Donor-derived CD 19 CAR-T Cells versus Chemotherapy Plus Donor Lymphocyte Infusion for Treatment of Recurrent CD 19-positive B-ALL after Allogeneic Hematopoietic Stem Cell Transplantation

Objective:This study aimed to compare the efficacy of anti-CD19 chimeric antigen receptor T cells(CAR-T cells)versus chemotherapy plus donor lymphocyte infusion(chemo-DLI)for treating relapsed CD 19-positive B-cell acute lymphoblastic leukemia(B-ALL)after allogeneic hematopoietic stem cell transplantation(allo-HSCT).Methods:Clinical data of 43 patients with B-ALL who relapsed after allo-HSCT were retrospectively analyzed.Twenty-two patients were treated with CAR-T cells(CAR-T group),and 21 with chemotherapy plus DLI(chemo-DLI group).The complete remission(CR)and minimal residual disease(MRD)-negative CR rates,leukemia-free survival(LFS)rate,overall survival(OS)rate,and incidence of acute graft-versus-host disease(aGVHD),cytokine release syndrome(CRS)and immune effector cell-associated neurotoxicity syndrome(ICANS)were compared between the two groups.Results:The CR and MRD-negative CR rates in the CAR-T group(77.3%and 61.5%)were significantly higher than those in the chemo-DLI group(38.1%and 23.8%)(P=0.008 and P=0.003).The 1-and 2-year LFS rates in the CAR-T group were superior to those in the chemo-DLI group:54.5%and 50.0%vs.9.5%and 4.8%(P=0.0001 and P=0.00004).The 1-and 2-year OS rates in the CAR-T versus chemo-DLI group were 59.1%and 54.5%vs.19%and 9.5%(P=0.011 and P=0.003).Six patients(28.6%)with grade 2-4 aGVHD were identified in the chemo-DLI group.Two patients(9.1%)in the CAR-T group developed grade 1-2 aGVHD.Nineteen patients(86.4%)developed CRS in the CAR-T group,comprising grade 1-2 CRS in 13 patients(59.1%)and grade 3 CRS in 6 patients(27.3%).Two patients(9.1%)developed grade 1-2 ICANS.Conclusion:Donor-derived anti-CD19 CAR-T-cell therapy may be better,safer,and more effective than chemo-DLI for B-ALL patients who relapse after allo-HSCT.

Effect of Mg^(2+)level on the functions of CD8^(+)T lymphocytes and NK cells in patients with COVID-19

Objective:To investigate the changes of Mg^(2+) levels in serum and peripheral blood mononuclear cells(PBMCs)of patients with COVID-19 and its effects on the functions of CD8^(+)T lymphocytes and NK cells.Methods:A total of 165 COVID-19 patients hospitalized in Ezhou Central Hospital from January 20 to February 20,2020 were divided into mild/common group(98 cases)and severe/critical group(67 cases).At the same time,34 healthy persons were selected as the control group.Peripheral blood was collected and PBMCs were isolated,the level of Mg^(2+) in serum and PBMCs was detected.The subsets of CD8^(+)T lymphocytes and NK cell and the expression levels of their surface inhibitory molecular PD-1 and activator molecular NKG2D were detected by flow cytometry.The correlation between Mg^(2+) concentration and the expression levels of PD-1 and NKG2D was also analyzed.Results:Compared with the control group,the concentration of Mg^(2+) in serum and PBMCs,the counts of CD8^(+)T lymphocytes and NK cell in patients with mild/common and severe/critical groups were significantly reduced(P<0.05),while the expression level of surface inhibitory molecular PD-1 were significantly increased(P<0.05),while the expression level of the activation molecule NKG2D were significantly decreased(P<0.05).However,the changes of the above indicators in patients with severe/critical group were greater than those in the mild/common group(P<0.05).In addition,the Mg^(2+) concentration in COVID-19 patients was negatively correlated with the expression level of PD-1 on CD8^(+)T lymphocytes and NK cells(P<0.05),and positively correlated with the expression levels of NKG2D(P<0.05).Conclusion:The concentration of Mg^(2+) in the serum and PBMCs of COVID-19 patients is significantly reduced,which may cause the function of CD8^(+)T lymphocytes and NK cells to be inhibited.

