目的探讨细胞分裂周期蛋白20(cell division cycle 20,CDC20)在子宫内膜癌中的表达及意义。方法选取2020年1月—2021年3月于齐齐哈尔医学院附属第三医院妇产科收治入院的96例子宫内膜癌患者为研究对象,采用免疫组化法和实时荧光定量聚...目的探讨细胞分裂周期蛋白20(cell division cycle 20,CDC20)在子宫内膜癌中的表达及意义。方法选取2020年1月—2021年3月于齐齐哈尔医学院附属第三医院妇产科收治入院的96例子宫内膜癌患者为研究对象,采用免疫组化法和实时荧光定量聚合酶链反应法检测CDC20水平。根据子宫内膜癌组织中CDC20表达水平将其分为CDC20高表达组及CDC20低表达组,对比两组临床病理特征及分析子宫内膜癌患者CDC20表达情况的影响因素分析。结果96例子宫内膜癌组织中CDC20高表达69例,低表达27例。两组年龄、体质指数、分娩次数、分娩方式比较,差异无统计学意义(P>0.05);两组组织学分型、国际妇产科联盟(Federation International of Gynecology and Obstetrics,FIGO)分期、病理分级、CDC20mRNA及CDC20蛋白评分比较,差异有统计学意义(P<0.05)。经Cox回归分析显示,子宫内膜癌患者CDC20表达情况的影响因素是FIGO分期(OR=2.604)、病理分级(OR=1.559)、CDC20mRNA(OR=4.198)、CDC20蛋白(OR=2.265)。结论CDC20在子宫内膜癌中呈高表达,FIGO分期、病理分级、CDC20mRNA、CDC20蛋白是子宫内膜癌患者CDC20表达情况的影响因素。展开更多
Objective:to study the expression and clinical significance CDC20,TOP2A,NEK2 esophageal squamous cell carcinoma.Methods:To select 70 patients with esophageal squamous cell carcinoma,Between August 2018-August 2020,All...Objective:to study the expression and clinical significance CDC20,TOP2A,NEK2 esophageal squamous cell carcinoma.Methods:To select 70 patients with esophageal squamous cell carcinoma,Between August 2018-August 2020,All intraoperative pathological specimens,A group-35 cases),Cancer tissue,B group,adjacent tissues),two groups of CDC20,TOP2A,NEK 2 expression were detected and analyzed by immunohistochemistry and semi-quantitative reverse transcription polymerase chain reaction-RT-PcR)assay.Results:the values of CDC20,TOP2A,NEK2 expression level in A group were significantly higher than those in B group-P<0.05).The expression level CDC20,TOP2A,NEK2 esophageal squamous cell carcinoma was positively correlated with TNM stage and lymphatic metastasis,and negatively correlated with tumor differentiation.Conclusion:CDC20,TOP2A,NEK2 high expression level directly affects the metastasis,recurrence and prognosis of esophageal squamous cell carcinoma.The combination of three indexes can accurately evaluate the pathological status of patients with esophageal squamous cell carcinoma and help to judge the prognosis of patients accurately.展开更多
Objective:To study the expression of CDC20,TOP2A in esophageal squamous cell carcinoma and its relationship with survival of esophageal carcinoma.Methods:65 patients with esophageal squamous cell carcinoma from Januar...Objective:To study the expression of CDC20,TOP2A in esophageal squamous cell carcinoma and its relationship with survival of esophageal carcinoma.Methods:65 patients with esophageal squamous cell carcinoma from January 2016 to June 2018 were selected by computer random selection.All patients were treated with radical operation.The CDC20,TOP2A expression of the patients was examined.At the same time,the relationship between 3-year survival rate and CDC20,TOP2A was analyzed by follow-up investigation.Results:the CDC20,TOP2A expression level of cancer tissue group was higher than that of adjacent tissue group and normal tissue group(P<0.05),and the CDC20,TOP2A expression level of adjacent tissue group was higher than that of normal tissue group(P<0.05).There was no significant difference in sex,age,tumor size,tumor location and CDC20,TOP2A expression level in patients with esophageal squamous cell carcinoma,P>0.05;there were differences between groups(P P>0.05)and positive proportion in TNM stage,lymphatic metastasis,invasion factor and CDC20,TOP2A expression level;there were differences in tumor differentiation,5-year survival rate and CDC20,TOP2A expression level(P P>0.05),and showed inverse proportion relationship.Conclusion:metastasis,recurrence and prognosis of esophageal squamous cell carcinoma are related to the level of CDC20,TOP2A expression.These two indexes can effectively evaluate the pathological situation of esophageal cancer and provide an important reference for the prognosis of esophageal squamous cell carcinoma patients.展开更多
