克隆新基因及获得其 c DNA全长是现代生命科学中研究基因功能的重要前提。随着分子生物学技术的迅速发展 ,克隆新基因的技术也不断得到发展和完善 ,尤其是生物技术与信息技术的结合和应用 ,计算机克隆基因开始兴起。本文介绍几种近年常...克隆新基因及获得其 c DNA全长是现代生命科学中研究基因功能的重要前提。随着分子生物学技术的迅速发展 ,克隆新基因的技术也不断得到发展和完善 ,尤其是生物技术与信息技术的结合和应用 ,计算机克隆基因开始兴起。本文介绍几种近年常用的克隆新基因 c DNA全长的方法。展开更多
As a high-grade edible oil tree native in China,tea-oil tree(Camellia oleifera)has the oil-yielded rate of about 55% from its kernel.The recent researches suggested that tea-oil would be one of the best vegetable oils...As a high-grade edible oil tree native in China,tea-oil tree(Camellia oleifera)has the oil-yielded rate of about 55% from its kernel.The recent researches suggested that tea-oil would be one of the best vegetable oils,and even be better than olive oil with its abundant unsaturated fatty acids including 82.6% oleic acid.Stearoyl-ACP desaturase(SAD)is a key enzyme that catalyzes saturated fatty acids(C18∶0)bonded to ACP(Acyl carrier protein)and dehydrogenates the fatty acids into oleic acids,and hence controls the content of oleic acid and the proportion between saturated fatty acids and unsaturated fatty acids.With our previous acquisition of three cDNAs and ESTs of C.oleifera SAD(CoSAD)gene,5’RACE technology was used to obtain the full-length cDNA of CoSAD gene from the nearly matured C.oleifera seed.The comprehensive bioinformatic analyses including sequence characteristics of DNA and amino acid,multi-sequence aligning,identity and homology,molecular clustering,protein physicochemical properties,and protein structural prediction and characteristics were performed.The results may provide the theoretical and material elements for application of CoSAD gene and genetic improvement on other oil plants.展开更多
文摘As a high-grade edible oil tree native in China,tea-oil tree(Camellia oleifera)has the oil-yielded rate of about 55% from its kernel.The recent researches suggested that tea-oil would be one of the best vegetable oils,and even be better than olive oil with its abundant unsaturated fatty acids including 82.6% oleic acid.Stearoyl-ACP desaturase(SAD)is a key enzyme that catalyzes saturated fatty acids(C18∶0)bonded to ACP(Acyl carrier protein)and dehydrogenates the fatty acids into oleic acids,and hence controls the content of oleic acid and the proportion between saturated fatty acids and unsaturated fatty acids.With our previous acquisition of three cDNAs and ESTs of C.oleifera SAD(CoSAD)gene,5’RACE technology was used to obtain the full-length cDNA of CoSAD gene from the nearly matured C.oleifera seed.The comprehensive bioinformatic analyses including sequence characteristics of DNA and amino acid,multi-sequence aligning,identity and homology,molecular clustering,protein physicochemical properties,and protein structural prediction and characteristics were performed.The results may provide the theoretical and material elements for application of CoSAD gene and genetic improvement on other oil plants.