外周血CD8^(+)T淋巴细胞亚群检测对过敏性鼻炎临床疗效的评估价值

目的探讨外周血CD8^(+)T淋巴细胞亚群检测对过敏性鼻炎(AR)临床疗效的评估价值。方法前瞻性选取2021年3月~2022年2月温州市中西医结合医院耳鼻咽喉科门诊治疗的120例AR患者和40例健康志愿者,分别设为研究组和对照组,比较两组外周血CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞、CD8^(+)CD28-T细胞比率;研究组接受鼻用激素联合口服抗组胺药物治疗方案,根据治疗效果分为有效组(n=107)和无效组(n=13),比较有效组和无效组治疗前CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞、CD8^(+)CD28-T细胞比率;Pearson相关性分析法分析外周血CD8^(+)T淋巴细胞亚群水平与鼻部症状总评分(totalnose symptomscore,TNSS)的相关性;以受试者工作特征(ROC)曲线分析治疗前CD8^(+)T淋巴细胞亚群对AR临床疗效的预测价值。结果研究组治疗前外周血CD8^(+)CD28^(+)T细胞比率高于对照组,CD8^(+)CD28-T细胞比率低于对照组(P<0.05)。研究组总有效率为89.17%;有效组治疗前CD8^(+)CD28^(+)T细胞比率低于无效组,CD8^(+)CD28-T细胞比率高于无效组(P<0.05);Pearson相关性分析显示,CD8^(+)CD28^(+)T细胞比率与TNSS评分呈正相关(r=0.324,P=0.006),CD8^(+)CD28-T细胞比率与TNSS评分呈负相关(r=-0.541,P=0.000)。ROC曲线结果显示,CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞、CD8^(+)CD28-T细胞比率评估AR治疗无效的曲线下面积(AUC)(95%CI)分别为0.647(0.555~0.732)、0.683(0.592~0.765)、0.911(0.845~0.955),其中CD8^(+)CD28-T细胞的评估效能优于CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞比率(P<0.05)。结论AR患者外周血CD8^(+)CD28^(+)T细胞比率升高,CD8^(+)CD28-T细胞比率降低,CD8^(+)T细胞比率相对稳定,治疗前CD8^(+)CD28-T细胞比率可作为预测AR患者的临床疗效的参考指标。

Role of CD4+ and CD8+ T Lymphocyte in the Onset of Stroke in People Living with HIV in Pointe-Noire

Objective: To determine the role of CD4+ and CD8+ T lymphocytes in the onset of stroke in people living with HIV. Methodology: This was a descriptive, cross-sectional study from January to July 2019, in the neurology department of loandjili general hospital, including any patient hospitalized for a first episode of stroke confirmed by brain scan. The study variables were: age, sex, CRP value, serum T cell CD4+, CD8+. The statistical analysis was carried out using the EPI info 7 software. Results: Twenty stroke patients were included. The relative frequency of HIV was 20%. The risk factors were potentiated by immunosuppression of CD4+ T cells. Sixty percent (60%) of the patients had a CD4+ count < 200/mm3 and the mean CD4+ count was ±191/mm3. Stroke was the predominant mechanism of injury with a frequency of 70%, the only injury mechanism of stroke in patients with CD8+ T cell count > 800/mm3 (p = 0.04). Conclusion: Risk factors are potentiated by TCD4+ lymphocyte immunosupression, also CD8+ lymphocytes of immune system activation marker are a cardiovascular risk factor for living people with HIV.