文摘目的探讨细胞分裂周期蛋白20(cell division cycle 20,CDC20)在子宫内膜癌中的表达及意义。方法选取2020年1月—2021年3月于齐齐哈尔医学院附属第三医院妇产科收治入院的96例子宫内膜癌患者为研究对象,采用免疫组化法和实时荧光定量聚合酶链反应法检测CDC20水平。根据子宫内膜癌组织中CDC20表达水平将其分为CDC20高表达组及CDC20低表达组,对比两组临床病理特征及分析子宫内膜癌患者CDC20表达情况的影响因素分析。结果96例子宫内膜癌组织中CDC20高表达69例,低表达27例。两组年龄、体质指数、分娩次数、分娩方式比较,差异无统计学意义(P>0.05);两组组织学分型、国际妇产科联盟(Federation International of Gynecology and Obstetrics,FIGO)分期、病理分级、CDC20mRNA及CDC20蛋白评分比较,差异有统计学意义(P<0.05)。经Cox回归分析显示,子宫内膜癌患者CDC20表达情况的影响因素是FIGO分期(OR=2.604)、病理分级(OR=1.559)、CDC20mRNA(OR=4.198)、CDC20蛋白(OR=2.265)。结论CDC20在子宫内膜癌中呈高表达,FIGO分期、病理分级、CDC20mRNA、CDC20蛋白是子宫内膜癌患者CDC20表达情况的影响因素。
文摘Objective:to study the expression and clinical significance CDC20,TOP2A,NEK2 esophageal squamous cell carcinoma.Methods:To select 70 patients with esophageal squamous cell carcinoma,Between August 2018-August 2020,All intraoperative pathological specimens,A group-35 cases),Cancer tissue,B group,adjacent tissues),two groups of CDC20,TOP2A,NEK 2 expression were detected and analyzed by immunohistochemistry and semi-quantitative reverse transcription polymerase chain reaction-RT-PcR)assay.Results:the values of CDC20,TOP2A,NEK2 expression level in A group were significantly higher than those in B group-P<0.05).The expression level CDC20,TOP2A,NEK2 esophageal squamous cell carcinoma was positively correlated with TNM stage and lymphatic metastasis,and negatively correlated with tumor differentiation.Conclusion:CDC20,TOP2A,NEK2 high expression level directly affects the metastasis,recurrence and prognosis of esophageal squamous cell carcinoma.The combination of three indexes can accurately evaluate the pathological status of patients with esophageal squamous cell carcinoma and help to judge the prognosis of patients accurately.
文摘Objective:To study the expression of CDC20,TOP2A in esophageal squamous cell carcinoma and its relationship with survival of esophageal carcinoma.Methods:65 patients with esophageal squamous cell carcinoma from January 2016 to June 2018 were selected by computer random selection.All patients were treated with radical operation.The CDC20,TOP2A expression of the patients was examined.At the same time,the relationship between 3-year survival rate and CDC20,TOP2A was analyzed by follow-up investigation.Results:the CDC20,TOP2A expression level of cancer tissue group was higher than that of adjacent tissue group and normal tissue group(P<0.05),and the CDC20,TOP2A expression level of adjacent tissue group was higher than that of normal tissue group(P<0.05).There was no significant difference in sex,age,tumor size,tumor location and CDC20,TOP2A expression level in patients with esophageal squamous cell carcinoma,P>0.05;there were differences between groups(P P>0.05)and positive proportion in TNM stage,lymphatic metastasis,invasion factor and CDC20,TOP2A expression level;there were differences in tumor differentiation,5-year survival rate and CDC20,TOP2A expression level(P P>0.05),and showed inverse proportion relationship.Conclusion:metastasis,recurrence and prognosis of esophageal squamous cell carcinoma are related to the level of CDC20,TOP2A expression.These two indexes can effectively evaluate the pathological situation of esophageal cancer and provide an important reference for the prognosis of esophageal squamous cell carcinoma patients.