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Effects of CD11/CD18 mediated PMN-EC adhesion on the endothelial cell damages in the early stage postburn in vitro
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作者 李志清 杨宗城 +2 位作者 罗向东 罗勤 黎鳌 《Journal of Medical Colleges of PLA(China)》 CAS 1997年第2期88-92,共5页
Objective: To investigate the effects of CD11/CD18 on the endothelial cell (EC)damages induced by polymorphonuclear neutrophils (PMN )from patients in the early stage postburn. Metbods: PMNs from 10 patients in the fi... Objective: To investigate the effects of CD11/CD18 on the endothelial cell (EC)damages induced by polymorphonuclear neutrophils (PMN )from patients in the early stage postburn. Metbods: PMNs from 10 patients in the first 24 to 48 h postburn were incubated with cultured human umbilical vein ECs (HUVECs) for 24 h and the percentage of PMN-EC adhesion was calculated, the content of lactate dehydrogenase (LDH), angiotensin converting enzyme (ACE) and 6-keto-PGF,. in the medium determined and the morphological changes Qf ECs observed. Results: After incubation with PMNs from burn victims, deformation of the HUVECs cell shrinkage, and formation of intercellurar space occurred and some cells were detached from the vascular wall. The content of LDH, ACE and 6-keto-PGF,. increased. When the PMNs were pretreated with monoclonal antibodies (McAbs)against CD11a/CD11b or CDl1b/CD18, the PMN-EC adhesion rate and the content of LDH, ACE and 6-ketoPGF,. in the culture medium decreased and Ec damages attenuated especially when the 2 kinds of McAbs were used in combination. Conclusion: The findings indicate that in the early stage postburn, PMNs can induce damages of ECs which results from CD1l/CD18 mediated PMN-CE adhesion and that the administration of McAbs against CD11/CD18 mediated PMN-EC adhesion and that the administration of McAbs against CD11/CD18 is able to b1ock partially the PMN-EC adhesion in burn patients and attenuate the damage of ECs. 展开更多
关键词 ENDOTHELIAL cell POLYMORPHONUCLEAR NEUTROPHIL cdll/cd18 damage
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严重烧伤大鼠早期外周血中性粒细胞CD11b/CD18分子的动态变化 被引量:5
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作者 方勇 陈玉林 +3 位作者 葛绳德 夏照帆 张素贞 蔡清萍 《中华整形烧伤外科杂志》 CSCD 北大核心 1997年第2期86-88,共3页
为探讨烧伤后早期外周血中性粒细胞(PMN)膜上 CD11b/CD18分子的动态变化,采用大鼠30%TBSAⅢ度烧伤模型,伤后随机分为立即复苏组和延迟复苏组,于伤前及伤后12小时内的不同时间取血作流式细胞仪分析。结果表明:两组大鼠于伤后30分钟起 PMN... 为探讨烧伤后早期外周血中性粒细胞(PMN)膜上 CD11b/CD18分子的动态变化,采用大鼠30%TBSAⅢ度烧伤模型,伤后随机分为立即复苏组和延迟复苏组,于伤前及伤后12小时内的不同时间取血作流式细胞仪分析。结果表明:两组大鼠于伤后30分钟起 PMN CD11b/CD18表达就明显增加;立即复苏组于伤后4小时达到峰值;延迟复苏组在伤后1小时和8小时各有一个峰值,并比立即复苏组增高。认为烧伤后粘附分子 CD11b/CD18的变化可能与炎性介质、细胞因子、血流动力学改变引起的PMN 切应力的变化及缺血再灌注等密切相关。 展开更多
关键词 细胞粘附 cd11/cd18 烧伤
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