Th9细胞对非小细胞肺癌患者CD8^+T细胞抗肿瘤活性的调控作用

目的:观察并分析Th9细胞及其分泌的白细胞介素-9(IL-9)对非小细胞肺癌(NSCLC)患者CD8^+T细胞抗肿瘤活性的影响。方法:选择32例NSCLC患者(肺癌组)和11例健康对照者(对照组)。分离肺癌组血浆、外周血单个核细胞(PBMCs)、肿瘤部位和非肿瘤部位的支气管肺泡灌洗液(BALF)及对照组血浆和PBMCs,检测Th9细胞比例、IL-9水平和转录因子PU.1 mRNA相对表达量。纯化肺癌组Th9细胞、CD8^+T细胞和原代NSCLC细胞,使用重组IL-9刺激CD8^+T细胞,观察细胞增殖和分泌穿孔素、颗粒酶B水平。建立Th9细胞、CD8^+T细胞与原代NSCLC细胞的共培养细胞,观察IL-9对CD8^+T细胞的细胞杀伤功能和非细胞杀伤功能的影响。组间比较采用t检验、配对t检验或Wilcoxon配对检验。结果:肺癌组外周血Th9细胞比例、IL-9水平和PU.1 mRNA相对表达量低于对照组(均P<0.0001),肺癌组肿瘤部位分离的BALF中Th9细胞比例、IL-9水平和PU.1 mRNA相对表达量低于非肿瘤部位(均P<0.0001)。IL-9刺激可增加肺癌组CD8^+T细胞增殖和穿孔素、颗粒酶B分泌,IL-9刺激还可提升肺癌组CD8^+T细胞对原代NSCLC细胞的细胞杀伤和非细胞杀伤功能,主要表现为细胞死亡比率增加、干扰素-γ和肿瘤坏死因子-α(TNF-α)分泌升高。Th9细胞也可提升肺癌组CD8^+T细胞对原代NSCLC细胞的细胞杀伤和非细胞杀伤功能,抗IL-9中和抗体可抑制Th9细胞对CD8^+T细胞功能的调控作用。结论:Th9细胞可能通过分泌IL-9在体外增强NSCLC患者CD8^+T细胞的抗肿瘤功能。

Super-resolution immunohistochemistry study on CD4 and CD8 cells and the relation to macrophages in human cochlea

Recently, the human cochlea has been shown to contain numerous resident macrophages under steady-state. The macrophages accumulate in the stria vascularis, among the auditory nerves, and are also spotted in the human organ of Corti. These macrophages may process antigens reaching the cochlea by invasion of pathogens and insertion of CI electrode. Thus, macrophages execute an innate, and possibly an adaptive immunity. Here, we describe the molecular markers CD4 and CD8 of T cells, macrophage markers MHCⅡ and CD11b, as well as the microglial markers TEME119 and P2Y12, in the human cochlea. Immunohistochemistry and the advantageous super-resolution structured illumination microscopy(SR-SIM) were used in the study. CD4^+ and CD8^+ cells were found in the human cochleae. They were seen in the modiolus in a substantial number adjacent to the vessels, in the peripheral region of the Rosenthal's canal, and occasionally in the spiral ligament. While there are a surprisingly large number of macrophages in the stria vascularis as well as between the auditory neurons,CD4^+ and CD8^+ cells are hardly seen in these areas, and neither are seen in the organ of Corti. In the modiolus,macrophages, CD4^+ and CD8^+ cells appeared often in clusters. Interaction between these different cells was easily observed with SR-SIM, showing closely placed cell bodies, and the processes from macrophages reaching out and touching the lymphocytes. Otherwise the CD4^+ and CD8^+ cells in human cochlear tissue are discretely scattered. The possible roles of these immune cells are speculated.

