%),but their neutrophils are normal.The results of patients with macrocytic anemia; oncoma of hematopoietic system with anemia and autoimmune hemolytic anemia are no more than 5%.Conclusion Detection of CD55 - and CD59 - erythrocytes and neutrophils in the peripheral blood is avaluable and sensitive test in diagnosis and differential diagnosis of PNH, aplastic anemia and microcytic hypochromic anemia, and it can be used to evaluate the curative effect of PNH.展开更多
Objectives: To explore the mechanism of development and aggressiveness in gastric carcinomas by investigating the expression and role of CD97 and its cellular ligand CD55 in gastric carcinomas. Methods: Tumor and corr...Objectives: To explore the mechanism of development and aggressiveness in gastric carcinomas by investigating the expression and role of CD97 and its cellular ligand CD55 in gastric carcinomas. Methods: Tumor and corresponding normal mucosal tissue, collected from 39 gastric carcinoma patients, were examined by immunohistochemistry and RT-PCR for the expression of CD97 and CD55. Results: CD97stalk was strongly stained on scattered tumor cells or small tumor cell clusters at the invasion front of gastric carcinomas. The expression of CD97stalk was frequently observed in tumors of stage I and T1 gastric carcinoma patients. The expression of CD97stalk between Stage I and Stage II, III, IV specimens showed significant difference (P<0.05), between T1 and T2, T3, T4 specimens also showed significant difference (P<0.05). Specimens with tumor invasion depth limited in mucosa of T1 specimens showed higher positive CD55 expression than specimens with the same tumor invasion depth in T2, T3, T4 specimens, the expression of CD55 between T1 and T2, T3, T4 specimens was significantly different (P<0.05). There was strong correlation between the distribution patterns of CD97stalk and CD55 on tumor tissues (r=0.73, P<0.05). Signet ring cell carcinomas frequently contained strong CD97stalk and CD55-staining. Conclusions: Our results suggest that CD97stalk is probably involved in the growth, invasion and aggressiveness of gastric carcinomas by binding its cellular ligand CD55. CD97stalk and CD55 could be useful as molecular markers for prognosis and therapy of gastric carcinoma patients.展开更多
文摘Objective To detection the expression of membrane associated complement regulatory proteins(CRP) CD55 and CD59 on the major peripheral blood cells(erythrocytes and neutrophils) in patients with paroxysmal nocturnal hemoglobinuria(PNH),aplastic anemia,microcytic hypochromic anemia,macrocytic anemia,oncoma of hematopoietic system with anemia and autoimmune hemolytic anemia, and analysis its clinical implications.Methods Using fluorchrome (PE and FITC) labeled anti CD55 and anti CD59 monoclonal antibodies, and analyzing with flow cytometry.Results All the ratios of CD55 - and CD59 - cells in 35 normal peoples are less than 5%,but in excess of 40% in the 5 PNH patients.The results of Some patients with aplastic anemia are more than 5%, but less than 15%. About 50% patientswith microcytic hypochromic anemia having CD55 - and CD59 - erythrocytes aremore than 5%(less than 15%),but their neutrophils are normal.The results of patients with macrocytic anemia; oncoma of hematopoietic system with anemia and autoimmune hemolytic anemia are no more than 5%.Conclusion Detection of CD55 - and CD59 - erythrocytes and neutrophils in the peripheral blood is avaluable and sensitive test in diagnosis and differential diagnosis of PNH, aplastic anemia and microcytic hypochromic anemia, and it can be used to evaluate the curative effect of PNH.
基金Project (No. 2004C34010) supported by the Science and Technology Bureau of Zhejiang Province, China
文摘Objectives: To explore the mechanism of development and aggressiveness in gastric carcinomas by investigating the expression and role of CD97 and its cellular ligand CD55 in gastric carcinomas. Methods: Tumor and corresponding normal mucosal tissue, collected from 39 gastric carcinoma patients, were examined by immunohistochemistry and RT-PCR for the expression of CD97 and CD55. Results: CD97stalk was strongly stained on scattered tumor cells or small tumor cell clusters at the invasion front of gastric carcinomas. The expression of CD97stalk was frequently observed in tumors of stage I and T1 gastric carcinoma patients. The expression of CD97stalk between Stage I and Stage II, III, IV specimens showed significant difference (P<0.05), between T1 and T2, T3, T4 specimens also showed significant difference (P<0.05). Specimens with tumor invasion depth limited in mucosa of T1 specimens showed higher positive CD55 expression than specimens with the same tumor invasion depth in T2, T3, T4 specimens, the expression of CD55 between T1 and T2, T3, T4 specimens was significantly different (P<0.05). There was strong correlation between the distribution patterns of CD97stalk and CD55 on tumor tissues (r=0.73, P<0.05). Signet ring cell carcinomas frequently contained strong CD97stalk and CD55-staining. Conclusions: Our results suggest that CD97stalk is probably involved in the growth, invasion and aggressiveness of gastric carcinomas by binding its cellular ligand CD55. CD97stalk and CD55 could be useful as molecular markers for prognosis and therapy of gastric carcinoma patients.
