Effect of Naojian Tablet on cerebral ischemic injury and its prevention mechanism

OBJECTIVE To investigate the prevention and its mechanism of Naojian Tablet,the adjusted prescription from Buyang Huanwu Decoction,during focal ischemic brain injury condition,and study on the main bioactive substance for this function.METHODS To apply the modified middle cerebral artery occlusion(MCAO)model in rats,immunohistochemistry,RT-PCR and Western blotting were applied to determine the expression of CDK5,CDK4/cyclinD1 in the hippocampus tissues in MCAO rat by treating with Naojian Tablet.On the other hand,the processes of metabolism and disposition of several active components in Naojian Tablet and Buyang Huanwu Decoction were analyzed and characterized by using HPLC-Q-TOF method.RESULTS Naojian Tablet(ig,2.41g·kg-1)could significantly decrease the levels of CDK5,CDK4/cyclinD1 in hippocampus tissues in MACO rat after treated by 3d,7d,14 d,28d(P<0.05).The similar mechanism was observed in the Buyang Huanwu Decoction treated MACO rat.Calycosin,fermononetin,senkyunolide and ligustilide in Naojian Tablet was determined simultaneously as the main active ingredient of Naojian Tablet.CONCLUSION The above-mentioned in vivo studies suggested that Naojian Tablet maybe play its prevention effect on nerve cells via its acting on CDK5 signaling and cell cycle pathway,which verified the multi-target and multi active pathways efficiency of tradition Chinese medicine in treating diverse disease.