非小细胞肺癌患者外周血CD8^+和γδT细胞亚群表面PD1和BTLA的表达（英文）

Objective To investigate the expression and regulation of programmed cell death protein 1(PD1),B lymphocyte and T lymphocyte attenuator(BTLA)in peripheral blood of patients with non-small cell lung cancer(NSCLC);to examine the correlation of the mRNA levels between PD and BTLA in NSCLC.Methods Flow cytometry was used to detect the expression of PD1 and BTLA on the surfaces of CD8^+T cells andγδ+T cells in the peripheral blood samples collected from 32 in-patients with stage IV NSCLC and 30 healthy individuals.We compared the expression of PD1 and BTLA on the surfaces ofγδ+T cells in the NSCLC patients with bone metastasis before and after the treatment of zoledronic acid.The correlations of PD1 and BTLA,as well as their ligands were analyzed using Pearson correlation analysis with the cBioPortal data platform.Results The frequency of PD1 on the surfaces of CD8^+T cells was significantly higher than that of theγδT cells in both healthy controls(t=2.324,P=0.024)and NSCLC patients(t=2.498,P=0.015).The frequency of PD1 on CD8^+T cells,rather than onγδ+T cells,was significantly upregulated in advanced NSCLC patients compared with that in healthy controls(t=4.829,P<0.001).The PD1+BTLA+γδT cells of the healthy controls were significantly lower than that of the NSCLC patients(t=2.422,P=0.0185).No differences in percentage of PD1+γδ+and BTLA+γδ+T cells were observed in 7 NSCLC patients with bone metastasis before and after zoledronic acid treatment.PD1 was positively correlated with BTLA in both lung adenocarcinoma(r=0.54;P<0.05)and lung squamous cell carcinoma(r=0.78;P<0.05).Conclusions The upregulation of co-inhibitory molecules occurs on the surfaces of both CD8^+T cells andγδT cells in advanced NSCLC,suggesting that these molecules were involved in regulating the inactivation of CD8^+T cells andγδ+T cells,immune escape and tumor invasion.

Effects of pituitary adenylate cyclase activating polypeptide on CD4^+/CD8^+T cell levels after traumatic brain injury in a rat model

BACKGROUND:The effect of pituitary adenylate cyclase activating polypeptide(PACAP)during traumatic brain injury(TBI) and whether it can modulate secondary injury has not been reported previously.The present study evaluated the potential protective effects of ventricular infusion of PACAP in a rat model of TBI.METHODS:Male Sprague Dawley rats were randomly divided into 3 treatment groups(n=6,each):sham-operated,vehicle(normal saline)^+TBI,and PACAP^+TBI.Normal saline or PACAP(1 ug/5uL) was administered intracerebroventricularly 20 minutes before TBI.Right parietal cortical contusion was produced via a weight-dropping method.Brains were extracted 24 hours after trauma.Histological changes in brains were examined by HE staining.The numbers of CD4^+ and CD8^+ T cells in blood and the spleen were detected via flow cytometry.RESULTS:In injured brain regions,edema,hemorrhage,inflammatory cell infiltration,and swollen and degenerated neurons were observed under a light microscope,and the neurons were disorderly arrayed in the hippocampi.Compared to the sham group,average CD4^+ CD8" lymphocyte counts in blood and the spleen were significantly decreased in rats that received TBI^+vehicle,and CD4^+ CD8^+ were increased.In rats administered PACAP prior to TBI,damage was attenuated as evidenced by significantly increased CD4^+,and decreased CD8^+,T lymphocytes in blood and the spleen.CONCLUSION:Pretreatment with PACAP may protect against TBI by influencing periphery T cellular immune function.

Subgroups of peripheral immune effector cells in cervical cancer patients are more sensitive to radiation therapy than chemotherapy

Background:CD8 positive T lymphocytes and natural killer(NK)cells in the peripheral blood of cervical cancer patients exhibit varying sensitivities to radiotherapy and chemotherapy.Methods:A total of 50 healthy peoples and 60 cervical cancer patients were recruited.The patients with cervical cancer were separated into two groups:radiation and chemotherapy,and blood sample were collected before and after treatment.Data on the proportion of CD8 positive T lymphocytes and NK cells were gathered for analytical evaluation.Results:Compared to healthy individuals,patients with cervical cancer exhibit a reduced proportion of CD8 positive T cells within their peripheral blood.And for patients with cervical cancer,radiation therapy has been found to be more effective than chemotherapy in increasing the proportion of CD8 positive T lymphocytes and NK cells.Conclusions:These results suggest that radiation therapy increases the levels of CD8 positive T lymphocytes and NK cells within the peripheral blood of patients with cervical cancer.The study hypothesis that the changes in the percentage of CD8 positive T lymphocytes may serve as a potential indicator for predicting treatment efficacy.

绵阳市游仙区2019~2021年新报告HIV/AIDS患者首次T淋巴细胞检测结果分析

目的:分析绵阳市游仙区2019~2021年新报告艾滋病病毒感染者/艾滋病患者(HIV/AIDS)首次检测T淋巴细胞计数结果,为当地艾滋病防治提供依据。方法:通过回顾性分析研究252例新报告HIV/AIDS患者的CD^(+)_(3)T淋巴细胞(CD 3)、CD^(+)_(4)T淋巴细胞(CD 4)、CD^(+)_(8)T淋巴细胞(CD 8)检测结果,对不同时间、不同性别、不同年龄段患者的检测结果进行分析。结果:CD 3、CD 4、CD 8检测结果均较低。不同时间及不同性别HIV/AIDS病例CD^(+)_(3)、CD^(+)_(4)、CD^(+)_(8)检测结果均值无差异(均P>0.05),不同年龄CD 3、CD 4、CD^(+)_(8)检测结果均值存在差异(均P<0.05),>45岁病例均值最低。CD 4均值较低,主要集中在<500个/μL的范围。CD 4≤200个/μL的比例在历年来以及不同性别人群无差异(χ^(2)=0.454,0.256,均P>0.05),但不同年龄人群有差异(χ^(2)=12.474,P<0.05),在>45岁病例中占比最高。结论:绵阳市游仙区新报告HIV/AIDS患者T淋巴细胞计数均值低,晚发比例高,尤其>45岁人群。应加强该类人群的艾滋病宣教及健康筛查,并按要求及时治疗。

补肾方对慢性乙型肝炎T细胞亚群及其功能的影响

目的观察补肾方对慢性乙型肝炎患者免疫功能状态的调节作用。方法收集HBeAg阳性ALT异常者30例,给予补肾方治疗,用药6个月,于用药前、用药3个月、6个月时分别检测下列指标。采用罗氏荧光定量PCR法检测HBVDNA ,外周血单个核细胞(peripheralbloodmononuclearcell,PBMC)加入HBeAg、HBcAg与植物血凝素(Phytohemagglutinin ,PHA)培养48h ,检测培养上清液中IL 10、IFN -γ,血液中CD4+、CD8+、CD8+CD2 8+、CD8+CD2 8-和CD2 8+T细胞的比例及加入HBeAg、HBcAg与PHA培养48h后培养细胞中CD8+CD2 8+的比例。结果补肾方治疗有效组在治疗3个月后,PBMC培养中IFN- γ较治疗前明显上升,IL- 10明显下降(P <0 . 0 5,P <0. 0 1) ;CD8+CD2 8+T、CD2 8+T、培养后CD8+CD2 8+T细胞较治疗前明显上升,CD8+CD2 8-T细胞明显下降(P <0 .0 5或P <0 .0 1)。结论补肾方能明显增强Th1型细胞因子的表达,降低Th2型细胞因子的表达,促进CD8+(CytotoxicTlymphocyte ,CTL)的表达,这可能是使HBVDNA复制得到抑制的机制之一。

微波辐射损伤雷达作业人员外周血T淋巴细胞免疫活性

目的探讨微波辐射对雷达作业人员外周血T淋巴细胞免疫活性的影响。方法采取30例雷达作业人员和20例健康人的外周血,对外周血T淋巴细胞进行银染,检测淋巴细胞核仁银染强度;另外采用微量全血直接荧光法标记CD3+、CD4+、CD8+,用流式细胞仪检测T淋巴细胞亚群变化。结果巨嗜银核仁银染面积与细胞核银染面积的比值(I.S%),对照组为6.06±0.32,雷达作业组为5.12±0.57;对照组外周血CD3+、CD4+、CD8+百分比分别为(65.81±1.60)%、(32.58±0.71)%、(25.07±1.46)%,雷达作业组为(59.20±3.07)%、(28.32±2.66)%、(27.24±1.72)%。与对照组相比,雷达作业组外周血T淋巴细胞的I.S%明显降低(P<0.05),T淋巴细胞亚群CD3+、CD4+、CD4+/CD8+比值明显降低(P<0.05)。结论微波辐射对雷达作业人员的免疫活性产生明显影响,提示应加强雷达作业人员的防护措施。

乙型肝炎的T淋巴细胞亚群的演变规律

目的:了解不同类型乙型肝炎患者T淋巴细胞亚群的演变规律及意义。方法:我院2002年7月～2004年6月收治的16例急性肝炎、40例慢性肝炎及46例重型肝炎病例的抗凝血,通过流式细胞仪检测其T淋巴细胞亚群,对不同类型肝炎进行统计学分析。结果:慢性肝炎组与急性肝炎组比较CD3+,CD8+细胞计数降低且差异有显著性(P<0.05)。重型肝炎组与非重型肝炎组(急性肝炎组与慢性肝炎组)比较CD3+,CD4+,CD8+细胞计数明显降低(P<0.05),幼稚的CD4+,CD8+细胞计数明显升高(P<0.05)。结论:不同类型乙型肝炎的T淋巴细胞亚群有明显差异,乙型肝炎随着病情严重及病程延长,CD3+,CD4+,CD8+淋巴细胞逐渐减少,重型肝炎出现大量幼稚的CD4+CD8+淋巴细胞,T淋巴细胞亚群的变化可以反映病情严重程度及免疫状态,对免疫调节治疗有指导意义。

靶向T细胞肿瘤治疗临床进展

T淋巴细胞在抗肿瘤免疫中发挥重要作用。根据细胞表面CD分子的不同，T细胞可分为CD4＋T细胞和CD8＋T细胞。CD4＋T细胞活化后的主要效应细胞为辅助性T细胞（help T lymphocyte，Th），CD8＋T细胞活化后的主要效应细胞为细胞毒性T细胞（cytotoxicTlymphocyte，CTL）。CTL对肿瘤细胞起直接杀伤作用。Th对CTL的免疫活性起调节和促进作用。

Role of CD8^(+) T lymphocyte cells:Interplay with stromal cells in tumor microenvironment

CD8^(+) T lymphocytes are pivotal cells in the host response to antitumor immunity.Tumordriven microenvironments provide the conditions necessary for regulating infiltrating CD8^(+) T cells in favor of tumor survival,including weakening CD8^(+) T cell activation,driving tumor cells to impair immune attack,and recruiting other cells to reprogram the immune milieu.Also in tumor microenvironment,stromal cells exert immunosuppressive skills to avoid CD8^(+) T cell cytotoxicity.In this review,we explore the universal function and fate decision of infiltrated CD8^(+) T cells and highlight their antitumor response within various stromal architectures in the process of confronting neoantigen-specific tumor cells.Thus,this review provides a foundation for the development of antitumor therapy based on CD8^(+)T lymphocyte manipulation.

基于T淋巴细胞亚群探析中医药治疗老年肺炎研究现状

T淋巴细胞亚群是一群在相同时期、处于不同发育阶段的异质性细胞的总称,具有抵抗病毒和调节免疫系统功能的作用。老年肺炎病情重,并发症多,病死率高,多与老年人免疫功能相对低下有关,与中医的正气不足有关,T淋巴细胞亚群与老年肺炎存在相关性,且与中医正气有共通之处,因此可基于T淋巴细胞亚群探析老年人正气不足的原因并指导临床,进一步对中医药治疗老年肺炎进行探究,以期提高临床诊疗水平,减轻患者负担。

Key role of human leukocyte antigen in modulating human immunodeficiency virus progression: An overview of the possible applications

Host and viral factors deeply influence the human immunodeficiency virus(HIV) disease progression. Among them human leukocyte antigen(HLA) locus plays a key role at different levels. In fact, genes of the HLA locus have shown the peculiar capability to modulate both innate and adaptive immune responses. In particular, HLA class Ⅰmolecules are recognized by CD8+ T-cells and natural killers(NK) cells towards the interaction with T cell receptor(TCR) and Killer Immunoglobulin Receptor(KIR) 3DL1 respectively. Polymorphisms within the different HLA alleles generate structural changes in HLA classⅠpeptide-binding pockets. Amino acid changes in the peptide-binding pocket lead to the presentation of a different set of peptides to T and NK cells. This review summarizes the role of HLA in HIV progression toward acquired immunodeficiency disease syndrome and its receptors. Recently, many studies have been focused on determining the HLA binding-peptides. The novel use of immune-informatics tools, from the prediction of the HLA-bound peptides to the modification of the HLAreceptor complexes, is considered. A better knowledge of HLA peptide presentation and recognition are allowing new strategies for immune response manipulation to be applied against HIV virus.

肺癌患者外周血T淋巴细胞亚群检测的临床研究

通过流式细胞仪对确诊原发性支气管肺癌患者外周血T淋巴细胞亚群检测研究 ,并与健康人作对比。结果显示 ,肺癌患者CD3 + ( 68 88± 11 80 )和CD4+ ( 3 8 83± 10 3 0 )以及CD4+ /CD8+ ( 1 5 3± 0 74)比例均比正常人 ( 74 2 1±5 5 1,43 92± 7 0 2 ,1 89± 0 5 4)下降 ,CD8+ ( 2 9 2 6± 10 5 8)高于正常人 ( 2 4 5 7± 6 3 5 ) ,两组之间差异有统计学意义 ,P均 <0 0 5。肿瘤临床分期之间检测结果差异无统计学意义 ,而病理类型中未分化癌与鳞癌之间CD4+ 和CD4+ /CD8+下降具有统计学意义。初步研究结果提示 ,原发性支气管肺癌患者存在细胞免疫功能低下 ,并与细胞分化类型有关。

T lymphocyte apoptosis induced by CD8ε chimera

THE T cell repertoire is characterized by an extremely diverse population of different surface receptors (TCR) which participates in highly specific responses to a large number of different antigens and mediate antigen stimulation signals. Variable heterodimers of TCR α/β and γ/δ receptors are associated with invariable CD3γ, δ, ε and ζ proteins, thus forming the TCR/CD3 complex. CD3ε plays an important role in the initiation of TCR resembling and signfal transduction among the octopeptide chains. Stimulation by anti-CD3ε monoclonal

MELATONIN AND IMMUNOMODULATION IN AGED AND IMMUNODEFICIENT MICE

Objective To investigate melatonin-related mechanisms of action on immunoregulation in aged and immunodeficient mice. Methods T lymPhocytes subunit CD4 + ,CD8 + and CD4 + /CD8 + ratio were measured by Flow Cytometer in normal, aged and Cyclophosphamide injected mice which treated with melatonin, and compared with the results of T lymphocytes subunit in the group without melatonin as control group. Results The percentage of CD4 + , CD8 + T cells in the normal mice which treated with melatonin was significantly higher than that in control group ( P <0.01), CD4 + /CD8 + ratio was higher but had no significant difference. In the cyclophosphamide injected group which melatonin treated, the percentage of CD4 + T cells and CD4 + /CD8 + ratio were higher than those in control, The difference was significant ( P <0.01), while CD8 + was lower ( P <0.01). In aged melatonin treated mice group, the percentage of CD4 + , CD8 + T cells and CD4 + /CD8 + ratio were significantly higher than those in control ( P <0.01). Conclusion Melatonin could adjust the quantity and the ratio of CD4 + , CD8 + T cells in aged and immunodeficient mice. it implied that melatonin could mediate helper and suppression T lymphocytes to reinforce their immunodefence.